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Neurotoxicity mechanism of aconitine in HT22 cells studied by microfluidic chip-mass spectrometry

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Journal of Pharmaceutical Analysis 2023年第1期13卷 88-98页

作者： yingrui Zhang Shiyu chen Fangfang Fan Ning Xu Xian-Li Meng Yi Zhang Jin-Ming Lin State Key Laboratory of Southwestern Chinese Medicine Resources School of PharmacyChengdu University of Traditional Chinese MedicineChengdu611137China Beijing Key Laboratory of Microanalytical Methods and Instrumentation Department of ChemistryTsinghua UniversityBeijing100084China Ethnic Medicine Academic Heritage Innovation Research Center Chengdu University of Traditional Chinese MedicineChengdu611137China

Aconitine,a common and main toxic component of Aconitum,is toxic to the central nervous ***,the mechanism of aconitine neurotoxicity is not yet *** this work,we had the hypothesis that excitatory amino acids can trigger excitotoxicity as a pointcut to explore the mechanism of neurotoxicity induced by ***22 cells were simulated by aconitine and the changes of target cell metabolites were real-time online investigated based on a microfluidic chip-mass spectrometry ***,to confirm the metabolic mechanism of aconitine toxicity on HT22 cells,the levels of lactate dehydrogenase,intracellular Ca^(2+),reactive oxygen species,glutathione and superoxide dismutase,and ratio of Bax/Bcl-2 protein were detected by molecular *** of the detected results revealed that neurotoxicity induced by aconitine was associated with the process of excitotoxicity caused by glutamic acid and aspartic acid,which was followed by the accumulation of lactic acid and reduction of *** surge of extracellular glutamic acid could further lead to a series of cascade reactions including intracellular Ca^(2+)overload and oxidative stress,and eventually result in cell *** general,we illustrated a new mechanism of aconitine neurotoxicity and presented a novel analysis strategy that real-time online monitoring of cell metabolites can provide a new approach to mechanism analysis.

关键词： Aconitine Neurotoxicity mechanism HT22 cells Excitatory amino acids Microfluidic chip-mass spectrometry

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中药药对在慢性肾脏病治疗中的研究进展

中医临床研究 2024年第9期16卷 68-72页

作者：陈星星吕冬宁李颖睿刘明敏广西中医药大学广西南宁530000 广西国际壮医医院广西南宁530000

慢性肾脏病(Chronic Kidney Disease,CKD)是由各种肾脏疾病发展而来的一组复杂的临床综合征。其病症复杂,并发症多。目前,西医治疗上有局限性,中医药在CKD的防治方面具有独特的优势。中医认为CKD的基本病机为正虚邪实。早期以脾肾气阴... 详细信息

慢性肾脏病(Chronic Kidney Disease,CKD)是由各种肾脏疾病发展而来的一组复杂的临床综合征。其病症复杂,并发症多。目前,西医治疗上有局限性,中医药在CKD的防治方面具有独特的优势。中医认为CKD的基本病机为正虚邪实。早期以脾肾气阴两虚为主,日久脾肾失司,水湿不化,导致湿浊阻滞、瘀血内停和浊毒内生,治疗以扶正补虚和利湿泻浊、化瘀通络并重,从而达到阴阳平衡,改善肾功能,延缓CKD进展的作用。CKD的主要治疗方法是运用中药复方。中药药对是中药复方的核心,它具有简单易用的优点,将中药的传统作用与现代药理作用相结合,使中药药对在治疗CKD中变得灵活精准,同时增加了病症的治疗范围和临床疗效。文章总结了近10年中医药在CKD治疗方面的常用药对,针对不同的中医证候采用不同药对治疗,按不同功效列举了益气养血类(黄芪-当归、黄芪-党参)、补益脾肾类(山茱萸-山药、金樱子-芡实、墨旱莲-女贞子)、活血通络类(蒲黄-五灵脂、三棱-莪术)、利水消肿类(茯苓-猪苓、防己-黄芪)等常用药对在治疗CKD中的临床应用及应用场景,分析中药药对的配伍特色,总结中药药对的传统功效及现代药理研究,为中药药对治疗CKD不同证型的应用提供参考。

