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Apple SERRATE negatively mediates drought resistance by regulating MdMYB88 and MdMYB124 and microRNA biogenesis

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Horticulture Research 2020年第1期7卷 1388-1398页

作者： Xuewei Li Pengxiang Chen yinpeng xie Yan Yan Liping Wang Huan Dang Jing Zhang Lingfei Xu Fengwang Ma Qingmei Guan State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple College of HorticultureNorthwest A&F UniversityYangling712100 ShaanxiPR China

The function of serrate(SE)in miRNA biogenesis in Arabidopsis is well elucidated,whereas its role in plant drought resistance is largely *** this study,we report that MdSE acts as a negative regulator of apple(Malus×domestica)drought resistance by regulating the expression levels of MdMYB88 and MdMYB124 and miRNAs,including mdm-miR156,mdm-miR166,mdm-miR172,mdm-miR319,and *** interacts with MdMYB88 and MdMYB124,two positive regulators of apple drought *** decreases the transcript and protein levels of MdMYB88 and MdMYB124,which directly regulate the expression of MdNCED3,a key enzyme in abscisic acid(ABA)***,MdSE is enriched in the same region of the MdNECD3 promoter where MdMYB88/MdMYB124 ***,MdSE RNAi transgenic plants are more sensitive to ABA-induced stomatal closure,whereas MdSE OE plants are less *** addition,under drought stress,MdSE is responsible for the biogenesis of mdm-miR399,a negative regulator of drought resistance,and negatively regulates miRNAs,including mdm-miR156,mdm-miR166,mdm-miR172,and mdm-miR319,which are positive regulators of drought *** together,by revealing the negative role of MdSE,our results broaden our understanding of the apple drought response and provide a candidate gene for apple drought improvement through molecular breeding.

关键词： drought resistance RATE

Regulation of phenylpropanoid biosynthesis by MdMYB88 and MdMYB124 contributes to pathogen and drought resistance in apple

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Horticulture Research 2020年第1期7卷 1525-1535页

作者： Dali Geng Xiaoxia Shen yinpeng xie Yusen Yang Ruiling Bian Yuqi Gao Pengmin Li Liying Sun Hao Feng Fengwang Ma Qingmei Guan Laboratory of Apple College of HorticultureNorthwest A&F UniversityYanglingShaanxi 712100China State Key Laboratory of Crop Stress Biology for Arid Areas College of Plant ProtectionNorthwest A&F UniversityYanglingShaanxi 712100China

MdMYB88 and MdMYB124 have been demonstrated to be responsible for lignin accumulation in apple under drought *** this study,using a metabolomic approach,we identified differentially accumulated phenylpropanoid and flavonoid metabolites in MdMYB88/124 transgenic RNAi plants under control and long-term drought stress conditions in apple *** confirmed the regulation of phenylalanine by MdMYB88 and MdMYB124 via UPLC-MS in apple roots under both control and drought *** Electrophoretic Mobility Shift Assay(EMSA)and ChIPquantitative PCR(qPCR)analyses,we found that MdMYB88 positively regulates the MdCM2 gene,which is responsible for phenylalanine biosynthesis,through binding to its promoter *** long-term drought conditions,MdMYB88/124 RNAi plants consistently accumulated increased amounts of H2O2 and MDA,while MdMYB88 and MdMYB124 overexpression plants accumulated decreased amounts of H2O2 and *** also examined the accumulation of metabolites in the phenylpropanoid biosynthesis pathway in the leaves of MdMYB88 and MdMYB124 transgenic apple plants after long-term drought *** found that metabolites responsible for plant defense,including phenylpropanoids and flavonoids,accumulated less in the RNAi plants but more in the overexpression plants under both control and drought *** further demonstrated that MdMYB88/124 RNAi plants were more sensitive to Alternaria alternata *** and Valsa mali,two pathogens that currently severely threaten apple *** contrast,MdMYB88 and MdMYB124 overexpression plants were more tolerant to these *** cumulative results of this study provided evidence for secondary metabolite regulation by MdMYB88 and MdMYB124,further explained the molecular roles of MdMYB88 and MdMYB124 in drought resistance,and provided information concerning molecular aspects of their roles in disease resistance.

