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The Saffold Virus-Penang 2B and 3C Proteins, but not the L Protein,Induce Apoptosis in HEp-2 and Vero Cells

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Virologica Sinica 2019年第3期34卷 262-269页

作者： yishi xu Carla Bianca Luena Victorio Tao Meng Qiang Jia Yee-Joo Tan Kaw Bing Chua Temasek Life Sciences Laboratory 1 Research LinkNational University of SingaporeSingapore 117604Singapore Department of Microbiology and Immunology Yong Loo Lin School of MedicineNational University Health SystemNational University of SingaporeSingapore 117545Singapore Department of Biological Sciences National University of SingaporeSingapore 117543Singapore Institute of Molecular and Cell Biology A*STAR (Agency for ScienceTechnology and Research)Singapore 138673Singapore

Our previous work has shown that Saffold virus(SAFV)induced several rodent and primate cell lines to undergo apoptosis(xu et *** Emerg Microb Infect 3:1-8,2014),but the essential viral proteins of SAFV involved in apoptotic activity lack *** this study,we individually transfected the viral proteins of SAFV into HEp-2 and Vero cells to assess their ability to induce apoptosis,and found that the 2B and 3C proteins are *** investigation indicated the trans-membrane domain of the 2B protein is essential for the apoptotic activity and tetramer formation of the 2B *** research provides clues for the possible mechanisms of apoptosis induced by SAFV in different cell *** also opens up new directions to study viral proteins(the 2B,3C protein),and sets the stage for future exploration of any possible link between SAFV,inclusive of its related uncultivable genotypes,and multiple sclerosis.

关键词： Saffold virus(SAFV)Apoptosis Viral proteins

Reverse Genetic Analysis of Adaptive Mutations within the Capsid Proteins of Enterovirus 71(EV-A71) Strains Necessary for Infection of CHO-K1 Cells

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Virologica Sinica 2020年第1期35卷 110-114页

作者： Carla Bianca Luena Victorio yishi xu Qimei Ng Vincent T.K.Chow Kaw Bing Chua Temasek Lifesciences Laboratory 1 Research LinkNational University of SingaporeSingapore 117604Singapore Host and Pathogen Interactivity Laboratory Department of MicrobiologyYong Loo Lin School of MedicineNational University Health SystemNational University of SingaporeSingapore 117545Singapore Present Address:Laboratory for Translational and Molecular Imaging Cancer and Stem Cell Biology ProgrammeDuke-NUS Medical SchoolSingapore 169857Singapore

Dear Editor,Previous studies had described the adaptation of enterovirus 71(EV-A71)strains that enabled entry and viral replication in Chinese Hamster Ovary(CHO)cell line(Zaini and McMinn 2012;Zaini et al.2012).These adapted strains derived from serial passage of a clinical isolate in CHO cells exhibited an amino acid substitution at VP2149,which enhanced viral replication by 100*1000-fold compared to the clinical *** VP2149 mutation was claimed responsible for adaptation to CHO-K1 cells without performing detailed molecular analyses to support these *** this study,we evaluate various VP1 and VP2 mutations in two CHO-adapted EV-A71 strains derived in our lab to assess their contribution to the phenotype of CHO cell adaptation.

关键词： adapted passage claimed

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雨淋灭火系统优化布置探讨

给水排水 2010年第8期36卷 85-87页

作者：徐以时深圳市建筑设计研究总院有限公司深圳518031

通过优化雨淋系统中每个雨淋阀控制的喷水面积大小及位置分割,在满足国家相关规范和保障安全的同时减小雨淋系统的设计流量、消防水池容积,从而减少工程造价。以长条形及品字形舞台设计平面为例,比较了不同喷水区域划分的总设计流量、... 详细信息

通过优化雨淋系统中每个雨淋阀控制的喷水面积大小及位置分割,在满足国家相关规范和保障安全的同时减小雨淋系统的设计流量、消防水池容积,从而减少工程造价。以长条形及品字形舞台设计平面为例,比较了不同喷水区域划分的总设计流量、消防水池容积、设备造价,认为根据不同平面形式采用适当增加雨淋阀并合理分割每个雨淋阀控制区域的系统更具优势。

关键词：雨淋系统雨淋阀喷水面积设计流量消防水池容积舞台

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起吊眼板的结构非线性响应分析

哈尔滨工程大学学报 2023年第1期44卷 117-124页

作者：刘宁陈乾刘大顺张路阳许一石哈尔滨工程大学船舶工程学院黑龙江哈尔滨150001

气垫船船体起吊主要通过起吊眼板来实现,眼板结构直接关系到船体建造与运输过程中的可靠性与安全性。起吊过程中,销轴与眼板之间属于接触问题,是一种典型的边界条件非线性问题,为了研究其结构受力情况,本文讨论了多种对眼板强度校核的... 详细信息

