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Domesticated cynomolgus monkey embryonic stem cells allow the generation of neonatal interspecies chimeric pigs

Protein & Cell 2020年第2期11卷 97-107页

作者： Rui Fu Dawei Yu Jilong Ren Chongyang Li Jing Wang Guihai Feng Xuepeng Wang Haifeng Wan Tianda Li Libin Wang ying Zhang Tang Hai Wei Li Qi zhou State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China Institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China

Blastocyst complementation by pluripotent stem cell(PSC)injection is believed to be the most promising method to generate xenogeneic ***,ethical issues prevent the study of human chimeras in the late embryonic stage of *** embryonic stem cells(ESCs),which have similar pluripotency to human ESCs,are a good model for studying interspecies chimerism and organ ***,whether primate ESCs can be used in xenogenous grafts remains *** this study,we evaluated the chimeric ability of cynomolgus monkey(Macaca fascicularis)ESCs(cmESCs)in pigs,which are excellent hosts because of their many similarities to *** report an optimized culture medium that enhanced the anti-apoptotic ability of cmESCs and improved the development of chimeric embryos,in which domesticated cmESCs(D-ESCs)injected into pig blastocysts differentiated into cells of all three germ *** addition,we obtained two neonatal interspecies chimeras,in which we observed tissue-specific D-ESC *** together,the results demonstrate the capability of D-ESCs to integrate and differentiate into functional cells in a porcine model,with a chimeric ratio of 0.001-0.0001 in different neonate *** believe this work will facilitate future developments in xenogeneic organogenesis,bringing us one step closer to producing tissue-specific functional cells and organs in a large animal model through interspecies blastocyst complementation.

关键词： embryonic stem cells,blastocyst complementation cynomolgus monkey pig interspecies chimera organ reconstruction

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Novel 18β-glycyrrhetinic acid amide derivatives show dual-acting capabilities for controlling plant bacterial diseases through ROS-mediated antibacterial efficiency and activating plant defense responses

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Journal of Integrative Agriculture 2023年第9期22卷 2759-2771页

作者： SONG ying-lian LIU Hong-wu YANG Yi-hong HE Jing-jing YANG Bin-xin YANG Lin-li zhou Xiang LIU Li-wei WANG Pei-yi YANG Song National Key Laboratory of Green Pesticide/Key Laboratory of Green Pesticide and Agricultural Bioengineering Ministry of Education/Center for R&D of Fine ChemicalsGuizhou UniversityGuiyang 550025P.R.China

Natural products have long been a crucial source of,or provided inspiration for new agrochemical *** occurring 18β-glycyrrhetinic acid shows broad-spectrum bioactivities and is a potential skeleton for novel drug *** extend the utility of 18β-glycyrrhetinic acid for agricultural uses,a series of novel 18β-glycyrrhetinic acid amide derivatives were prepared and evaluated for their antibacterial ***,compound 5k showed good antibacterial activity in vitro against Xanthomonas oryzae ***(Xoo,EC50=3.64 mg L–1),and excellent protective activity(54.68%)against Xoo in *** 5k induced excessive production and accumulation of reactive oxygen species in the tested pathogens,resulting in damaging the bacterial cell *** interestingly,compound 5k could increase the activities of plant defense enzymes including catalase,superoxide dismutase,peroxidase,and phenylalanine ammonia *** together,these enjoyable results suggested that designed compounds derived from 18β-glycyrrhetinic acid showed potential for controlling intractable plant bacterial diseases by disturbing the balance of the phytopathogen’s redox system and activating the plant defense system.

关键词： 18β-glycyrrhetinic acid antibacterial activities defense enzyme activity reactive oxygen species

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Study on blood pressure rhythm in hypertensive patients with Yin deficiency syndrome and a random forest model for predicting hypertension with Yin deficiency syndrome

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Journal of Traditional Chinese Medicine 2024年第3期44卷 564-571页

作者： zhou ying LI Ping LUAN Jianwei SHEN Rui WU yinglan XU Qiwen WANG Xinyue ZHU Yao XU Xiangru LIU Zitian JIANG Yuning ZHONG Yong HE Yun JIANG Weimin Department of Cardiology the Affiliated Hospital of Nanjing University of Chinese MedicineJiangsu Province Hospital of Traditional Chinese MedicineNanjing 210029China Department of Health Management Centre Jinling HospitalAffiliated Hospital of Medical SchoolNanjing UniversityNanjing 210002China Emergency Department Longhua HospitalShanghai University of Traditional Chinese MedicineShanghai 200032China Department of General Internal Medicine the Affiliated Hospital of Nanjing University of Chinese MedicineJiangsu Province Hospital of Traditional Chinese MedicineNanjing 210029China

