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Ectopic Overexpression of Wheat Adenosine Diphosphate-ribosylation Factor,TaARF,Increases Growth Rate in Arabidopsis

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Journal of Integrative Plant Biology 2009年第1期51卷 35-44页

作者： yingyin yao Zhongfu Ni Jinkun Du Zongfu Han Yan Chen Qingbo Zhang Qixin Sun Department of Plant Genetics & Breeding and State Key Laboratory for Agrobiotechnology Key Laboratory of Crop Heterosis and UtilizationMinistry of Education Key Laboratory of Crop Genomics and Genetic Improvement Ministry of Agdculture Beijing Key Laboratory of Crop Genetic Improvement China Agricultural University Beijing 100094 China

Differential gene expression between hybrids and their parents is considered to be associated with heterosis. However, the physiological functions and possible contribution to heterosis of these differentially expressed genes are unknown. We have isolated one hybrid upregulated gene encoding putative wheat ADP-ribosylation factor, designated TaARF. In this study, real-time quantitative reverse transcription-polymerase chain reaction analysis indicated that the TaARF transcript was preferentially expressed in root, node and crown, and the accumulation of TaARF mRNA in hybrid was more than 1.5-fold higher than that in two parents. In order to understand possible roles of the putative wheat ARF gene, TaARF was overexpressed in Arabidopsis, and the transgenic plants were characterized. We show that ectopic overexpression of TaARF in Arabidopsis leads to increased leaf area, increased growth rate and earlier transition to flowering, suggesting that TaARF plays significant roles in growth and development. This study provides evidence demonstrating that TaARF plays important roles in growth and development and we speculate that the upregulated expression of this gene might contribute to the heterosis observed in wheat root and leaf growth.

关键词： adenosine diphosphate-ribosylation factor Arabidopsis ectopic heterosis overexpression wheat.

The genetic and molecular basis for improving heat stress tolerance in wheat

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aBIOTECH 2022年第1期3卷 25-39页

作者： Lv Sun Jingjing Wen Huiru Peng yingyin yao Zhaorong Hu Zhongfu Ni Qixin Sun Mingming Xin Key Laboratory of Crop Heterosis Utilization(MOE) China Agricultural UniversityBeijing 100193China

Wheat production requires at least-2.4%increase per year rate by 2050 globally to meet food ***,heat stress results in serious yield loss of wheat ***,wheat has evolved genetic basis and molecular mechanisms to protect themselves from heat-induced ***,it is very urgent to understand the underlying genetic basis and molecular mechanisms responsive to elevated temperatures to provide important strategies for heat-tolerant varieties *** this review,we focused on the impact of heat stress on morphology variation at adult stage in wheat breeding *** also summarize the recent studies of genetic and molecular factors regulating heat tolerance,including identification of heat stress tolerance related QTLs/genes,and the regulation pathway in response to heat *** addition,we discuss the potential ways to improve heat tolerance by developing new technologies such as genome *** review of wheat responses to heat stress may shed light on the understanding heat-responsive mechanisms,although the regu-latory network of heat tolerance is still ambiguous in wheat.

关键词： Wheat Heat stress Genetic basis Molecular mechanisms

Transcriptome Comparison of Susceptible and Resistant Wheat in Response to Powdery Mildew Infection

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Genomics, Proteomics & Bioinformatics 2012年第2期10卷 94-106页

作者： Mingming Xin Xiangfeng Wang Huiru Peng yingyin yao Chaojie Xie yao Han ZhongfuNi Qixin Sun State Key Laboratory for Agrobiotechnology and MOE Key Laboratory of Crop Heterosis and Utilization and MOA Key Laboratory of Crop Genomics and Genetic Improvement Beijing Key Laboratory of Crop Genetic ImprovementChina Agricultural UniversityBeijing 100193China National Plant Gene Research Centre(Beijing) Beijing 100094China School of Plant Sciences University of ArizonaTucsonAZ 85721USA

