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河南省人源和食源多重耐药致泻大肠埃希氏菌耐药特征与基因组分析

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中国人兽共患病学报 2024年第8期40卷 723-731页

作者：戚浩彧李艳芬王钰邱正勇崔莹吴玲玲张濛李永利河南省疾病预防控制中心郑州450016

目的了解河南省临床和食品来源中多重耐药的致泻大肠埃希氏菌的耐药性和基因组特征.方法对2017-2022年河南省分离的101株多重耐药致泻大肠埃希氏菌,通过微量肉汤稀释法进行抗生素敏感性实验.基于全基因组测序结果分析其耐药基因、多位... 详细信息

目的了解河南省临床和食品来源中多重耐药的致泻大肠埃希氏菌的耐药性和基因组特征.方法对2017-2022年河南省分离的101株多重耐药致泻大肠埃希氏菌,通过微量肉汤稀释法进行抗生素敏感性实验.基于全基因组测序结果分析其耐药基因、多位点序列分型、质粒类型并基于核心基因组单核苷酸多态性位点(cgSNPs)构建系统发育树.结果101株多重耐药的致泻大肠埃希氏菌对氨苄西林、四环素、萘啶酸的耐药率最高,分别为87.1%、79.2%、64.4%;对头孢噻肟的耐药率为38.6%.全基因组测序数据表明:101株分离株可被分为60种ST型,其中ST10、ST1491、ST38是优势型别;101株分离株共预测出23种质粒类型,其中携带率最高的类型为IncFIB.分离株携带喹诺酮类、氨基糖苷类、βG内酰胺酶类、四环素类等多种耐药基因,其中超广谱βG内酰胺酶基因bla_(CTX-M-14)和bla_(CTX-M-55)上下游遗传环境中均存在插入序列(IS26、IS903B、ISECP1).结论河南省人源和食源多重耐药的致泻大肠埃希氏菌具有较高的遗传多样性,整体耐药水平较高,其bla_(CTX-M)基因的传播可能与插入序列(IS)有关.

关键词：多重耐药致泻大肠埃希氏菌全基因组测序耐药性 bla_(CTX-M)

染色体微阵列分析和全外显子组测序在产前罕见疾病诊断中的应用

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山东医学高等专科学校学报 2024年第1期46卷 7-10页

作者：李永丽李华锋临沂市妇幼保健院山东临沂276000

目的探讨产前罕见疾病的诊断方法。方法选取4845例有产前诊断指征的孕妇,所有胎儿均行CMA检测CNV,在排除非整倍体和异常CNV后,其中68例进一步行WES检测。结果4842例样本CMA检测成功,检出染色体异常537例(11.09%),经分析明确致病性变异为... 详细信息

目的探讨产前罕见疾病的诊断方法。方法选取4845例有产前诊断指征的孕妇,所有胎儿均行CMA检测CNV,在排除非整倍体和异常CNV后,其中68例进一步行WES检测。结果4842例样本CMA检测成功,检出染色体异常537例(11.09%),经分析明确致病性变异为370例,包括:非整倍体214例,致病性CNV 134例和部分CNV嵌合22例。疾病主要涉及1q21缺失综合征(7例)、DiGeorge综合征(6例)、17q12缺失综合征(6例)、Cri-du-chat综合征(5例)、16p11.2缺失综合征(5例)。WES结果显示在68例CMA结果阴性的胎儿中检出13例单基因病(19.12%)。结论CMA和WES是明确产前胎儿遗传病因和产前诊断的有效手段。

关键词：染色体微阵列分析全外显子组测序产前诊断基因变异

高职院校“双师型”教师专业发展的内生动力及培育路径

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职业教育研究 2024年第7期 58-63页

作者：徐紫娟李永利西北师范大学甘肃兰州730070 包头医学院内蒙古包头014060

高职院校“双师型”教师专业发展既是顺应高等职业教育高质量发展的必然选择,也是满足高职院校“双师型”教师个人职业发展需求的有效途径。助力高职院校“双师型”教师专业发展的关键在于激发教师的内生动力。聚焦高职院校“双师型”... 详细信息

高职院校“双师型”教师专业发展既是顺应高等职业教育高质量发展的必然选择,也是满足高职院校“双师型”教师个人职业发展需求的有效途径。助力高职院校“双师型”教师专业发展的关键在于激发教师的内生动力。聚焦高职院校“双师型”教师专业发展内生动力的内涵及构成,从身份认同、专业情怀和主体意识三个向度阐明“双师型”教师专业发展内生动力的现实困境,提出通过文化建构,增强高职院校“双师型”教师身份认同;理念转变,提升高职院校“双师型”教师专业情怀;行动反思,唤醒高职院校“双师型”教师主体意识,从而有效培育高职院校“双师型”教师专业发展的内生动力。

关键词：高职院校 “双师型”教师专业发展内生动力

Purification of specularite by centrifugation instead of flotation to produce iron oxide red pigment

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International Journal of Minerals,Metallurgy and Materials 2021年第1期28卷 56-65页

作者： Xi Zhang Shun-wei Zhu Yu-jiang li yong-li li Qiang Guo Tao Qi National Engineering Laboratory of Hydrometallurgical Cleaner Production Technology Institute of Process EngineeringChinese Academy of SciencesBeijing 100190China School of Environmental Science and Engineering Shandong UniversityQingdao 266200China Zhengzhou Institute of Emerging Industrial Technology Zhengzhou 450003China

This study used specularite, a high-gradient magnetic separation concentrate, as a raw material in reverse *** iron concentrate with a grade of 65.1 wt% and a recovery rate of 75.31% were obtained.A centrifugal concentrator served as the deep purification equipment for the preparation of iron oxide red pigments, and its optimal rotating drum speed, feed concentration, and other conditions were *** optimal conditions, a high-purity iron oxide concentrate with a grade of 69.38 wt% and a recovery rate of 80.89% were obtained and used as a raw material for preparing iron oxide red *** with sulfuric acid produced iron red pigments with different ***, middlings with a grade of 60.20 wt% and a recovery rate of 17.51% were obtained and could be used in blast furnace ***-value utilization of specularite beneficiation products was thus achieved.

