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Gynoecy instability in cucumber(Cucumis sativus L.)is due to unequal crossover at the copy number variation-dependent Femaleness(F)locus

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Horticulture Research 2020年第1期7卷 2342-2356页

作者： Zheng Li Yonghua Han Huanhuan Niu Yuhui Wang Biao Jiang yiqun weng Horticulture Department University of WisconsinMadisonWI 53706USA College of Horticulture Northwest A&F UniversityYanglingShaanxi 712100China Institute of Integrative Plant Biology School of Life SciencesJiangsu Normal UniversityXuzhou 221116China Vegetable Research Institute Guangdong Academy of Agricultural ScienceGuangzhouGuangdong 510640China USDA-ARS Vegetable Crops Research UnitMadisonWI 53706USA

Cucumber,Cucumis sativus is an important vegetable crop,and gynoecy has played a critical role in yield increase of hybrid cucumber *** has a unique genetic system for gynoecious sex expression,which is determined by the copy number variation(CNV)-based,dominant,and dosage-dependent femaleness(F)***,this gynoecy expression system seems unstable since monecious plants could often be found in Fdependent gynoecious cucumber *** hypothesized that gynoecy instability(gynoecy loss)may be due to unequal crossing over(UCO)during meiosis among repeat units of the *** this study,using high throughput genome resequencing,fiber-FISH and genomic qPCR analyses,we first confirmed and refined the structure of the F locus,which was a CNV of a 30.2-kb tandem *** plants contained three genes:CsACS1,CsACS1G,and CsMYB,of which CsACS1G is a duplication of CsACS1 but with a recombinant distal promoter that may contribute to gynoecy sex *** two large populations from self-pollinated gynoecious inbred lines,‘gynoecy loss’mutants were identified with similar mutation rates(~0.12%).We show that these monecious mutants have lost *** addition,we identified gynoecious lines in natural populations that carry two copies of *** proposed a model to explain gynoecy instability in F-dependent cucumbers,which is caused by UCO among CSACS1/G units during *** findings present a convincing case that the phenotypic variation of an economically important trait is associated with the dynamic changes of copy numbers at the F *** work also has important implications in cucumber breeding.

关键词： fir number dosage

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Horticulture Research 2020年第1期7卷 434-448页

作者： Hu Wang Huanhuan Niu Chuang Li Guoyan Shen Xiaofeng Liu yiqun weng Tao Wu Zheng Li State Key Laboratory of Crop Stress Biology in Arid Areas College of HorticultureNorthwest A&F UniversityYanglingShaanxi 712100China Department of Vegetable Sciences Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable CropsChina Agricultural UniversityBeijing 100193China USDA-ARS Vegetable Crops Research UnitHorticulture DepartmentUniversity of WisconsinMadisonWI 53706USA College of Horticulture and Landscape Architecture Hunan Agricultural UniversityChangshaHunan 410128China

In plants,WUSCHEL-related homeobox1(WOX1)homologs promote lamina mediolateral ***,the downstream components linking WOX1 and lamina development remain *** this study,we revealed the roles of WOX1 in palmate leaf expansion in cucumber(Cucumis sativus).A cucumber mango fruit(mf)mutant,resulting from truncation of a WOX1-type protein(CsWOX1),displayed abnormal lamina growth and defects in the development of secondary and smaller ***1 was expressed in the middle mesophyll and leaf margins and rescued defects of the Arabidopsis wox1 prs double *** analysis revealed that genes involved in auxin polar transport and auxin response were highly associated with leaf *** of the cucumber mf rl(round leaf)double mutant revealed that CsWOX1 functioned in vein development via PINOID(CsPID1)-controlled auxin *** of CsWOX1 in cucumber(CsWOX1-OE)affected vein patterning and produced‘butterfly-shaped’***1 physically interacted with CsTCP4a,which may account for the abnormal lamina development in the mf mutant line and the smaller leaves in the CsWOX1-OE *** findings demonstrated that CsWOX1 regulates cucumber leaf vein development by modulating auxin polar transport;moreover,CsWOX1 regulates leaf size by controlling CIN-TCP genes.

