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Alisol B acetate induces apoptosis of SGC7901 cells via mitochondrial and phosphatidylinositol 3-kinases/Akt signaling pathways

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World Journal of Gastroenterology 2009年第23期15卷 2870-2877页

作者： yong-hong xu li-jie zhao yan li Department of DigestiveDiseases Shengjing Hospital of China Medical UniversityShenyang 110004 Liaoning Province China

AIM： To examine the effect of alisol B acetate on the growth of human gastric cancer cell line SGC7901 and its possible mechanism of action.METHODS： The cytotoxic effect of alisol B acetate on SGC7901 cells was measured by 3-（4,5-dimethylthiazol- 2-yl）-2,5-diphenyltetrazolium bromide （MI-I-） assay. Phase-contrast and electron microscopy were used to observe the morphological changes. Cell cycle and mitochondrial transmembrane potential （A^Pm） were determined by flow cytometry. Western blotting was used to detect the expression of apoptosis-regulated gene Bcl-2, Bax, Apaf-1, caspase-3, caspase-9, Akt, P-Akt and phosphatidylinositol 3-kinases （PI3K）.RESULTS： Alisol B acetate inhibited the proliferation of SGC7901 cell line in a time- and dose-dependent manner. PI staining showed that alisol B acetate can change the cell cycle distribution of SGC7901, increase the proportion of cells in G0-G1 phase and decrease the proportion of S phase cells and G2-M phase cells. Alisol B acetate at a concentration of 30 pmol/L induced apoptosis after 24, 48 and 72 h incubation, with occurrence rates of apoptotic cells of 4.36%, 14.42% and 21.16%, respectively. Phase-contrast and electron microscopy revealed that the nuclear fragmentation and chromosomal condensed, cells shrank and attachment loss appeared in the SGC7901 treated with alisol B acetate. Apoptosis of SGC7901 cells was associated with cell cycle arrest, caspase-3 and caspase-9 activation, loss of mitochondrial membrane potential and up-regulation of the ratio of Bax/Bcl-2 and inhibition of the PI3K/Akt.CONCLUSION： Alisol B acetate exhibits an antiproliferative effect in SGC7901 cells by inducing apoptosis. Apoptosis of SGC7901 cells involves mitochondria-caspase and PI3K/Akt dependent pathways.

关键词： Alisol B acetate Apoptosis Mitochondria Phosphatidylinositol 3-kinases/Akt SGC7901 cells

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Recent progress on photoactive nonprecious transition-metal complexes

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Science China Chemistry 2025年第1期68卷 46-95页

作者： Zhong-liang Gong hong-jie Zhang yan Cheng Jianxun liu Yuhan Ai Yaqin li Zhihui Feng Qiong Zhang Shaolong Gong yong Chen Chang-Jiang Yao Yuan-Yuan Zhu liang-Jin xu Yu-Wu Zhong CAS Key Laboratory of Photochemistry Institute of Chemistry Chinese Academy of Sciences State Key Laboratory of Structural Cemistry Fujian Institute of Research on the Structure of Matter Chinese Academy of Sciences School of Chemistry and Chemical Engineering Anhui Key Laboratory of Advanced Catalytic Materials and Reaction EngineeringHefei University of Technology State Key Laboratory of Explosion Science and Safety Protection School of Mechatronical Engineering Beijing Institute of Technology College of Chemistry and Molecular Sciences Hubei Key Lab on Organic and Polymeric Optoelectronic Materials Wuhan University School of Chemistry and Chemical Engineering Anhui University Key Laboratory of Photochemical Conversion and Optoelectronic Materials & CAS-HKU Joint Laboratory on New Materials Technical Institute of Physics and Chemistry Chinese Academy of Sciences

