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Modulatory effects of diallyl sulfide against testosteroneinduced oxidative stress in Swiss albino mice

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Asian Journal of Andrology 2006年第6期8卷 719-723页

作者： Sahdeo Prasad Neetu Kalra yogeshwer shukla Environmental Carcinogenesis Division Industrial Toxicology Research CentreLucknow 226001India

Aim： To investigate the protective effect of diallyl sulfide （DAS）, a constituent of garlic, against testosterone-induced oxidative stress in male Swiss albino mice. Methods： The animals were given low （250 mg/animal） and high dose （500 mg/animal） of DAS in corn oil for 7 days along with testosterone （5 mg/kg body weight, i.p.）. At the end of the study period, the prostate and the liver were dissected to determine various antioxidant enzyme levels （catalase, superoxide dismutase, glutathione reductase, glutathione-s-transferase） and lipid peroxidation. Results： In testosterone treated mice, depleted antioxidant enzyme level was accompanied with enhancement in lipid peroxidation in prostate and liver. DAS significantly restored the testosterone-induced antioxidant enzymes and lipid peroxidation in the both organs. These changes appear to be mediated by the antioxidant-enhancing effects of DAS. Conclusion： The results of the present study suggest that DAS is effective in exerting antioxidant effects by inhibiting testosterone-induced oxidative stress and might be helpful in preventing prostate cancer.

关键词： oxidative stress antioxidants lipid peroxidation diallyl sulfide testosterone

Antitumor Activity of Diallyl Sulfide in Two-StageMouse Skin Model of Carcinogenesis

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Biomedical and Environmental Sciences 1998年第3期11卷 258-263页

作者： ALKA SINGH AND yogeshwer shukla(Laboratory of Environmental Chrcinogenesis, Industrial ToxicologyResearch Centre, P. O. Box 80, M. G. Marg,Lucknow-226 001, India) Laboratory of Environmental Carcinogenesis Industrial Toxicology Research Centre Lucknow India.

It has been reported that diallyl sulfide (DAS), a sulfur-containing volatile compound in garlic (Allium sativum ), exerts anticarcinogenic activity in various rodent tumor models. In the present study, the antitumor property of DAS was tested in Swiss albino mice in the two steqe initiation-promotion mouse skin carcinogenesis. Skin cancers were initiated topically with a single subcarcinOgenic dose (52μg) of 7, 12-dimethyl benz (a) anthracene (DMBA). Promotion was performed by twice weekly applications of 12-O-tetradecanoyl phorbol-13-acetate (TPA) at a dose of 5μg/animal for 32 weeks. DAS was applied topically (250μg/animal) thrice weekly for 3 weeks for anti-initiating and 1 h prior to each promotion treatment for anti-promoting studies. The results showed that the treatment schedule of DAS can effectively delay the onset of tumorigenesis and reduce the cumulative number of tumors and the average number of tumors per mouse. In groups in which DAS applied prior to initiation or promotion, a significant population of the aniinals remained tumor-free till the termination of experiment. These findings suggest that DAS can effectively inhibit chemically induced mouse skincarcinogenesis.

关键词： MNNG Activity Antitumor Activity of Diallyl Sulfide in Two-StageMouse Skin Model of Carcinogenesis

Histochemical Localization of Glutathione Dependent NBT-Reductase in Mouse Skin

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Biomedical and Environmental Sciences 2001年第3期14卷 237-240页

作者： yogeshwer shukla 1Environmental Carcinogenesis Division, Industrial Toxicology Research Centre, Post Box 80, M. G. Marg, Lucknow-226 001, India Environmental Carcinogenesis Division，Industrial Toxicology Research Centre 印度 Lucknow-22

Objective Localization of the glutathione dependent Nitroblue tetrazolium (NBT) reductase in fresh frozen sections of mouse skin and possible dependence of NBT reductase on tissue thiol levels has been investigated. Methods The fresh frozen tissue sections (8m thickness) were prepared and incubated in medium containing NBT, reduced glutathione (GSH) and phosphate buffer. The staining for GSH was performed with mercury orange. Results The activity of the NBT-reductase in mouse skin has been found to be localized in the areas rich in glutathione and actively proliferating area of the skin. Conclusion The activity of the NBT-reductase seems to be dependent on the glutathione contents.