关键词：中医药药对慢性肾脏病临床应用

陕西奶牛源微小隐孢子虫的基因亚型鉴定及其遗传进化分析

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家畜生态学报 2024年第10期45卷 72-76页

作者：张迎锐王占宏丰嘉怡赵永攀陈超李韬张智鹏陕西省汉中市勉县动物疫病预防控制中心陕西勉县724299 陕西省洋县动物疫病预防控制中心陕西洋县723300 西北农林科技大学动物医学院陕西杨凌712100 陕西省畜牧产业试验示范中心陕西泾阳713702 陕西省汉中市动物疫病预防控制中心陕西汉中723099

隐孢子虫是一种寄生于人和多种动物消化道的人兽共患寄生性原虫,可通过污染食物、水源等感染宿主,常引起宿主水样腹泻,甚至死亡。微小隐孢子虫(Cryptosporidium parvum)是隐孢子虫属中致病力较高的种类之一,有研究表明,***的致病力与其g... 详细信息

隐孢子虫是一种寄生于人和多种动物消化道的人兽共患寄生性原虫,可通过污染食物、水源等感染宿主,常引起宿主水样腹泻,甚至死亡。微小隐孢子虫(Cryptosporidium parvum)是隐孢子虫属中致病力较高的种类之一,有研究表明,***的致病力与其gp60基因亚型有强相关性。因此,本研究基于gp60基因位点并应用分子生物学方法对分离自陕西奶牛源微小隐孢子虫进行基因亚型鉴定和遗传进化分析。结果表明,该研究中奶牛源***分离株(SX-1)属于Ⅱd亚型家族,基因亚型为ⅡdA19G1,与GenBank中MH049734、MN304768、MT156342和AY738194等参考分离株同归于一个大支,均属于Ⅱd亚型家族。该研究所获得的结果表明***在陕西省奶牛中分布广泛且具有遗传多样性,对做好犊牛腹泻疾病的精准防控具有重要意义,可为陕西奶牛源***有效防控策略的制定提供理论基础和数据支撑。

关键词：微小隐孢子虫基因亚型遗传进化分析奶牛陕西

液体石蜡发酵生产琥珀酸的研究——Ⅰ.菌种的筛选和诱变

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微生物学报 1981年第2期 229-233页

作者：田静傅妙福徐纯锡林应锐王世卓陈兴吴徐冠珠陈丽琼中国科学院微生物研究所

通过对703株酵母菌的筛选,得到了三株产琥珀酸在1%以上的菌株,对其中的S19进行了诱变处理,从而得到了产琥珀酸最高的SB-7,该菌发酵液中琥珀酸含量达4.43%。发酵产物经分离提纯,得到无色结晶物质,经纸层析,熔点测定和1H 250 MHz核磁共振... 详细信息

通过对703株酵母菌的筛选,得到了三株产琥珀酸在1%以上的菌株,对其中的S19进行了诱变处理,从而得到了产琥珀酸最高的SB-7,该菌发酵液中琥珀酸含量达4.43%。发酵产物经分离提纯,得到无色结晶物质,经纸层析,熔点测定和1H 250 MHz核磁共振分析,证明是琥珀酸。

关键词：液体石蜡石油蜡发酵生产诱变发酵液纸层析法出发菌株原始菌株琥珀酸丁二酸菌种

Gastrodin modified polyurethane conduit promotes nerve repair via optimizing Schwann cells function

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Bioactive Materials 2022年第2期7卷 355-367页

