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结缔组织生长因子介导的PI3K/Akt和RhoA/ROCK信号通路在百草枯中毒致肺泡上皮细胞间充质化的作用

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职业卫生与应急救援 2024年第3期42卷 295-301页

作者：苏艺伟李广珍方文馨唐侍豪王致广州市第十二人民医院(广州市职业病防治院) 广东广州510620 广州医科大学职业与环境卫生研究所广东广州511436 暨南大学基础医学与公共卫生学院广东广州510632

目的探讨结缔组织生长因子(connective tissue growth factor,CTGF)与PI3K/Akt、RhoA/ROCK信号通路在百草枯(paraquat,PQ)中毒致肺泡上皮细胞间充质化(epithelial-mesenchymal transition,EMT)中的作用机制。方法将RLE-6TN细胞分成4组,分别为对照组、PQ组、无关质粒组和干扰质粒组,干扰质粒组细胞转染含对CTGF具有高抑制效应的CTGF-shRNA质粒,无关质粒组转染对CTGF无干扰效应的质粒,检测分析各组细胞PI3K/Akt和RhoA/ROCK信号通路及EMT相关分子mRNA和蛋白的表达;为进一步阐明两条信号通路在PQ中毒致RLE-6TN细胞EMT中的作用关系,将RLE-6TN细胞分成5组,分别为对照组、PQ组、PI3K抑制组、RhoA抑制组、联合抑制组,检测分析各组细胞EMT相关分子mRNA及蛋白的表达情况。结果特异性抑制CTGF的表达后,干扰质粒组细胞CTGF、PI3K、Akt、RhoA、ROCK mRNA及蛋白的表达量较PQ组和无关质粒组降低(P<0.05/6);进一步检测RLE-6TN细胞EMT相关分子表达情况,结果显示,与PQ组和无关质粒组相比,干扰质粒组细胞E-cadherin mRNA和蛋白表达量升高,α-SMA mRNA和蛋白表达量降低(P<0.05/6)。利用特异性抑制剂阻断信号通路活性后,与PQ组相比,PI3K抑制组、RhoA抑制组、联合抑制组细胞α-SMA mRNA和蛋白表达量降低(P<0.05/10),而E-cadherin mRNA和蛋白表达量升高(P<0.05/10);与PI3K抑制组和RhoA抑制组相比,联合组细胞E-cadherin蛋白表达量升高(P<0.05/10),α-SMA蛋白表达量降低(P<0.05/10)。结论CTGF可通过激活PI3K/Akt和RhoA/ROCK信号通路,促进PQ致肺泡上皮细胞EMT过程,且PI3K/Akt通路和RhoA/ROCK通路在PQ致肺泡上皮细胞EMT中具有协同作用。

关键词：百草枯肺泡上皮细胞间充质化结缔组织生长因子

人工和仿生栽培暗褐网柄牛肝菌主要虫害及防治

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热带农业科技 2020年第1期43卷 24-26,35页

作者：王文兵刘静杨天伟方艺伟高锋张春霞云南省热带作物科学研究所

暗褐网柄牛肝菌是牛肝菌中目前唯一能人工栽培和仿生栽培的食用菌,近年来随着人工栽培规模的扩大,虫害已成为导致其产量和品质下降的主要因素之一。本文对暗褐网柄牛肝菌人工和仿生栽培过程中主要害虫种类进行调查,结果表明危害暗褐网... 详细信息

暗褐网柄牛肝菌是牛肝菌中目前唯一能人工栽培和仿生栽培的食用菌,近年来随着人工栽培规模的扩大,虫害已成为导致其产量和品质下降的主要因素之一。本文对暗褐网柄牛肝菌人工和仿生栽培过程中主要害虫种类进行调查,结果表明危害暗褐网柄牛肝菌的主要害虫种类有蜗牛、蛞蝓、菌螨、白蚁等4大类,其中螨类主要危害菇房人工栽培的暗褐网柄牛肝菌,蜗牛、蛞蝓、白蚁主要危害仿生栽培的牛肝菌。

关键词：暗褐网柄牛肝菌虫害综合防治

苯并-3-氮杂环庚酮-1衍生物及N-二烷氧基磷酰基-N-取代苯乙基甘氨酸衍生物的电子电离质谱

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分析化学 1986年第7期 481-486页

作者：卢紫莼严琳徐梦山奚士康赵玉芬方一苇中国科学院化学研究所中国科学院化学研究所北京

应用高分辨质谱,准确质量测定和亚稳态离子的测定结果,对五个苯并-3-氮杂环庚酮-1衍生物及六个N-二烷氧基磷酰基-N-取代苯乙基甘氨酸衍生物的断键机理进行了研究。在EIMS条件下,前者的分子离子峰比后者强;N原子上发生电离的几率最大;除... 详细信息