OBJECTIVE: To investigate blood pressure rhythm(BPR)in Yin deficiency syndrome of hypertension(YDSH)patients and develop a random forest model for predicting ***: Our study was consistent with technical processes and specification for developing guidelines of Evidence-based Chinese medicine clinical practice(T/CACM 1032-2017). We enrolled 234 patients who had been diagnosed with primary hypertension without antihypertensive medications prior to the enrollment. All participants were divided into Yin deficiency group(YX, n = 74) and non-Yin deficiency group(NYX, n = 160).Participants were professionally grouped by three experienced chief Traditional Chinese Medicine(TCM)physicians according to four examinations(i.e.,inspection, listening and smelling, inquiry and palpation).We collected data on 24 h ambulatory blood pressure monitoring(ABPM) and YDSH rating scale. We divided 24 h of a day into 12 two-hour periods [Chen-Shi(7:00-9:00), Si-Shi(9:00-11:00), Wu-Shi(11:00-13:00), Wei-Shi(13:00-15:00), Shen-Shi(15:00-17:00), You-Shi(17:00-19:00), Xu-Shi(19:00-21:00), Hai-Shi(21:00-23:00), ZiShi(23:00-1:00), Chou-Shi(1:00-3:00), Yin-Shi(3:00-5:00), Mao-Shi(5:00-7:00)] according to the theory of “midnight-midday ebb flow”. We used random forest to build the diagnostic model of YDSH, with whether it was Yin deficiency syndrome as the outcome. RESULTS: Compared with NYX group, YX group had more female participants with older age, lower waist circumference, body mass index(BMI), diastolic blood pressure(DBP), and smoking and drinking rate(all P < 0.05). The YDSH rating scores of YX group [28.5(21.0-36.0)] were significantly higher than NYX group [13.0(8.0-22.0)](P < 0.001), and the typical symptoms of YX group included vexing heat in the chest, palms and soles, dizziness, dry eyes, string-like and fine pulse, soreness and weakness of lumbus and knees, palpitations, reddened cheeks, and tinnitus(all P < 0.05). The ratio of non-dipper hypertension in YX group was higher t

关键词： hypertension Yin deficiency syndrome random forest blood pressure rhythm blood pressure variability

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Improved anti-tumor efficacy and pharmacokinetics of bufalin via PEGylated liposomes

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中国药理学与毒理学杂志 2017年第10期31卷 978-979页

作者： Jia-ni YUAN Xuan-xuan zhou Wei CAO Lin-lin BI Yi-fang ZHANG Qian YANG Si-wang WANG Department of Natural Medicine & Institute of Materia Medica School of Pharmacy The Fourth ilitary Medical University Xi ' an China Shaanxi Pharmaceutical Development Center Xi 'an China

OBJECTIVE To determine the characterization,anti-tumor efficacy and pharmacokinetics of bufalin-loaded PEGylated liposomes compared with bufalin *** Bufalin-loaded PEGylated liposomes and bufalin-loaded liposomes were prepared reproducibly with homogeneous particle size by the combination of thin film evaporation method and high pressure homogenization *** particle size and zeta potential of the liposomes were determined by dynamic light scattering *** direct imaging of morphology of liposomes was charactered by transmission electron *** content of bufalin in liposomes was analysed by HPLC *** entrapment efficiency and the particle size was applied to assess the stability profile,after storage at 4℃ on day 0,7,15,30 and *** in-vitro release behaviours of bufalin from liposomes were conducted using dialysis bag technique at 37℃.In-vitro cytotoxicity studies were carried out using MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide]assay on several kinds of tumor cel lines including SW620,PC-3,MDA-MB-231,A549,U251,U87 and ***-vivo pharmacokinetic study of bufalin liposomes was evaluated by HPLC *** Their mean particle sizes were 127.6 nm and 155.0 nm,mean zeta potentials were 2.24 m V and-18.5 m V,entrapment efficiencies were 76.31%and 78.40%,***-vitro release profile revealed that the release of bufalin in bufalin-loaded PEGylated liposomes was slower than that of bufalin-loaded *** cytotoxicity of blank liposomes has been found within acceptable range,whereas bufalin-loaded PEGylated liposomes showed enhanced cytotoxicity to U251 cells compared with bufalin ***-vivo pharmacokinetics indicated that bufalinloaded PEGylated liposomes could extend eliminate half-life time of bufalin in plasma in *** The results suggested that bufalin-loaded PEGylated liposomes improved the solubility and increased the drug concentration in plasma.

