A Bi-Functional Xyloglucan Galactosyltransferase Is an Indispensable Salt Stress Tolerance Determinant in Arabidopsis
A Bi-Functional Xyloglucan Galactosyltransferase Is an Indispensable Salt Stress Tolerance Determinant in Arabidopsis作者机构：College of Life Sciences Beijing Normal University Beijing 100875 China Department of Plant Science and Landscape Architecture University of Maryland College Park MD 20742 USA Center for Plant Stress Genomics and Technology King Abdullah University of Science and Technology Thuwal 23955-6900 Kingdom of Saudi Arabia
出 版 物：《Molecular Plant》 (分子植物（英文版）)
年 卷 期：2013年第6卷第4期
页 面：1344-1354页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0903[农学-农业资源与环境] 0901[农学-作物学] 090102[农学-作物遗传育种]
主 题：salt stress tolerance xyloglucan galactosyltransferase endomembranes actin microfilaments RSA3.
摘 要：Salinity is an abiotic stress that substantially limits crop production worldwide. To identify salt stress tolerance determinants, we screened for Arabidopsis mutants that are hypersensitive to salt stress and designated these mutants as short root in salt medium （rsa）. One of these mutants, rsa3-1, is hypersensitive to NaCI and LiCI but not to CsCI or to general osmotic stress. Reactive oxygen species （ROS） over-accumulate in rsa3-1 plants under salt stress. Gene expression profiling with Affymetrix microarray analysis revealed that RSA3 controls expression of many genes including genes encoding proteins for ROS detoxification under salt stress. Map-based cloning showed that RSA3 encodes a xyloglucan galactosyltransferase, which is allelic to a gene previously named MUR3/KAM1. The RSA3/ MUR3/KAMl-encoded xylogluscan galactosyltransferase regulates actin microfilament organization （and thereby con- tributes to endomembrane distribution） and is also involved in cell wall biosynthesis. In rsa3-1, actin cannot assemble and form bundles as it does in the wild-type but instead aggregates in the cytoplasm. Furthermore, addition of phal- Ioidin, which prevents actin depolymerization, can rescue salt hypersensitivity of rsa3-1. Together, these results sug- gest that RSA3/MUR3/KAM1 along with other cell wall-associated proteins plays a critical role in salt stress tolerance by maintaining the proper organization of actin microfilaments in order to minimize damage caused by excessive ROS.
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