应用高分辨质谱,准确质量测定和亚稳态离子的测定结果,对五个苯并-3-氮杂环庚酮-1衍生物及六个N-二烷氧基磷酰基-N-取代苯乙基甘氨酸衍生物的断键机理进行了研究。在EIMS条件下,前者的分子离子峰比后者强;N原子上发生电离的几率最大;除磷酰基上取代基为甲基的两个化合物外,其他九个化合物均含有m/z110（CH5O3NP）碎片离子;由于受磷酰基的影响,这些化合物都发生C—N键特征断裂。

关键词：环庚酮烷氧基衍生物磷酰基苯乙基氮杂甘氨酸神经递体苯并取代基

Nutrition Metabolism and Infections

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Infectious Microbes & Diseases 2021年第3期3卷 134-141页

作者： fang Yang Yi Yang Lingchan Zeng yiwei Chen Gucheng Zeng Department of Microbiology Zhongshan School of MedicineKey Laboratory for Tropical Diseases Control of the Ministry of EducationSun Yat-sen UniversityGuangzhouGuangdongChina Clinical Research Center Department of Medical Records ManagementGuanghua School of StomatologyHospital of StomatologySun Yat-sen UniversityGuangzhouGuangdongChina

Infection and nutrition are intricately interacted and further influence human *** are a worldwide health problem and malnutrition plays a significant role in the emergence of *** evidence suggests that the optimization of dietary nutrients intake is crucial in maintaining systemic immunity and may help improve resistance to *** this review,we explore a wide range of topics including interactions between nutrients and various infectious *** also discuss the role of diet-induced gut microbiota in the infection-nutrition cycle and review how dietary-microbiome crosstalk may affect disease development and progression,which may provide an attractive option to the design of a diet leading to favorable outcomes in the *** will also present evidence and propose mechanisms of nutrients that may specifically modulate host immunity and metabolism to infectious pathogens and also cover its influence on nutrition,focusing on *** provide representative nutrients in the present review based on their intensive studies and wide acceptance of their immuno-modulating ***,the efficacy and translational cost of their plausible utility to be anti-infective nutrients are also ***,we highlight the current progress and challenges to gain a better understanding of the research into microbiota,infectious diseases,and nutrition with an emphasis on future research directions.

关键词： nutrition infection metabolism

尿道板纵切卷管尿道成形术在尿道下裂中的应用及远期疗效

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临床小儿外科杂志 2020年第12期19卷 1082-1087页

作者：方一圩宋宏程孙宁张潍平田军李明磊黄澄如国家儿童医学中心首都医科大学附属北京儿童医院泌尿外科北京市100045

目的回顾性分析行尿道板纵切卷管尿道成形术(tubularized incised urethral plate urethroplasty,TIP)治疗的尿道下裂患者阴茎的特点并对其进行远期随访,总结TIP手术的适应证,了解其远期排尿、性功能及社会心理状况,以提高对TIP手术的... 详细信息

目的回顾性分析行尿道板纵切卷管尿道成形术(tubularized incised urethral plate urethroplasty,TIP)治疗的尿道下裂患者阴茎的特点并对其进行远期随访,总结TIP手术的适应证,了解其远期排尿、性功能及社会心理状况,以提高对TIP手术的认识。方法回顾性分析2004年1月至2011年12月初次行尿道下裂手术或尿道下裂术后再手术采用TIP手术治疗患者的临床资料,利用预先设计好的TIP手术后基本情况调查表通过电话随访及门诊复查,了解TIP手术远期疗效。结果共266例尿道下裂患者纳入本研究(占同期行尿道成形术2993例的8.89%),行TIP手术时年龄1岁至16岁9个月,平均5岁7个月。阴茎条件:术前无明显阴茎下弯74例,轻度阴茎下弯仅需包皮脱套及松解腹侧纤维组织矫正阴茎下弯106例,中度阴茎下弯需加做背侧白膜紧缩矫正阴茎下弯86例,无重度阴茎下弯;尿道板宽0.6~1.0 cm,均血供丰富、弹性好,纵切后宽1.0~1.4 cm。随访113例,随访率42.5%,失访153例。平均随访时间12.5年,末次随访时患者平均年龄为17岁10个月。83.2%的患者对阴茎外观满意,20.3%的患者存在排尿方面的问题,6.2%的患者存在阴茎下弯,所有患者均不存在阴茎勃起功能障碍、勃起疼痛等情况,98.2%的患者社会心理适应良好。结论TIP手术的应用需严格把握其适应证,阴茎下弯不严重的远端型和中间型尿道下裂,且尿道板发育良好为TIP手术的严格适应证。在正确把握其适应证的基础上,患者远期可获得较好的阴茎外观、排尿及性功能。

