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Zinc-deficient diet aggravates ventilation-induced lung injury in rats

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The Journal of Biomedical Research 2012年第1期26卷 59-65页

作者： Xiaoyu Chen Jieyu Bian yingbin ge Department of Pediatrics the First Affiliated Hospital to Nanjing Medical University Nanjing Jiangsu 210029 China Department of Physiology Nanjing Medical University Nanjing Jiangsu 210029 China

We investigated the effects of zinc deficiency on acute lung injury （ALI） induced by mechanical ventilation. Male Sprague-Dawley rats were fed with a zinc-deficient or zinc-proficient diet for 4 weeks, and then received mechanical ventilation at normal frequency and pressure for 30 min. Total protein, cell count, the number of poly- morphonuclear neutrophil （PMN） in the bronchoalveolar lavage （BAL）, and vascular endothelial growth factor （VEGF） expression in the lung were determined. Activation of nuclear factor-t^B （NF-~cB） was detected by exam- ining the phosphorylation of NF-kB （pNF-kB p65） and the expression of inhibitor of NF-kB （pI-kBa）. Compared to the controls, total cell count and the number of PMNs were significantly increased to 160% and 140%, respec- tively, in zinc-deficient rats treated with ventilation. Activation of NF-kB was significantly increased and VEGF was also increased to three-folds. Zinc deficiency aggravated the inflammatory response in rats and was associated with the overexpression of VEGF in response to mechanical ventilation. Zinc supplementation may be beneficial to zinc-deficient patients during mechanical ventilation.

关键词： ventilation lung injury zinc deficient nuclear factor-kB (NF-kB) vascular endothelial growth factor (VEGF) rat

Proliferation in rat gastric mucosal cells induced by chronic ethanol feeding through the ROS/BMK1 pathway

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Journal of Medical Colleges of PLA(China) 2009年第6期24卷 321-328页

作者： Fan Lingling ge yingbin Du Jun Li Yingchun Medical College Henan University of Science and Technology Luoyang 471003 China Department of Physiology Nanjing Medical University Nanjing 210029 China

Objective: To investigate the correlation between the gastric mucosal cell proliferation and low-concentration alcohol intake in a chronic drinking rat model, and to investigate the possible role of ROS/BMK1 pathway in this process. Methods: SD rats were randomly divided into 4 groups: control group, administered with tap water; ethanol group, with 6% ethanol in the drinking water; quercetin group, with quercetin (100 mg/kg) by intragastric gavage twice a day; ethanol+quercetin group, administered with quercetin combined with 6% ethanol. The cell proliferation in rat gastric mucosa was analyzed by flow cytometery and proliferating cell nuclear antigen (PCNA) immunohistochemical staining. Activation of ERKs and BMK1 was evaluated by the expression and phosphorylation of these kinases using Western Blot analysis. Results: Compared to the controls, the cell proliferation in gastric mucosa of rats exposed to the ethanol for 7 d was enhanced, and the activation of BMK1 was also increased in this period. Otherwise quercetin, as a free radical scavenger, attenuated increased cell proliferation and activation of BMK1 in rat stomach treated with ethanol. However, no changes of ERKs expression and phosphorylation occurred in the rats in all groups. Conclusion: These results suggested that the ROS and BMK1 activation may be a central mechanism, which underlies cell proliferation in rat gastric mucosa stimulus with the chronic low-concentration ethanol.

关键词： Alcohol Stomach Cell proliferation Big mitogen-activated protein kinases-1 Reactive oxygen species

Induction of metallothionein expression during monocyte to melanoma-associated macrophage differentiation

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Frontiers in Biology 2012年第4期7卷 359-367页

作者： yingbin ge Rikka AZUMA Bethsebah geKONge Alfonso LOPEZ-CORAL Min XIAO Gao ZHANG Xiaowei XU Luis J. MONTANER Zhi WEI Meenhard HERLYN Tao WANG Russel E. KAUFMAN Molecular and Cellular Oncogenesis Program The Wistar Institute Philadelphia PA 19104 USA Department of Physiology Nanjing Medical University Nanjing 210029 China Undergraduate Program University of Pennsylvania Philadelphia PA 19104 USA Tumor Microenvironment and Metastasis Program The Wistar Institute Philadelphia PA 19104 USA Graduate Program The Catholic University of America Washington DC 20064 USA Department of Pathology and Laboratory Medicine University of Pennsylvania Philadelphia PA 19104 USA Department of Computer Science New Jersey Institute of Technology NJ 07102 USA