关键词： bufalin PEGylated liposome high pressure homogenization pharmacokinetics

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基于Logistic模型的甘肃省区域物流对区域经济发展作用实证研究

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物流技术 2014年第3期33卷 260-262,281页

作者：方颖郑州旅游职业学院河南郑州450009

主要针对甘肃省区域物流和区域经济发展问题展开研究,从定量分析的角度构建了Logistic模型,深入地分析了区域物流对区域经济发展的影响,并应用计量经济学方法对模型进行了弹性分析和预测分析。

关键词：区域物流区域经济 Logistic模型计量经济学方法甘肃省

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Prostate volume does not provide additional predictive value to prostate health index for prostate cancer or clinically significant prostate cancer:results from a multicenter study in China

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Asian Journal of Andrology 2020年第5期22卷 539-543页

作者： Da Huang Yi-Shuo Wu Ding-Wei Ye Jun Qi fang Liu Brian T Helfand Siqun L Zheng Qiang Ding Dan-Feng Xu Rong Na Jian-Feng Xu ying-Hao Sun Department of Urology Ruijin HospitalShanghai Jiao Tong University School of MedicineShanghai 200025China Department of Urology Huashan HospitalFudan UniversityShanghai 200040China Fudan Institute of Urology Huashan HospitalFudan UniversityShanghai 200040China Department of Urology Shanghai Cancer CenterFudan UniversityShanghai 200032China Department of Urology Xinhua HospitalShanghai Jiao Tong University School of MedicineShanghai 200092China Division of Urology NorthShore University Health SystemEvanstonIL 60201USA Program for Personalized Cancer Care NorthShore University Health SystemEvanstonIL 60201US A Departm ent of Urology Shanghai Changhai HospitalThe Second Military Medical UniversityShanghai 2CXD433China.

To evaluate whether prostate volume(PV)would provide additional predictive utility to the prostate health index(phi)for predicting prostate cancer(PCa)or clinically significant prostate cancer,we designed a prospective,observational multicenter study in two prostate biopsy *** 1 included 595 patients from three medical centers from 2012 to 2013,and Cohort 2 included 1025 patients from four medical centers from 2013 to *** under the receiver operating characteristic curves(AUC)and logistic regression models were used to evaluate the predictive performance of PV-based derivatives and *** regression analysis showed that both total prostate-specific antigen(tPSA)and free PSA(fPSA)were significantly correlated with PV(all P0.05).In conclusion,PV-based derivatives(both PHIV and PHID)and models incorporating PV did not improve the predictive abilities of phi for either PCa or clinically significant PCa.

关键词： China prostate cancer prostate health index prostate volume

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社区教育课程设计与实施模式研究

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成人教育 2015年第7期35卷 8-11页

作者：房颖广东省东莞市寮步镇成人文化技术学校广东东莞523400

社区教育课程是社区教育开展的有效媒介,也是社区教育发展的核心内容。各级社区学校要依据社区教育的特点和社区群众的需求设计出内容丰富、实用性强、科学易懂的社区教育课程,以满足社区居民丰富多样的学习需求。同时要积极探索社区教... 详细信息

社区教育课程是社区教育开展的有效媒介,也是社区教育发展的核心内容。各级社区学校要依据社区教育的特点和社区群众的需求设计出内容丰富、实用性强、科学易懂的社区教育课程,以满足社区居民丰富多样的学习需求。同时要积极探索社区教育课程的实施策略,将课程落到实处,真正实现教育活动的目的。

关键词：社区教育课程设计实施模式

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Consensus on diagnostic criteria for Yin deficiency syndrome in hypertension:a modified Delphi study

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Journal of Traditional Chinese Medicine 2023年第1期43卷 181-187页

作者： HE Yun HE Jing XU Xiangru LI Haixiao SHI Rongwei LIANG Junya zhou ying ZHU Yao CHEN Xiaohu TANG Shuhua XIAO Min ZHAN Libin PEI yinghao JIANG Weimin Department of General Internal Medicine Jiangsu Province Hospital of Chinese Medicine Affiliated to Hospital of Nanjing University of Traditional Chinese MedicineNanjing 210029China Department of Hepatology Jiangsu Province Hospital of Chinese Medicine Affiliated to Hospital of Nanjing University of Traditional Chinese MedicineNanjing 210029China Department of Emergency Longhua Hospital affiliated to Shanghai University of Traditional Chinese MedicineShanghai 200032China Department of Institute of Hypertension Jiangsu Province Hospital of Chinese Medicine Affiliated to Hospital of Nanjing University of Traditional Chinese MedicineNanjing 210029China Department of Cardiology Jiangsu Province Hospital of Chinese Medicine Affiliated to Hospital of Nanjing University of Traditional Chinese MedicineNanjing 210029China Department of Cardiology Dongzhimen Hospital Affiliated to Beijing University of Chinese MedicineBeijing 100700China School of Traditional Chinese Medicine&School of Integrated Chinese and Western Medicine Nanjing University of Chinese MedicineNanjing 210023China Department of Intensive Care Unit Jiangsu Province Hospital of Chinese Medicine Affiliated to Hospital of Nanjing University of Traditional Chinese MedicineNanjing 210029China

