检索结果-南通市图书馆

基于网络药理学探讨舒肝宁注射液治疗乙型病毒性肝炎的作用机制

在线全文

学校读者我要写书评

暂无评论

现代药物与临床 2024年第3期39卷 595-603页

作者：张立娜张向宇朱雨晴刘玉华马颖钰赵海亮河北医科大学第三医院河北石家庄050051 天津中医药大学天津301617 河北省医学情报研究所河北石家庄050071 河北省儿童医院河北石家庄050031

目的基于网络药理学和分子对接技术探究舒肝宁注射液治疗乙型病毒性肝炎的网络调控机制。方法选取舒肝宁注射液中19个代表性化学成分为研究对象,通过TCMSP、Swiss Target Prediction数据库,获得化合物潜在作用靶点。利用DisGeNET、GeneC... 详细信息

目的基于网络药理学和分子对接技术探究舒肝宁注射液治疗乙型病毒性肝炎的网络调控机制。方法选取舒肝宁注射液中19个代表性化学成分为研究对象,通过TCMSP、Swiss Target Prediction数据库,获得化合物潜在作用靶点。利用DisGeNET、GeneCards数据库检索乙型病毒性肝炎的疾病相关靶点,利用Venny平台获取化合物作用靶点与疾病靶点的交集靶点为舒肝宁注射液治疗乙型病毒性肝炎潜在作用靶点。进一步对靶蛋白进行蛋白相互作用(PPI)网络分析、Cluster模块分析、基因本体(GO)、京都基因与基因组百科全书(KEGG)分析。在PPI核心靶点网络分析基础上进一步通过分子对接方法验证网络药理结果。结果获得19个成分的173个舒肝宁注射液治疗乙型病毒性肝炎的潜在作用靶点,并可作用于SRC、HSP90AA1、ERS1、PIK3CA、HRAS等核心靶点,调节乙型病毒性肝炎、丙型病毒性肝炎、VEGF、TNF、PI3K/Akt等信号通路。分子对接结果表明,舒肝宁注射液与乙型病毒性肝炎信号通路关联的活性成分与相关靶点可自发结合。结论舒肝宁注射液治疗乙型病毒性肝炎的机制可能是通过多组分多靶点干预乙型肝炎信号通路起作用。

关键词：舒肝宁注射液乙型病毒性肝炎网络药理学作用机制腺苷异绿原酸C 栀子苷

Human hair follicle-derived mesenchymal stem cells promote tendon repair in a rabbit Achilles tendinopathy model

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2023年第9期136卷 1089-1097页

作者： yingyu ma Zhiwei Lin Xiaoyi Chen Xin Zhao Yi Sun Ji Wang Xiaozhou Mou Hai Zou Jinyang Chen Plastic and Reconstructive Surgery Center Department of Plastic and Reconstructive SurgeryZhejiang Provincial People’s HospitalAffiliated People’s Hospital of Hangzhou Medical CollegeHangzhouZhejiang 310014China Key Laboratory of Gastroenterology of Zhejiang Province Zhejiang Provincial People’s HospitalAffiliated People’s HospitalHangzhou Medical CollegeHangzhouZhejiang 310014China Clinical Research Institute Zhejiang Provincial People’s HospitalAffiliated People’s Hospital of Hangzhou Medical CollegeHangzhouZhejiang 310014China Zhejiang Healthfuture Biomedicine Co. LtdHangzhouZhejiang 310052China Department of Critical Care Fudan University Shanghai Cancer CenterShanghai 200032China Department of Oncology Shanghai Medical CollegeFudan UniversityShanghai 200032China

Background:Hair follicles are easily accessible and contain stem cells with different developmental origins,including mesenchymal stem cells(MSCs),that consequently reveal the potential of human hair follicle(hHF)-derived MSCs in repair and ***,the role of hHF-MSCs in Achilles tendinopathy(AT)remains *** present study investigated the effects of hHF-MSCs on Achilles tendon repair in ***:First,we extracted and characterized ***,a rabbit tendinopathy model was constructed to analyze the ability of hHF-MSCs to promote repair in *** observation and pathological and biomechanical analyses were performed to determine the effect of hHF-MSCs on AT,and quantitative real-time polymerase chain reaction,enzyme-linked immunosorbent assay,and immunohistochemical staining were performed to explore the molecular mechanisms through which hHF-MSCs affects ***,statistical analyses were performed using independent sample t test,one-way analysis of variance(ANOVA),and one-way repeated measures multivariate ANOVA as ***:Flow cytometry,a trilineage-induced differentiation test,confirmed that hHF-derived stem cells were derived from *** effect of hHF-MSCs on AT revealed that the Achilles tendon was anatomically healthy,as well as the maximum load carried by the Achilles tendon and hydroxyproline proteomic levels were ***,collagen I and III were upregulated in rabbit AT treated with hHF-MSCs(compared with AT group;Pmatrix metalloproteinase(MMP)-9 ***:hHF-MSCs can be a treatment modality to promote AT repair in rabbits by upregulating collagen I and *** analysis revealed that treatment of AT using hHF-MSCs promoted the regeneration of collagen fiber,possibly because of upregulation of TNC and downregulation

