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股前内侧皮瓣修复晚期喉癌保留舌骨上部分会厌的近全喉切除后环周缺损

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中国修复重建外科杂志 2024年第2期38卷 133-139页

作者：杨柳李文郑义涛纪琳四川大学华西医院耳鼻咽喉头颈外科成都610041 四川大学华西医院放射科成都610041

目的探讨股前内侧皮瓣修复晚期喉癌患者保留舌骨上部分会厌的近全喉切除后环周缺损,重建发音功能的可行性。方法回顾分析2019年8月—2022年10月5例晚期喉癌男性患者临床资料,年龄56~73岁,平均65岁。病程3~24个月,平均8个月。肿瘤按部位... 详细信息

目的探讨股前内侧皮瓣修复晚期喉癌患者保留舌骨上部分会厌的近全喉切除后环周缺损,重建发音功能的可行性。方法回顾分析2019年8月—2022年10月5例晚期喉癌男性患者临床资料,年龄56~73岁,平均65岁。病程3~24个月,平均8个月。肿瘤按部位分型:声门型2例、声门上型2例、声门下型1例;TNM分期:T4N0M0期3例、T4N1M0期1例、T4N2M0期1例;美国癌症联合会(AJCC)分期(2017)均为Ⅳ期。行保留舌骨上部分会厌的近全喉切除+双侧择区颈部淋巴结清扫术,以股前内侧皮瓣修复重建近全喉切除术后环周缺损,皮瓣切取范围6 cm×5 cm~8 cm×6 cm。术后4例患者补充放化疗,1例未补充放化疗等其他治疗。结果术后所有皮瓣均成活,无明显颈部感染。1例患者术后1个月经口进食发生轻微咽瘘,再次经胃管进食1周后咽瘘愈合。大腿供区创面Ⅰ期愈合。1例患者双侧颈部淋巴结转移,1例一侧颈部淋巴结转移;余3例双侧颈部淋巴结无转移。5例患者均获随访,随访时间12~36个月,平均27.6个月。4例患者发音清晰可闻、声音嘶哑,1例(例3)发音类似耳语。纤维喉镜检查示重建的喉入口呈裂隙状,喉入口以下重建的喉气管腔逐渐增大。术后1个月拔除胃管经口进食,无明显吸入性肺炎发生。4例患者可以间断堵管,堵管时间30 s~3 min,其中3例不堵管时也能发出明显声音,患者可与人进行无障碍语言交流;1例发音类似耳语患者不能堵管。初步嗓音分析显示,患者发音轻松自然,无明显憋气或吞气动作。随访期间患者均未拔管。结论股前内侧皮瓣修复晚期喉癌患者近全喉切除术后环周缺损可取得满意的发音效果,能够作为一种有效的发音重建方法;保留的部分会厌可能对防止术后误吸起到一定作用。

关键词：晚期喉癌近全喉切除环周缺损股前内侧皮瓣发音功能修复

Isolation of microsatellite loci and reliable genotyping using noninvasive samples of a critically endangered primate, Trachypithecus leucocephalus

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Integrative Zoology 2016年第4期11卷 250-262页

作者： Weiran WANG Yu QIAO yitao zheng Meng YAO School of Life Sciences Peking UniversityBeijingChina

Genetic information can be critical in identifying conservation priorities and developing conservation strategies.There is an urgent need for noninvasive genetic tools to study the wild populations of Asian colobine monkeys.The majority of these species are threatened with habitat destruction,population reduction and even extinction,but generally lack information on their genetic diversity and population structure.Genetic sampling and tissue collection have been scarce in these species owing to strict regulations on manipulation of endangered species,and the difficulties and risks associated with capturing these arboreal and fast-moving monkeys in the challenging environments that they inhabit.These difficulties have hindered the development of molecular genetic markers,which are usually derived from tissues or blood.In this study,we present a method for de novo microsatellite isolation and genotyping using DNA from noninvasive origins of a critically endangered Asian colobine,the white-headed langur(Trachypithecus leucocephalus).Genomic DNA isolated from hair was shown to be sufficient for microsatellite enrichment and isolation,with similar isolation efficiencies as from tissue DNA.We identified and characterized 20 polymorphic microsatellite loci,and evaluated their amplification success and genotyping reliability with 86 field-collected fecal samples.These results show that this panel of loci can produce reliable genotypes from fecal samples,and represent a useful tool for noninvasive investigation of genetic structure,individual identification and kinship assessment in this highly endangered species.Our approach can be applied to conservation genetic studies of other wild species that lack sequence information and tissue samples.

