Cloning of a down-regulated gene encoding small GTP binding protein in hybrid wheat

作     者：YAO Yingyin, NI Zhongfu, CHEN Rongmin, WU Limin and SUN Qixin (Department of Plant Genetic & Breeding, State Key Laboratory for Agro-biotechnology, China Agricultural University, Beijing 100094, China) 

作者机构：State Key Laboratory for Agro-biotechnology Department of Plant Genetic and Breeding China Agricultural University Beijing 100094 China 

出 版 物：《Progress in Natural Science:Materials International》 (自然科学进展·国际材料(英文))

年 卷 期：2005年第15卷第7期

页      面：621-626页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：Supported by the State Key Basic Research and Development Program of China (Grant No. 2001CB1088) National Fund for Distinguished Young Scholars (39925026) National Natural Science Foundation of China (Grant No. 30270824) 

主　　题：wheat Rab protein gene expression heterosis. 

摘      要：Previous studies showed that differential gene expression between wheat hybrids and their parents was responsible for the heterosis. To provide an insight into the molecular basis of wheat heterosis, one cDNA, designated TaRab, was identified from the cDNA library of wheat seedling leaves. The sequence comparison in GenBank revealed that TaRab is homologous to a group of genes encoding Rab-GTP binding protein. Semi-quantitative RT-PCR analysis indicated that TaRab was expressed in all plant tissues examined, but at slightly higher level in leaves. Further analysis exhibited that TaRab displayed lower expression in hybrid than in its patents in both roots and leaves, which was in agreement with the original results of suppression subtractive hybridization. TaRab was located on chromosome 7B and C-7DS5-0.36 by in silico mapping. The relationship between differential expression of TaRab and the molecular basis of wheat heterosis was also discussed.