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Frontiers of Chemical Science and Engineering 2021年第2期15卷 421-436页

作者： Jin Zhao Qing-Xi Wu Xiao-Du Cheng Ting Su Xiao-Hui Wang Wen-Na Zhang yong-ming lu Yan Chen School of Life Sciences Anhui UniversityHefei 230601China Anhui Key Laboratory of Modern Biomanufacturing Hefei 230601China

Fungi play an important role in dying wastewater *** this work,the mycelia of Lactarius deliciosus exhibited an excellent capacity in decolorizing coomassie brilliant blue(CBB).The results demonstrated that the mycelia could treat CBB with high concentrations over a broad range of pH and *** decolorization rate of 99.19%and the removal rate of 16.31 mg·L^(‒1)·h were *** mycelia could be recycled from decolorizing process for 19 times,indicating a good *** verified that the lignin peroxidase(121.65 U·L^(‒1))and manganese peroxidase(36.77 U·L^(‒1))were involved in the degradation and decolorization process of *** assessments indicated the seed germination rate was up to 82.22%while inhibition to Escherichia coli decreased dramatically and no significant effect on Caenorhabditis elegans growth was *** removal of CBB was a synergistic process accomplished by adsorption and *** mycelia could be used for eco-friendly CBB treatment.

关键词： fungus mycelia biodegradation extracellular enzymes coomassie brilliant blue Lactarius deliciosus

MLH1 promoter germline-methylation in selected probands of Chinese hereditary non-polyposis colorectal cancer families

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World Journal of Gastroenterology 2008年第48期14卷 7329-7334页

作者： Heng-Hua Zhou Shi-Yan Yan Xiao-Yan Zhou Xiang Du Tai-ming Zhang Xu Cai yong-ming lu San-Jun Cai Da-Ren Shi Department of Pathology Cancer Hospital/Institute Fudan University Department of Oncology Shanghai Medical College Fudan University Shanghai 209032 China Department of Gastroenterology Xinhua Hospital Shanghai Jiaotong University School of Medicine Shanghai 200092 China Department of Abdominal Surgery Cancer Hospital/Institute Fudan University Shanghai 200032 China

AIM： To detect the MLH1 gene promoter germline- methylation in probands of Chinese hereditary non- polyposis colorectal cancer （HNPCC）, and to evaluate the role of methylation in MLH1 gene promoter and molecular genetics in screening for ***： The promoter germline methylation of MLH1 gene was detected by methylation-specific PCR （MSP） in 18 probands from unrelated HNPCC families with high microsatellite-instability （MSI-H） phenotype but without germline mutations in MSH2, MLH1 and MSH6 genes. At the same time, 6 kindreds were col- lected with microsatellite-stability （MSS） phenotype but without germline mutations in MSH2, MIH1 and MSH6 genes as controls. The results of MSP were confirmed by clone sequencing. To ensure the reliability of the results, family H65 with nonsense germline mutation at c.2228C 〉 A in MSH2 gene was used as the negative control and the cell line sw48 was used as the known positive control along with water as the blank control. Immunochemical staining of MIH1 protein was performed with Envision two-step method in those patients with aberrant methylation to judge whether the status of MLH1 gene methylation affects the expression of MLH1 ***： Five probands with MIH1 gene promoter methylation were detected in 18 Chinese HNPCC families with MSI-H phenotype but without germline mutations in MSH2, MLH1 and MSH6 genes. Two of the five probands from families H10 and H29 displayed exhaustive-methylation, fulfilling the Japanese criteria （JC） and the Amsterdam criteria （AC）, respectively. The other 3 probands presented part-methylation fulfilling the AC. Of the 13 probands with unmethylation phenotype, 8 fulfilled the JC and the Bethesda guidelines （BG）, 5 fulfilled the AC. The rate of aberrant methylation in MLH1 gene in the AC group （22.2%, 4/18） was higher than that in the JC/BG groups （5.6%, 1/18） in all HNPCC families with MSI-H phenotype but without germline mutations in PISH2, PIIH1 and MSH6 genes. However, no proband with m