OBJECTIVE:To reach consensus on the diagnostic criteria for Yin deficiency syndrome in hypertension(YDSH)patients by a modified Delphi ***:Our study was consistent with T/CACM *** methodology of RAND/UCLA appropriateness was used to develop consensus guidance statements.A nationwide panel of experienced clinical experts from 19 provinces was *** experts were all prominent in Traditional Chinese Medicine(TCM)of cardiovascular *** consensus process consisted of two rounds of questionnaires and a final round of consultation to analyze the weight score of each ***,the data extraction process is carried out independently by third-party researchers(LIANG Junya,SUN Yang,and DU Xiaona).When there is disagreement in all three rounds,the expert panel group(odd number)are invited to vote,and the one with more votes *** the questionnaires,participants were asked to rate the appropriateness of each syndrome item using a nine-point Likert *** consensus was defined as a panel median rating 1-3 or 7-9 without *** then the diagnostic criteria of YDSH were formed according to the weight score in the final ***:Twenty-eight experts(84.8%)participated in the first round,and thirty-one(93.9%)finished the second *** two rounds,the consensus of YDSH was reached on 11 items(25.6%),including symptoms,signs,and pulse ***-one experts(63.6%)completed the final round in which they used a grading system for each *** tongue with scanty fur had the highest weighting(22.8%),followed by heat in the palms and soles(20.1%).CONCLUSIONS:The consensus-based diagnostic criteria for YDSH,formed by a modified Delphi method,can be widely incorporated in TCM.A further clinical study will be conducted to analyze the diagnosis value and cut-off score of our YDSH criteria.

关键词： Yin deficiency syndrome hypertension consensus Delphi technique weight score

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Thickness-dependent patterning of MoS2 sheets with well-oriented triangular pits by heating in air

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Nano Research 2013年第10期6卷 703-711页

作者： Haiqing zhou fang Yu Yuanyue Liu Xiaolong Zou Chunxiao Cong Caiyu Qiu Ting Yu Zheng Yan Xiaonan Shen Lianfeng Sun Boris I. Yakobson James M. Tour Division of Physics and Applied Physics School of Physical and Mathematical Sciences Nanyang Technological University 637371Singapore Department of Chemistry Rice University 6100 Main Street Houston Texas 77005 USA The Smalley Institute for Nanoscale Science and Technology Rice University 6100 Main Street Houston Texas 77005 USA Department of Mechanical Engineering and Materials Science Rice University 6100 Main Street Houston Texas 77005 USA National Center for Nanoscience and Technology Beijing 100190 China

Patterning ultrathin MoS2 layers with regular edges or controllable shapes is appealing since the properties of MoS2 sheets are sensitive to the edge structures. In this work, we have introduced a simple, effective and well-controlled technique to etch layered MoS2 sheets with well-oriented equilateral triangular pits by simply heating the samples in air. The anisotropic oxidative etching is greatly affected by the surrounding temperature and the number of MoS2 layers, whereby the pit sizes increase with the increase of surrounding temperature and the number of MoS2 layers. First-principles computations have been performed to explain the formation mechanism of the triangular pits. This technique offers an alternative avenue to engineering the structure of MoS2 sheets.

关键词： layered MoS2 oxidative etching thickness-dependent triangular pits

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多策略融合的SVM决策树构建

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兰州理工大学学报 2012年第3期38卷 98-101页

作者：方莹商丘师范学院计算机与信息技术学院河南商丘476000 北京理工大学计算机学院北京100081

为了在提高文本分类效率和提升分类速度间进行平衡,综合考虑SVM决策树的深度、均衡度、构造方式、类内样本数、类间相似度等对分类结果的影响,提出针对海量文本多分类问题的SVM决策树构建算法.在大规模语料库上的文本分类实验表明,该算... 详细信息

为了在提高文本分类效率和提升分类速度间进行平衡,综合考虑SVM决策树的深度、均衡度、构造方式、类内样本数、类间相似度等对分类结果的影响,提出针对海量文本多分类问题的SVM决策树构建算法.在大规模语料库上的文本分类实验表明,该算法可在一定程度上提升分类效果,同时可以大幅减少训练和测试时间,方法可行且适应性强.

关键词：文本分类支持向量机决策树多类分类器

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