Powdery mildew （Pro） caused by the infection of Blumeria graminis f. sp. tritici （Bgt） is a worldwide crop disease resulting in significant loss of wheat yield. To profile the genes and pathways responding to the Bgt infection, here, using Affymetrix wheat microarrays, we compared the leaf transcriptomes before and after Bgt inoculation in two wheat genotypes, a Pm-susceptible cultivar Jingdong 8 （S） and its near-isogenic line （R） carrying a single Pm resistant gene Pm30. Our analysis showed that the original gene expression status in the S and R genotypes of wheat was almost identical before Bgt inoculation, since only 60 genes exhibited differential expression by P = 0.01 cutoff. However, 12 h after Bgt inoculation, 3014 and 2800 genes in the S and R genotype, respectively, responded to infec- tion. A wide range of pathways were involved, including cell wall fortification, flavonoid biosynthesis and metabolic processes. Further- more, for the first time, we show that sense-antisense pair genes might be participants in wheat-powdery mildew interaction. In addition, the results of qRT-PCR analysis on several candidate genes were consistent with the microarray data in their expression patterns. In summary, this study reveals leaf transcriptome changes before and after powdery mildew infection in wheat near-isogenic lines, suggest- ing that powdery mildew resistance is a highly complex systematic response involving a large amount of gene regulation.

关键词： Wheat Pathogen resistance Powdery mildew Microarrays

Genome-wide linkage mapping of QTL for root hair length in a Chinese common wheat population

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The Crop Journal 2020年第6期8卷 1049-1056页

作者： Feng Huang Zhaoyan Chen Dejie Du Panfeng Guan Lingling Chai Weilong Guo Zhaorong Hu Mingming Xin Huiru Peng yingyin yao Zhongfu Ni Key Laboratory of Crop Heterosis and Utilization(MOE)/State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Genetic Improvement China Agricultural UniversityBeijing 100193China National Plant Gene Research Center Beijing 100193China Zhoukou Academy of Agriculture Sciences Zhoukou 466001HenanChina

Root hairs are fast growing,ephemeral tubular extensions of the root epidermis that aid nutrient and water *** aim of the present study was to identify QTL for root hair length(RHL)using 227 F8 recombinant inbred lines(RILs)derived from a cross of Zhou 8425 B(Z8425 B)and Chinese Spring(CS),and to develop convenient molecular markers for markerassisted breeding in *** of variance of root hair length showed significant differences(P2.5)for RHL were identified on chromosomes 1 B(2),2 D and 6 D and four putative QTL(2≤LOD≤2.5)were detected on chromosomes 1 A,3 A,6 B,and 7 B,explaining 3.32%–6.52%of the phenotypic *** positive alleles for increased RHL of QTL on chromosomes 2 D,6 B and 6 D(***-2 D,q ***-6 B,and ***-6 D)were contributed by Z8425 B,and CS contributed positive QTL alleles on chromosomes 1 A(q ***-1 A),1 B(***-1 B.1 and ***-1 B.2),3 A(q ***-3 A)and 7 B(q ***-7 B).STARP markers were developed for ***-1 B.1,***-2 D,***-6 D,and q ***-7 *** and association analysis indicated that the positive allele of ***-6 D had been strongly selected in Chinese wheat breeding ***,the identified QTL for root hair length are likely to be useful for marker-assisted selection.

关键词： GWAS QTL mapping STARP marker Triticum aestivum

Characterization of a new hexaploid triticale 6D(6A) substitution line with increased grain weight and decreased spikelet number

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The Crop Journal 2019年第5期7卷 598-607页

作者： Zhiyu Feng Zhongqi Qi Dejie Du Mingyi Zhang Aiju Zhao Zhaorong Hu Mingming Xin yingyin yao Huiru Peng Qixin Sun Zhongfu Ni Key Laboratory of Crop Heterosis and Utilization State Key Laboratory for AgrobiotechnologyBeijing Key Laboratory of Crop Genetic ImprovementCollege of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China Institute of Wheat Research Shanxi Academy of Agricultural SciencesLinfen 041000ShanxiChina Hebei Crop Genetic Breeding Laboratory Institute of Cereal and Oil CropsHebei Academy of Agriculture and Forestry SciencesShijiazhuang 050035HebeiChina

