Gastrodin modified polyurethane conduit promotes nerve repair via optimizing Schwann cells function

作     者：Hongcai Yang Qing Li Limei Li Shaochun Chen Yu Zhao Yingrui Hu Lu wang Xiaoqian Lan Lianmei Zhong Di Lu 

作者机构：Yunnan Key Laboratory of Stem Cell and Regenerative MedicineDepartment of NeurologyThe First Affiliated HospitalKunming Medical UniversityKunming650500China Yunnan Key Laboratory of Stem Cell and Regenerative MedicineScience and Technology Achievement Incubation CenterKunming Medical UniversityKunming650500China The School of RehabilitationKunming Medical UniversityKunming650500China 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2022年第7卷第2期

页      面：355-367页

核心收录：

学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(81960251/81460210/81860326) the Department of Science and Technology of Yunnan Province of China(2019ZF011-2/2017FF117(-062)/2018IA048/2018FE001(-029)/2018FE001(-024)) Scientific Research Fund of Yunnan Provincial Education Commission(2021J0229) the 100 Talents Program of Kunming Medical University(Limei Li) 

主　　题：Gastrodin/PU SCs biological behavior Anti-inflammatory Peripheral nerve regeneration 

摘      要：Peripheral nerve regeneration and functional recovery remain a major clinical *** guidance conduit(NGC)that can regulate biological behavior of Schwann cells(SCs)and facilitate axonal regeneration through microenvironmental remodeling is beneficial for nerve regeneration and functional ***,a main constituent of a Chinese traditional herbal medicine,has been known to display several biological and pharmacological properties,especially antioxidative,anti-inflammatory and nerve ***,polyurethane(PU)NGCs modified by different weight ratio of Gastrodin(0,1 and 5 wt%)were designed for sequential and sustainable drug release,that created a favorable microenvironment for nerve *** scaffold showed suitable pore structure and biocompatibility in vitro,and evidently promoted morphological and functional recovery of regenerated sciatic nerves in *** to the PU and 1% Gastrodin/PU scaffolds,the 5% Gastrodin/PU significantly enhanced the proliferation,migration and myelination of SCs and up-regulated expression of neurotrophic factors,as well as induction of the differentiation of PC12 ***,the obvious anti-inflammatory response was observed in 5% Gastrodin/PU by reduced expression of TNF-α and iNOS,which also evidenced by the few fibrous capsule formation in the subcutaneous *** a construct presented a similarity to autograft in vivo repairing a 10 mm sciatic nerve *** was able to not only boost the regenerated area of nerve and microvascular network,but also facilitate functional axons growth and remyelination,leading to highly improved functional *** findings demonstrate that the 5% Gastrodin/PU NGC efficiently promotes nerve regeneration,indicating their potential for use in peripheral nerve regeneration applications.