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microRNA-mediated R gene regulation: molecular scabbards for double-edged swords

Science China(Life Sciences) 2018年第2期61卷 138-147页

作者： yingtian deng Minglei Liu Xiaofei Li Feng Li Key Laboratory of Horticultural Plant Biology (MOE) College of Horticulture and Forestry Sciences Huazhong Agricultural University

Plant resistance(R) proteins are immune receptors that recognize pathogen effectors and trigger rapid defense responses, namely effector-triggered immunity. R protein-mediated pathogen resistance is usually race specific. During plant-pathogen coevolution,plant genomes accumulated large numbers of R genes. Even though plant R genes provide important natural resources for breeding disease-resistant crops, their presence in the plant genome comes at a cost. Misregulation of R genes leads to developmental defects, such as stunted growth and reduced fertility. In the past decade, many microRNAs(miRNAs) have been identified to target various R genes in plant genomes. miRNAs reduce R gene levels under normal conditions and allow induction of R gene expression under various stresses. For these reasons, we consider R genes to be double-edged "swords" and miRNAs as molecular "scabbards". In the present review, we summarize the contributions and potential problems of these "swords" and discuss the features and production of the "scabbards", as well as the mechanisms used to pull the "sword" from the "scabbard"when needed.

关键词： NLR innate immunity siRNA miRNA crop disease breeding

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Mutation in the Agrobacterium his I gene enhances transient expression in pepper

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Horticultural Plant Journal 2024年第3期10卷 809-822页

作者： Dan Liu Shengnan Zhao Jubin Wang Xi Zhang yingtian deng Feng Li National Key Laboratory for Germplasm Innovation&Utilization of Horticultural Crops College of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhanHubei 430070China

Agrobacterium-mediated plant transformation is widely used in plant genetic ***,its efficiency is limited by plant immunity against *** pepper(Capsicum annuum L.)is an important vegetable that is recalcitrant to Agrobacterium-mediated *** this work,Agrobacterium was found to induce a strong immune response in pepper,which might be the reason for T-DNA being difficult to express in *** Agrobacterium mutant screen was conducted and a point mutation in the hisI gene was identified due to a weak immune response and enhanced transient expression mediated by this Agrobacterium mutant in pepper *** genetic analysis revealed that histidine biosynthesis deficiency caused by mutations in many genes of this pathway led to reduced pepper cell death,presumably due to reduced bacterial ***,mutation analysis of threonine and tryptophan biosynthesis genes showed that the biosynthesis of different amino acids may play different roles in Agrobacterium growth and stimulating the pepper immune *** possible application of Agrobacterium amino acid biosynthesis mutations in plant biology was discussed.

关键词： Capsicum annuum L. Agrobacterium Transient transformation efficiency Mutation Amino acid Immune response

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Pepper(Capsicum annuum)xylogen-like arabinogalactan protein(XYLP)1 and XYLP2 promote synthesis of lignin during stem development to cope with stresses

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Vegetable Research 2022年第1期2卷 138-147页

作者： Min Zhang Qianwen Zhang Long Cheng Qianying Li Xinyue He Kaixuan Wang Jianan Liu Feng Li yingtian deng Key Laboratory of Horticultural Plant Biology(Ministry of Education) College of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhan 430070China

Pepper stems exhibit a high level of strength and lignin deposition to support plant growth,which direct the cultivation style without binding sticks or ***,regulation of lignin synthesis and accumulation in pepper stem has not been extensively ***,we first investigated the pepper stem developmental process and confirmed that increasing lignin accumulation occurs during stem *** then performed genome-wide identification and characterization of xylogen-like arabinogalactan protein(XYLP)family members and obtained 10,22,and 19 XYLPs in pepper,tomato,and potato *** of the phylogenetic relationship among the identified XYLPs suggested that these proteins are conserved in ***,we analyzed the 10 CanXYLP genes and observed that these genes exhibit differential expressing patterns at different stages of pepper stem *** these genes,two XYLPs,namely CanXYLP1 and CanXYLP2,exhibited an increased expression pattern and a strong correlation with lignin accumulation in pepper *** further found that CanXYLP1 and CanXYLP2 play a role in pepper stem lignification by positively regulating the lignin synthesis pathway genes in pepper,and the CanXYLP1/2-silenced plants displayed a blocked lignification ***,we confirmed that CanXYLP1/2 expression is upregulated in response to some abiotic and biotic signals,suggesting that these two genes enhance the tolerance of pepper stem to unfavorable *** results contribute to our understanding of the molecular mechanism controlling pepper stem lignification,and the relationship between the lignin content of pepper stem and XYLPs.

