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Adenosine Diphosphate Ribosylation Factor-GTPase-Activating Protein Stimulates the Transport of AUX1 Endosome,Which Relies on Actin Cytoskeletal Organization in Rice Root Development

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Journal of Integrative Plant Biology 2011年第9期53卷 698-709页

作者： Cheng Du Yunyuan Xu yingdian wang Kang Chong Key Laboratory of Photosynthesis and Molecular Environmental Physiology Institute of Botanythe Chinese Academy of SciencesBeijing 100093China College of Life Sciences Beijing Normal UniversityBeijing 100875China

Polar auxin transport,which depends on polarized subcellular distribution of AUXIN RESISTANT 1/LIKE AUX1（AUX1/LAX） influx carriers and PIN-FORMED（PIN） efflux carriers,mediates various processes of plant growth and development.Endosomal recycling of PIN1 is mediated by an adenosine diphosphate（ADP）ribosylation factor（ARF）-GTPase exchange factor protein,GNOM.However,the mediation of auxin influx carrier recycling is poorly understood.Here,we report that overexpression of OsAGAP,an ARF-GTPase-activating protein in rice,stimulates vesicle transport from the plasma membrane to the Golgi apparatus in protoplasts and transgenic plants and induces the accumulation of early endosomes and AUX1.AUX1 endosomes could partially colocalize with FM4-64 labeled early endosome after actin disruption.Furthermore,OsAGAP is involved in actin cytoskeletal organization,and its overexpression tends to reduce the thickness and bundling of actin filaments.Fluorescence recovery after photobleaching analysis revealed exocytosis of the AUX1 recycling endosome was not affected in the OsAGAP overexpression cells,and was only slightly promoted when the actin filaments were completely disrupted by Lat B.Thus,we propose that AUX1 accumulation in the OsAGAP overexpression and actin disrupted cells may be due to the fact that endocytosis of the auxin influx carrier AUX1 early endosome was greatly promoted by actin cytoskeleton disruption.

关键词： actin adenosine diphosphate ribosylation factor-GTPase-activating protein AUX1 endosome polar auxin transport.

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A Bi-Functional Xyloglucan Galactosyltransferase Is an Indispensable Salt Stress Tolerance Determinant in Arabidopsis

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Molecular Plant 2013年第4期6卷 1344-1354页

作者： Wenbo Li Qingmei Guan Zhen-Yu wang yingdian wang Jianhua Zhu College of Life Sciences Beijing Normal University Beijing 100875 China Department of Plant Science and Landscape Architecture University of Maryland College Park MD 20742 USA Center for Plant Stress Genomics and Technology King Abdullah University of Science and Technology Thuwal 23955-6900 Kingdom of Saudi Arabia

Salinity is an abiotic stress that substantially limits crop production worldwide. To identify salt stress tolerance determinants, we screened for Arabidopsis mutants that are hypersensitive to salt stress and designated these mutants as short root in salt medium （rsa）. One of these mutants, rsa3-1, is hypersensitive to NaCI and LiCI but not to CsCI or to general osmotic stress. Reactive oxygen species （ROS） over-accumulate in rsa3-1 plants under salt stress. Gene expression profiling with Affymetrix microarray analysis revealed that RSA3 controls expression of many genes including genes encoding proteins for ROS detoxification under salt stress. Map-based cloning showed that RSA3 encodes a xyloglucan galactosyltransferase, which is allelic to a gene previously named MUR3/KAM1. The RSA3/ MUR3/KAMl-encoded xylogluscan galactosyltransferase regulates actin microfilament organization （and thereby con- tributes to endomembrane distribution） and is also involved in cell wall biosynthesis. In rsa3-1, actin cannot assemble and form bundles as it does in the wild-type but instead aggregates in the cytoplasm. Furthermore, addition of phal- Ioidin, which prevents actin depolymerization, can rescue salt hypersensitivity of rsa3-1. Together, these results sug- gest that RSA3/MUR3/KAM1 along with other cell wall-associated proteins plays a critical role in salt stress tolerance by maintaining the proper organization of actin microfilaments in order to minimize damage caused by excessive ROS.