Photoactive complexes of nonprecious transition metals, mainly including those in the first-row and partially the second-row of the Periodic table of elements, have received increasing attention in view of their low cost and long-term sustainability. They are recognized as promising alternatives to noble transition metal complex congeners that have been extensively studied in optoelectronic devices, artificial photosynthesis, photocatalysis, biodiagnostics, and therapeutics, etc. This review is devoted to a comprehensive summary on the classical and recent advances on photoactive nonprecious transition metal complexes, including photoactive Zr, V, Cr, Mo, and W complexes, Mn complexes and hybrids, Fe, Co, Ni, and Cu complexes, and Zn and Cd complexes and hybrids. A particular focus is given on the molecular design, modulation of photophysical and photochemical properties, and applications of the representative and lately-developed nonprecious metal complexes. In addition, a perspective on the future development in this field is provided at the end of this review.

关键词： photoactive metal complexes earth-abundant metals nonprecious metal complexes luminescence photochemistry

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Enhanced subacute ischemic stroke treatment via multistage drug delivery system design for combination therapy

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Science China Chemistry 2025年

作者： Xihan xu Juan-Juan li Jiamin li Hui-yan Zhang Yilin Qi Bing-Sen Shi Tianqi li Shan-Shan Su Weitao zhao Chang Chen Zhongying Gong Huiyu liu Ke-Rang Wang Dong-Sheng Guo xue xue State Key Laboratory of Medicinal Chemical Biology College of Pharmacy Nankai University College of Chemistry State Key Laboratory of Elemento-Organic Chemistry Key Laboratory of Functional Polymer Materials (Ministry of Education) Frontiers Science Center for New Organic Matter Collaborative Innovation Center of Chemical Science and EngineeringNankai University College of Chemistry and Materials Science State Key Laboratory of New Pharmaceutical Preparations and Excipients Key Laboratory of Medicinal Chemistry and Molecular Diagnosis (Ministry of Education) Key Laboratory of Chemical Biology of Hebei Province Hebei Research Center of the Basic Discipline of Synthetic Chemistry Hebei University Department of Neurology Tianjin First Central Hospital School of Medicine Nankai University Beijing Advanced Innovation Center for Soft Matter Science and Engineering State Key Laboratory of Organic-Inorganic Composites Beijing Laboratory of Biomedical Materials Bionanomaterials & Translational Engineering Laboratory Beijing Key Laboratory of BioprocessBeijing University of Chemical Technology

The management of acute ischemic stroke remains challenging due to its abrupt onset and the narrow treatment window,resulting in high rates of disability and *** therapy for neuroprotection and neurorepair strategies provided new hopes for subacute stroke *** we designed a multistage nanodelivery system that matches the unique microenvironment of the subacute phase of stroke,which utilizes calixarene as a building block to effectively load the neuroprotective drug *** calixarene is designed with glycosidic linkages targeting the glucose transporter 1 (GLUT1)on the blood-brain barrier (BBB) and azo bonds that are responsive to hypoxic ***,the decomposed calixarene molecules themselves possess intrinsic bio-activation to modulate inflammatory responses,achieving a multistage therapeutic approach that transitions from targeting to release and finally to *** approach combines anti-inflammation and neuroprotection,providing a multi-level treatment *** system was validated in a rat model of permanent middle cerebral artery occlusion (pMCAO),demonstrating that intravenous administration of the nanodelivery system repairs brain damage and improves motor function 24 h post-pMCAO *** work offers a precise and integrative strategy for stroke patients missing the acute therapy window to improve functional recovery and enhance the effect of comprehensive treatment.

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Einstein Probe discovery of EP240408a: A peculiar X-ray transient with an intermediate timescale