关键词： drought resistance roots

An R2R3 MYB transcription factor is critical for cold stress tolerance in apple

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An R2R3 MYB transcription factor is critical for cold stress...

Genome-wide identification of droughtresponsive microRNAs in two sets of Malus from interspecific hybrid progenies

2016年全国植物生物学大会

作者： yinpeng xie Pengxiang Chen Liping Wang Yan Yan Haiyan Li Xuewei Li Jidi Xu Fengwang Ma Qingmei Guan College of Horticulture Northwest A&F University State Key Laboratory of Crop Stress Biology in Arid Areas Northwest A&F University

Cold stress is one of the adverse effects on apple distribution and production. However,the molecular mechanisms underlying apple cold stress tolerance are not clear. In this study,we cloned a novel R2R3 MYB transcription factor,Md MYB5523,and its close homolog Md MYB0970 from Golden Delicious(Malus × domestica). Expression of Md MYB5523 and Md MYB0970 was slightly induced by cold stress. Sub-cellular localization revealed that both proteins are localized in the nucleus. With Affinity Purification-Mass Spectrometry(AP-MASS) and yeast two-hybrid screening,we obtained two interacting partners of Md MYB5523 and Md MYB0970,Md HYL1(HYPONASTIC LEAVES 1) and Md SERRATE. Chromatin Immunoprecipitation-PCR showed that Md MYB5523 and Md MYB0970 target genes involved in cold stress,including Md SIZ1 and Md CSP3(COLD SHOCK DOMAIN PROTEIN 3). RNAi lines of Md MYB5523 and Md MYB0970 in Gala-3(Malus × domestica) showed increased sensitivity to cold stress,while over-expression lines of Md MYB5523 or Md MYB0970 showed increased tolerance to cold stress,indicating that Md MYB5523 and Md MYB0970 are two positive regulators of cold stress in apple. Taken together,our results indicate that Md MYB5523 and Md MYB0970 played important roles in cold stress tolerance of apple.

关键词： Malus × domestica Md MYB5523 and Md MYB0970 cold stress

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Horticulture Research 2019年第1期6卷 794-811页

作者： Chundong Niu Haiyan Li Lijuan Jiang Mingjia Yan Cuiying Li Dali Geng yinpeng xie Yan Yan Xiaoxia Shen Pengxiang Chen Jun Dong Fengwang Ma Qingmei Guan State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple College of HorticultureNorthwest A&F UniversityYanglingShaanxi 712100China

Drought stress can negatively impact apple fruit quality and *** microRNAs(miRNAs)participate in apple tree and fruit development,as well as in biotic stress tolerance;however,it is largely unknown whether these molecules are involved in the drought *** identify drought-responsive miRNAs in Malus,we first examined the drought stress tolerance of ten F1 progenies of R3(M.×domestica)×*** performed Illumina sequencing on pooled total RNA from both drought-tolerant and drought-sensitive *** sequencing results identified a total of 206 known miRNAs and 253 candidate novel miRNAs from drought-tolerant plants and drought-sensitive plants under control or drought *** identified 67 miRNAs that were differentially expressed in drought-tolerant plants compared with drought-sensitive plants under drought *** drought stress,61 and 35 miRNAs were differentially expressed in drought-tolerant and drought-sensitive plants,*** determined the expression levels of seven out of eight miRNAs by stem-loop qPCR *** also predicted the target genes of all differentially expressed miRNAs and identified the expression of some *** Ontology analyses indicated that the target genes were mainly involved in stimulus response and cellular and metabolic ***,we confirmed roles of two miRNAs in apple response to *** results reveal candidate miRNAs and their associated mRNAs that could be targeted for improving drought tolerance in Malus species,thus providing a foundation for understanding the molecular networks involved in the response of apple trees to drought stress.