关键词：尿道下裂/外科学治疗结果手术后并发症阴茎/畸形

Development of a pressure coring system for the investigation of deep underground exploration

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International Journal of Mining Science and Technology 2023年第11期33卷 1351-1364页

作者： Da Guo Ling Chen Zhongya Zhou Dingming Wang yiwei Zhang Xun Yang Xin fang Xiaojun Wang Heping Lu Lin Dai Heping Xie State Key Laboratory of Intelligent Construction and Healthy Operation and Maintenance of Deep Underground Engineering Sichuan UniversityChengdu 610065China School of Mechanical Engineering Sichuan UniversityChengdu 610065China Guangdong Provincial Key Laboratory of Deep Earth Sciences and Geothermal Energy Exploitation and Utilization Institute of Deep Earth Sciences and Green EnergyCollege of Civil and Transportation EngineeringShenzhen UniversityShenzhen 518060China Petroleum Engineering Technology Research Institute of Sinopec Jianghan Oilfield Company Wuhan 430035China

To provide a more accurate evaluation of the scale of deep underground resources,a new pressure coring system was carefully developed and its strength and safety of the engineering were verified by theoretical analyses and numerical *** designed pressure coring system can obtain cores with length of 3 m and diameter of 50 mm at 70 *** results of the ball-drop operation experiment demonstrate that differential motion assembly can effectively cut a safety pin by applying a tensile force of 4852 N,and it can lift the core tube through the center pole to complete a series of mechanical actions to seal the ***,by maintaining pressures at 70 MPa for 30 min,the pressure sealing capacity of the system was ***,a core sample with a diameter of 50 mm was obtained through a core drilling experiment and the coring performance of the pressure coring system was *** study can not only enrich the existing onshore coring technology but also provide a theoretical guide and design criteria for the development of similar pressure coring systems to meet the demand for deeper underground exploration.

关键词： Underground exploration In-situ coring Pressure coring Oil and gas production

暗褐网柄牛肝菌废菌渣为主料栽培巨大口蘑试验

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热带农业科技 2023年第3期46卷 18-21,61页

作者：杨天伟刘静高锋何明霞许欣景方艺伟张春霞云南省热带作物科学研究所云南景洪666100

暗褐网柄牛肝菌人工栽培的生物学转化率较低,收菇后的废菌渣中余留了大量营养物质。为充分利用其废菌渣中的营养成分,减少资源浪费,研究采用不同比例暗褐网柄牛肝菌菌渣为主料栽培巨大口蘑。结果显示,采用暗褐网柄牛肝菌废菌渣为主料栽... 详细信息

暗褐网柄牛肝菌人工栽培的生物学转化率较低,收菇后的废菌渣中余留了大量营养物质。为充分利用其废菌渣中的营养成分,减少资源浪费,研究采用不同比例暗褐网柄牛肝菌菌渣为主料栽培巨大口蘑。结果显示,采用暗褐网柄牛肝菌废菌渣为主料栽培的巨大口蘑长势良好,菌丝生长速度在6.49±0.33~9.65±0.52 mm/d,鲜菇产量263.87±12.93~401.33±6.10 g/袋,生物学转化率达到57.36%~87.25%,表明巨大口蘑可以充分利用暗褐网柄牛肝菌废菌渣中的营养物质。建议栽培巨大口蘑时,暗褐网柄牛肝菌废菌渣用量控制在90%以内,并加强出菇管理。

关键词：暗褐网柄牛肝菌巨大口蘑资源利用废菌渣

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Screening potential P-glycoprotein inhibitors by combination of a detergent-free membrane protein extraction with surface plasmon resonance biosensor

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Acta Pharmaceutica Sinica B 2022年第7期12卷 3113-3123页