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 222-240页

作者： Wenda Zhang Weimin Yuan Zhixing ling yong Chen Nanda Rea Arne Rau Zhiming Cai Huaqing Cheng Francesco Coti Zelati lixin Dai Jingwei Hu Shumei Jia Chichuan Jin Dongyue li Paul O’Brien Rongfeng Shen Xinwen Shu Shengli Sun Xiaojin Sun Xiaofeng Wang Lei yang Bing Zhang Chen Zhang Shuang-Nan Zhang yonghe Zhang jie An David Buckley Alexis Coleiro Bertrand Cordier liming Dou Rob Eyles-Ferris Zhou Fan Hua Feng Shaoyu Fu Johan P.U.Fynbo Lluis Galbany Saurabh W.Jha Shuaiqing Jiang Albert Kong Erik Kuulkers Weihua Lei Wenxiong li Bifang liu Mingjun liu Xing liu Yuan liu Zhu liu Chandreyee Maitra Alessio Marino Itumeleng Monageng Kirpal Nandra Jeremy Sanders Roberto Soria lian Tao Junfeng Wang Song Wang Tinggui Wang Zhongxiang Wang Qingwen Wu xuefeng Wu Dong xu yanjun xu Suijian xue yongquan xue Zijian Zhang Zipei Zhu Hu Zou Congying Bao Fansheng Chen Houlei Chen Tianxiang Chen Wei Chen Yehai Chen Yifan Chen Chenzhou Cui Weiwei Cui yanfeng Dai Dongwei Fan Ju Guan Dawei Han Dongjie Hou Haibo Hu Maohai Huang Jia Huo Zhenqing Jia Bowen Jiang Ge Jin Chengkui li Junfei li Longhui li Maoshun li Wei li Zhengda li Tianying lian Congzhan liu Heyang liu Huaqiu liu Fangjun Lu Laidan Luo Jia Ma xuan Mao Haiwu Pan Xin Pan liming Song Hui Sun Yunyin Tan Qingjun Tang Yihan Tao Hao Wang Juan Wang Lei Wang Wenxin Wang Yilong Wang Yusa Wang Qinyu Wu Haitao xu Jingjing xu Xinpeng xu Yunfei xu zhao xu Changbin xue Yulong xue Ailiang yan Haonan yang Xiongtao yang yanji yang Juan Zhang Mo Zhang Wenjie Zhang Zhen Zhang Zhen Zhang Ziliang Zhang Donghua zhao Haisheng zhao Xiaofan zhao Zijian zhao hongyan Zhou Yilin Zhou Yuxuan Zhu Zhencai Zhu National Astronomical Observatories Chinese Academy of Sciences School of Astronomy and Space Sciences University of Chinese Academy of Sciences Key Laboratory of Particle Astrophysics Institute of High Energy Physics Chinese Academy of Sciences Institute of Space Sciences (ICE) CSIC Institut d’Estudis Espacials de Catalunya (IEEC) Max Planck Institute for Extraterrestrial Physics Innovation Academy for Microsatellites Chinese Academy of Sciences Department of Physics University of hong Kong School of Physics and Astronomy University of Leicester School of Physics and Astronomy Sun Yat-sen University Department of Physics Anhui Normal University Shanghai Institute of Technical Physics Chinese Academy of Sciences Physics Department Tsinghua University Nevada Center for Astrophysics University of Nevada Las Vegas Department of Physics and Astronomy University of Nevada Las Vegas South African Astronomical Observatory Department of Astronomy University of Cape Town Department of Physics University of the Free State Université Paris Cité CNRS Astroparticule et Cosmologie Université Paris Cité CEA Paris-Saclay IRFU/DAp-AIM Department of Astronomy Guangzhou University Cosmic Dawn Center (DAWN) Niels Bohr Institute University of Copenhagen Department of Physics and Astronomy Rutgers the State University of New Jersey Institute for Cosmic Ray Research The University of Tokyo ESA/ESTEC Department of Astronomy School of Physics Huazhong University of Science and Technology INAF-Osservatorio Astrofisico di Torino Sydney Institute for Astronomy School of Physics The University of Sydney Department of Astronomy Xiamen University Institute for Frontiers in Astronomy and Astrophysics Beijing Normal University Department of Astronomy University of Science and Technology School of Astronomy and Space Sciences University of Science and Technology of China Institute of Deep Space Sciences Deep Space Exploration Laboratory Department of Astronomy School of Physics and Astronomy Yunnan University Shanghai Astronomical Observatory Chi