关键词： size WUS shaped

A Sequencing-Based Linkage Map of Cucumber

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Molecular Plant 2015年第6期8卷 961-963页

作者： Zhou, Qian Miao, Han Li, Shuai Zhang, Shengping Wang, Ye weng, yiqun Zhang, Zhonghua Huang, Sanwen Gu, Xingfang Chinese Acad Agr Sci Key Lab Biol & Genet Improvement Hort Crops Inst Vegetables & Flowers Minist AgrSino Dutch Joint Lab Hort Gen Beijing 10081 Peoples R China Chinese Acad Agr Sci Agr Genom Inst Shenzhen Shenzhen 518120 Peoples R China Univ Wisconsin Vegetable Crops Res Unit Dept Hort USDAARS Madison WI 53706 USA

Cucumber （Cucumis sativus L., 2n = 2x = 14） is an economically important vegetable worldwide and has served as a model sys- tem for plant vascular biology and sex determination studies. We reported the draft genome assembly of the North China type cucumber line 9930 in 2009 （Huang et al., 2009a） and constructed a single-base resolution map of cucumber genomic variation by resequencing of the genomes of 115 core germplasm lines （Qi et al., 2013）, which enabled genome-wide association studies of important traits in cucumber （Shang et al., 2014）.

关键词： CUCUMBERS -- Genetics PLANT genomes PLANT reproduction GENETIC aspects

Efficient Transposition of the Retrotransposon Tnt1 in Cucumber(Cucumis sativus L.)

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Horticultural Plant Journal 2018年第3期4卷 111-116页

作者： ZHANG Qi DU Hui LV Duo XIAO Tingting PAN Jian HE Huanle WANG Gang CAI Run weng yiqun PAN Junsong Plant Science Department School of Agriculture and BiologyShanghai Jiao Tong University USDA-ARS Vegetable crops Research UnitHorticulture DepartmentUniversity of Wisconsin

Tnt1 is an active retrotransposon originally identified in tobacco(Nicotiana tabacum L.)(Grandbastien et al.,1989),but its transposition activity could be activated through tissue culture in other plant *** insertions are stable and inheritable in the progeny,which has made it a valuable and versatile tool for developing insertional mutagenesis libraries in several plant ***,we explored its utility for mutagenesis in cucumber(Cucumis sativus L.).T_3 Tnt1 transgenic cucumber plants were subjected to tissue culture to regenerate self-pollinated *** PCR and analyses and Southern hybridization,we found regenerated plants maintained the original Tnt1 insertion and created new insertions suggesting characteristic re-transposition activity of Tnt1 during this *** genome walking,some flanking sequences of Tnt1 insertions were recovered in regenerated *** results demonstrated that Tnt1 could be stably inherited and re-transposable during tissue culture in cucumber and that it is feasible to use for developing an insertional mutagenesis library for cucumber.

关键词： cucumber Tnt1 retrotransposon tissue culture mutagenesis library

Melon short internode(CmSi)encodes an ERECTA-like receptor kinase regulating stem elongation through auxin signaling

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Horticulture Research 2020年第1期7卷 269-282页

作者： Sen Yang Kaige Zhang Huayu Zhu Xiaojing Zhang Wenkai Yan Nana Xu Dongming Liu Jianbin Hu Yufeng Wu yiqun weng Luming Yang College of Horticulture Henan Agricultural University63 Nongye Road450002 ZhengzhouChina State Key Laboratory of Crop Genetics and Germplasm Enhancement Bioinformatics CenterNanjing Agricultural University210095 NanjingChina USDA-ARS Vegetable Crops Research UnitHorticulture DepartmentUniversity of Wisconsin–MadisonMadisonWI 53706USA