作者： Hongcai Yang Qing Li Limei Li Shaochun chen Yu Zhao yingrui Hu Lu wang Xiaoqian Lan Lianmei Zhong Di Lu Yunnan Key Laboratory of Stem Cell and Regenerative Medicine Department of NeurologyThe First Affiliated HospitalKunming Medical UniversityKunming650500China Yunnan Key Laboratory of Stem Cell and Regenerative Medicine Science and Technology Achievement Incubation CenterKunming Medical UniversityKunming650500China The School of Rehabilitation Kunming Medical UniversityKunming650500China

Peripheral nerve regeneration and functional recovery remain a major clinical *** guidance conduit(NGC)that can regulate biological behavior of Schwann cells(SCs)and facilitate axonal regeneration through microenvironmental remodeling is beneficial for nerve regeneration and functional ***,a main constituent of a Chinese traditional herbal medicine,has been known to display several biological and pharmacological properties,especially antioxidative,anti-inflammatory and nerve ***,polyurethane(PU)NGCs modified by different weight ratio of Gastrodin(0,1 and 5 wt%)were designed for sequential and sustainable drug release,that created a favorable microenvironment for nerve *** scaffold showed suitable pore structure and biocompatibility in vitro,and evidently promoted morphological and functional recovery of regenerated sciatic nerves in *** to the PU and 1% Gastrodin/PU scaffolds,the 5% Gastrodin/PU significantly enhanced the proliferation,migration and myelination of SCs and up-regulated expression of neurotrophic factors,as well as induction of the differentiation of PC12 ***,the obvious anti-inflammatory response was observed in 5% Gastrodin/PU by reduced expression of TNF-α and iNOS,which also evidenced by the few fibrous capsule formation in the subcutaneous *** a construct presented a similarity to autograft in vivo repairing a 10 mm sciatic nerve *** was able to not only boost the regenerated area of nerve and microvascular network,but also facilitate functional axons growth and remyelination,leading to highly improved functional *** findings demonstrate that the 5% Gastrodin/PU NGC efficiently promotes nerve regeneration,indicating their potential for use in peripheral nerve regeneration applications.

关键词： Gastrodin/PU SCs biological behavior Anti-inflammatory Peripheral nerve regeneration

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鼻咽癌放射治疗后复发因素Cox模型评价

广东医学 1999年第6期20卷 417-418页

作者：曾子君谢松喜陈应瑞广东省人民医院放疗科 510080

目的　探讨鼻咽癌放射治疗后复发有关因素 ,为提高鼻咽癌的疗效 ,减少复发提供参考。方法　收集鼻咽癌复发病例 60例 ,对造成复发的可能因素进行Cox模型单因素及多因素分析。结果　年龄、TN分期、放射剂量及照射野与复发有关 ,T分期、... 详细信息

目的　探讨鼻咽癌放射治疗后复发有关因素 ,为提高鼻咽癌的疗效 ,减少复发提供参考。方法　收集鼻咽癌复发病例 60例 ,对造成复发的可能因素进行Cox模型单因素及多因素分析。结果　年龄、TN分期、放射剂量及照射野与复发有关 ,T分期、鼻咽剂量、照射野是复发主要因素。

关键词：鼻咽癌复发 Cox模型放射疗法

Observability Analysis of Composite Automata based on Algebraic State Space

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Observability Analysis of Composite Automata based on Algebr...

Highly Anisotropic Magnetism and Nearly Isotropic Magnetocaloric Effect in Mn_(3)Sn_(2)Single Crystals

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作者： yingrui Zhou Zengqiang chen Zhipeng Zhang Zhongxin Liu College of Artificial Intelligence Nankai University School of Computer Science and Engineering Tianjin University of Technology

Observability analysis of Discrete Event Systems（DESs） is a significant subject. In this paper, a matrix approach to verify four kinds of observability of partial-observed composite automata is proposed. Firstly, as a class of logic dynamic systems, DESs, modeled as finite automata, can be established its algebraic structure by semi-tensor product of matrices and due to the actual needs, we focus on composite automata formed by prallel composition. Then, aiming at initial state observability and current state observability with or without input information, we construct two polynomial matrices and present four necessary and sufficient theorems to verify the corresponding observability. Besides, all results obtained in this paper are applied to a practical example: the exhaust gas recirculation system.