We report the discovery of a peculiar X-ray transient,EP240408a,by Einstein Probe （EP） and follow-up studies made with EP,Swift,NICER,GROND,ATCA and other ground-based multiwavelength *** new transient was first detected with Wide-field X-ray Telescope （WXT） on board EP on April 8th,2024,manifested in an intense yet brief X-ray flare lasting for *** flare reached a peak flux of 3.9×10-9erg cm-2s-1in 0.5-4 ke V,～300 times brighter than the underlying X-ray emission detected throughout the *** and more precise follow-up observations by EP/FXT,Swift and NICER confirmed the finding of this new *** X-ray spectrum is non-thermal in 0.5-10 ke V,with a power-law photon index varying *** X-ray light curve shows a plateau lasting for ～4 d,followed by a steep decay till becoming undetectable ～10 d after the initial *** on its temporal property and constraints from previous EP observations,an unusual timescale in the range of 7-23 d is found for EP240408a,which is intermediate between the commonly found fast and long-term *** counterparts have been found in optical and near-infrared,with the earliest observation at 17 h after the initial X-ray detection,suggestive of intrinsically weak emission in these *** demonstrate that the remarkable properties of EP240408a are inconsistent with any of the transient types known so far,by comparison with,in particular,jetted tidal disruption events,gamma-ray bursts,X-ray binaries and fast blue optical *** nature of EP240408a thus remains an *** suggest that EP240408a may represent a new type of transients with intermediate timescales of the order of～10 *** detection and follow-ups of more of such objects are essential for revealing their origin.

关键词： X-ray transients ${\bm~{Einstein~~Probe}}$

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Epigenetics in the modern era of crop improvements

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Science China life Sciences 2025年

作者： yan xue Xiaofeng Cao Xiangsong Chen Xian Deng Xingwang Deng yong Ding Aiwu Dong Chengguo Duan Xiaofeng Fang Lei Gong Zhizhong Gong Xiaofeng Gu Chongsheng He Hang He Shengbo He Xinjian He yan He Yuehui He Guifang Jia Danhua Jiang Jianjun Jiang Jinsheng Lai zhaobo Lang Chenlong li Qing li Xingwang li Bao liu Bing liu Xiao Luo Yijun Qi Weiqiang Qian Guodong Ren Qingxin Song Xianwei Song Zhixi Tian Jiawei Wang Yuan Wang liang Wu Zhe Wu Rui Xia Jun Xiao lin xu Zhengyi xu Wenhao yan hongchun yang Jixian Zhai Yijing Zhang Yusheng zhao xuehua Zhong Daoxiu Zhou Ming Zhou Yue Zhou Bo Zhu Jiankang Zhu Qikun liu College of life Sciences Institute of Life Science and Green Development Hebei University Biotechnology Research Institute Chinese Academy of Agricultural Sciences College of Biology Hunan Key Laboratory of Plant Functional Genomics and Developmental Regulation Hunan Engineering and Technology Research Center of Hybrid Rapeseed Hunan University Institute of Advanced Agricultural Sciences School of Advanced Agricultural Sciences Peking University State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Guangdong Laboratory for Lingnan Modern Agriculture South China Agricultural University National Institute of Biological Sciences Key Laboratory of Seed Innovation Institute of Genetics and Developmental Biology Chinese Academy of Sciences School of Advanced Agricultural Sciences Peking-Tsinghua Center for Life Sciences Peking University Synthetic and Functional Biomolecules Center Beijing National Laboratory for Molecular Sciences Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education College of Chemistry and Molecular Engineering Peking University Key Laboratory of Seed Innovation State Key Laboratory of Plant Genomics and National Center for Plant Gene Research Institute of Genetics and Developmental Biology Chinese Academy of Sciences State Key Laboratory of Wheat Improvement Peking University Institute of Advanced Agricultural Sciences Shandong Laboratory of Advanced Agricultural Sciences in Weifang State Key Laboratory of Crop Stress Adaptation and Improvement School of Life Sciences Henan University State Key Laboratory of Maize Bio-breeding National Maize Improvement Center Department of Plant Genetics and Breeding China Agricultural University Frontiers Science Center for Molecular Design Breeding China Agricultural University Center for Crop Functional Genomics and Molecular Breeding China Agricultural University Sanya Institute of China Agricultural University Hainan Yazhou Bay Seed Laboratory Institute of Advan