关键词： Hereditary non-polyposis colorectal cancer MLH1 Methylation Germline Methylation-specific PCR Microsatellite phenotype

Clinicopathologic significance of HER-2/neu protein expression and gene amplification in gastric carcinoma

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World Journal of Gastroenterology 2011年第11期17卷 1501-1506页

作者： Shi-Yan Yan Ying Hu Jian-Gao Fan Guo-Quan Tao yong-ming lu Xu Cai Bao-Hua Yu Yi-Qun Du Department of Gastroenterology Xinhua Hospital Shanghai Jiaotong University School of Medicine Shanghai 200092 China Department of General Surgery Huaian No.1 People's Hospital affiliated to Nanjing Medical University Huaian 223300 Jiangsu Province China Department of Pathology Cancer Hospital Fudan University Shanghai 200032 China Department of Pathology Institute of Traditional Chinese Medicine Nanjing 210029 Jiangsu Province China

AIM:To study the HER-2/neu protein expression and gene amplification in gastric carcinoma and their ***:One hundred and forty-five formalin-fixed and paraffin-embedded tumor tissue samples from Chinese gastric carcinoma patients were studied with immunohistochemistry(IHC)and fluorescence in situ hybridization(FISH)*** data about all patients were ***:The levels of HER-2 3+,HER-2 2+and HER21+were measurable in 6.9%,8.3%and 17.2%of the samples,*** HER-2 was stained in 67.6% of the *** showed that HER-2 gene was amplified in 18 samples,10 HER-2 3+samples,5 HER-2 2+samples,and 3 HER-2 1+samples with IHC ***-2 status was not correlated with the sex and age of patients,and tumor size,location or differentiation,but with the depth of invasion,TNM stage,lymph node and distant metastasis as well as histopathological classification of gastric cancer(PluSION:All samples with IHC as HER-2 expression should be analyzed with *** of HER-2 gene amplification can assess the malignant biological behaviors and prognosis of gastric cancer.

关键词： Gastric carcinoma HER-2 Clinocopathologic significance Immunohistochemistry Fluorescence in situ hybridization

Three novel missense germline mutations in different exons of MSH6 gene in Chinese hereditary non-polyposis colorectal cancer families

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World Journal of Gastroenterology 2007年第37期13卷 5021-5024页

作者： Shi-Yan Yan Xiao-Yan Zhou Xiang Du Tai-ming Zhang yong-ming lu San-Jun Cai Xiao-Li Xu Bao-Hua Yu Heng-Hua Zhou Da-Ren Shi Department of Pathology Cancer Hospital/InstituteFudan University Department of Oncology Shanghai MedicalCollege Fudan University Shanghai 200032 China Department of Abdominal Surgery CancerHospital/Institute Fudan University Shanghai 200032 China

AIM: To investigate the germline mutations of MSH6 gene in probands of Chinese hereditary non-polyposis colorectal cancer (HNPCC) families fulfilling different clinical criteria. METHODS: Germline mutations of MSH6 gene were detected by PCR-based DNA sequencing in 39 unrelated HNPCC probands fulfilling different clinical criteria in which MSH2 and MLH1 mutations were excluded. To further investigate the pathological effects of detected missense mutations, we analyzed the above related MSH6 exons using PCR-based sequencing in 137 healthy persons with no family history. The clinicopathological features were collected from the Archive Library of Cancer Hospital, Fudan University and analyzed. RESULTS: Four germline missense mutations distributed in the 4th, 6th and 9th exons were observed. Of them, three were not found in international HNPCC databases and did not occur in 137 healthy controls, indicating that they were novel missense mutations. The remaining mutation which is consistent with the case H14 at c.3488A>T of exon 6 of MSH6 gene was also found in the controls, the rate was approximately 3.65% (5/137) and the type of mutation was not found in the international HNPCC mutational and SNP databases, suggesting that this missense mutation was a new SNP unreported up to date. CONCluSION: Three novel missense mutations and a new SNP observed in the probands of Chinese HNPCC families, may play an important role in the development of HNPCC.