Plant height is one of the most important agronomic traits that directly determines plant architecture,and compact or dwarf plants can allow for increased planting density and land utilization as well as increased lodging resistance and economic *** least four dwarf/semidwarf genes have been identified in different melon varieties,but none of them have been cloned,and little is known about the molecular mechanisms underlying internode elongation in ***,we report map-based cloning and functional characterization of the first semidwarf gene short internode(Cmsi)in melon,which encodes an ERECTA-like receptor kinase regulating internode ***-temporal expression analyses revealed that CmSI exhibited high expression in the vascular bundle of the main stem during internode *** expression level of CmSI was positively correlated with stem length in the different melon varieties *** expression of CmSI in Arabidopsis and cucumber suggested CmSI as a positive regulator of internode elongation in both *** quantitation and transcriptome analysis showed that the auxin content and the expression levels of a number of genes involved in the auxin signaling pathway were altered in the semidwarf mutant,including several well-known auxin transporters,such as members of the ABCB family and PINFORMED genes.A melon polar auxin transport protein CmPIN2 was identified by protein–protein interaction assay as physically interacting with CmSI to modulate auxin ***,CmSI functions in an auxin-dependent regulatory pathway to control internode elongation in *** findings revealed that the ERECTA family gene CmSI regulates stem elongation in melon through auxin signaling,which can directly affect polar auxin transport.

关键词： species dwarf traits

Comprehensive analysis of NAC transcription factors and their expression during fruit spine development in cucumber (Cucumis sativus L.)

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Horticulture Research 2018年第1期5卷 556-569页

作者： Xingwang Liu Ting wang Ezra Bartholomew Kezia Black Mingming Dong Yaqi Zhang Sen Yang Yanling Cai Shudan Xue yiqun weng Huazhong Ren Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops College of HorticultureChina Agricultural University100193BeijingP.R.China Department of Horticulture USDA-ARSVegetable Crops Research UnitUniversity of Wisconsin-MadisonWI53706USA

The cucumber(Cucumis sativus L.)is an important vegetable crop worldwide,and fruit trichomes or spines are an important trait for external fruit *** mechanisms underlying spine formation are not well understood,but the plant-specific NAC family of transcription factors may play important roles in fruit spine initiation and *** this study,we conducted a genome-wide survey and identified 91 NAC gene homologs in the cucumber *** analysis classified these genes into six subfamilies;each contained a varying number of NAC family members with a similar intron–exon structure and conserved *** real-time PCR analysis revealed tissue-specific expression patterns of these genes,including 10 and 12 that exhibited preferential expression in the stem and fruit,*** of the 91 NAC genes showed higher expression in the wild-type plant than in its near-isogenic trichome mutant,suggesting their important roles in fruit spine *** application of four plant hormones promoted spine formation and increased spine density on the cucumber fruits;several NAC genes showed differential expression over time in response to phytohormone treatments on cucumber fruit,implying their essential roles in fruit-trichome *** the NAC genes identified,12 were found to be targets of 13 known cucumber ***,these findings provide a useful resource for further analysis of the interactions between NAC genes and genes underlying trichome organogenesis and development during fruit spine development in cucumber.

关键词： development. analysis spine

Molecularly tagged genes and quantitative trait loci in cucumber with recommendations for QTL nomenclature

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Horticulture Research 2020年第1期7卷 2678-2697页

作者： Yuhui Wang Kailiang Bo Xingfang Gu Junsong Pan Yuhong Li Jinfeng Chen Changlong Wen Zhonghai Ren Huazhong Ren Xuehao Chen Rebecca Grumet yiqun weng Department of Horticulture University of WisconsinMadisonWI 53706USA Institute of Vegetables and Flowers Chinese Academy of Agricultural SciencesBeijing 100081China Department of Plant Sciences Shanghai Jiaotong UniversityShanghai 200240China Horticulture College Northwest A&F UniversityYangling 712100China Horticulture College Nanjing Agricultural UniversityNanjing 210095China Beijing Vegetable Research Center Beijing Academy of Agricultural and Forestry SciencesBeijing 100097China College of Horticulture Science and Engineering Shandong Agricultural UniversityTai’an 271018China College of Horticulture China Agricultural UniversityBeijing 100193China College of Horticulture and Plant Protection Yangzhou UniversityYangzhou 225009China Department of Horticulture Michigan State UniversityEast LansingMI 48824USA USDAARS Vegetable Crops Research Unit 1575 Linden Dr.MadisonWI 53706USA