Epigenetic mechanisms are integral to plant growth, development, and adaptation to environmental stimuli. Over the past two decades,our comprehension of these complex regulatory processes has expanded remarkably, producing a substantial body of knowledge on both locus-specific mechanisms and genome-wide regulatory patterns. Studies initially grounded in the model plant Arabidopsis have been broadened to encompass a diverse array of crop species, revealing the multifaceted roles of epigenetics in physiological and agronomic traits. With recent technological advancements, epigenetic regulations at the single-cell level and at the large-scale population level are emerging as new focuses. This review offers an in-depth synthesis of the diverse epigenetic regulations, detailing the catalytic machinery and regulatory functions. It delves into the intricate interplay among various epigenetic elements and their collective influence on the modulation of crop traits. Furthermore, it examines recent breakthroughs in technologies for epigenetic modifications and their integration into strategies for crop improvement. The review underscores the transformative potential of epigenetic strategies in bolstering crop performance, advocating for the development of efficient tools to fully exploit the agricultural benefits of epigenetic insights.

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Modeling and simulation of muzzle flow field of railgun with metal vapor and arc

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Defence Technology（防务技术） 2020年第4期16卷 802-810页

作者： Yuan Gao yan-jie Ni zhao-xin Wang Ying-tao xu yong Jin Bao-ming li National Key Laboratory of Transient Physics Nanjing University of Science and TechnologyNanjing 210094JiangsuChina China Academy of Ordnance 100089BeijingChina

During the electromagnetic railgun launching process,there will be a complex flow field with high temperature in the muzzle area because of the high-speed friction,transition and seco ndary *** paper models the muzzle area of railgun when the projectile is far away from the muzzle,and the dynamic simulation of the flow field with secondary arc in the muzzle area is carried out based on the magneto hydrodynamic ***,a multi-component plasma transport model is used to analyze the muzzle arc plasma flow process of the mixed gas of Al vapor and the ***,the pressure boundary conditions are fitted by the dynamic mesh simulation *** current and voltage of the muzzle are obtained through the emission experiment of the railgun experimental *** load the current data into the simulation model and the voltage of experiments and simulations are compared,which proves the accuracy of the *** the plasma temperature and the composition of Al vapor in the muzzle flow process are analyzed in-depth.

关键词： Railgun Flow field Muzzle Plasma Simulation Al vapor

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Herbal medicines and nonalcoholic fatty liver disease

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World Journal of Gastroenterology 2016年第30期22卷 6890-6905页

作者： hong Yao Yu-jie Qiao Ya-li zhao xu-Feng Tao li-Na xu lian-hong Yin yan Qi Jin-yong Peng College of Pharmacy Dalian Medical University Dalian 116044 Liaoning Province China

Nonalcoholic fatty liver disease(NAFLD), which is characterized by excessive fat accumulation in the liver of patients who consume little or no alcohol, becomes increasingly common with rapid economic development. Long-term excess fat accumulation leads to NAFLD and represents a global health problem with no effective therapeutic approach. NAFLD is considered to be a series of complex, multifaceted pathological processes involving oxidative stress, inflammation, apoptosis, and metabolism. Over the past decades, herbal medicines have garnered growing attention as potential therapeutic agents to prevent and treat NAFLD, due to their high efficacy and low risk of side effects. In this review, we evaluate the use of herbal medicines(including traditional Chinese herbal formulas, crude extracts from medicinal plants, and pure natural products) to treat NAFLD. These herbal medicines are natural resources that can inform innovative drug research and the development of treatments for NAFLD in the future.

