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A bHLH transcription factor,CsSPT,regulates high-temperature resistance in cucumber

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Horticultural Plant Journal 2024年第2期10卷 503-514页

作者： Yonggui Liang Chenyu Yang Fangyan Ming Bingwei Yu Zhihua Cheng yixi wang Zhengkun Qiu Xiaolan Zhang Bihao Cao Shuangshuang Yan Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(South China) Ministry of Agriculture and Rural AffairsGuangdong Vegetable Engineering and Technology Research CenterCollege of HorticultureSouth China Agricultural UniversityGuangzhouGuangdong 510642China State Key Laboratories of Agrobiotechnology Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable CropsMOE Joint Laboratory for International Cooperation in Crop Molecular BreedingChina Agricultural UniversityBeijing 100193China

High-temperature stress threatens the growth and yield of crops. Basic helix-loop-helix(bHLH) transcription factors(TFs) have been shown to play important roles in regulating high-temperature resistance in plants. However, the bHLH TFs responsible for high-temperature tolerance in cucumbers have not been identified. We used transcriptome profiling to screen the high temperature-responsive candidate bHLH TFs in cucumber. Here, we found that the expression of 75 CsbHLH genes was altered under high-temperature stress. The expression of the CsSPT gene was induced by high temperatures in TT(Thermotolerant) cucumber plants. However, the Csspt mutant plants obtained by the CRISPR-Cas9 system showed severe thermosensitive symptoms, including wilted leaves with brown margins and reduced root density and cell *** Csspt mutant plants also exhibited elevated H_(2)O_(2) levels and down-regulated photosystem-related genes under normal ***, there were high relative electrolytic leakage(REC), malondialdehyde(MDA), glutathione(GSH), and superoxide radical(O_(2)^(·-)) levels in the Csspt mutant plants, with decreased Proline content after the high-temperature treatment. Transcriptome analysis showed that the photosystem and chloroplast activities in Csspt mutant plants were extremely disrupted by the high-temperature stress compared with wildtype(WT) plants. Moreover, the plant hormone signal transduction, as well as MAPK and calcium signaling pathways were activated in Csspt mutant plants under high-temperature stress. The HSF and HSP family genes shared the same upregulated expression patterns in Csspt and WT plants under high-temperature conditions. However, most bHLH, NAC, and bZIP family genes were significantly down-regulated by heat in Csspt mutant plants. Thus, these results demonstrated that CsSPT regulated the high-temperature response by recruiting photosynthesis components, signaling pathway molecules, and transcription factors. Our results pro

关键词： Cucumber bHLH CsSPT Photosynthesis High temperature

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热带中西太平洋大眼金枪鱼的繁殖生物学特性

水产学报 2024年第6期48卷 36-47页

作者：张健刘文俊王佚兮王啸石建高叶旭昌上海海洋大学海洋科学学院上海海洋大学国家远洋渔业工程技术研究中心上海海洋大学大洋渔业资源可持续开发教育部重点实验室上海海洋大学农业农村部大洋渔业开发重点实验室上海开创远洋渔业有限公司中国水产科学研究院东海水产研究所

利用漂流金枪鱼延绳钓作业方式，于2018年5月—2019年3月对热带中西太平洋海域(WCPO,2°03′～11°17′S,163°14′～173°35′E)的大眼金枪鱼进行了取样，对其中886尾进行了繁殖生物学测定。使用广义线性模型(GLM)和多元有序Logistic... 详细信息

利用漂流金枪鱼延绳钓作业方式，于2018年5月—2019年3月对热带中西太平洋海域(WCPO,2°03′～11°17′S,163°14′～173°35′E)的大眼金枪鱼进行了取样，对其中886尾进行了繁殖生物学测定。使用广义线性模型(GLM)和多元有序Logistic回归模型，结合圆形统计方法，分析个体叉长等生物因素、渔获水深和月度等时空因素对繁殖生物学参数的影响。结果显示，雄性和雌性平均叉长分别为(132.2±19.1) cm和(125.4±16.8) cm。取样群体中，雌雄比为0.54±0.74，且叉长与雌雄比具有负相关(r=-0.263,P=0.016)，叉长大于140 cm后，雄性占主导。GLM模型结果显示，性腺指数(GSI)与叉长之间存在显著的二次方关系，即个体GSI随着叉长增加，当雄性和雌性叉长分别达到130～140 cm和140～150 cm后，GSI随叉长下降。基于圆形统计发现，月度对个体GSI具有显著影响，其中雄性GSI上半年较下半年高(正弦显著，P<0.001)，而雌性GSI年末和年初高于年中(余弦显著，P=0.021)。有序多元Logistic模型结果显示，性腺成熟度随叉长显著增加(P<0.001)，雄性性腺成熟度在5—7月较高(余弦显著，P=0.004)。此外，栖息水层对雄性个体的性腺成熟度具有显著的影响(正相关，P<0.001)。雄性和雌性个体50%性成熟叉长分别为(96.13±1.24) cm和(104.93±2.60) cm。研究结果可为更好地了解热带WCPO大眼金枪鱼的繁殖生物学特征、科学养护热带WCPO大眼金枪鱼资源提供参考。