关键词： Asian colobine conservation genetics FIASCO hair scatology

Remarkable mechanochromism and force-induced thermally activated delayed fluorescence enhancement from white-light-emitting organic luminogens with aggregation-induced emission

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Chinese Chemical Letters 2022年第10期33卷 4536-4540页

作者： yitao zheng Lingqi Zuo Letian Zhang Zihao Huang Shufeng Li Zhan Yang Zhu Mao Suilian Luo Cong Liu Fengqiang Sun Guang Shi zhenguo Chi Bingjia Xu Key Laboratory of Theoretical Chemistry of Environment Ministry of Education/School of ChemistrySouth China Normal UniversityGuangzhou 510006China State Key Laboratory of Optoelectronic Materials and Technologies(Sun Yat-sen University) Guangzhou 510275China School of Chemistry Sun Yat-sen UniversityGuangzhou 510275China Shenzhen Institute of Advanced Electronic Materials Shenzhen Institutes of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China

The development of organic materials with white-light emission and thermally activated delayed fluorescence(TADF)properties in the solid state remain a challenge.Herein,a series of white-light-emitting organic luminogens have been developed and are found to show aggregation-induced delayed fluorescence(AIDF)characteristics.The AIDF emitters present dual-emission consisted of prompt fluorescence and TADF in the crystalline state.Their white-light emissions can be easily tuned by altering the chemical structure and connecting position of the heterocyclic aromatic substituent.Under the stimuli of mechanical force and solvent vapor,the compounds exhibit remarkable and reversible mechanochromism,in which their emission colors are switchable between white and yellow.Upon grinding,they also display linearly tunable luminescence colors,as well as force-induced TADF enhancement,which may be associated with the more compact molecular packing and the restriction of intramolecular motions.The results from time-resolved emission scanning and theoretical calculation suggest that the dual-emission of the AIDF luminogens likely results from the twisted intramolecular charge transfer transitions of the molecules,and the reversible mechanochromism properties probably stem from the interconversion of the quasi-axial and the quasi-equatorial conformations.

关键词： White-light emission Thermally activated delayed fluorescence Aggregation-induced delayed fluorescence mechanochromism Force-induced emission enhancement

Molecular Phylogeny Supports the Validity of Polypedates impresus Yang 2008

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Asian Herpetological Research 2013年第2期4卷 124-133页

作者： Shulin PAN Ningxin DANG Jishan WANG yitao zheng Dingqi RAO Jiatang LI School of Mining and Safety Engineering Yibin University College of Life Sciences Sichuan University Kunming Institute of Zoology Chinese Academy of Sciences Chengdu Institute of Biology Chinese Academy of Sciences

The taxonomy of the Asian tree frogs of the Polypedates leucomystax complex, a group of widespread and morphologically similar species, is very controversial. To ascertain the taxonomic status of these species, we investi- gated the historical relationships among representative samples based on - 2 kb of nucleotide sequences from the mito- chondrial 12S rRNA, tRNAvalinc, and 16S rRNA genes. Our phylogeny resolved five well supported lineages （A, B, C, D and E） in the P leucomystax complex. Polypedates impresus from Yunnan, China, Polypedates cf. mutus and Polypedates cf. megacephalus from Guangxi and Yunnan, China, and Laos constructed Clade C, which is monophyletic. In order to recognize the unique position of this clade, we considered P impresus in Clade C as a valid species. Following our phy- logeny, Chinese Polypedates, corresponding to the other four clades, should include four species： P. mutus （Clade A）; P. braueri （Clade B）; P impresus （Clade C） and P. megacephalus （Clade D）. P Ieucomystax （Clade E） is mainly distributed in the Malaysia, Indonesia and Philippines.

关键词： Chinese Polypedates nucleotide sequence Polypedates leucomystax complex taxonomy

Bile acid signaling in the regulation of whole body metabolic and immunological homeostasis

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Science China(Life Sciences) 2024年第5期67卷 865-878页

作者： Wei Jia yitao Li Kenneth C.P.Cheung Xiaojiao zheng School of Chinese Medicine Hong Kong Baptist UniversityHong KongChina Center for Translational Medicine and Shanghai Key Laboratory of Diabetes Mellitus Shanghai Sixth People's Hospital Affiliated to Shanghai JiaoTong University School of MedicineShanghai 200233China

Bile acids(BAs)play a crucial role in nutrient absorption and act as key regulators of lipid and glucose metabolism and immune homeostasis.Through the enterohepatic circulation,BAs are synthesized,metabolized,and reabsorbed,with a portion entering the vascular circulation and distributing systemically.This allows BAs to interact with receptors in all major organs,leading to organ-organ interactions that regulate both local and global metabolic processes,as well as the immune system.This review focuses on the whole-body effects of BA-mediated metabolic and immunological regulation,including in the brain,heart,liver,intestine,eyes,skin,adipose tissue,and muscle.Targeting BA synthesis and receptor signaling is a promising strategy for the development of novel therapies for various diseases throughout the body.