关键词： Human hair follicle-derived mesenchymal stem cells Tendinopathy Collagen type I Collagen type III

蒸馏水清除原发性肝癌切除术中腹腔脱落癌细胞的量效关系

在线全文

学校读者我要写书评

暂无评论

中华医学杂志 2016年第46期96卷 3702-3705页

作者：徐继喻晓芬马英玉邵钦树浙江省人民医院胃肠胰外科杭州310014 浙江省人民医院实验室

目的探讨温热蒸馏水清除116例原发性肝癌切除术中腹腔内脱落癌细胞的量效关系。方法对2012年1月—2015年12月手术治疗的116例原发性肝癌手术患者于肿瘤切除前后检测腹腔脱落癌细胞阳性率；关腹前用43～45℃蒸馏水1500ml／次冲洗腹腔后，... 详细信息

目的探讨温热蒸馏水清除116例原发性肝癌切除术中腹腔内脱落癌细胞的量效关系。方法对2012年1月—2015年12月手术治疗的116例原发性肝癌手术患者于肿瘤切除前后检测腹腔脱落癌细胞阳性率；关腹前用43～45℃蒸馏水1500ml／次冲洗腹腔后，用37℃无菌生理盐水200ml行腹腔灌洗并收集灌洗液，分别检测腹腔脱落癌细胞阳性率。结果≤10cm肿瘤切除前后腹腔脱落癌细胞无显著变化（均P〉0．05）；〉10cm肿瘤相比切除前，切除后腹腔脱落细胞显著增加（〉10-≤15cm组，P=0．044；〉15（3m组，P=0．033），随着肿瘤体积的增大，肿瘤切除后肿瘤脱落细胞显著增加（P〈0．001），冲洗后残留率增加，〉10cm的肿瘤随着冲洗次数增加，残留率显著下降（〉10～≤15cm组，P=0．002；〉15cm组，P=0．001），4次冲洗后检测不到残留。≤5cm肿瘤随着冲洗次数增加残留率下降不明显（P〉0．05），但均在第3次冲洗后未检测到残留。结论彻底清除原发性肝癌术中腹腔脱落癌细胞的冲洗量与肿瘤体积明显相关。

关键词：原发性肝细胞癌腹腔脱落癌细胞量效关系

Discovery of active compounds and key targets of Thymus quinquecostatus Celak. based on gastrointestinal metabolism and Gut flora-Compound-Target-Pathway network with TOPSIS method

在线全文

学校读者我要写书评

暂无评论

Food Science and Human Wellness 2024年

作者： Xueyang Ren Jiamu ma Ying Dong Yuan Zheng Rufeng Wang Chongjun Zhao Wei Liu Mingxia Li Mengyu Sun Feng Zhang yingyu He Xianxian Li Qingyue Deng Gaimei She School of Chinese materia Medica， Beijing University of Chinese Medicine School of Life Sciences， Beijing University of Chinese Medicine Beijing Key Laboratory for Quality Evaluation of Chinese materia Medica

Thymus quinquecostatus Celak.， a traditional aromatic edible plant from Lamiaceae， is widely used as food additive， food condiment， spice， and herbal teas. Its polyphenol-rich fraction （PRF） has been proven to be effective protective effect for cerebral ischemia reperfusion injury （CIRI） in our previous study. In this study， we developed a novel “Gut flora-Compound-Target-Pathway” （GCTP） network based on network pharmacology coupled with gastrointestinal metabolism for screening bio-active components， key targets and gut floras through the classical technique for order preference by similarity to ideal solution （TOPSIS） method. This compensates for the lack of gut floras and gastrointestinal metabolism in network pharmacology. Firstly， four incubation models covering simulated gastric juice， simulated intestinal juice， gut floras of normal and transient middle cerebral artery occlusion （tMCAO） rat in vitro were applied to PRF. The 109 proto-components and 64 metabolites were elucidated by UPLC-QE-Orbitrap-MSn. Then， the key targets of MMP9， PTGS2， FYN， ESR1， APP， and EGFR， and gut floras of Enterococcus avium LY1 were selected. Moreover， the selected key proto-components were rosmarinic acid， daidzein， quercetin， luteolin， apigenin， methyl rosmarinate， kaempferol， luteoloside， and caffeic acid， and the key metabolites were isokaempferide， isorhamnetin， isoquercetin， and mangiferin. Binding of compounds to the key proteins was analyzed by molecular docking， and also verified though a AAPH induced oxidative stress zebrafish model and qRT-PCR assays. This study provides a new idea and a better understanding of PRF for its protective effects on CIRI and its underlying mechanisms.