关键词： Hereditary non-polyposis colorectal cancer MSH6 Missense mutation Colorectal cancer

Improving a Hybrid Method of Indoor Propagation Modeling for Wireless Communications Systems

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The Journal of China Universities of Posts and Telecommunications 2003年第3期10卷 41-45页

作者： HUANG yong ming, lu Ying hua, XU Li, FENG Xiao jun, ZHANG Hong xin (Continuing Education School, Beijing University of Posts and Telecommunications, Beijing 100876, P.R.China) Continuing Education School Beijing University of Posts and Telecommunications Beijing 100876 P.R.China

Propagation prediction is very important in the design of wirelesscommunication systems. A combined ray tracing and Finite-Difference Time-Domain (FDTD) method isimproved on modeling the indoor radio propagation by applying Perfectly Matched Layer (PML)Absorbing Boundary Conditions (ABCs) to FDTD grid. Thus, more accurate propagation prediction can beobtained.

关键词： ray tracing FDTD hybrid method wireless communications systems indoorradio propagation

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1.2m微晶主镜的新型支撑

光学精密工程 2016年第10期24卷 2462-2470页

作者：邵亮赵勇志明名吕天宇刘昌华王洪浩中国科学院长春光学精密机械与物理研究所吉林长春130033

针对1.2m微晶主镜,提出了基于6套柔性切向杆机构的侧向支撑与基于18点半柔性Whiffletree机构的轴向支撑相结合的新型主镜支撑方案,用于保证该主镜在较大温差范围以及不同俯仰角度下始终保持良好的面形精度及较高的系统刚度。分析了该机... 详细信息

针对1.2m微晶主镜,提出了基于6套柔性切向杆机构的侧向支撑与基于18点半柔性Whiffletree机构的轴向支撑相结合的新型主镜支撑方案,用于保证该主镜在较大温差范围以及不同俯仰角度下始终保持良好的面形精度及较高的系统刚度。分析了该机构的工作原理,实验测试了主镜的面形精度及支撑系统的模态。机构分析表明该支撑方式可有效保证主镜定位精度和面形精度,并具有热解耦能力;有限元分析确认系统具有良好的支撑性能;面形精度检测得出主镜光轴垂直面形精度RMS达15.25nm,光轴水平面形精度RMS为20.75nm,模态测试则获得主镜支撑系统的一阶固有频率为60.3Hz。实测结果验证了该新型主镜支撑系统具有良好的面形保持能力及支撑刚度,分析结果与实测结果符合度较好,主镜光轴垂直和水平状态面形精度RMS的相对误差分别为14.0%和17.8%,一阶固有频率相对误差为10.8%。得到的结果验证了有限元建模及分析的可信性,支撑系统设计方案的合理性及相关理论推导的正确性。

关键词：微晶主镜主镜支撑面形检测模态测试有限元分析

The high levels of DLC-1 contribute to the carcinogenesis in human gliomas

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The high levels of DLC-1 contribute to the carcinogenesis in...

An extremely painful leg ulcer:a misdiagnosed case report with literature review

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： yong-Jian lu,ming-Chang LI,Zhong-Min YUAN~* Institute of Neuroscience and the Second Affiliated Hospital,Guangzhou Medical College,Guangzhou,510260,China

＜正＞The decrease of DLC-1（deleted in liver cancer -1） expression led to an aberrant increase in the GTPase activity of Rho factors which contributes to the *** evidences suggested that DLC-1 acted as a tumor suppressor in a number of common human cancers,including HCC,breast,colon,ovarian,uterine,gastric,lung,pancreatic, prostate,renal and nasopharyngeal ***,the role of DLC-1 in human gliomas remained *** the study, we found that human glioma tissues presented high levels of DLC-1 compared with the control tissues by real-time PCR（P ＜0.001）.Western blot and Immunohistochemistry confirmed the results ***,the DLC-1 mRNA levels in WHO grade I gliomas were considerably higher than that in gradeⅡ-Ⅳgliomas（P＜0.001）.By the utility of the glioma cell lines U25 IMG and U87MG,we determined that the mRNA levels of DLC-1 did not change in the condition of 5-aza-2’- deoxycytidine（5’-AZA） alone or the combination of 5’-AZA and trichostatin A（TSA）.Overexpression of wild type DLC- 1 significantly promoted the proliferation of the glioma cells,while DLC- 1/K714E,a dominant negative mutant without GAP enzyme activity,inhibited the ***,our data defined a specific role for DLC-1 that in human gliomas the high levels of DLC-1 may contribute to the carcinogenesis.