关键词： bile acid whole body metabolism immunity

Hydralazine represses Fpn ubiquitination to rescue injured neurons via competitive binding to UBA52

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Journal of Pharmaceutical Analysis 2024年第1期14卷 86-99页

作者： Shengyou Li Xue Gao Yi zheng Yujie Yang Jianbo Gao Dan Geng Lingli Guo Teng Ma Yiming Hao Bin Wei Liangliang Huang yitao Wei Bing Xia Zhuojing Luo Jinghui Huang Department of Orthopedics Xijing HospitalFourth Military Medical UniversityXi'an710032China

A major impedance to neuronal regeneration after peripheral nerve injury (PNI) is the activation of various programmed cell death mechanisms in the dorsal root ganglion. Ferroptosis is a form of programmed cell death distinguished by imbalance in iron and thiol metabolism, leading to lethal lipid peroxidation. However, the molecular mechanisms of ferroptosis in the context of PNI and nerve regeneration remain unclear. Ferroportin (Fpn), the only known mammalian nonheme iron export protein, plays a pivotal part in inhibiting ferroptosis by maintaining intracellular iron homeostasis. Here, we explored in vitro and in vivo the involvement of Fpn in neuronal ferroptosis. We first delineated that reactive oxygen species at the injury site induces neuronal ferroptosis by increasing intracellular iron via accelerated UBA52-driven ubiquitination and degradation of Fpn, and stimulation of lipid peroxidation. Early administration of the potent arterial vasodilator, hydralazine (HYD), decreases the ubiquitination of Fpn after PNI by binding to UBA52, leading to suppression of neuronal cell death and significant acceleration of axon regeneration and motor function recovery. HYD targeting of ferroptosis is a promising strategy for clinical management of PNI.

关键词： Ferroptosis UBA52 Ferroportin Ubiquitination Hydralazine Peripheral nerve injury

颈横动脉皮瓣在下咽癌喉功能保存术中的应用研究

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中国修复重建外科杂志 2022年第9期36卷 1144-1149页

作者：杨柳李文祝佼郑义涛魏波四川大学华西医院耳鼻咽喉头颈外科成都610041

目的探讨颈横动脉皮瓣在下咽癌喉功能保存术中的应用价值及局限性。方法2013年1月—2019年12月,收治18例下咽癌男性患者。患者年龄48~77岁,中位年龄65岁。病程3~8个月,平均5个月。术前经活检病理诊断为鳞状细胞癌。根据2017年第8版美国... 详细信息

目的探讨颈横动脉皮瓣在下咽癌喉功能保存术中的应用价值及局限性。方法2013年1月—2019年12月,收治18例下咽癌男性患者。患者年龄48~77岁,中位年龄65岁。病程3~8个月,平均5个月。术前经活检病理诊断为鳞状细胞癌。根据2017年第8版美国癌症联合委员会(AJCC)指南标准,TNM分期:T_(2)N_(0)M_(0)9例、T_(2)N_(1)M_(0)2例、T_(3)N_(0)M_(0)7例,cTNM分期:Ⅱ期9例、Ⅲ期9例。15例病变位于一侧下咽梨状窝,其中4例累及食道入口;3例病变位于下咽后壁,累及食道入口。采用气管切开+部分下咽-喉肿瘤切除+双侧颈淋巴结清扫术,术中切除下咽肿瘤及累及的部分喉组织后,以颈横动脉皮瓣修复缺损,皮瓣切取范围为4 cm×3 cm~6 cm×4 cm。皮瓣回流静脉为颈横动脉伴行静脉(7例)、颈外静脉(6例)及颈横动脉伴行静脉联合颈外静脉(5例)。术中2例皮瓣静脉回流不畅,行颈外静脉逆行回流锻炼以促进皮瓣经颈外静脉回流。供区切口直接缝合或作松弛切口缝合。术后3个月内均行放化疗治疗。采用带气囊气管套管防止患者术后早期误咽。结果手术时间4~6 h,平均4.5 h。患者均获随访,随访时间1~5年,平均2年6个月。术后病理检查示7例患侧颈淋巴结转移,颈Ⅴ区无淋巴结转移;余11例无淋巴结转移。术后16例皮瓣顺利成活;2例以颈外静脉回流的皮瓣被白色假膜覆盖,假膜脱落后未见皮瓣坏死。术后4例患者无明显误咽;14例出现明显误咽,其中13例3个月后明显减轻,1例6个月时仍误咽明显,拔除胃管后明显减轻。所有患者均无吸入性肺炎发生。1例术后1年2个月上纵膈淋巴结转移,1年3个月时死于肿瘤复发及肺部感染;余17例随访期间未见肿瘤复发或转移。7例患者于术后2~5个月顺利拔除气管套管,11例患者有不同程度误咽并经食道造影证实,保留气管套管。术后所有患者发音功能均保留。供区切口均Ⅰ期愈合,肩关节功能正常。结论对于颈Ⅴ区淋巴结无转移的下咽癌患者,采用颈横动脉皮瓣修复下咽-喉缺损可较好地保留喉功能;对于颈Ⅴ区淋巴结有可疑转移或颈横动脉伴行静脉发育不良患者,存在肿瘤复发或皮瓣坏死风险,该修复方法需谨慎使用。