关键词：

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

太赫兹与水的相互作用:机理、应用和新趋势

深圳大学学报（理工版） 2019年第2期36卷 200-206页

作者：范姝婷马莹玉舒国响钱正芳深圳大学物理与光电工程学院广东深圳518060 深圳大学电子与信息工程学院广东深圳518060

自21世纪以来,太赫兹技术因受到世界各国的重视和扶持而取得迅猛发展.介绍液态水分子网络在太赫兹波段的振动和弛豫模式,以及太赫兹波与水分子之间相互作用的原理.评述基于此机理太赫兹在生物医学,尤其是关于癌症检测领域的发展与挑战,... 详细信息

自21世纪以来,太赫兹技术因受到世界各国的重视和扶持而取得迅猛发展.介绍液态水分子网络在太赫兹波段的振动和弛豫模式,以及太赫兹波与水分子之间相互作用的原理.评述基于此机理太赫兹在生物医学,尤其是关于癌症检测领域的发展与挑战,介绍了本课题组目前开展的工作.展望围绕太赫兹波与水相互作用产生的新研究与新技术.

关键词：电磁波物理太赫兹太赫兹时域光谱太赫兹成像生物医学应用癌症检测水分子网络

在线全文

学校读者我要写书评

暂无评论

猎头人才服务网站的设计与开发

计算机应用文摘 2024年第7期40卷 43-45页

作者：李江蕾杨威吴国光马英峪徐存伯丁酩绅沈阳城市建设学院信息与控制工程学院沈阳110167

我国猎头行业已进入资源抢夺期,其中对顶尖稀缺人才和高级复合型人才的争夺越来越激烈。在经济全球化背景下,企业对头部人才的需求明显大于对中等人才需求,人力资源的“橄榄”效果愈发明显。因此,文章开发了连接高级人才、企业人力资源... 详细信息

我国猎头行业已进入资源抢夺期,其中对顶尖稀缺人才和高级复合型人才的争夺越来越激烈。在经济全球化背景下,企业对头部人才的需求明显大于对中等人才需求,人力资源的“橄榄”效果愈发明显。因此,文章开发了连接高级人才、企业人力资源的猎头人才服务网站,可在一定程度上满足双方的迫切需求。

关键词：猎头服务人才招聘网络招聘

Fufang E’jiao Jiang’s effect on immunity,hematopoiesis,and angiogenesis via a systematic“compound-effect-target”analysis

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Food Science and Human Wellness 2024年第5期13卷 2813-2832页

作者： Xiang Li Xiao Xu Ying Dong Shusheng Fan Xueyang Ren Yuan Zheng Jiamu ma Feng Zhang Qingyue Deng Xianxian Li yingyu He Mengyu Sun Wei Liu Mengxia Li Qing Xia Yan Zhang Gaimei She School of Chinese materia Medica Beijing University of Chinese MedicineBeijing 102488China Biology Institute Qilu University of Technology(Shandong Academy of Sciences)Jinan 250103China National Engineering Research Center for Gelatin-based Traditional Chinese Medicine Dong-E-E-Jiao Co.Ltd.Liaocheng 252201China

Fufang E’jiao Jiang(FEJ)as a healthy food consisting of medicine food homology materials approved by China’s Ministry of Health has been extensively applied to replenish qi and nourish blood,and it has a positive impact on women’s *** find out the material basis and mechanism of FEJ,a systematic“compoundeffect-target”analysis including chemical composition resolution,zebrafish,network pharmacology,molecular docking,transcriptome,and bibliometric analysis was adopted.124 chemical components including ginsenosides,and phenylethanoid glycosides in FEJ were discovered,and effects of FEJ on promoting the generation of immune cells,erythropoiesis and angiogenesis in zebrafish were *** on network pharmacology,molecular docking and in vivo activity assay,6 compounds including jionoside A1,isoacteoside,echinacoside,acteoside,lobetyolin,and rehmannioside D were identified as active components of *** data showed that several pathways such as complement and coagulation cascades,ECM-receptor interaction,and PI3K-Akt signaling pathway were associated with proangiogenic effect of FEJ.19 common targets were obtained through combined analysis of network pharmacology and transcriptomics,and 5 targets of them were verified by *** bibliometric analysis of these common targets revealed that FEJ was related to energy metabolism,pathway in cancer,etc.,which was consistent with the results of network pharmacology and *** studies suggested that FEJ could replenish qi and nourish blood through multi-compound and multi-targets.