关键词： fir pepper synthesis

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High-quality assembled and annotated genomes of Nicotiana tabacum and Nicotiana benthamiana reveal chromosome evolution and changes in defense arsenals

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Molecular Plant 2024年第3期17卷 423-437页

作者： Jubin Wang Qingling Zhang Jeffrey Tung Xi Zhang Dan Liu yingtian deng Zhendong Tian Huilan Chen Taotao Wang Weixiao Yin Bo Lij Zhibing Lai Savithramma P.Dinesh-Kumar Barbara Baker Feng Li National Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops College of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhanHubei 430070China Plant Gene Expression Center Department of Plant and Microbial BiologyUniversity of CaliforniaBerkeleyBerkeleyCA 94706USA College of Plant Science and Technology Huazhong Agricultural UniversityWuhanHubei 430070China College of Life Science and Technology Huazhong Agricultural UniversityWuhanHubei 430070China Department of Plant Biology and The Genome Center College of Biological SciencesUniversity of CalforniaDavisDavisCA 95616USA The Key Laboratory of Horticultural Plant Genetic and Improvement of Jiangxi Province Institute of Biological ResourcesJiangxi Academy of SciencesNanchang 330299China institute of Vegetables and Flowers Jiangxi Academy of Agricultural SciencesNanchang 330200Chin Hubei Hongshan Laboratory WuhanHubei 430070China

Nicotiana tabacum and Nicotiana benthamiana are widely used models in plant biology ***,genomic studies of these species have *** we report the chromosome-level reference genome assemblies for *** and *** with an estimated 99.5%and 99.8%completeness,*** transcription start and termination site sequencing methods were developed and used for accurate gene annotation in *** analyses revealed evidence for the parental origins and chromosome structural changes,leading to hybrid genome formation of each ***,theantiviral silencinggenesRDR1,RDR6,DCL2,DCL3,andAGO2were lost from one or both subgenomes in ***,while both homeologs were kept in ***,the *** genome encodes fewer immune receptors and signaling components than that of *** find-ings uncover possible reasons underlying the hypersusceptible nature of *** developed the user-friendly Nicomics(http:/***/Nicomics/)web server to facilitate better use of Nicotiana genomic resources as well as gene structure and expression analyses.

关键词： Nicotiana tabacum Nicotiana benthamiana genomics evolution disease resistance NLR

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Mitochondrial protein AtTIM9 and AtTIM10 are non-redundantly essential for early embryogenesis in Arabidopsis

Mitochondrial protein AtTIM9 and AtTIM10 are non-redundantly...

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2013全国植物生物学大会

作者： deng yingtian ZOU Wenxuan LI Gang ZHAO Jie State Key Laboratory of Hybrid Rice College of Life Sciences

Translocase of the inner membrane 9 and 10(TIM9 and TIM10),commonly known as a TIM9:10 complex,is an important component of the mitochondria import apparatus,and shuttle between the outer and inner membrane of ***,up to date,their function in plant development is still *** this study,we report functional characterization of AtTIM9 and AtTIMIO through analysis of their mutations,that all of tim9-1/+, tim9-2/+,and tim10/+ mutants display lethality of the homozygous embryos at the dermatogen stage which is arresting early embryo proper with 16/32 cells,and the double mutant tim9-1/+ tim10/+ showed the same *** AtTIM9 and AtTIM10 genes express ubiquitously throughout the plant tissues,including the developing embryo at each ***,it is verified that the AtTIM9 and AtTIM10 proteins have highly homologous among eukaryotes,and co-localize in mitochondria and can interact with each other as well by using yeast two-hybrid, Co-IP and *** using fluorescence and TEM as well as early embryo isolation techniques, it is revealed that the embryo proper cells but not suspensor in tim9-1 and tim10 mutants exhibit abnormal mitochondria which have mass decrease mitochondrial *** the same time,in these cells of tim9-1 and tim10,the change of mitochondrial membrane potential(△ψm) is happened,making mitochondrial dysfunction,and finally leading to the program cell ***,our findings suggest that AtTIM9 and AtTIM10 function identically and non-redundantly,and play essential roles in Arabidopsis early embryogenesis by controlling mitochondrial morphology and physiology to maintain its normal activity.