Hexaploid triticale(×Triticosecale,AABBRR)is an important forage crop and a promising energy *** D-genome chromosomes or segments from common wheat(Triticum aestivum)into hexaploid triticale is attractive in improving its economically important ***,a hexaploid triticale 6D(6A)substitution line Lin 456 derived from the cross between the octoploid triticale line H400 and the hexaploid wheat Lin 56 was identified and analyzed by genomic in situ hybridization(GISH),fluorescence in situ hybridization(FISH),and molecular *** GISH analysis showed that Lin 456 is a hexaploid triticalewith 14 rye(Secale cereale)chromosomes and 28 wheat chromosomes,whereas non-denaturing fluorescence in situ hybridization(ND-FISH)and molecular marker analysis revealed that it is a 6D(6A)substitution *** contrast to previous studies,the signal of Oligo-pSc119.2 was observed at the distal end of 6DL in Lin *** wheat chromosome 6D was associatedwith increased grain weight and decreased spikelet number using the genotypic data combined with the phenotypes of the F2 population in the three *** thousand-grain weight and grain width in the substitution individuals were significantly higher than those in the non-substitution individuals in the F2 population across the three *** propose that the hexaploid triticale 6D(6A)substitution line Lin 456 can be a valuable and promising donor stock for genetic improvement during triticale breeding.

关键词： In situ hybridization Spikelet number Substitution line Thousand-grain weight Triticale

Genomic insights into the origin and evolution of spelt(Triticum spelta L.)as a valuable gene pool for modern wheat breeding

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Plant Communications 2024年第5期5卷 75-90页

作者： Yongfa Wang Zihao Wang Yongming Chen Tianyu Lan Xiaobo Wang Gang Liu Mingming Xin Zhaorong Hu yingyin yao Zhongfu Ni Qixin Sun Weilong Guo Huiru Peng Frontiers Science Center for Molecular Design Breeding Key Laboratory of Crop Heterosis and UtilizationBeijing Key Laboratory of Crop Genetic ImprovementChina Agricultural UniversityBeijing 100193China Institute for Plant Genetics Heinrich Heine University40225 DusseldorfGermany Sanya Institute of China Agricultural University Sanya 572025China

Spelt(Triticum aestivum ***)is an important wheat subspecies mainly cultivated in Europe before the 20th century that has contributed to modern wheat breeding as a valuable genetic ***,relatively little is known about the origins and maintenance of spelt ***,using resequencing data from 416 worldwide wheat accessions,including representative spelt wheat,we demonstrate that Eu-ropean spelt emerged when primitive hexaploid wheat spread to the west and hybridized with pre-settled domesticated emmer,the putative maternal *** introgression regions from domesticated emmer confer spelt’s primitive morphological characters used for species taxonomy,such as tenacious glumes and laterﬂ*** propose a haplotype-based"spelt index"to identify spelt-type wheat vari-eties and to quantify utilization of the spelt gene pool in modern wheat *** study reveals the ge-netic basis for the establishment of the spelt wheat subspecies in a specific ecological niche and the vital role of the spelt gene pool as a unique germplasm resource in modern wheat breeding.

关键词： wheat spelt interspecific hybridization gene pool modern wheat breeding

Cloning of a down-regulated gene encoding small GTP binding protein in hybrid wheat

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Progress in Natural Science:Materials International 2005年第7期15卷 621-626页

作者： yao yingyin, NI Zhongfu, CHEN Rongmin, WU Limin and SUN Qixin (Department of Plant Genetic & Breeding, State Key Laboratory for Agro-biotechnology, China Agricultural University, Beijing 100094, China) Department of Plant Genetic & Breeding State Key Laboratory for Agro-biotechnology China Agricultural University Beijing 100094 China