关键词： drought mannitol Malus

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基于自训练的半监督SQL注入检测算法

陕西师范大学学报（自然科学版） 2021年第1期49卷 37-43页

作者：解银朋周庆博何金栋谢新志周嵩计算机软件新技术国家重点实验室南京大学计算机科学与技术系江苏南京210023 国家电网福建省电力有限公司电力科学研究院福建福州350007

针对基于监督学习的SQL注入检测方法在某些场景下不适用的问题,本文提出一种基于自训练的半监督SQL注入检测方法(self-training based semi-supervised SQL injection detection,S4ID)。S4ID首先对SQL语句进行特征提取,包括基于语法树... 详细信息

针对基于监督学习的SQL注入检测方法在某些场景下不适用的问题,本文提出一种基于自训练的半监督SQL注入检测方法(self-training based semi-supervised SQL injection detection,S4ID)。S4ID首先对SQL语句进行特征提取,包括基于语法树的模式提取和基于词袋模型的特征向量表示;然后使用基于自训练的半监督算法进行训练,通过从未标记样本中选取部分样本并打上伪标记,实现训练集的扩充,从而改善机器学习模型。实验结果表明,在有标记样本有限的情况下,S4ID可以利用未标记样本,取得比监督学习方法更好的SQL注入检测效果。

关键词： SQL注入检测自训练机器学习半监督学习

苹果6-磷酸山梨醇脱氢酶基因启动子的转化及其转录活性

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西北农林科技大学学报（自然科学版） 2016年第9期44卷 166-173页

作者：杨国婵谢银鹏马锋旺梁东邹养军西北农林科技大学园艺学院陕西杨凌712100

【目的】研究6-磷酸山梨醇脱氢酶基因启动子(S6PDHp)的组织表达特点。【方法】利用Gateway技术构建了S6PDHp与GUS基因的融合表达载体,然后通过农杆菌介导法转化番茄"中蔬5号",对转基因植株进行PCR检测,对鉴定为阳性的植株进行GUS组织化... 详细信息

【目的】研究6-磷酸山梨醇脱氢酶基因启动子(S6PDHp)的组织表达特点。【方法】利用Gateway技术构建了S6PDHp与GUS基因的融合表达载体,然后通过农杆菌介导法转化番茄"中蔬5号",对转基因植株进行PCR检测,对鉴定为阳性的植株进行GUS组织化学染色,验证S6PDHp的组织表达特性。【结果】将S6PDHp-GUS融合表达载体转化番茄,经PCR检测显示,成功获得了转S6PDHp基因的阳性番茄植株。对阳性植株各组织器官的GUS化学染色和活性检测结果显示,除叶片外许多器官都有很强的GUS活性,且在转基因番茄成熟期植株的茎部活性最高,根部活性最低;叶片从新叶到老叶的各个时期,GUS活性持续降低。【结论】S6PDHp表达在植物衰老过程中降低;且在转基因番茄茎部的GUS活性最强,说明该启动子具有组织表达特异性。

关键词：苹果 6-磷酸山梨醇脱氢酶启动子转基因 GUS染色

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基于哈希学习的异常SQL检测

计算机应用 2021年第1期41卷 121-126页

作者：李明威蒋庆远解银朋何金栋吴丹计算机软件新技术国家重点实验室(南京大学) 南京210023 国家电网福建省电力有限公司电力科学研究院福州350007

针对最近邻(NN)方法在异常结构化查询语句(SQL)检测应用中面临的存储开销大、检索速度慢的问题,提出了一种基于哈希学习的异常SQL检测(HMSD)方法。该算法利用哈希学习来学习查询SQL语句的二值编码表示。首先,对查询SQL语句进行清洗去重... 详细信息

针对最近邻(NN)方法在异常结构化查询语句(SQL)检测应用中面临的存储开销大、检索速度慢的问题,提出了一种基于哈希学习的异常SQL检测(HMSD)方法。该算法利用哈希学习来学习查询SQL语句的二值编码表示。首先,对查询SQL语句进行清洗去重,从而将查询SQL语句表示为实值特征形式;然后利用等方差哈希方法来学习查询SQL语句的二值编码表示;最后,通过二值编码表示进行检索并提高异常SQL检测的速度。实验结果表明,在异常SQL检测数据集Wafamole上,将数据集进行随机划分,使训练集包含10000条SQL语句,测试集包含30000条SQL语句,在128比特长度下,与最近邻方法相比,所提算法的检测精度提高了1.3%,假正例率(FPR)降低了0.19%,假负例率(FNR)降低了2.41%,检索时间减少了94%,存储开销降低了97.5%;与支持向量机方法相比,所提算法的检测精度提高了0.17%,验证了所提算法能解决最近邻方法在异常SQL检测中存在的问题。