关键词： Herbal medicines Nonalcoholic fatty liver disease Natural product Traditional Chinese medicines Review

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Influence of metallurgical processing parameters on defects in cold-rolled steel sheet caused by inclusions

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International Journal of Minerals,Metallurgy and Materials 2019年第4期26卷 440-446页

作者： Rui Wang yan-ping Bao Yi-hong li Zhi-jie yan Da-zhao li yan Kang School of Materials Science and Engineering North University of China State Key Laboratory of Advanced Metallurgy University of Science and Technology Beijing School of Materials Science and Engineering Taiyuan University of Science and Technology

The cleanliness and defects for cold-rolled steel sheet caused by inclusions are greatly influenced by parameters in the metallurgical processing. Good control of parameters during the processing can lead to a better product. In this paper, data mining was used to explore the influence of parameters on defects in steel sheets. A decision tree model was established and it was found that the oxygen content before deoxidation, the end-point temperature of the converter, and the temperature before deoxidation had a great impact on the defects in the cold-rolled sheet that were caused by inclusions. This finding was confirmed by experiments with infrared absorption, scanning electron microscopy(SEM), energy dispersive X-ray spectroscopy(EDS), and automatic inclusion analysis methods. After optimization according to results from the model and experiments, the defect rate caused by the inclusions was reduced from 0.92% to 0.38%.

关键词： IF steel steel cleanliness data mining inclusions

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Microstructural evolution and change in hardness during creep of NF709 austenitic stainless steel

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Acta Metallurgica Sinica(English Letters) 2011年第3期24卷 220-224页

作者： yan zhao jie zhao Xiaona li School of Material Science and Engineering Dalian University of Technology Dalian 116085 China Key Laboratory of Materials Modification by Laser Ion and Electron Beams of Ministry of Education Dalian University of Technology Dalian 116085 China

Microstructural evolution and the change in hardness during creep deformation of NF709 austenitic stainless steel were investigated. Creep tests were carried out at 650 ℃ for 2932 h under a load of 210 MPa for comparison with aging specimen at 650 ℃ for 3000 h. The hardness results indicated that applied stress during creep process induced hardness increase. Analysis of longitudinal section microstructure showed that the creep damage caused by pores and the grain boundary hardening caused by elongated grains could be the factors leading to hardness differences. The C phase dispersedly precipitated in intragranular and interacted with dislocations during creep process, indicating strain hardening.

关键词： NFT09 Creep-introduced Hardness Microstructural evolution

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Impact of consolidation pressure on contaminant migration in clay liner

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Water Science and Engineering 2013年第3期6卷 340-353页

作者： Zhi-hong ZHANG zhao-gang xu Xiu-li DU hong-yan li The Key Laboratory of Urban Security and Disaster Engineering Ministry of Education Beijing University of Technology

Consolidation deformation occurs in clay liners under the self-weight of wastes at a simple garbage dump or dredged sediment dump, which leads to a decrease in the porosity. However. the migration of contaminants in clay liners is influenced by the porosity. Thus, the impact of consolidation deformation of clay liners on the migration of contaminants cannot be ignored. Based on Biot＇s consolidation theory, the contaminant migration theory, and consideration of the three kinds of migration mechanisms of convection, diffusion, and adsorption, a one-dimensional migration model of contaminants in deforming porous media was established, and the finite difference method was adopted to obtain the numerical solutions for an established initial-boundary value problem. The impact of consolidation pressure on the migration law of a contaminant was studied. The results show that, regardless of adsorption modes, different consolidation pressures have similar impacts on the migration law of the contaminant. Namely, over a certain migration time, the greater the consolidation pressure is, the smaller the migration depth of the contaminant. The results also show that, while the migration time increases, the impact of a certain increment of consolidation pressure on the variation of contaminant concentration with the depth increases gradually and, while the migration depth increases, the impact of a certain increment of consolidation pressure on the variation of the contaminant concentration with time increases gradually.

关键词： clay liner consolidation pressure consolidation deformation. contaminantmigration

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