Tumor-associated macrophages （TAMs） play a critical role in melanoma growth and metastasis. Infiltration of TAMs correlates with the poor prognosis of melanoma. TAMs are differentiated from monocytes in response to the tumor microenvironment cue. However, the mechanism how TAMs adapt to the tumor microenvironment after differentiation from monocytes is not fully understood. In addition, specific identification of TAMs in melanoma is difficult because the expression of the most commonly used macrophage marker, CD68, is also expressed in melanoma cells. In an earlier study, we found by gene microarray analysis that seven members of the metallothionein （MTs） family were upregulated in melanoma-conditioned medium induced macrophages （MCIM-MO）. MTs have been implicated in zinc metabolism and inflammation. In the present study, we confirmed that expression of metallothionein is induced in M-CSF differentiated macrophages （M-CSF/M~） and MCIM-M~ at both the mRNA and protein levels using real-time PCR, immunofluorescence, and western blot analysis. Furthermore, we demonstrated the presence of metallothionein in melanoma tissues in vivo and that metallothionein was co-localized with TAMs markers, CD68 and CD163. Finally, we demonstrated the induction of the zinc importer gene Zip8 both in M-CSF/MO and MCIM-MO. Our study identifies metallothionein as a novel marker for TAMs and suggests that metaliothionein might play important roles in macrophage adaptation and function in the tumor microenvironment.

关键词： melanoma macrophages metallothionein

他克莫司刺激星形胶质细胞分泌EGF促进神经细胞突起生长的研究

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中国修复重建外科杂志 2015年第11期29卷 1423-1428页

作者：蔡俊孙钰戈应滨曹晓建南京医科大学第一附属医院骨科南京210029 南京医科大学生理教研室

目的研究星形胶质细胞分泌的EGF在他克莫司(FK506)促进神经细胞突起生长过程中的作用。方法取2日龄SD大鼠和孕15 d SD大鼠,分别体外分离培养脊髓星形胶质细胞和脊髓神经元细胞,分别进行免疫荧光染色鉴定。将脊髓星形胶质细胞分为两组,... 详细信息

目的研究星形胶质细胞分泌的EGF在他克莫司(FK506)促进神经细胞突起生长过程中的作用。方法取2日龄SD大鼠和孕15 d SD大鼠,分别体外分离培养脊髓星形胶质细胞和脊髓神经元细胞,分别进行免疫荧光染色鉴定。将脊髓星形胶质细胞分为两组,实验组采用20μmol/L FK506培养液培养,对照组用不含FK506的培养液培养,24 h后收集上清制备条件培养液和细胞,分别行ELISA检查和实时荧光定量PCR(real-time quantitative PCR,RT-q PCR)检测EGF蛋白和基因表达。分别使用实验组和对照组条件培养液(分别为A、B组)和加入EGF中和抗体中和后的对照组和实验组条件培养液(分别为C、D组)培养脊髓神经元细胞,3 d后对脊髓神经元细胞的突起长度进行测量,比较神经突总长度和最长神经突长度。结果经免疫荧光染色鉴定,所培养细胞分别为脊髓星形胶质细胞和神经元细胞。ELISA检测示,实验组EGF含量为0.241±0.044,显著高于对照组的0.166±0.014(t=3.93,P=0.01);RT-q PCR检测得到了相同结果,实验组EGF基因相对表达量为1.12±0.25,显著高于对照组的0.46±0.11(t=5.78,P=0.00)。神经突长度测量显示,C、D组神经突总长度和最长神经突长度均显著小于A、B组(P0.05)。结论脊髓星形胶质细胞分泌的EGF能够促进神经元细胞突起的生长,可作为FK506促进神经功能恢复的一个重要中介靶点。

关键词：他克莫司脊髓神经元细胞星形胶质细胞 EGF 大鼠

Aggregation-enabled alkene insertion into carbon–halogen bonds

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Aggregate

Aggregate 2023年第5期4卷 128-136页

作者： Meng-Yao Li Xiao-Mei Nong Han Xiao Ao Gu Shuyang Zhai Jiatong Li ge Zhang Ze-Jian Xue yingbin Liu Chunsen Li Guo-Qiang Lin Chen-Guo Feng Shanghai Cancer Institute Department of Biliary-Pancreatic SurgeryRenji Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghaiChina The Research Center of Chiral Drugs Innovation Research Institute of Traditional Chinese MedicineShanghai Frontiers Science Center for Traditional Chinese Medicine Chemical BiologyShanghai University of Traditional Chinese MedicineShanghaiChina State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of MatterChinese Academy of SciencesFuzhouChina Shanghai Institute of Organic Chemistry Chinese Academy of SciencesShanghaiChina

Molecular aggregation affects the electronic interactions between molecules and has emerged as a powerful tool in material *** effect finds wide applications in the research of new physical phenomena;however,its value for chemical reaction development has been far less ***,we report the development of aggregation-enabled alkene insertion into carbon–halogen *** spontaneous cleavage of C–X(X=Cl,Br,or I)bonds generates an intimate ion pair,which can be quickly captured by alkenes in an aggregated *** catalysts or promoters are not necessary under such circumstances,and solvent quenching experiments indicate that the aggregated state is critical for achieving such *** ionic insertion mode is supported by mechanistic studies,density functional theory calculations,and symmetry-adapted perturbation theory *** also show that the non-aggregated state may quench the transition state and terminate the insertion process.