关键词：异常SQL检测最近邻二值编码表示哈希学习大规模检索

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铕荧光配合物的合成及其在手印显现中的应用

影像科学与光化学 2018年第6期36卷 498-506页

作者：张战胜高杨晨沈敦璞谢垠蓬李明王猛中国刑事警察学院痕迹检验技术系辽宁沈阳110035 上海市公安局普陀分局刑侦支队刑科所上海200333 痕迹检验鉴定技术公安部重点实验室辽宁沈阳110035

分别以邻、间、对甲基苯甲酸(o-、m-、p-MA)为第一配体、邻菲罗啉(o-Phen)为第二配体,利用沉淀法合成了一系列稀土铕的三元荧光配合物。对该系列稀土铕荧光配合物的合成条件(配体种类、配体用量、体系pH、反应温度、反应时间)进行了优... 详细信息

分别以邻、间、对甲基苯甲酸(o-、m-、p-MA)为第一配体、邻菲罗啉(o-Phen)为第二配体,利用沉淀法合成了一系列稀土铕的三元荧光配合物。对该系列稀土铕荧光配合物的合成条件(配体种类、配体用量、体系pH、反应温度、反应时间)进行了优化。将最优条件下合成的[Eu(m-MA)_3(o-Phen)]荧光配合物粉末应用到常见光滑客体表面潜在手印的显现技术中。从显现的对比度、灵敏度、选择性等方面对手印显现效果进行了详细考察。结果表明,显现后的手印在254nm紫外光的激发下,手印与客体之间的对比反差强烈,乳突纹线连贯清晰、细节特征反映明显,粉末只与乳突纹线部位吸附,基本不与小犁沟部位吸附。因此,本研究中基于铕荧光配合物的手印显现具有较强的对比度、灵敏度、选择性。

关键词：手印显现潜在手印铕配合物荧光材料

木薯渣对22～49日龄仔鹅生长性能、屠宰性能及内脏器官发育的影响

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动物营养学报 2015年第6期27卷 1804-1812页

作者：张芬芬王志跃杨海明李彦品马尹鹏谢燕娟扬州大学动物科学与技术学院扬州225009

本试验旨在研究饲粮中添加不同水平木薯渣对22～49日龄仔鹅生长性能、屠宰性能及内脏器官发育的影响。选取300只19日龄健康、体重相近的扬州鹅公鹅，随机分为5组，每组6个重复，每个重复10只。对照组饲喂基础饲粮，试验组分别饲喂添加5%... 详细信息

本试验旨在研究饲粮中添加不同水平木薯渣对22～49日龄仔鹅生长性能、屠宰性能及内脏器官发育的影响。选取300只19日龄健康、体重相近的扬州鹅公鹅，随机分为5组，每组6个重复，每个重复10只。对照组饲喂基础饲粮，试验组分别饲喂添加5%、10%、15%和20%木薯渣的试验饲粮，试验期28 d。结果表明：1）与对照组相比，饲粮中添加木薯渣显著提高22～28日龄、29～35日龄、22～49日龄仔鹅的平均日采食量和22～28日龄、22～49日龄仔鹅的平均日增重（ P〈0.05），其中22～49日龄仔鹅的平均日增重与木薯渣添加水平呈二次曲线关系（ P=0.017），达到最高平均日增重的木薯渣添加水平是12.4%。2）与对照组相比，饲粮中添加木薯渣显著降低仔鹅的肌胃指数和十二指肠的相对重量（ P〈0.05）。3）对各组饲粮增重成本降低的百分比进行曲线模拟，结果呈现二次曲线关系（ P〈0.05）；当饲粮中木薯渣添加水平为17.9%时，增重成本降低百分比值最大。由此可见，饲粮中添加木薯渣可以促进22～49日龄仔鹅生长，降低饲料成本，对肌胃、十二指肠的发育有一定的影响。根据饲粮中添加木薯渣对平均日增重最高限量和饲料增重成本的最低限量，建议添加水平为12.4%～17.9%。

关键词：木薯渣仔鹅生长性能屠宰性能内脏发育经济效益