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Single-cell sequencing technology in diabetic wound healing:New insights into the progenitors-based repair strategies

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World Journal of Stem Cells 2024年第5期16卷 462-466页

作者： Zhen Xiang Rui-Peng Cai Yang Xiao yong-can huang Shenzhen Engineering Laboratory of Orthopaedic Regenerative Technologies Department of Spine SurgeryPeking University Shenzhen HospitalShenzhen 518036Guangdong ProvinceChina Department of Breast and Thyroid Surgery Peking University Shenzhen HospitalShenzhen 518036Guangdong ProvinceChina

Diabetes mellitus(DM),an increasingly prevalent chronic metabolic disease,is characterised by prolonged hyperglycaemia,which leads to long-term health *** much effort has been put into understanding the pathogenesis of diabetic wounds,the underlying mechanisms remain *** advent of single-cell RNA sequencing(scRNAseq)has revolutionised biological research by enabling the identification of novel cell types,the discovery of cellular markers,the analysis of gene expression patterns and the prediction of develop-mental *** powerful tool allows for an in-depth exploration of pathogenesis at the cellular and molecular *** this editorial,we focus on progenitor-based repair strategies for diabetic wound healing as revealed by scRNAseq and highlight the biological behaviour of various healing-related cells and the alteration of signalling pathways in the process of diabetic wound *** could not only deepen our understanding of the complex biology of diabetic wounds but also identify and validate new targets for inter-vention,offering hope for improved patient outcomes in the management of this challenging complication of DM.

关键词： Single-cell sequencing Diabetic wound healing Cell subpopulations Heterogeneity Pathogenesis Progenitor cells

Evaluation of genetic response of mesenchymal stem cells to nanosecond pulsed electric fields by whole transcriptome sequencing

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World Journal of Stem Cells 2024年第3期16卷 305-323页

作者： Jian-Jing Lin Tong Ning Shi-Cheng Jia Ke-Jia Li yong-can huang Qiang Liu Jian-Hao Lin Xin-Tao Zhang Department of Sports Medicine and Rehabilitation Peking University Shenzhen HospitalShenzhen 518036Guangdong ProvinceChina Institute of Medical Science The Second HospitalCheeloo College of MedicineShandong UniversityJinan 250033Shandong ProvinceChina Department of Biomedical Engineering Institute of Future TechnologyPeking UniversityBeijing 100871China Shenzhen Engineering Laboratory of Orthopaedic Regenerative Technologies Peking University Shenzhen HospitalShenzhen 518036Guangdong ProvinceChina Arthritis Clinical and Research Center Peking University People’s HospitalBeijing 100044China

BACKGROUND Mesenchymal stem cells(MSCs)modulated by various exogenous signals have been applied extensively in regenerative medicine ***,nanosecond pulsed electric fields(nsPEFs),characterized by short duration and high strength,significantly influence cell phenotypes and regulate MSCs differentiation via multiple ***,we used transcriptomics to study changes in messenger RNA(mRNA),long noncoding RNA(lncRNA),microRNA(miRNA),and circular RNA expression during nsPEFs *** To explore gene expression profiles and potential transcriptional regulatory mechanisms in MSCs pretreated with *** The impact of nsPEFs on the MSCs transcriptome was investigated through whole transcriptome *** were pretreated with 5-pulse nsPEFs(100 ns at 10 kV/cm,1 Hz),followed by total RNA *** transcript was normalized by fragments per kilobase per *** change and difference significance were applied to screen the differentially expressed genes(DEGs).Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed to elucidate gene functions,complemented by quantitative polymerase chain reaction *** In total,263 DEGs were discovered,with 92 upregulated and 171 *** were predominantly enriched in epithelial cell proliferation,osteoblast differentiation,mesenchymal cell differentiation,nuclear division,and wound *** cellular components,DEGs are primarily involved in condensed chromosome,chromosomal region,actin cytoskeleton,and *** aspect of molecular functions,DEGs are mainly involved in glycosaminoglycan binding,integrin binding,nuclear steroid receptor activity,cytoskeletal motor activity,and steroid *** real-time polymerase chain reaction confirmed targeted transcript *** Our systematic investigation of the wide-ranging transcriptional pattern modulated by nsPEFs revealed the differential expression of 26

关键词： Nanosecond pulsed electric fields Whole transcriptome sequencing Mesenchymal stem cells Genetic response Stem cell engineering

Identification of injury type using somatosensory and motor evoked potentials in a rat spinal cord injury model

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Neural Regeneration Research 2023年第2期18卷 422-427页