关键词： salt stress tolerance xyloglucan galactosyltransferase endomembranes actin microfilaments RSA3.

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Arabidopsis Synaptotagmin 2 Participates in Pollen Germination and Tube Growth and Is Delivered to Plasma Membrane via Conventional Secretion

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Molecular Plant 2015年第12期8卷 1737-1750页

作者： Hui wang Shengcheng Han Wei Siao Chunqing Song Yun Xiang Xiaorong Wu Pengyu Cheng Hongjuan Li JAn Jasik Karol Micieta JAn Turna Boris Voigtt Frantisek Baluska Jin Liu yingdian wang Heping Zhao Beijing Key Laboratory of Gene Resource and Molecular Development College of Life Science Beijing Normal University Beijing 100875 China Department of Plant Cell Biology IZMB University of Bonn Kirschallee 153115 Bonn Germany School of Life Science Lanzhou University Lanzhou 730000 China Comenius University Science Park Comenius University Bratislava Mlynska dolina 842 15 Bratislava 4 Slovakia Department of Botany Faculty of Natural Sciences Comenius University Revova 39 811 02 Bratislava 1 SIovakia Department of Botany Faculty of Natural Sciences Comenius University Revova 39 811 02 Bratislava 1 Slovakia Institute of Botany Slovak Academy of Sciences Dubravska cesta 9 SK-84523 Bratislava Slovak Republic These authors contributed equally to this article. present address: Office of Teaching Affairs Handan University Handan 056005 China

Arabidopsis synaptotagmin 2 （SYT2） has been reported to participate in an unconventional secretory pathway in somatic cells. Our results showed that SYT2 was expressed mainly in the pollen ofArabidopsis thaliana. The pollen of syt2 T-DNA and RNA interference mutant lines exhibited reduced total germination and impeded pollen tube growth. Analysis of the expression of SYT2-GFP fusion protein in the pollen tube indicates that SYT2 was localized to distinct, patchy compartments but could co-localize with the Golgi markers, BODIPY TR C5 ceramide and GmManl-mCherry. However, SYT2-DsRed-E5 was localized to the plasma membrane in Arabidopsis suspension cells, in addition to the Golgi apparatus. The localization of SYT2 at the plasma membrane was further supported by immunofluorescence staining in pollen tubes. Moreover, brefeldin A treatment inhibited the transport of SYT2 to the plasma membrane and caused SYT2 to aggregate and form enlarged compartments. Truncation of the SYT2-C2AB domains also resulted in retention of SYT2 in the Golgi apparatus. An in vitro phospholipid-binding assay showed that SYT2-C2AB domains bind to the phospholipid membrane in a calcium-dependent manner. Take together, our results indicated that SYT2 was required for pollen germination and pollen tube growth, and was involved in conventional exocytosis.

关键词： synaptotagmins pollen germination calcium-dependent phospholipid binding C2 domains conventional secretion

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牛粪尿短期储存氨释放与养分转化的抑制剂作用成效研究

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生态科学 2023年第5期42卷 1-11页

作者：陈哲吕艳花杜岩功张莉周华坤王英典王文颖青海师范大学/高原科学与可持续发展研究院西宁810016 青海省青藏高原生物多样性形成机制与综合利用重点实验室西宁810016 中国科学院西北高原生物研究所/青海省寒区恢复生态学重点实验室西宁810008

削减粪尿储存过程中氨(NH_(3))排放与氮素(N)损耗,是减少养殖业废弃物排放大气污染物和资源高效利用的重要举措。以青藏高原集约化养殖过程中新鲜牛粪尿混合物为研究对象,通过向粪尿中添加脲酶抑制剂(正丁基硫代磷酸三胺,NBPT)、硝化抑... 详细信息