关键词： aggregated state chemistry atom-and step-economy difunctionalization of alkenes ionic insertion solvent-free and catalyst-free

Combination therapy with oncolytic virus and T cells or mRNA vaccine amplifies antitumor effects

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Signal Transduction and Targeted Therapy 2024年第6期9卷 2655-2668页

作者： Rao Fu Ruoyao Qi Hualong Xiong Xing Lei Yao Jiang Jinhang He Feng Chen Liang Zhang Dekui Qiu Yiyi Chen Meifeng Nie Xueran Guo Yuhe Zhu Jinlei Zhang Mingxi Yue Jiali Cao Guosong Wang Yuqiong Que Mujing Fang yingbin Wang Yixin Chen Tong Cheng Shengxiang ge Jun Zhang Quan Yuan Tianying Zhang Ningshao Xia State Key Laboratory of Vaccines for Infectious Diseases Xiang An Biomedicine LaboratoryDepartment of Laboratory MedicineSchool of Public Health&School of Life SciencesXiamen UniversityXiamen 361102 FujianChina National Institute of Diagnostics and Vaccine Development in Infectious Diseases Xiamen 361102 FujianChina

Antitumor therapies based on adoptively transferred T cells or oncolytic viruses have made significant progress in recent years,but the limited efficiency of their infiltration into solid tumors makes it difficult to achieve desired antitumor effects when used *** this study,an oncolytic virus(rVSV-LCMVG)that is not prone to induce virus-neutralizing antibodies was designed and combined with adoptively transferred T *** transforming the immunosuppressive tumor microenvironment into an immunosensitive one,in B16 tumor-bearing mice,combination therapy showed superior antitumor effects than *** occurred whether the OV was administered intratumorally or *** therapy significantly increased cytokine and chemokine levels within tumors and recruited CD8^(+)T cells to the TME to trigger antitumor immune *** with adoptively transferred T cells and subsequent oncolytic virotherapy sensitizes refractory tumors by boosting T-cell recruitment,downregulating the expression of PD-1,and restoring effector T-cell *** offer a combination therapy with greater translational value,mRNA vaccines were introduced to induce tumor-specific T cells instead of adoptively transferred T *** combination of OVs and mRNA vaccine also displays a significant reduction in tumor burden and prolonged *** study proposed a rational combination therapy of OVs with adoptive T-cell transfer or mRNA vaccines encoding tumor-associated antigens,in terms of synergistic efficacy and mechanism.

关键词： transferred rational boosting

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电阻率成像法监测人工梭梭林土壤水分

农业工程学报 2018年第22期34卷 92-101页

作者：高君亮罗凤敏马迎宾张格郝玉光内蒙古磴口荒漠生态系统国家定位观测研究站/中国林业科学研究院沙漠林业实验中心磴口015200

土壤水分是影响干旱半干旱沙区植物生长发育的主要限制因素。快速、准确地监测土壤水分时空动态可为干旱半干旱区植被建设与生态恢复提供科学依据。以乌兰布和沙漠东北部人工梭梭固沙林土壤为研究对象,在林内、外(根际、冠中、冠缘、行... 详细信息

土壤水分是影响干旱半干旱沙区植物生长发育的主要限制因素。快速、准确地监测土壤水分时空动态可为干旱半干旱区植被建设与生态恢复提供科学依据。以乌兰布和沙漠东北部人工梭梭固沙林土壤为研究对象,在林内、外(根际、冠中、冠缘、行间、林外)设置了5条监测样线,分别于一次强降雨后的第2天、第15天、第55天用多电极电阻仪定位测定了土壤电阻率,同步采取土样用烘干法测定了土壤实际含水率,建立了土壤含水率与土壤电阻率之间的相关关系,并对二维剖面土壤水分空间分布特征进行了分析。结果表明:1)土壤含水率与土壤电阻率之间为极显著负相关关系(P<0.01),可用幂函数表示。2)5条测线的土壤电阻率在3次监测时均随土层深度增加而减小,而土壤含水量随土层深度增加而增大,根际<冠中<冠缘<行间<林外。强降水后的不同时间内,由于受土壤属性、树冠对水分再分配、树干径流、根系吸收水分等影响,二维剖面上土壤水分空间分布格局有明显变化。随着雨后干旱时间的延长,0～51 cm水分含量由于受蒸发、植物吸收利用的影响而明显降低。3)电阻率成像技术在野外能快速准确,长期定位监测土壤水分含量;对地表扰动小,实现了非破坏性测量;保证测定精度的同时,还能提供尺度较大的土壤水分空间分布的详实数据,可高效快速地获取连续的土壤水分分布信息。