关键词：大眼金枪鱼繁殖生物学圆形统计多元有序Logistic模型热带中西太平洋

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甲状腺抗体异常在重症肌无力中的研究进展

中国实用神经疾病杂志 2024年第5期27卷 634-638页

作者：黄雅舒王一晰李梦岚四川大学华西医院四川成都610000

重症肌无力(MG)是一种典型的抗体介导的自身免疫性疾病,因较差免疫的存在,常合并多种其他自身免疫性疾病。目前MG患者以合并甲状腺功能异常最为常见,且多伴有甲状腺抗体异常,而有学者发现,部分甲状腺功能正常患者自身抗体也处于阳性状态... 详细信息

重症肌无力(MG)是一种典型的抗体介导的自身免疫性疾病,因较差免疫的存在,常合并多种其他自身免疫性疾病。目前MG患者以合并甲状腺功能异常最为常见,且多伴有甲状腺抗体异常,而有学者发现,部分甲状腺功能正常患者自身抗体也处于阳性状态,且患者甲状腺疾病相关症状严重程度与自身抗体水平有关。本文就MG与甲状腺抗体异常的关系做一综述。

关键词：自身免疫系统疾病甲状腺抗体异常阳性甲状腺功能异常重症肌无力

孕妇药物依赖的胎儿权益与母体自主冲突之思考

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医学与哲学 2024年第14期45卷 19-22页

作者：龚韩湘李静华江慧琳王一西高琪佩杨亚军广州医科大学附属第二医院医务科广东广州510260 广州医科大学公共卫生学院广东广州511495 广州医科大学附属第二医院急诊科广东广州510260 广州医科大学附属第二医院药学部广东广州510260 广州医科大学附属第二医院科研管理科广东广州510260 广州医科大学附属第二医院人事培训科广东广州510260

聚焦医学伦理学视角下孕妇药物依赖及其对胎儿健康的潜在影响,探索胎儿权利与母体自主权之间的伦理平衡及在医疗与社会法律层面的复杂挑战。探讨了胎儿权益与母体自主间的伦理张力,分析了药物依赖的成因,包括治疗性药物使用和药物滥用,... 详细信息

聚焦医学伦理学视角下孕妇药物依赖及其对胎儿健康的潜在影响,探索胎儿权利与母体自主权之间的伦理平衡及在医疗与社会法律层面的复杂挑战。探讨了胎儿权益与母体自主间的伦理张力,分析了药物依赖的成因,包括治疗性药物使用和药物滥用,进而审视全球孕妇药物依赖现状及其影响。同时,评估了医学伦理四大原则在两种情境中的应用挑战,以及法律在定义和处理该伦理问题时的作用与临床实践中的差异。强调了公共卫生策略在解决孕妇药物依赖问题中的重要性,并提出了具体实施策略,以期为医疗从业者和政策制定者提供理论与实践指导。

关键词：孕妇药物依赖胎儿权益母体自主

穴位埋线治疗非酒精性脂肪性肝病的临床疗效观察

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上海中医药大学学报 2024年第3期38卷 45-49页

作者：周峰峰陈羽蔡浩亮王依希卓蕴慧杨昌琨上海中医药大学附属市中医医院上海200070 上海中医药大学附属曙光医院上海201203 上海开元骨科医院上海200120

目的:探讨穴位埋线治疗非酒精性脂肪性肝病(NAFLD)临床疗效。方法:纳入非酒精性脂肪性肝病患者130例,采用随机数字表分为普通针刺组和穴位埋线组,每组各65例。两组患者分别于0、1、3、6月检测患者血脂、肝功能等指标,对比0、6月核磁共... 详细信息