削减粪尿储存过程中氨(NH_(3))排放与氮素(N)损耗,是减少养殖业废弃物排放大气污染物和资源高效利用的重要举措。以青藏高原集约化养殖过程中新鲜牛粪尿混合物为研究对象,通过向粪尿中添加脲酶抑制剂(正丁基硫代磷酸三胺,NBPT)、硝化抑制剂(3.4-二甲基吡唑磷酸盐,DMPP)及明矾,测定不同抑制剂添加后短期储存过程中(22天)粪尿NH_(3)释放量及不同形态养分含量,并利用结构方程模型分析影响粪尿储存过程中NH_(3)排放和N转化的各因素效应。结果表明:(1)DMPP和明矾处理下NH_(3)累积排放量及氮素损失量均与对照组无差异,而NBPT处理比对照组NH_(3)累积排放量降低25.07%;(2)NBPT处理使粪尿短期储存过程中总有机氮含量平均值较对照增加43.86%,而铵态氮含量下降39.15%,同时粪尿C:N平均值比对照增加20.99%;(3)粪肥储存期间高含水量有利于削减NH_(3)排放,脲酶抑制剂通过降低总有机氮分解和C:N,进而减少铵态氮生产并降低粪尿短期储存中NH_(3)逸散。总之,脲酶抑制剂能够通过控制铵的生成有效减缓牛粪尿混合物储存过程中N的损耗和NH_(3)释放,而硝化抑制剂及明矾的最优施用条件有待深入研究。

关键词：养殖废弃物氨挥发氮损失脲酶抑制剂硝化抑制剂明矾

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水稻颖果总RNA提取方法的研究

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北京师范大学学报（自然科学版） 2005年第1期41卷 79-81页

作者：陈星汪琦黄迎春王欣生王英典北京师范大学生命科学学院北京100875

利用TRIZOL试剂快速提取法、异硫氰酸胍法和改良的SDS法提取不同发育时期水稻颖果的总RNA,并通过凝胶电泳、紫外分光光度法检测提取获得的RNA样品的品质.研究结果表明,改良的SDS法提取的RNA具有28S rRNA和18SrRNA2条清晰的条带,且... 详细信息

利用TRIZOL试剂快速提取法、异硫氰酸胍法和改良的SDS法提取不同发育时期水稻颖果的总RNA,并通过凝胶电泳、紫外分光光度法检测提取获得的RNA样品的品质.研究结果表明,改良的SDS法提取的RNA具有28S rRNA和18SrRNA2条清晰的条带,且无降解.其D260/D280为1.84～1.97,具有较高的纯度.其他2种方法获得的RNA 品质较差,有降解和弥散现象.将改良的SDS法提取的RNA逆转录成cDNA,作为PCR扩增的模板,可获得清晰的约 1.7kb的目的条带.该法提取的RNA用于Northern杂交,可产生特异的杂交信号.这些结果进一步证明了改良的SDS 法提取的RNA具有很高的品质与纯度,可以满足下一步分子生物学研究的需要.

关键词：水稻颖果总RNA 提取

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基于绩效管理的高校实验技术队伍建设

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实验技术与管理 2011年第3期 342-345页

作者：叶伟萍王英典北京师范大学生命科学学院

如何建设一支管理到位、能力强、素质高、结构优化的实验技术队伍,已经成为当前高校建设一流大学和一流学科、实现全面可持续发展所必须解决的重要问题。介绍了北京师范大学生命科学学院针对实验技术队伍建设与管理过程中遇到的各类困... 详细信息

如何建设一支管理到位、能力强、素质高、结构优化的实验技术队伍,已经成为当前高校建设一流大学和一流学科、实现全面可持续发展所必须解决的重要问题。介绍了北京师范大学生命科学学院针对实验技术队伍建设与管理过程中遇到的各类困难和典型问题,在实践中探索解决之道。

关键词：实验技术队伍队伍建设绩效管理

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生命科学与生物技术实验教学示范中心的建设与实践

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实验技术与管理 2010年第3期 260-262页

作者：向本琼王英典刘进北京师范大学生命科学学院

结合生命科学与技术实验教学中心的建设与实践,介绍了中心的实践教学理念、教学体系与内容的改革、实践教学与管理平台的建设,为国内同类实验教学中心的建设提供参考。

关键词：示范中心教学改革实践教学

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Cloning and Expression Analysis of the Hexokinase Family in the Developing Spikelets of Rice (Oryza sativa L.)