作者： Rong Li Han-Lei Li Hong-Yan Cui yong-can huang yong Hu Department of Orthopedics and Traumatology The University of Hong Kong-Shenzhen HospitalShenzhenGuangdong ProvinveChina Department of Neurosurgery Neuroscience CenterIntegrated Hospital of Traditional Chinese MedicineSouthern Medical UniversityGuangzhouGuangdong ProvinveChina Institute of Biomedical Engineering Chinese Academy of Medical Sciences and Peking Union Medical CollegeTianjinChina Shenzhen Engineering Laboratory of Orthopedic Regenerative Technologies Department of Spine SurgeryPeking University Shenzhen HospitalShenzhenGuangdong ProvinveChina Department of Orthopedics and Traumatology The University of Hong KongPokfulamHong Kong Special Administrative RegionChina

The spinal cord is at risk of injury during spinal *** intraoperative spinal co rd injury is identified early,irreve rsible impairment or loss of neurological function can be *** types of spinal cord injury result in damage to diffe rent spinal cord regions,which may cause diffe rent somatosensory and motor evoked potential signal res *** this study,we examined electrophysiological and histopathological changes between contusion,distra ction,and dislocation spinal cord injuries in a rat *** found that contusion led to the most severe dorsal white matter injury and caused considerable attenuation of both somatosensory and motor evoked *** resulted in loss of myelinated axons in the lateral region of the injured spinal cord along the rostrocaudal *** amplitude of attenuation in motor evoked potential responses caused by dislocation was greater than that caused by *** distraction injury,extracellular spaces were slightly but not significantly enlarged;somatosensory evoked potential res ponses slightly decreased and motor evoked potential responses were *** analysis showed that histological and electrophysiological findings we re significantly correlated and related to injury *** rative monitoring of both somatosensory and motor evoked potentials has the potential to identify iatrogenic spinal cord injury type during surgery.

关键词： contusion injury dislocation injury distraction injury electropnysiology heterogeneity histopathology injury mechanism motor evoked potential somatosensory evoked potential spinal cord injury

Tumor necrosis factor-αinhibition restores matrix formation by human adipose-derived stem cells in the late stage of chondrogenic differentiation

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World Journal of Stem Cells 2022年第11期14卷 798-814页

作者： Jiang-Tao Wan Xian-Shuai Qiu Zhuo-Hang Fu yong-can huang Shao-Xiong Min Department of Spine Surgery Peking University Shenzhen HospitalShenzhen 518036Guangdong ProvinceChina Institute of Orthopedics Shenzhen Peking University-The Hong Kong University of Science and Technology Medical CenterShenzhen 518036Guangdong ProvinceChina Department of Spine Surgery Zhujiang HospitalSouthern Medical UniversityGuangzhou 510280Guangdong ProvinceChina

BACKGROUND Cartilage tissue engineering is a promising strategy for treating cartilage *** formation by adipose-derived stem cells(ADSCs),which are one type of seed cell used for cartilage tissue engineering,decreases in the late stage of induced chondrogenic differentiation in vitro,which seriously limits research on ADSCs and their *** To improve the chondrogenic differentiation efficiency of ADSCs in vitro,and optimize the existing chondrogenic induction *** Tumor necrosis factor-alpha(TNF-α)inhibitor was added to chondrogenic culture medium,and then Western blotting,enzyme linked immunosorbent assay,immunofluorescence and toluidine blue staining were used to detect the cartilage matrix secretion and the expression of key proteins of nuclear factor kappa-B(NF-κB)signaling *** In this study,we found that the levels of TNF-αand matrix metalloproteinase 3 were increased during the chondrogenic differentiation of ***-αthen bound to its receptor and activated the NF-κB pathway,leading to a decrease in cartilage matrix synthesis and *** TNF-αwith its inhibitors etanercept(1μg/mL)or infliximab(10μg/mL)significantly restored matrix *** Therefore,this study developed a combination of ADSC therapy and targeted anti-inflammatory drugs to optimize the chondrogenesis of ADSCs,and this approach could be very beneficial for translating ADSC-based approaches to treat cartilage damage.