目的:探讨穴位埋线治疗非酒精性脂肪性肝病(NAFLD)临床疗效。方法:纳入非酒精性脂肪性肝病患者130例,采用随机数字表分为普通针刺组和穴位埋线组,每组各65例。两组患者分别于0、1、3、6月检测患者血脂、肝功能等指标,对比0、6月核磁共振成像(MRI)肝脏脂肪含量定量分析变化情况,评估穴位埋线的治疗效果;于0、3、6月检测患者血浆脂联素(ADP)、过氧化物酶体增殖激活α受体(PPAR-α)活性,观察血脂和ADP、PPAR-α活性变化的相关性。结果:(1)两组患者治疗后各阶段与治疗前比较,肝功能、血脂水平均有改善,且穴位埋线组疗效优于普通针刺组(P<0.01);(2)ADP、PPAR-α与治疗前比较显著上升(P<0.01),治疗后两组ADP、PPAR-α水平比较差异显著(P<0.01);(3)治疗后,两组MRI肝脏脂肪含量定量分析比较差异显著(P<0.05),穴位埋线组MRI肝脏脂肪含量定量分析明显优于普通针刺组。结论:穴位埋线疗法能有效改善肝脏脂肪沉积,且疗效优于普通针刺。

关键词：非酒精性脂肪性肝病穴位埋线针灸临床观察

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腹壁脂膜切除术后并发症的分类及防治进展

中国美容医学 2024年第8期33卷 194-198页

作者：王禹栋(综述) 王一希(综述) 赵焓杏(综述) 岑瑛(综述) 李正勇(审校) 张振宇(审校) 四川大学华西医院整形外科/烧伤科四川成都610041

腹壁脂膜切除术是帮助肥胖人群去除腹壁多余皮肤和脂膜的功能性手术,该术式改善腹壁外形的效果显著,让肥胖患者受益匪浅,因此手术需求日趋增多。然而,在肥胖患者中实施腹壁脂膜切除术后,局部和全身并发症发生率较高,严重影响患者的生命... 详细信息

腹壁脂膜切除术是帮助肥胖人群去除腹壁多余皮肤和脂膜的功能性手术,该术式改善腹壁外形的效果显著,让肥胖患者受益匪浅,因此手术需求日趋增多。然而,在肥胖患者中实施腹壁脂膜切除术后,局部和全身并发症发生率较高,严重影响患者的生命安全和手术效果,需要引起高度重视。本文结合国内外最新文献报道,对腹壁脂膜切除术后常见并发症类型、预防和治疗措施进行了总结分析,希望能为今后的临床工作提供借鉴和参考。

关键词：脂膜切除术肥胖并发症腹壁成形术预防

The Variation of Plankton Community Structure in Artificial Reef Area and Adjacent Waters in Haizhou Bay

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Journal of Ocean University of China 2024年第1期23卷 264-276页

作者： GAO Shike SHI yixi LU Yanan ZHANG Shuo College of Marine Sciences Shanghai Ocean UniversityShanghai 201306China Joint Laboratory for Monitoring and Conservation of Aquatic Living Resources in the Yangtze Estuary Shanghai 200000China

Plankton are an important component of marine protected areas(MPAs),and its communities would require much smaller interpatch distances to ensure connection among *** to the survey from MPAs dominated by artificial reefs and adjacent waters(estuary area(EA),aquaculture area(AA),artificial reef area(ARA),natural area(NA)and comprehensive effect area(CEA))in Haizhou Bay in spring and autumn,we analyzed phyto-zooplankton composition,abundance and biomass,and correlation with hydrologic variables to gain information about the forces that structure the *** results showed that the dominant zooplankton were copepods(spring,98.9%;autumn,94.2%),while the phytoplankton were mainly composed of Bacillariophyta(spring,61.8%;autumn,95.6%).The RDA results showed that temperature,salinity and depth highly associated with the distribution and composition of plankton species among the habitats than other factors in spring;temperature,Chla and DO had the strongest influence in *** zooplankton in the ARA and AA ecosystems basically contained the same species as those in other habitats,and each habitat also exhibited a relatively unique combination of plankton *** structures of the EA zooplankton in spring and the EA phytoplankton in both seasons were much different than other habitats,which may have been caused by factors such as currents and *** concluded that there exists similarity of the plankton community between artificial reef area and adjacent waters,whereas the EAs may be relatively independent ***,these interaction between plankton community should be considered when designing MPA networks,and ocean circulations should be considered more than the environmental factors.