关键词：土壤水分降水土壤电阻率电阻率成像土壤蒸发根系吸水乌兰布和沙漠

心脏瓣膜术后死亡危险因素研究:倾向性评分匹配分析

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第三军医大学学报 2021年第3期43卷 244-248页

作者：葛开恒刘凌超朱雨肖颖彬陆军军医大学(第三军医大学)第二附属医院全军心血管外科研究所重庆400037

目的探讨心脏瓣膜手术后患者死亡的危险因素,指导围术期治疗。方法收集我科2016年9月至2019年8月行心脏瓣膜手术患者资料,将术后死亡患者50例纳入死亡组(已排除3例资料不全患者),另从4078例心脏瓣膜手术治愈患者中随机选择150例治疗患... 详细信息

目的探讨心脏瓣膜手术后患者死亡的危险因素,指导围术期治疗。方法收集我科2016年9月至2019年8月行心脏瓣膜手术患者资料,将术后死亡患者50例纳入死亡组(已排除3例资料不全患者),另从4078例心脏瓣膜手术治愈患者中随机选择150例治疗患者纳入对照组。采用倾向性评分匹配方法对两组患者(死亡组、对照组)进行配对,比较两组患者术中失血量、术中肝素用量、术中最低体温、主动脉球囊反搏(intra-aortic ballon pump,IABP)、手术过程、瓣膜类型、术后血气分析结果等临床特征,分析心脏瓣膜术后患者死亡危险因素,并绘制受试者工作(ROC)曲线。结果通过倾向性评分匹配出46对患者,死亡组失血量、IABP植入率、术后二氧化碳分压、术后乳酸均高于对照组(P<0.05),死亡组术后氧分压、术后红细胞比容、术后血氧饱和度低于对照组(P<0.05),Logistic回归分析结果显示二氧化碳分压升高、高乳酸血症是心脏瓣膜术后患者死亡危险因素(P<0.05),术后乳酸及二氧化碳分压预测死亡的ROC曲线下面积分别为0.918、0.793,术后乳酸与二氧化碳分压的临界值分别为7.55 mmol/L、49.50 mmHg。结论高乳酸血症及二氧化碳分压升高是心脏瓣膜术后死亡的危险因素,术后治疗应该维持充足氧供,改善组织灌注状况以降低死亡风险。

关键词：倾向性评分匹配瓣膜手术死亡危险因素

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黑木耳糖蛋白的提取及单糖、氨基酸组成分析

食品工业科技 2023年第13期44卷 197-206页

作者：王映斌张煜李星那治国黑龙江东方学院食品工程学院黑龙江哈尔滨150066 黑龙江省林业科学院黑龙江哈尔滨150081

为获得优质的黑木耳糖蛋白,明确其单糖及氨基酸组成,本研究通过单因素实验和响应面法对超声波辅助碱法提取黑木耳糖蛋白的工艺条件进行优化,并采用AKTAgo纯化仪、DEAE ge Healthcare色谱柱对糖蛋白进行分离纯化,采用高效液相色谱(HPLC)... 详细信息

为获得优质的黑木耳糖蛋白,明确其单糖及氨基酸组成,本研究通过单因素实验和响应面法对超声波辅助碱法提取黑木耳糖蛋白的工艺条件进行优化,并采用AKTAgo纯化仪、DEAE ge Healthcare色谱柱对糖蛋白进行分离纯化,采用高效液相色谱(HPLC)法分析其单糖和氨基酸组成。黑木耳糖蛋白的最佳提取工艺条件为:超声时间90 min、料液比1:90 g/L、超声功率200 W、pH8.4、提取温度45℃,此条件下糖蛋白提取的综合评分为55.93分。经分离纯化得到糖蛋白的纯度为74.1%、蛋白回收率为37.5%,该糖蛋白中的单糖组成以葡萄糖(9.95 mg/g)、甘露糖(5.24 mg/g)为主,氨基酸以L-天冬氨酸/L-天冬酰胺(1.16 mg/g)、L-苯丙氨酸(1.14 mg/g)、L-谷氨酸/L-谷氨酰胺(1.10 mg/g)为主。因此,超声波碱法可有效提高黑木耳糖蛋白提取效率,且黑木耳糖蛋白中含有酸性杂多糖、必需氨基酸齐全,为黑木耳糖蛋白的深入研究及应用开发提供了科学参考。

关键词：黑木耳糖蛋白提取纯化单糖氨基酸

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