关键词： specularite reconcentration centrifugal concentrator iron oxide red pigment

Influence of Na_2CO_3 as Additive on Direct Reduction of Boron-bearing Magnetite Concentrate

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Journal of Iron and Steel Research International 2016年第2期23卷 103-108页

作者： yong-li li Jing-kui QU Guang-ye WEI Tao QI National Engineering Laboratory for Hydrometallurgical Cleaner Production Technology Institute of Process EngineeringChinese Academy of Sciences Key Laboratory of Green Process and Engineering Institute of Process EngineeringChinese Academy of Sciences

Boron-bearing magnetite concentrate is typically characterized by low grade of iron and boron （wTFe = 51%- 54%, WB2O3 =6%-8%）, as well as the close intergrowth of ascharite phase and magnetite phase. A promising technology was proposed to separate iron and boron by coupling the direct reduction of iron oxides and Na activation of boron minerals together. The influence of Na2CO3 as additive on the direct reduction of boron-bearing magnetite was studied by chemical analysis, kinetic analysis, XRD analysis and SEM analysis. The results showed that the ad- dition of Na2CO3 not only activated boron minerals, but also reduced the activation energy of the reaction and pro- moted the reduction of iron oxides. Besides, the addition of Na2CO3 changed the composition and melting point of non-ferrous phase, and then promoted the growth and aggregation of iron grains, which was conducive to the subse- quent magnetic separation. Thus, the coupling of the two processes is advantageous,

关键词： ludwigite kinetic analysis direct reduction magnetic separation sodium carbonate

Relationship Between Differential Interference Angle and Parameter of Experiment in Molecular Beam

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Communications in Theoretical Physics 2006年第2X期46卷 294-296页

作者： li yong-Qing li Jian MA Feng-Cai Department of Physics Liaoning University Shenyang 110036 China

Collisional quantum interference （CQI） was observed in the intramolecular rotational energy transfer in the experiment of the static cell, and the integral interference angles were measured. To observe more precise information, the experiment in the molecular beam should be taken,from which the relationship between the differential interference angle and the scattering angle can be obtained. In this paper, the theoretical model of CQI is described in an atom-diatom system in the condition of the molecular beam, based on the first-Born approximation of time-dependent perturbation theory, taking into accounts the long-range interaction potential. The method of observing and measuring correctly the differential interference angle is presented. The changing tendency of the differential interference angle with the impact parameter and relative velocity is discussed. The changing tendencies of the differential interference angle with the parameter of experiment in the molecular beam, including the impact parameter and the velocity are discussed. This theoretical model is important to understand or perform the experiment in the molecular beam.

关键词： differential interference angle quantum interference rotational energy transfer

Novel Synthetic Method for Triacetylbenzene

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Chinese Chemical Letters 2006年第9期17卷 1165-1168页

作者： Bin ZHANG yong GAO Jian li li Zhen SHI Department of Chemistry Northwest University Xi＇an 710069

Triacetylbenzene was prepared from tribenzimidazolium salt and Grignard reagent. The addition-hydrolysis reaction of tribenzimidazolium salt with Grignard reagent is reported and a novel method for preparation of tria... 详细信息

关键词： Triacetylbenzene tribenzimidazolium salt Grignard reagent synthesis.

A new trimeric furanocoumarin from Heracleum rapula

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Chinese Chemical Letters 2009年第9期20卷 1088-1090页

作者： Cun Zhang YuanYan liu yong Qing Xiao li li Institute of Chinese Materia Medica China Academy of Traditional Chinese Medicine Beijing 100700 China

A new trimeric linear-type furanocoumarin, rapultririn A （1）, was isolated from the underground part ofHeracleum rapula. The structure of this new compound was elucidated by NMR, MS spectroscopic methods. 2009 Yon... 详细信息

关键词： Heracleum rapula Tricoumarin Rapultririn A

Two new glycosides from the seeds of Cassia obtusifolia

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Chinese Chemical Letters 2009年第9期20卷 1097-1099页

作者： Cun Zhang Gui liu li yong Qing Xiao li li Zhen Pang Institute of Traditional Chinese Materia Madica Chinese Academy of Chinese Medical Sciences Beijing 100700 China

Two new glycosides, nor-rubrofusadn-6-O-β-D-（6＇-O-acetyl）glucopyranoside （1） and 1-desmethylaurantio-obtusin-2-O-β-D- glucopyranoside （2） were isolated from the seeds of Cassia obtusifolia and their structures were established by chemical and spectral evidences. 2009 yong Qing Xiao. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.

关键词： Cassia obtusifolia Nor-rubrofusarin-6-O-β-D-(6'-O-acetyl)glucopyranoside 1-Desmethylaurantio-obtusin-2-O-β-D-glucopyranoside