关键词： Adipose-derived mesenchymal stem cells Human adipose-derived mesenchymal stem cells Chondrogenic differentiation Tumor necrosis factor-alpha Etanercept Infliximab Nuclear factor kappa-B

Amelioration of ligamentum flavum hypertrophy using umbilical cord mesenchymal stromal cell-derived extracellular vesicles

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Bioactive Materials 2023年第1期19卷 139-154页

作者： Cheng Ma Xin Qi Yi-Fan Wei Zhi Li He-Long Zhang He Li Feng-Lei Yu Ya-Nan Pu yong-can huang yong-Xin Ren Department of Orthopaedics The First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu210029China Department of Pathogen Biology and Immunology Nanjing Medical UniversityNanjingJiangsu210029China Department of Orthopaedics Geriatric Hospital of Nanjing Medical UniversityNanjingJiangsu210024China Outpatient&Emergency Management Department The First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu 210029China Shenzhen Engineering Laboratory of Orthopaedic Regenerative Technologies Department of Spine SurgeryPeking University Shenzhen HospitalShenzhen518036China

Ligamentum flavum(LF)hypertrophy(LFH)has been recognised as one of the key contributors to lumbar spinal ***,no effective methods are available to ameliorate this *** this study,human umbilical cord mesenchymal stromal cell-derived extracellular vesicles(hUCMSC-EVs)were introduced for the first time as promising vehicles for drug delivery to treat *** downregulation of miR-146a-5p and miR-221-3p expressions in human LF tissues negatively correlated with increased LF *** hUCMSC-EVs enriched with these two miRNAs significantly suppressed LFH in vivo and notably ameliorated the progression of transforming growth factorβ1(TGF-β1)-induced fibrosis in vitro after delivering these two miRNAs to mouse LF *** results further demonstrated that miR-146a-5p and miR-221-3p directly bonded to the 3′-UTR regions of SMAD4 mRNA,thereby inhibiting the TGF-β/SMAD4 signalling ***,this translational study determined the effectiveness of a hUCMSC-EVs-based approach for the treatment of LFH and revealed the critical target of miR-146a-5p and *** findings provide new insights into promising therapeutics using a hUCMSC-EVs-based delivery system for patients with lumbar spinal stenosis.

关键词： Ligamentum flavum hypertrophy Fibrosis Umbilical cord mesenchymal stromal cells Extracellular vesicle miR-146a-5p miR-221-3p

Highly Improved Creep Resistance in Polypropylene Through Thermally Reduced Graphene Oxide and Its Creep Lifetime Prediction

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Chinese Journal of Polymer Science 2024年第2期42卷 256-266,I0010页

作者： can-can Zhang Jun-Long Yang Ya-Jiang huang Guang-Xian Li College of Polymer Science and Engineering State Key Laboratory of Polymer Materials Engineering of ChinaSichuan UniversityChengdu610065China

Polypropylene(PP) exhibits suboptimal creep resistance due to the presence of methyl groups on its main chain, leading to irregular chain segment distribution, diminished inter-chain interaction, and crystallinity. This structural feature causes chain slippage in PP under stress,significantly constraining its service lifetime. In this study, thermally reduced graphene oxide(TrGO) nanosheets were incorporated into the PP matrix, yielding a nanocomposite with exceptional creep resistance performance. Results demonstrated that at a stress of 25 MPa, a 2.0 wt% TrGO content could enhance the creep failure lifetime of PP by 21.5 times compared to neat PP. Rheology, transmission electron microscopy(TEM),and scanning electron microscopy(SEM) characterization techniques were employed to analyze the mechanism of TrGO's influence on PP's creep behavior. It was observed that when TrGO content exceeded 1.0 wt%, an effective particle network structure formed within the PP matrix. This homogeneously dispersed TrGO-formed particle network structure restricted the migration and rearrangement of PP molecular chains, enabling prolonged stress resistance without structural failure. By combining the time-strain superposition method with the critical failure strain as a criterion, generalized creep compliance curves for PP and its composites were established, facilitating the prediction of material creep failure lifetimes, with a strong agreement between experimental and predicted lifetime values. This research proposes a novel strategy aimed at developing polypropylene materials and products with enhanced long-term stability and durability, thus extending service life, reducing failure risk, and broadening their potential across various application domains.

关键词： Thermally reduced graphene oxide Polypropylene Creep failure and lifetime prediction

Mechanotransduction of stem cells for tendon repair

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World Journal of Stem Cells 2020年第9期12卷 952-965页

作者： Hao-Nan Wang yong-can huang Guo-Xin Ni School of Sport Medicine and Rehabilitation Beijing Sport UniversityBeijing 100084China Shenzhen Engineering Laboratory of Orthopaedic Regenerative Technologies Department of Spine SurgeryPeking University Shenzhen HospitalShenzhen 518036Guangdong ProvinceChina National and Local Joint Engineering Research Center of Orthopaedic Biomaterials Peking University Shenzhen HospitalShenzhen 518036Guangdong ProvinceChina

Tendon is a mechanosensitive tissue that transmits force from muscle to *** loading contributes to maintaining the homeostasis and adaptation of tendon,but aberrant loading may lead to injury or failed *** is shown that stem cells respond to mechanical loading and play an essential role in both acute and chronic injuries,as well as in tendon *** the process of mechanotransduction,mechanical loading is detected by mechanosensors that regulate cell differentiation and proliferation via several signaling *** order to better understand the stem-cell response to mechanical stimulation and the potential mechanism of the tendon repair process,in this review,we summarize the source and role of endogenous and exogenous stem cells active in tendon repair,describe the mechanical response of stem cells,and finally,highlight the mechanotransduction process and underlying signaling pathways.