Previous studies showed that differential gene expression between wheat hybrids and their parents was responsible for the heterosis. To provide an insight into the molecular basis of wheat heterosis, one cDNA, designated TaRab, was identified from the cDNA library of wheat seedling leaves. The sequence comparison in GenBank revealed that TaRab is homologous to a group of genes encoding Rab-GTP binding protein. Semi-quantitative RT-PCR analysis indicated that TaRab was expressed in all plant tissues examined, but at slightly higher level in leaves. Further analysis exhibited that TaRab displayed lower expression in hybrid than in its patents in both roots and leaves, which was in agreement with the original results of suppression subtractive hybridization. TaRab was located on chromosome 7B and C-7DS5-0.36 by in silico mapping. The relationship between differential expression of TaRab and the molecular basis of wheat heterosis was also discussed.

关键词： wheat Rab protein gene expression heterosis.

A highly conserved amino acid in high molecular weight glutenin subunit 1Dy12 contributes to gluten functionality and processing quality in wheat

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Journal of Genetics and Genomics 2023年第11期50卷 909-912页

作者： Changfeng Yang Qian Chen Mingming Xin Zhenqi Su Jinkun Du Weilong Guo Zhaorong Hu Jie Liu Huiru Peng Zhongfu Ni Qixin Sun yingyin yao Frontiers Science Center for Molecular Design Breeding Key Laboratory of Crop Heterosis and Utilization(MOE)Beijing Key Laboratory of Crop Genetic ImprovementChina Agricultural UniversityBeijing 100193China

Bread wheat(Triticum aestivum L.)is one of the most commonly consumed staples worldwide,with widespread uses in foods such as breads,noodles,cakes,and cookies(Veraverbeke and Delcour,2002).The specific cooking properties of wheat products are mainly conferred by the gluten proteins contained in wheat grains(Delcour et al.,2012).High molecular weight glutenin subunits(HMW-GSs)are important constituents of wheat gluten proteins,largely determining gluten elasticity and processing quality(Branlard and Dardevet,1985;Payne et al.,1988).

关键词： weight wheat gluten

A wheat integrative regulatory network from large-scale complementary functional datasets enables trait-associated gene discovery for crop improvement

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Molecular Plant 2023年第2期16卷 393-414页

作者： Yongming Chen Yiwen Guo Panfeng Guan Yongfa Wang Xiaobo Wang Zihao Wang Zhen Qin Shengwei Ma Mingming Xin Zhaorong Hu yingyin yao Zhongfu Ni Qixin Sun Weilong Guo Huiru Peng Frontiers Science Center for Molecular Design Breeding Key Laboratory of Crop Heterosis and UtilizationBeijing Key Laboratory of Crop Genetic ImprovementChina Agricultural UniversityBeijing 100193China Hainan Yazhou Bay Seed Laboratory SanyaHainanChina State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijingChina

Gene regulation is central to all aspects of organism growth,and understanding it using large-scale functional datasets can provide a whole view of biological processes controlling complex phenotypic traits in ***,the connection between massive functional datasets and trait-associated gene discovery for crop improvement is still *** this study,we constructed a wheat integrative gene regulatory network(wGRN)by combining an updated genome annotation and diverse complementary functional datasets,including gene expression,sequence motif,transcription factor(TF)binding,chromatin accessibility,and evolutionarily conserved *** contains 7.2 million genome-wide interactions covering 5947 TFs and 127439 target genes,which were further verified using known regulatory relationships,condition-specific expression,gene functional information,and *** used wGRN to assign genome-wide genes to 3891 specific biological pathways and accurately prioritize candidate genes associated with complex phenotypic traits in genome-wide association *** addition,wGRN was used to enhance the interpretation of a spike temporal transcriptome dataset to construct high-resolution *** further unveiled novel regulators that enhance the power of spike phenotypic trait prediction using machine learning and contribute to the spike phenotypic differences among modern wheat ***,we developed an interactive webserver,wGRN(http://***/wGRN),for the community to explore gene regulation and discover trait-associated ***,this community resource establishes the foundation for using large-scale functional datasets to guide trait-associated gene discovery for crop improvement.

关键词： wheat integrative gene regulatory network functional gene discovery phenotype prediction crop improvement