作者： Yuhong Cao Jiahao fang yiwei Shi Hui Wang Xiaofei Chen Yue Liu Zhenyu Zhu Yan Cao Zhanying Hong Yifeng Chai School of Pharmacy Second Military Medical UniversityShanghai Key Laboratory for Pharmaceutical Metabolites ResearchShanghai 200433China Zhejiang Institute for Food and Drug Control Hangzhou 310057China

P-glycoprotein(P-gp)highly expressed in cancer cells can lead to multidrug resistance(MDR)and the combination of anti-cancer drugs with P-gp inhibitor has been a promising strategy to reverse MDR in cancer *** this study,we established a label-free and detergent-free system combining surface plasmon resonance(SPR)biosensor with styrene maleic acid(SMA)polymer membrane proteins(MPs)stabilization technology to screen potential P-gp ***,P-gp was extracted from MCF-7/ADR cells using SMA polymer to form SMA liposomes(SMALPs).Following that,SMALPs were immobilized on an SPR biosensor chip to establish a P-gp inhibitor screening system,and the affinity between P-gp and small molecule ligand was *** methodological investigation proved that the screening system had good specificity and *** P-gp ligands were screened out from 50 natural products,and their affinity constants with P-gp were also *** in vitro cell verification experiments demonstrated that tetrandrine,fangchinoline,praeruptorin B,neobaicalein,and icariin could significantly increase the sensitivity of MCF-7/ADR cells to Adriamycin(Adr).Moreover,tetrandrine,praeruptorin B,and neobaicalein could reverse MDR in MCF-7/ADR cells by inhibiting the function of *** is the first time that SMALPs-based stabilization strategy was applied to SPR analysis *** polymer can retain P-gp in the environment of natural lipid bilayer and thus maintain the correct conformation and physiological functions of *** developed system can quickly and accurately screen small molecule ligands of complex MPs and obtain affinity between complex MPs and small molecule ligands without protein purification.

关键词： Styrene maleic acid P-Glycoprotein Surface plasmon resonance Membrane proteins Inhibitor screening Affinity calculation Natural products Multidrug resistance

动脉血HCO_(3)^(-)早期变化对急性百草枯中毒的预后价值

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中国工业医学杂志 2024年第2期37卷 123-126,137页

作者：李广珍苏艺伟方文馨刘移民唐侍豪王致广州市第十二人民医院/广州市职业病防治所职业环境与健康重点实验室广东广州510620 暨南大学基础医学与公共卫生学院公共卫生预防医学系

目的探索中毒早期患者动脉血碳酸氢根离子(HCO_(3)^(-))变化情况与急性百草枯(PQ)中毒死亡风险的关系。方法采用回顾性研究方法,收集2009年1月—2022年1月在广州市第十二人民医院住院治疗且符合研究条件的109例急性PQ中毒患者的临床资... 详细信息

目的探索中毒早期患者动脉血碳酸氢根离子(HCO_(3)^(-))变化情况与急性百草枯(PQ)中毒死亡风险的关系。方法采用回顾性研究方法,收集2009年1月—2022年1月在广州市第十二人民医院住院治疗且符合研究条件的109例急性PQ中毒患者的临床资料,根据患者中毒后60 d的随访结局,将研究对象分为生存组(54例)和死亡组(55例);通过潜类别轨迹模型分析患者中毒后3 d内动脉血HCO_(3)^(-)变化趋势,采用单因素分析与二元Logistic回归方法评估动脉血HCO_(3)^(-)动态变化轨迹,探索其对预测急性PQ患者死亡风险的价值。结果急性PQ中毒患者动脉血HCO_(3)^(-)动态变化有平稳轨迹(73例,66.97%)和升高轨迹(36例,33.03%)两种类型。死亡组患者HCO_(3)^(-)变化轨迹类型、性别、呕吐发生率、血液净化率与生存组相比,差异有统计学意义(P1,P<0.05),中毒后3 d内患者动脉血HCO_(3)^(-)变化为平稳轨迹者发生死亡的风险是升高轨迹者的4.434倍(P<0.05);预测模型准确率70.64%,特异度75.93%,敏感度65.45%。结论中毒早期动脉血HCO3-的变化趋势有助于判断急性PQ中毒的预后,患者中毒后3 d内HCO_(3)^(-)持续低值常预示中毒结局不良。

关键词：百草枯(PQ) 急性中毒碳酸氢根离子(HCO_(3)^(-)) 轨迹分析结局预测