关键词： zooplankton phytoplankton seasonal variation environmental factor artificial reef

3D printing of personalized polylactic acid scaffold laden with GelMA/autologous auricle cartilage to promote ear reconstruction

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Bio-Design and Manufacturing 2023年第4期6卷 451-463页

作者： Xingyu Gui Zhiyu Peng Ping Song Li Chen Xiujuan Xu Hairui Li Pei Tang yixi wang Zixuan Su Qingquan Kong Zhenyu Zhang Zhengyong Li Ying Cen Changchun Zhou Yujiang Fan Xingdong Zhang National Engineering Research Center for Biomaterials Sichuan UniversityChengdu 610064China Department of Thoracic Surgery West China HospitalSichuan UniversityChengdu 610041China Analytical and Testing Center Sichuan UniversityChengdu 610064China College of Biomedical Engineering Sichuan UniversityChengdu 610064China Department of Burn and Plastic Surgery West China HospitalSichuan UniversityChengdu 610041China Department of Orthopedics West China HospitalSichuan UniversityChengdu 610041China

At present,the clinical reconstruction of the auricle usually adopts the strategy of taking autologous costal *** method has great trauma to patients,poor plasticity and inaccurate ***-dimensional(3D)printing technology has made a great breakthrough in the clinical application of orthopedic *** study explored the combination of 3D printing and tissue engineering to precisely reconstruct the ***,a polylactic acid(PLA)polymer scaffold with a precisely customized patient appearance was fabricated,and then auricle cartilage fragments were loaded into the 3D-printed porous PLA scaffold to promote auricle *** vitro,gelatin methacrylamide(GelMA)hydrogels loaded with different sizes of rabbit ear cartilage fragments were studied to assess the regenerative activity of various autologous cartilage *** vivo,rat ear cartilage fragments were placed in an accurately designed porous PLA polymer ear scaffold to promote auricle *** results indicated that the chondrocytes in the cartilage fragments could maintain the morphological phenotype in *** three months of implantation observation,it was conducive to promoting the subsequent regeneration of cartilage in *** autologous cartilage fragments combined with 3D printing technology show promising potential in auricle reconstruction.

关键词： Microtia 3D printing Polylactic acid(PLA)polymer scaffolds Gelatin methacrylamide Cartilage reconstruction

Novel robust cellulose-based foam with pH and light dual-response for oil recovery

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Frontiers of Materials Science 2018年第2期12卷 118-128页

作者： Qian wang Guihua MENG Jianning WU yixi wang Zhiyong LIU Xuhong GUO School of Chemistry and Chemical Engineering Shihezi University/Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan/Key Laboratory of Materials-Oriented Chemical Engineering of Xinjiang Uygur Autonomous Region/Engineering Research Center of Materials-Oriented Chemical Engineering of Xinjiang Bingtuan Shihezi 832003 China State Key Laboratory of Chemical Engineering East China University of Science and Technology Shanghai 200237 China

We fabricated pH and light dual-responsive adsorption materials which could induce the transition of surface wettability between hydrophobicity and hydrophilicity by using ATRPo The structure and morphology of adsorption materials were confirmed by ATR-FTIR, XPS, TGA and SEM. It showed that the modified cellulose （CE）- based foam was hydrophobic, which can adsorb a range of oils and organic solvents in water under pH = 7.0 or visible light irradiation （λ〉500 nm）. Meanwhile, the wettability of robust CE-based foam can convert hydrophobicity into hydrophilicity and underwater oleophobicity under pH = 3.0 or UV irradiation （λ = 365 nm）, giving rise to release oils and organic solvents. Most important of all, the adsorption and desorption processes of the modified CE-based foam could be switched by external stimuli. Furthermore, the modified CE-based foam was not damaged and still retained original performance after reversible cycle repeated for many times with variation of surface wettability. In short, it indicates that CE-based foam materials with switchable surface wettability are new responsive absorbent materials and have owned potential application in the treatment of oil recovery.

关键词： cellulose-based foam dual-responsive adsorption materials switchable wettability oil recovery