关键词： Arabidopsis AtTIM9 and AtTIM10 early embryogenesis mitochondria

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Non-SMC elements 1 and 3 are required for early embryo and seedling development in Arabidopsis

Non-SMC elements 1 and 3 are required for early embryo and s...

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2016年全国植物生物学大会

作者： Gang Li Wenxuan Zou Liufang Jian Jie Qian yingtian deng Jie Zhao State Key Laboratory of Hybrid Rice College of Life SciencesWuhan University

The structural maintenance of the chromosome 5/6(SMC5/6) complex has a variety of functions in mitosis and meiosis in yeast and human,including facilitating homologous recombination,restarting stalled replication forks,and maintaining ribosomal DNA and heterochromatin,among other roles. In Arabidopsis,At SMC5 and At SMC6A/6B are responsive to DNA damage. However,the non-SMC element 1 and 3 functions in Arabidopsis growth and development remain as yet unknown. In our study,it was found that the loss function of the non-SMC element 1 and 3 led to the conversion of suspensor cell fate and caused defects in embryo pattern formation. Partially-complemented homologous mutants showed that the post-embryonic development of the mutants was inhibited,that chromosome fragments occurred during segregation of chromosomes,and that the transition from the G2 phase to the M phase was delayed,leading to endoreduplication. All of these results demonstrate that the non-SMC element 1 and 3 are essential for cell division and function in maintaining the stability of chromosome ploidy in mitosis. Further,a large number of dead cells and DNA DSBs were observed in the their mutants,and the expression of the non-SMC element 1 and 3 were up-regulated following treatment of the plants with DSBs inducer compounds,indicating that the non-SMC element 1 and 3 play roles in DNA damage repair. Therefore,we conclude that the nonSMC element 1 and 3 facilitate DSBs repair and contribute to maintaining the stability of chromosome ploidy in mitotic cells. The non-SMC element 1 and 3 could thus be considered as required factors for maintaining proper early embryo cell differentiation and post-embryonic development.

关键词： non-SMC elements embryo seedling Arabidopsis

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PepperHub, an Informatics Hub for the Chili Pepper Research Community

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Molecular Plant 2017年第8期10卷 1129-1132页

作者： Feng Liu Huiyang Vu yingtian deng Jingyuan Zheng Minglei Liu Lijun Ou Bozhi Yang Xiongze Dai Yanqing Ma Shengyu Feng Shuang He Xuefeng Li Zhuqing Zhang Wenchao Chen Shudong Zhou Rong Chen Minmin Liu Sha Yang Ruimin Wei Huadong Li Feng Li Bo Ouyang Xuexiao Zou Institute of Vegetable Research Hunan Academy of Agricultural Science Changsha 410125 China Key Laboratory of Horticultural Plant Biology (Ministry of Education) Huazhong Agricultural University Wuhan 430070 China

Dear Editor Pepper belongs to the Solanaceae family, which includes many important vegetable crops such as tomato, potato, and eggplant. Not only widely used as vegetables and spicy ingredients, pepper also has diverse applications in pharmaceutics, natural coloring agents, cosmetics, defense repellents, and as ornamental plants （Kim et al., 2014; Qin et al., 2014）. Pepper is among the most widely cultivated and consumed vegetables in the world, with annual production reachincl to 38 million tons in 2011 （***.

