Genome-wide identification of droughtresponsive microRNAs in two sets of Malus from interspecific hybrid progenies
作者机构：State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of AppleCollege of HorticultureNorthwest A&F UniversityYanglingShaanxi 712100China
出 版 物：《Horticulture Research》 (园艺研究（英文）)
年 卷 期：2019年第6卷第1期
页 面：794-811页
核心收录：
学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 0901[农学-作物学] 0703[理学-化学] 100214[医学-肿瘤学] 0836[工学-生物工程] 0902[农学-园艺学] 0834[工学-风景园林学（可授工学、农学学位）] 10[医学]
基 金：supported by the National Natural Science Foundation of China(31622049) the Project of Shaanxi Provincial Youth Science and Technology Star Plan(2015kjxx-14) the Key Program of the National Natural Science Foundation of China(31330068)
摘 要：Drought stress can negatively impact apple fruit quality and *** microRNAs(miRNAs)participate in apple tree and fruit development,as well as in biotic stress tolerance;however,it is largely unknown whether these molecules are involved in the drought *** identify drought-responsive miRNAs in Malus,we first examined the drought stress tolerance of ten F1 progenies of R3(M.×domestica)×*** performed Illumina sequencing on pooled total RNA from both drought-tolerant and drought-sensitive *** sequencing results identified a total of 206 known miRNAs and 253 candidate novel miRNAs from drought-tolerant plants and drought-sensitive plants under control or drought *** identified 67 miRNAs that were differentially expressed in drought-tolerant plants compared with drought-sensitive plants under drought *** drought stress,61 and 35 miRNAs were differentially expressed in drought-tolerant and drought-sensitive plants,*** determined the expression levels of seven out of eight miRNAs by stem-loop qPCR *** also predicted the target genes of all differentially expressed miRNAs and identified the expression of some *** Ontology analyses indicated that the target genes were mainly involved in stimulus response and cellular and metabolic ***,we confirmed roles of two miRNAs in apple response to *** results reveal candidate miRNAs and their associated mRNAs that could be targeted for improving drought tolerance in Malus species,thus providing a foundation for understanding the molecular networks involved in the response of apple trees to drought stress.
维普期刊数据库