关键词： DLC-1 glioma GAP

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An extremely painful leg ulcer:a misdiagnosed case report wi...

线粒体DNA拷贝数:一种新型的职业与环境暴露生物标志

2007年浙江省周围血管外科年会

作者： yong-ming lu,MD Wen-Jun Zhao,MD yong-Hua Zheng,MD Bo-Guang Fan,MD,Ph.D Department of Surgery,Wenzhou Medical University Taizhou Hospital of Zhejiang Province,China.

A man was admitted to hospital with leg ulcers,which was multiple in left malleolus, tibia,dorsal aspect of the feet and *** patient was initially treated with wound dressings,antibiotics and other surgical debridement,but all those treatments were *** results from laboratory and image examinations showed no abnormal,and did not support the diagnosis of vascular and specific infection diseases. Subsequently,the patient confessed that he has been on medication with hydroxyurea for his granulocytic *** leg ulcers were fully cured after discontinuation of h *** case confirms the relationship between leg ulcers and hydroxyurea administration,and therefore,we suggest that it should always consider the other reason for chronic ulcer in patient is a medication.

关键词： chronic granulocytic leukemia hydroxyurea leg ulcer

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环境与职业医学 2017年第6期34卷 560-565,570页

作者：王铁英李静张叶寿勇明李芳陆荣柱高静江苏大学医学院预防医学与卫生检验系江苏镇江212013 江苏大学药学院药理学系江苏镇江212013 上海化学工业园区医疗中心上海201507

线粒体是职业和环境化学物毒效应的重要靶细胞器,线粒体相应结构和功能指标可能成为化学物暴露的早期效应标志。本文综述了外周组织线粒体DNA拷贝数(mtDNAcn)在职业和环境化学物暴露下的变化特征,苯、二甲基甲酰胺等有机溶剂及多环芳烃... 详细信息

线粒体是职业和环境化学物毒效应的重要靶细胞器,线粒体相应结构和功能指标可能成为化学物暴露的早期效应标志。本文综述了外周组织线粒体DNA拷贝数(mtDNAcn)在职业和环境化学物暴露下的变化特征,苯、二甲基甲酰胺等有机溶剂及多环芳烃暴露可增加mtDNAcn,但空气颗粒物与烟草烟雾的长期暴露却又能降低mtDNAcn,因而表明mtDNAcn在不同毒物及其暴露时间和剂量下可能会出现不同的效应特征,mtDNAcn在职业和环境化学物早期危害效应监护中的作用值得研究。

关键词：线粒体 DNA拷贝数化学物暴露生物标志

Construction technology for deep tunnels crossing superhigh-temperature fault zones with high water surges

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Frontiers of Structural and Civil Engineering 2024年第8期18卷 1267-1280页

作者： yong ZHAO Tingyu ZHU Li YU ming lu Sichuan–Xizang Railway Co. Ltd.Linzhi 860000China School of Civil Engineering Southwest Jiaotong UniversityChengdu 610036China

The harsh environment in tunnels with high geothermal temperatures and humidity can adversely impact machinery,personnel,and *** main causes of specific problems are the unknown mechanisms of local geothermal formation,inappropriate temperature control measures,and insufficient systematic *** this study,three work sections relating to a high geothermal tunnel are:the tunnel face,middle-of-tunnel section,and outside-oftunnel section.A cooling strategy is proposed to offer technical support in achieving comprehensive cooling,overall as well as for each of the ***,a comprehensive geological survey explores the mechanism and exact location of the heat ***,grouting and centralized drainage measures are used to control the heat release of hot *** ventilation,ice chillers and other applicable measures are used to control the ambient ***,a monitoring and early warning system is established to prevent *** cooling strategy has been applied in the field with good results.

关键词： high geothermal tunnels cooling strategy comprehensive cooling measures safety and security