关键词： Fufang E’jiao Jiang Zebrafish(Danio rerio) Network pharmacology Transcriptome Bibliometrics

同方期刊数据库

原发性肝细胞癌92例患者腹腔脱落癌细胞阳性率分析

在线全文

学校读者我要写书评

暂无评论

中华肝胆外科杂志 2014年第1期20卷 20-23页

作者：喻晓芬叶再元马英玉张琴芳胡弦琴浙江省人民医院手术室杭州310014 浙江省人民医院胃肠外科杭州310014 浙江省人民医院实验室杭州310014

目的探讨原发性肝细胞癌侵入性操作前腹腔脱落癌细胞阳性率与临床病理特征的关系.方法92例原发性肝细胞癌患者开腹后有腹腔积液者直接收集腹腔积液,无腹腔积液者收集腹腔灌洗液,检测腹腔脱落癌细胞的阳性率.结果腹腔脱落癌细胞阳性检出... 详细信息

目的探讨原发性肝细胞癌侵入性操作前腹腔脱落癌细胞阳性率与临床病理特征的关系.方法92例原发性肝细胞癌患者开腹后有腹腔积液者直接收集腹腔积液,无腹腔积液者收集腹腔灌洗液,检测腹腔脱落癌细胞的阳性率.结果腹腔脱落癌细胞阳性检出率与TNM分期、肿瘤位置、肿瘤大小显著相关.TNM分期Ⅲ、Ⅳ期肝癌腹腔脱落癌细胞阳性检出率显著高于Ⅰ、Ⅱ期（38.1％比8.0％,P=0.0005）.肿瘤位置越接近表面（P=0.0002）、直径越大（P=0.00007）,腹腔脱落癌细胞阳性检出率越高.结论腹腔脱落癌细胞与肝细胞癌分期进展、肿瘤位置、大小明显相关.

关键词：原发性肝细胞癌腹腔脱落癌细胞腹腔积液腹腔灌洗液

在线全文

学校读者我要写书评

暂无评论

蛋氨酸对化疗药物引起内耳损伤的保护作用

河北医科大学学报 2012年第8期33卷 904-907页

作者：黄瑛马颖钰张璞林曦曲雁云南省昆明市第一人民医院耳鼻咽喉科云南昆明650000 河北医科大学第三医院耳鼻咽喉科河北石家庄050051 美国Emory大学医学院耳鼻咽喉头颈外科乔治亚州亚特兰大303223030

目的研究蛋氨酸对化疗药物顺铂导致耳毒性的保护作用。方法将30只昆明小鼠随机分为3组。顺铂组(n=10):给予顺铂3.5mg·kg^(-1)·d^(-1)腹腔注射,连续7d;蛋氨酸+顺铂组(n=10):蛋氨酸300mg·kg^(-1)·d^(-1)腹腔注射,30min后给予顺铂3.5mg... 详细信息

目的研究蛋氨酸对化疗药物顺铂导致耳毒性的保护作用。方法将30只昆明小鼠随机分为3组。顺铂组(n=10):给予顺铂3.5mg·kg^(-1)·d^(-1)腹腔注射,连续7d;蛋氨酸+顺铂组(n=10):蛋氨酸300mg·kg^(-1)·d^(-1)腹腔注射,30min后给予顺铂3.5mg·kg^(-1)·d^(-1)腹腔注射,连续7d;对照组(n=10):生理盐水3.5mg·kg^(-1)·d^(-1)腹腔注射,连续7d。实验第8天,利用听觉脑干诱发电位(auditory brainstem response,ABR)对3组小鼠进行听功能测试,然后迅速断头取出双侧内耳,解剖耳蜗基底膜,铺片并HE染色,普通光学显微镜下观察。结果在ABR测试中,对照组小鼠实验前后听力无明显变化;顺铂组小鼠听阈及Ⅰ波和Ⅲ波潜伏期与对照组相比变化明显;蛋氨酸+顺铂组小鼠听阈及Ⅰ波和Ⅲ波潜伏期与对照组相比也出现变化,但变化程度较顺铂组明显减轻。光镜观察:对照组动物耳蜗基底膜外毛细胞排列整齐;顺铂组基底膜外毛细胞有不同程度的坏死、脱落,尤以底转缺失严重;蛋氨酸+顺铂组耳蜗毛细胞损伤较顺铂组明显减轻。结论化疗药物顺铂对耳蜗毛细胞引起损害,而蛋氨酸对这种损害有较好的保护作用。

关键词：内耳 S-腺苷甲硫氨酸顺铂