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miRNA调控园艺作物生长发育研究进展

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园艺学报 2022年第5期49卷 1145-1161页

作者：张倩雯杨希航李峰邓颖天华中农业大学园艺林学学院园艺植物生物学教育部重点实验室武汉430070 剑桥大学植物科学系剑桥郡CB23EA英国

miRNA(microRNA)是真核生物中广泛存在的内源性非编码小RNA,通过碱基互补配对与靶基因的mRNA结合,切割靶mRNA或者抑制靶mRNA的翻译来抑制靶基因的表达。本研究综述了miRNA调控园艺作物生长发育的研究进展,重点阐述了miRNA在园艺作物根... 详细信息

miRNA(microRNA)是真核生物中广泛存在的内源性非编码小RNA,通过碱基互补配对与靶基因的mRNA结合,切割靶mRNA或者抑制靶mRNA的翻译来抑制靶基因的表达。本研究综述了miRNA调控园艺作物生长发育的研究进展,重点阐述了miRNA在园艺作物根、叶、花、果实等器官发育过程中的重要作用。

关键词：园艺作物 miRNA 调控作用生长发育研究进展

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Arabidopsis replication factor C4（AtRFC4） is critical for DNA replication during the mitotic cell cycle

Arabidopsis replication factor C4（AtRFC4） is critical for ...

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中国植物学会第十六次全国会员代表大会暨85周年学术年会

作者： Jie Qian Yueyue Chen Ying Hu yingtian deng Yang Liu Gang Li Wenxuan Zou Jie Zhao State Key Laboratory of Hybrid Rice College of Life SciencesWuhan University

Replication factor C（RFC） is a conserved eukaryotic complex consisting of RFC1/2/3/4/*** plays important roles in DNA replication and the cell cycle in yeast and fruit ***,it is not very clear how RFC subunits function in higher plants,except for the Arabidopsis（At） subunits AtRFC1 and *** this study,we investigated the functions of AtRFC4 and found that loss of function of AtRFC4 led to an early sporophyte lethality that initiated as early as the elongated zygote stage,all defective embryos arrested at the two-to four-cell embryo proper stage,and the endosperm possessed six to eight free *** of rfc4-1/+ with AtRFC4 expression driven through the embryo-specific DD45 pro and ABI3 pro or the endosperm-specific FIS2 pro could not completely restore the defective embryo or endosperm,whereas a combination of these three promoters in rfc4-1/+ enabled the aborted ovules to develop into viable *** suggests that AtRFC4 functions simultaneously in endosperm and embryo and that the proliferation of endosperm is critical for embryo *** of DNA content in rfc4-1/+ verified that DNA replication was disrupted in endosperm and embryo,resulting in blocked ***,we observed a decreased proportion of late S-phase and M-phase cells in the rfc4-1/–FIS2;DD45;ABI3 pro::AtRFC4 seedlings,suggesting that incomplete DNA replication triggered cell cycle arrest in cells of the root apical ***,we conclude that AtRFC4 is a crucial gene for DNA replication.

关键词： Arabidopsis thaliana replication factor C4 embryo endosperm DNA replication cell cycle

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Replication factor C1 (RFC1) is required for double-strand break repair during meiotic homologous recombination of Arabidopsis

Replication factor C1 (RFC1) is required for double-strand ...

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2012全国发育生物学大会

作者： Yang Liu,yingtian deng,Gang Li,Jie Zhao~* State Key Laboratory of Hybrid Rice,College of Life Sciences,Wuhan University,Wuhan 430072

Replication factor C1(RFC1),conserved in eukaryotes,is involved in DNA replication and checkpoint control. However,a RFC1 product participating in DNA repair at meiosis has not been reported in ***,we report a functional characterization of AtRFC1 through the analysis of the rfc1-2 *** rfc1-2 mutant displayed normal vegetative growth but had silique sterility because the male gametophyte was arrested at the uninucleus microspore stage and the female at the functional megaspore *** expression of AtRFC1 was concentrated in the reproductive organ primordia,meiocytes and developing *** spreading analysis showed that paring and synapsis were normal,and the chromosomes were broken when desynapsis began at late prophase I,and remained in the subsequent stages. For this reason,homologous chromosome and sister chromatid segregated unequally and thus leading to pollen sterility. Immunolocalization revealed that AtRFC1 foci localized to the chromosomes during zygotene and pachytene in wild-type but was absent in the atspoll-1 *** chromosome fragmentation of rfcl-2 could be suppressed by atspo11-1 indicating that AtRFC1 acted downstream of *** similar chromosome behavior of atrad51 rfc1-2 and atrad51 suggesting AtRFC1 may act with AtRAD51 in the same pathway. Conclusions:In summary,AtRFC1 was required for DNA double-strand break repair during meiotic homologous recombination of Arabidopsis.

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