关键词：颈横动脉皮瓣下咽癌喉功能修复重建

变轴力工程用水泥基复合材料RC组合柱抗震性能研究

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建筑结构 2023年第17期53卷 104-111页

作者：王永俊郑小刚郑易涛昭通市昭乐高速公路投资开发有限公司昭通657000 四川交大工程检测咨询有限公司成都611743

为探究变轴力对工程用水泥基复合材料RC组合柱(简称ECC-RC组合柱)抗震性能的影响,采用ABAQUS模拟恒定轴力作用下ECC-RC组合柱的拟静力试验,通过模拟与试验结果的对比分析,验证了有限元建模的准确性。基于此模型,建立19个ECC-RC组合柱以... 详细信息

为探究变轴力对工程用水泥基复合材料RC组合柱(简称ECC-RC组合柱)抗震性能的影响,采用ABAQUS模拟恒定轴力作用下ECC-RC组合柱的拟静力试验,通过模拟与试验结果的对比分析,验证了有限元建模的准确性。基于此模型,建立19个ECC-RC组合柱以轴力变化幅值与频率、剪跨比及纵筋直径为变量,以承载能力、变形性能及等效黏滞阻尼系数为表征手段,分析了ECC-RC组合柱的抗震性能。研究结果表明:变轴力作用下ECC-RC组合柱的滞回曲线与骨架曲线不再关于原点对称,正向加载时峰值荷载明显高于负向加载时峰值荷载的绝对值,但正向加载时刚度退化更为显著。相比于恒定轴力,变轴力作用下ECC-RC组合柱的屈服位移、极限位移、延性系数及等效黏滞阻尼系数均显著降低。此外,当轴力变化频率与水平位移加载频率不一致时,变轴力会影响延性系数与等效黏滞阻尼系数随剪跨比的变化规律。

关键词： ECC-RC组合柱变轴力抗震性能 ABAQUS

正负气压组合滚轮式油菜精密排种器设计与试验

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农业机械学报 2024年第5期55卷 63-76页

作者：廖宜涛刘嘉诚廖庆喜郑娟李甜蒋杉华中农业大学工学院武汉430070 农业农村部长江中下游农业装备重点实验室武汉430070

针对油菜种子粒径小、质量轻,单粒排种难度较大的问题,设计了一种正负气压组合滚轮式精密排种器。阐明了排种器工作原理,开展排种器吸种、携种和卸种环节受力分析和排种滚轮对种群拖带过程解析;提出了通过控制充种区种层高度和种群压力... 详细信息

针对油菜种子粒径小、质量轻,单粒排种难度较大的问题,设计了一种正负气压组合滚轮式精密排种器。阐明了排种器工作原理,开展排种器吸种、携种和卸种环节受力分析和排种滚轮对种群拖带过程解析;提出了通过控制充种区种层高度和种群压力的防拖带堆积机理,设计了一种侧向充种、拖带种子自由回落的充种室结构,利用离散元仿真研究了充种种层高度和充种室结构对排种器充种区内充种性能的影响及对种群拖带堆积的解决情况;仿真结果表明,排种器内种子随着充种种层高度的增大,种群平均动能均值逐渐增大,对种群平均扰动能力逐渐增强;在充种种层高度50 mm条件下,设计的防拖带堆积充种室降低了充种区域底部种群所受的压力,未出现种群拖带堆积现象,且保持了对充种区域种群的扰动作用。在JPS-12型排种器检测试验台上进行了排种器性能试验,结果表明,当排种转速为15~30 r/min、吸种负压为1.0~1.2 kPa时,排种器合格指数均保持在90%以上;设计装配正负气压组合滚轮式精密排种器的播种机开展播种试验,田间实测出苗后株距稳定性变异系数为4.4%,各行苗数一致性变异系数为8.14%;研究结果表明设计的排种器满足精密播种要求。

关键词：油菜精密排种器正负气压组合滚轮式