Cloning and Expression Analysis of the Hexokinase Family in ...

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The Second International Symposium on Plant Neurobiology

作者： Wei Cheng, Xiaojin Zhou, Jie Li, Chao Chen, yingdian wang Laboratory of Plant Development Physiology and Molecular Biology, College of Life Sciences, Beijing Normal University, 19XinJieKouWai Avenue, Beijing 100875, China

The carbon metabolism and sugar signal transduction is critical in the plant growth and development processes. Plant hexokinase not only catalyzes the production of hexose 6-phosphates but also acts as a sugar sensor plays an important role in sugar sensing and signaling (Jang et al. 1997: Coruzzi and Bush, 2001;Moore et al.,2003).

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关于建立高校大型仪器设备绩效管理系统的思考

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实验技术与管理 2009年第6期 234-236页

作者：叶伟萍刘建武王英典北京师范大学生命科学学院

大型仪器设备绩效管理系统作为一项全新的管理手段,很好地实现了高校实验室中专业技术人员与仪器设备使用管理的统一,从而真正实现大型仪器设备的共享。该文介绍了北京师范大学生命科学学院针对大型仪器设备绩效管理系统建立过程中遇到... 详细信息

大型仪器设备绩效管理系统作为一项全新的管理手段,很好地实现了高校实验室中专业技术人员与仪器设备使用管理的统一,从而真正实现大型仪器设备的共享。该文介绍了北京师范大学生命科学学院针对大型仪器设备绩效管理系统建立过程中遇到的各类困难和典型问题,在实践中探索解决之道。

关键词：大型仪器设备绩效管理高校

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Effects of-NO2 and-NH2 functional groups in mixed-linker Zr-based MOFs on gas adsorption of CO2 and CH4

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Progress in Natural Science:Materials International 2018年第2期28卷 160-167页

作者： Zana Hassan Rada Hussein Rasool Abid Hongqi Sun Jin Shang Jiaye Li yingdian He Shaomin Liu Shaobin wang Department of Chemical Engineering Curtin University Department of Environmental Health Applied Medical Science College Karbala University School of Engineering Edith Cowan University School of Energy and Environment City University of Hong Kong Department of Chemistry Monash University Department of Chemical and Biomolecular Engineering The University of Melbourne

This study was undertaken to evaluate the effects of mixing BDC-NO2and BDC-NH2linkers in the synthesis of Zrbased metal organic frameworks(Zr-MOFs) on their adsorption and separation of CO2and CH4. Ui O-66 with single or binary-NO2and-NH2 samples were synthesized under solvothermal conditions and activated by solvent exchanging using methanol. Structural analyses of the materials were conducted using FTIR, XRD, TGA,SEM,1 HNMR and N2 adsorption/desorption techniques and adsorption of CO2and CH4at high pressures and different temperatures(273 and 298 K) was investigated. It was found that Ui O-66-NH2 exhibited higher CO2and CH4adsorption capacities than those of Ui O-66-NO2. Addition of-NH2functional group in Ui O-66-NO2could enhance CO2and CH4adsorption due to the extra CO2adsorption sites of-NH2functional groups. Addition of-NO2functional group to Ui O-66-NH2 at a low loading could also increase CO2and CH4adsorption, however,a high loading of NO2functional group to Ui O-66-NH2 would result in decreased adsorption.

关键词： UiO-66–NO2 UiO-66-NH2 Multi-linker MOFs Carbon dioxide Methane adsorption

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