关键词： Stem cells Mechanical loading Tendon repair Mechanotransduction

Spray characteristics of different regions downstream of a swirl cup

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Chinese Journal of Aeronautics 2024年第8期37卷 124-143页

作者： Donghui WANG yong huang Hongzhou ZHANG Collaborative Innovation Center of Advanced Aero-Engine School of Energy and Power EngineeringBeihang UniversityBeijing 102206China National Key Laboratory of Science and Technology School of Energy and Power EngineeringBeihang UniversityBeijing 102206China

The spray characteristics of different regions downstream of swirl cups play a critical role in cold start and re-ignition of gas *** spray measurements were performed at the fuel pressures of 0.5,0.8,1.0,1.5,and 2.0 MPa and the fuel temperatures of-23,-13,-3,7,17 and 27℃,*** droplet size,droplet velocity,droplet number,and instantaneous spatial spray image of sprays from an aviation kerosene Jet-A were measured using a two-component phase Doppler particle analyzer and a digital off-axis holography *** the fuel pressure and temperature increase,the Sauter Mean Diameter(SMD)and spray non-uniformity of the Spray Shear Layer(SSL)gradually *** the fuel pressure increases,the SMD and spray non-uniformity of the Central Toroidal Recirculation Zone(CTRZ)gradually decrease,and the slopes of these curves both *** the fuel pressure increases,the SMD and spray nonuniformity of the CTRZ rapidly decrease at the fuel temperature of23℃,while slightly decrease at the fuel temperature of 27℃.The droplets in the CTRZ come from 3 different sources:simplex nozzle,venturi,and outside the *** the fuel pressure increases,the proportion of droplets recirculated from outside the CTRZ *** study proposed the concept of the“pressure critical point”for the swirl *** the fuel temperature decreases,the proportion of droplets recirculated from outside the CTRZ increases below the critical pressure,while decreases above the critical *** addition,through the models of liquid film formation and breakup on the curved cylindrical wall,a semi-theoretical model was established to predict the SMD of SSL for swirl *** prediction uncertainty of this model is less than 6%for all 14 conditions in this paper.

关键词： Swirl cup Sauter Mean Diameter(SMD) Spray non-uniformity Atomizing regions Semi-theoretical prediction Spray nozzles

Insight into structural degradation of NCMs under extreme fast charging process

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Rare Metals 2024年第1期43卷 41-50页

作者： Yu Tang Xing-Yu Wang Jin-can Ren Bo-Wen Chen Zhi-yong huang Wei Wang Ya-Lan huang Bing-Hao Zhang Si Lan Zhang-Long He Qi Liu Hao He Department of Physics City University of Hong KongHong Kong 999077China Shenzhen Research Institute City University of Hong KongShenzhen 518057China College of Materials Science and Engineering Changsha University of Science&TechnologyChangsha 410114China

Lithium-ion batteries(LIBs)with extreme fast charging(XFC)capability are considered an effective way to alleviate range anxiety for electric vehicle(EV)*** to the high ionic and electronic conductivity of LiNi_(x)Co_(y)Mn_zO_(2)(x+y+z=1,NCM)cathodes,the inevitable Li plating of graphite in NCM|graphite cell is usually identified as a key bottleneck for XFC ***,the capacity decay mechanism of cathode materials under XFC has not been fully *** this work,three typical NCM cathode materials with different Ni fractions were chosen and their electrochemical performances under XFC associated with structural evolution were investigated.A faster capacity decay of NCMs under XFC conditions is observed,especially for Ni-rich ***-situ X-ray diffraction(XRD)reveals that the multiple caxis parameters appear at the high-voltage regions in Nirich NCMs,which is probably triggered by the larger obstruction of Li(de)***,NCMs with moderate Ni fraction also present a similar trend under XFC *** phenomenon is more detrimental to the structural and morphological stability,resulting in a faster capacity decay than that under low current *** work provides new insight into the degradation mechanism of NCMs under XFC conditions,which can promote the development of NCM cathode materials with XFC capability.

关键词： Lithium-ion batteries Ni fraction Extreme fast charging Structural evolution