气垫船船体起吊主要通过起吊眼板来实现,眼板结构直接关系到船体建造与运输过程中的可靠性与安全性。起吊过程中,销轴与眼板之间属于接触问题,是一种典型的边界条件非线性问题,为了研究其结构受力情况,本文讨论了多种对眼板强度校核的理论计算方法,并以加敷板的C型铝合金眼板为例用LS-DYNA有限元软件对起吊接触问题进行了模拟仿真,在考虑销轴是否变形2种情况下,分析校核了不同起吊速度下眼板结构强度,并对仿真结果进行了对比分析。同时考虑材料的非线性,采用塑性极限方法对眼板的进行极限载荷分析,得到眼板塑性极限承载能力,并定义得到极限载荷系数,以保证眼板使用的安全可靠性。上述研究可以为该形式下铝合金起吊眼板的实际应用提供切实可行的理论指导性意见。

关键词：起吊眼板边界非线性有限元分析塑性极限分析铝合金气垫船强度校核极限载荷

基于飞秒激光诱导双折射效应的时间胶囊的制备(特邀)

同方期刊数据库博看期刊评论

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光子学报 2021年第6期50卷 58-65页

作者：王琳樊华李臻赜王熠胥亦实王磊陈岐岱吉林大学电子科学与工程学院长春130012 清华大学精密仪器系北京100000

利用双折射显微镜和光学显微镜研究了飞秒激光石英材料内部诱导的双折射效应。结果表明,双折射的光学延迟量随脉冲数的增加而增加,并出现两种双折射结构。其中Type X结构需要至少20个脉冲,单脉冲能量不高于854 nJ,脉冲宽度不低于300 fs... 详细信息

利用双折射显微镜和光学显微镜研究了飞秒激光石英材料内部诱导的双折射效应。结果表明,双折射的光学延迟量随脉冲数的增加而增加,并出现两种双折射结构。其中Type X结构需要至少20个脉冲,单脉冲能量不高于854 nJ,脉冲宽度不低于300 fs且不高于600 fs。此外,以"光子学报"等汉字为例,阐述了激光诱导双折射的数据存储过程,制备了包涵吉林省省史省情、吉林大学校史、清华大学校史的数据存储时间胶囊样品。

关键词：超快激光光存储超快激光加工双折射效应数据存储

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飞秒激光永久光存储的发展及挑战

中国激光 2022年第10期49卷 152-168页

作者：王磊张栩王熠王琳王晴臧雪李瑞凡胥亦实李臻赜陈岐岱吉林大学电子科学与工程学院集成光电子学国家重点实验室吉林长春130012 清华大学精密仪器系精密测试技术与仪器国家重点实验室北京100084 吉林大学材料科学与工程学院吉林长春130012

面向大数据存储,总结当前冷数据存储的主要方式及特点,针对长寿命和高容量的需求,介绍飞秒激光永久光存储的概念和基本存储内涵;围绕透明介质材料体内改性的类型,依次介绍三维光存储和五维光存储的历史发展过程;阐述了当前具有双折射特... 详细信息

面向大数据存储,总结当前冷数据存储的主要方式及特点,针对长寿命和高容量的需求,介绍飞秒激光永久光存储的概念和基本存储内涵;围绕透明介质材料体内改性的类型,依次介绍三维光存储和五维光存储的历史发展过程;阐述了当前具有双折射特性的存储单元形成机制,超百层的高密度存储技术,225 kB/s单通道、潜在MB/s多通道的快速直写机制;并从纳米区域的电场连续性边界条件和光学衍射极限出发,展望飞秒激光永久光存储在存储容量和写入速度方面的挑战。

关键词：光电器件加工飞秒激光激光材料加工光与物质相互作用光存储纳米光栅

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图案化聚合物表面的形状记忆特性

吉林大学学报（理学版） 2017年第3期55卷 702-706页

作者：关薇胥亦实牛立刚王艳辉于颜豪夏虹吉林大学电子科学与工程学院集成光电子学国家重点联合实验室长春130012

利用纳秒激光双光束干涉的光刻方法,在光刻胶层制备具有周期性的一维光栅结构样品,并研究其形状记忆特性.结果表明:显影后的样品在环境温度为195℃及压力的作用下,光栅形貌发生改变,光栅垂直高度降低,衍射光强减弱;当样品冷却至室温时,... 详细信息

利用纳秒激光双光束干涉的光刻方法,在光刻胶层制备具有周期性的一维光栅结构样品,并研究其形状记忆特性.结果表明:显影后的样品在环境温度为195℃及压力的作用下,光栅形貌发生改变,光栅垂直高度降低,衍射光强减弱;当样品冷却至室温时,光栅形貌可维持不变;当样品的环境温度为120℃时,光栅形貌恢复原状,实现微观形状记忆功能.