关键词： Observability Composite Automata Semi-tensor Product of Matrices Logical Systems

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Chinese Physics Letters 2023年第12期40卷 149-154页

作者： Jianli Bai Qingxin Dong Libo Zhang Qiaoyu Liu Jingwen cheng Pinyu Liu Cundong Li yingrui Sun Yu Huang Zhian Ren Genfu chen Institute of Physics and Beijing National Laboratory for Condensed Matter Physics Chinese Academy of SciencesBeijing 100190China School of Physical Sciences University of Chinese Academy of SciencesBeijing 100049China Songshan Lake Materials Laboratory Dongguan 523808China

Mn_(3)Sn_(2)has been proposed as an ideal material for magnetic *** undergoes two successive ferromagnetic transitions(T_(C1)=262 K and T_(C2)=227 K)and one antiferromagnetic transition(TN=192 K).Herein we report,for the first time,the preparation of single crystals of Mn_(3)Sn_(2)from Bi *** resultant anisotropic magnetic properties and magnetocaloric effect are investigated along the three principal crystallographic directions of the *** anisotropy of magnetic susceptibility and multiple field-induced metamagnetic transitions were found at low fields,whereas the magnetocaloric effect was found to be almost isotropic and larger than that of the polycrystalline *** maximum magnetic entropy change amounts to-ΔSM=4.01 J·kg^(-1)·K^(-1)near T_(C1)under a magnetic field change of μ_(0)ΔH=5 T along the c-axis,with the corresponding refrigerant capacity of 1750 mJ·cm^(-3).Combined with a much wider cooling temperature span(~80 K),our results demonstrate Mn_(3)Sn_(2)single crystal to be an attractive candidate working material for active magnetic refrigeration at low temperatures.

关键词： transitions Crystal attractive

慢性牙周炎与慢性阻塞性肺疾病的潜在共同分子机制及其转录因子初探

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华西口腔医学杂志 2023年第5期41卷 533-540页

作者：张晨候珍珍宗颖睿郑州大学第一附属医院口腔预防科郑州450052

目的探讨慢性牙周炎(CP)和慢性阻塞性肺疾病(COPD)之间潜在的cross-talk基因、相关通路和转录因子。方法从GEO数据库下载CP(GSE10334和GSE16134)和COPD(GSE76925)的基因表达谱,进行差异表达分析、功能聚类分析、构建蛋白质相互作用(PPI... 详细信息

目的探讨慢性牙周炎(CP)和慢性阻塞性肺疾病(COPD)之间潜在的cross-talk基因、相关通路和转录因子。方法从GEO数据库下载CP(GSE10334和GSE16134)和COPD(GSE76925)的基因表达谱,进行差异表达分析、功能聚类分析、构建蛋白质相互作用(PPI)网络,通过4种拓扑分析算法和模块划分筛选出核心cross-talk基因,再进行功能聚类分析。结果GSE10334共筛选出164个差异表达基因(DEG)(119个上调、45个下调);GSE16134共筛选出208个DEG(154个上调、54个下调);GSE76925共筛选出1408个DEG(557个上调、851个下调)。PPI网络包括21个节点和20条边。最终筛选出7个核心基因CD79A、FCRLA、CD19、IRF4、CD27、SELL和CXCL13,相关通路为原发性免疫缺陷,B细胞受体信号通路和细胞因子-细胞因子受体相互作用。其得分最高的转录因子为IRF4。结论本研究探索了CP和COPD的潜在共同发病机制,其cross-talk基因、相关通路和转录因子可能为进一步的机制研究提供新思路。

关键词：慢性牙周炎慢性阻塞性肺疾病 cross-talk基因转录因子