Cucumber,Cucumis sativus L.(2n=2x=14),is an important vegetable crop *** was the first specialty crop with a publicly available draft *** relatively small,diploid genome,short life cycle,and selfcompatible mating system offers advantages for genetic *** recent years,significant progress has been made in molecular mapping,and identification of genes and QTL responsible for key phenotypic traits,but a systematic review of the work is ***,we conducted an extensive literature review on mutants,genes and QTL that have been molecularly mapped or characterized in *** documented 81 simply inherited trait genes or major-effect QTL that have been cloned or fine *** each gene,detailed information was compiled including chromosome locations,allelic variants and associated polymorphisms,predicted functions,and diagnostic markers that could be used for marker-assisted selection in cucumber *** also documented 322 QTL for 42 quantitative traits,including 109 for disease resistances against seven *** alignment of these QTL on the latest version of cucumber draft genomes,consensus QTL across multiple studies were inferred,which provided insights into heritable correlations among different *** collaborative efforts among public and private cucumber researchers,we identified 130 quantitative traits and developed a set of recommendations for QTL nomenclature in *** is the first attempt to systematically summarize,analyze and inventory cucumber mutants,cloned or mapped genes and QTL,which should be a useful resource for the cucurbit research community.

关键词： trait traits cucumber

The USDA cucumber (Cucumis sativus L.) collection: genetic diversity, population structure, genome-wide association studies, and core collection development

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Horticulture Research 2018年第1期5卷 236-248页

作者： Xin Wang Kan Bao Umesh K.Reddy Yang Bai Sue A.Hammar Chen Jiao Todd C.Wehner Axel O.Ramírez-Madera yiqun weng Rebecca Grumet Zhangjun Fei Boyce Thompson Institute Cornell UniversityIthacaNY14853USA Gus R.Douglass Institute and Department of Biology West Virginia State UniversityInstituteVirginiaWV25112USA Department of Horticulture Michigan State UniversityEast LansingMI48824USA Horticultural Science Department North Carolina State UniversityRaleighNC27695USA Horticulture Department University of WisconsinMadisonWI53706USA USDA-ARS Vegetable Crops Research Unit MadisonWI53706USA USDA-ARS Robert W.Holley Center for Agriculture and Health IthacaNY14853USA

Germplasm collections are a crucial resource to conserve natural genetic diversity and provide a source of novel traits essential for sustained crop *** collection,preservation and utilization of these materials depends upon knowledge of the genetic variation present within the *** we use the high-throughput genotyping-by-sequencing(GBS)technology to characterize the United States National Plant Germplasm System(NPGS)collection of cucumber(Cucumis sativus L.).The GBS data,derived from 1234 cucumber accessions,provided more than 23 K high-quality single-nucleotide polymorphisms(SNPs)that are well distributed at high density in the genome(~1 SNP/10.6 kb).The SNP markers were used to characterize genetic diversity,population structure,phylogenetic relationships,linkage disequilibrium,and population differentiation of the NPGS cucumber *** results,providing detailed genetic analysis of the *** collection,complement NPGS descriptive information regarding geographic origin and phenotypic *** also identified genome regions significantly associated with 13 horticulturally important traits through genome-wide association studies(GWAS).Finally,we developed a molecularly informed,publicly accessible core collection of 395 accessions that represents at least 96%of the genetic variation present in the ***,the information obtained from the GBS data enabled deep insight into the diversity present and genetic relationships among accessions within the collection,and will provide a valuable resource for genetic analyses,gene discovery,crop improvement,and germplasm preservation.

关键词： sustained collection structure

Chromosome-specific painting and pachytene mapping of homoeologous chromosome pairing in Cucumis species using bulked oligonucleotides

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Chromosome-specific painting and pachytene mapping of homoeo...