关键词：微加工双光束干涉形状记忆聚合物微图案化

Effectiveness and safety of red yeast rice predominated by monacolin Kβ-hydroxy acid form for hyperlipidemia treatment and management

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Journal of Traditional Chinese Medicine 2022年第2期42卷 264-271页

作者： CHEN Benjian HUANG Xiaodan PENG Huiting LI yishi CAO Yongtao WU Huanlin xu Danping Department of Medical Guangdong Provincial Hospital of Chinese MedicineThe Second Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhou 510120China the Second Clinical College Guangzhou University of Chinese MedicineGuangzhou 510405China the First Clinical College Guangzhou University of Chinese MedicineGuangzhou 510405China Department of Mathematical&Computer Sciences Indiana University of PennsylvaniaIndiana 15705PAUSA Department of Cardiology Dongzhimen HospitalBeijing University of Chinese MedicineBeijing 350122China Department of Traditional Chinese Medicine the Eighth Affiliated HospitalSunYat-Sen UniversityShenzhen 518033China

OBJECTIVE:To assess the lipid-lowering activity and safety of a dietary supplement containing monacolin Kβ-hydroxy acid form(MKA),Heye(Folium Nelumbinis),and Cangzhu(Rhizoma Atractylodis Lanceae),compared to lifestyle ***:Totally 117 subjects with moderate to severe dyslipidemia(according to Chinese guidelines)and low CV risk were randomly assigned into three treatment groups:lifestyle modification(LM),LM plus a low dosage of MKA,LM plus a high dosage of MKA,and treated for 60 *** primary endpoint was the reduction of low density lipoprotein cholesterol(LDL-C)and total cholesterol(TC).Safeties along with Traditional Chinese Medicine Syndromes were assessed through the ***:A low dosage of MKA along with lifestyle modifications caused a significant decrease in LDL-C by 15.6%on average(95%CI,9.6%to 21%)with,a decrease in TC by 15.3%on average(95%CI,9.26%to 21.4%),and a decrease in non-HDL-C by 35.4%(95%CI,25.76%to 41.34%).Weak evidence of a reduction of triglycerides but an increment of HDL-C was observed in patients with severe *** severe adverse events occurred during the ***:Our results confirm the LDL-C and TC lowering properties of MKA is clinically *** also produces a significant reduction of non-HDL-C,and slightly effects on TG and HDL-C as well.

关键词： hydroxy acids lovastatin hyperlipidemias cholesterol cardiovascular prevention lifestyle modifications red yeast rice

Targeted Mutagenesis of Duplicated Genes in Caenorhabditis elegans Using CRISPR-Cas9

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Journal of Genetics and Genomics 2016年第2期43卷 103-106页

作者： Suhong xu Zhiping Wang Kyung Won Kim yishi Jin Andrew D.Chisholm Division of Biological Sciences Section of Cell and Developmental BiologyUniversity of CaliforniaSan Diego9500 Gilman DriveLa JollaCA 92093USA Division of Biological Sciences Section of NeurobiologyUniversity of CaliforniaSan Diego9500 Gilman DriveLa JollaCA 92093USA Howard Hughes Medical Institute University of CaliforniaSan DiegoLa JollaCA 92093USA School of Medicine Zhejiang UniversityHangzhouChina

CRISPR （clustered regularly interspaced short palindromic repeats）-Cas9-based genome editing has revolutionized func- tional genomics in many biological research fields. The specificity and potency of CR1SPR-Cas9 genome editing make it ideal for investigating the function of genes in vivo （Hsu et al., 2014）. Gene duplication is a key driver of evolu- tionary novelty （Taylor and Raes, 2004）. However, duplicated genes with near-identical sequences and functional redun- dancy have posed challenges for genetic analysis （Woollard, 2005）. The functions of duplicated genes can be assessed by simultaneous knockdown using homology-based methods such as RNA interference （RNAi）（Tischler et al., 2006）, Generation of double or triple mutants is an alternative way to assess functional redundancy of duplicated genes, However, generation of such compound mutants by forward or reverse genetic methods is time consuming.

关键词： gene Targeted Mutagenesis of Duplicated Genes in Caenorhabditis elegans Using CRISPR-Cas9