关键词： Animals Cell Division Female Glutathione Mice Nitroblue Tetrazolium Oxidoreductases Skin

Inhibition of Carcinogen-induced Activity of γ-Glutamyl Transpeptidase by Certain Dietary Constituents in Mouse Skin

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Biomedical and Environmental Sciences 1999年第1期12卷 110-115页

作者： yogeshwer shukla ALKA SINGH AND BHAWNA SRIVASTAVA(Environmental Carcinogenesis Division, Industrial Toxicology Research Centre,P. O. Box 80, M. C. Marg, Lucknow-226 001, India) Industrial Toxicology Research Centre 印度

Cancer chemoprevention, a desirable and important facet of biomedical research, Provides a practical approach to identify potentially useful inhibitors of cancer development, and offers an opportunity to study the mechanism of carcinogenesis. During the recent past a nuInber of compounds have been tested for their anticarcinogenic potential specially constituents of human diet. The enzyme y-glutamyl transpeptidase (CGT), which catalyses the transfer of glutamyl gnups of peptides to other peptides and amino acids, has been proposed as a marker of cell proliferation and neoplasia. It also serves as a tool to evaluate the carcinogenic and cocareinogenic poential of environmental toxicants. In the present investigations, GGT activity induced by carcinogenic polycyclic aromatic hydrocarbons, viz. 7, 12-dimethyl-benz(a) anthracene (DMBA) and benzo(a)Pyrene (BaP was significantly inhibited by diallylsulfide (DAS) and indole-3-carbinol (13C) in mouse skin. DAS and 13C are Constituents of garlic. A significant inhibition in GGT levels was also observed in a strong mitogen (12-o-tetradecanoyl phorbol-13-acelate) induced activity by pretreatment of DAS/13C in mouse skin. Therefore these dielary constituents seem to be strong modifiers of chemically induced carcinogenesis.

关键词： inhibition carcinogen γ-glutamyl mouse skin

Chemopreventive Effects of Black Tea Polyphenols in Mouse Skin Model of Carcinogenesis

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Biomedical and Environmental Sciences 1998年第4期11卷 307-313页

作者： SEEMA JAVED N. K. MEHROTRA AND yogeshwer shukla(Laboratory of Environmental Chrcinogenesis, Industrial Toxicology Research Centre, M. G. Marg, P. O. Box 80,Lucknow - 226001, India) Lab. of Environmental Carcinogenesis Indust. Toxicology Research Centre M. G. Marg P. O. Box 80 Lucknow - 226001 India

In the present investigations, the antitumorigenic effect of black tea polyphenols (BTP) in twcrstage mouse skin model of carcinogenesis was studied. The animals were initiated with a single 'subcarcinogenic' topical dose (52 μg/200 μl acetone ) of 7, 12-dimethylbenzanthracene (DMBA). To evaluate the anti-tumour initiating activity, BTP was topically applied twice a week for three weeks prior to DMBA application, followed by topical treatment with 12-o-tetradecanoyl phorbol-13-acetate (TPA) (5 μg/200 μl acetone, 2x/wk. ) as promoter. For evaluation of antitumor promoting activity, BTP was applied prior to each treatment of TPA. BTP application showed marked inhibitory effect as antitumour initiator as well as antitumour promoter in mouse skin medel of two-stage carcinogenesis. Since initiation involves genetic pathway and tumour promotion involves epigenetic pathway, it seems that BTP exerts its antitumorigenic effect by altering both genetic and epigenetic pathways

关键词： Chemopreventive Effects of Black Tea Polyphenols in Mouse Skin Model of Carcinogenesis