检索结果-南通市图书馆

MODELLING AND COMPUTATION OF UNSTEADY TURBULENT CAVITATION FLOWS

在线全文

学校读者我要写书评

暂无评论

Journal of Hydrodynamics 2006年第5期18卷 559-566页

作者： chen ying lu Chuan-jing WU Lei Department of Engineering Mechanics Shanghai Jiaotong University Shanghai 200240 China

Unsteady turbulent cavitation flows in a Venturi-type section and around a NACA0012 hydrofoil were simulated by two-dimensional computations of viscous compressible turbulent flow model. The Venturi-type section flow proved numerical precision and reliability of the physical model and the code, and further the cavitation around NACA0012 foil was investigated. These flows were calculated with a code of SIMPLE-type finite volume scheme, associated with a barotropic vapor/liquid state law which strongly links density and pressure variation. To simulate turbulent flows, modified RNG k-ε model was used. Numerical results obtained in the Venturi-type flow simulated periodic shedding of sheet cavity and was compared with experiment data, and the results of the NACA0012 foil show quasi-periodic vortex cavitation phenomenon. Results obtained concerning cavity shape and unsteady behavior, void ratio, and velocity field were found in good agreement with experiment ones.

关键词： turbulent cavitation Venturi NACA0012

World Wide Web The Fantastic Resource for Target Culture Teaching in ESL Classroom

在线全文

学校读者我要写书评

暂无评论

Sino-US English Teaching 2004年第10期1卷 25-29页

作者： Hong chen ying lu Xi＇an Institute of Post ＆ Telecommunications

In this paper, a WWW hot list which might be helpful in target culture teaching is provided and a sample lesson is given to illustrate the uses of WWW as great resources in culture teaching in ESL classroom.

关键词： World Wide Web Target Culture Teaching ESL

Epigenetic modification regulates both expression of tumor-associated genes and cell cycle progressing in human colon cancer cell lines: Colo-320 and SW1116

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cell Research 2004年第3期14卷 217-226页

作者： Jing Yuan FANG, ying Xuan chen, Juan lu, Rong lu, Li YANG, Hong Yin ZHU, Wei Qi GU, lun Gen lu Shanghai Institute of Digestive Disease, Renji Hospital Shanghai Second Medical University, Shanghai 200001, China. Shanghai Institute of Digestive Disease Renji Hospital Shanghai Second Medical University Shanghai China

The aim of this study is to assess the effects of DNA methylation and historic acetylation, alone or in combination, on the expression of several tumor-associated genes and cell cycle progression in two established human colon cancer cell lines: Colo-320 and SW1116. Treatments with 5-aza-2’-deoxycytidine (5-aza-dC) and trichostatin A, alone or in combination, were applied respectively. The methylation status of the CDKN2A promoter was determined by methyla-tion-specific PCR, and the acetylated status of the histones associated with the p21WAF1 and CDKN2A genes was examined by chromatin immunoprecipitation. The expression of the CDKN2A, p21WAF1, p53, p73, APC, c-myc, c-Ki-ras and survivin genes was detected by real-time RT-PCR and RT-PCR. The cell cycle profile was established by flow cytometry. We found that along with the demethylation of the CDKN2A gene promoter in both cell lines induced by 5-aza-dC alone or in combination with TSA, the expression of both CDKN2A and APC genes increased. The treatment

关键词： human colon cancer cell lines tumor-associated genes DNA methylation histone acetylation cell cycle.

The protoplast two-way fusions and fusant characteristics of Antrodia cinnamomea and Cordyceps militaris

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Food Science and Human Wellness 2022年第5期11卷 1240-1251页

作者： Wei-Kuang Lai Yang-Chang Wu Tai-Sheng Yeh Chun-Ren Hsieh Yi-Hong Tsai Chien-Kei Wei Chi-ying Li ying-chen lu Fang-Rong Chang Graduate Institute of Natural Products Kaohsiung Medical UniversityKaohsiung 807TaiwanChina Graduate Institute of Integrated Medicine China Medical UniversityTaichung 404TaiwanChina Chinese Medicine Research and Development Center China Medical University HospitalTaichung 404TaiwanChina Department of Food Science and Nutrition Meiho UniversityPingtung 912TaiwanChina Department of Food Science National Chiayi UniversityChiayi City 600TaiwanChina Drug Development and Value Creation Research Center Kaohsiung Medical UniversityKaohsiung 807TaiwanChina Department of Medical Research Kaohsiung Medical University HospitalKaohsiung Medical UniversityKaohsiung 807TaiwanChina Department of Marine Biotechnology and Resources National Sun Yat-sen UniversityKaohsiung 804TaiwanChina

This study generated two fused protoplasts of Antrodia cinnamomea and Cordyceps militaris in two *** protoplasts of *** were inactivated by heat to inactivate biochemical processes and enzymatic activities in the cytoplasm,and the protoplasts of *** were inactivated by UV radiation to invalidate their genome function,then they were fused under optimal conditions to get a fusion rate as(7.42±0.8)×10^(-6) fusants/mL;the new fusants were abbreviated as *** the other hand,when *** and *** were treated with heat and UV oppositely using similar experiments,the fusion rate was(9.70±0.68)×10^(-5) fusants/mL,and the new fusants were abbreviated as *** selected each of two best-growing fused colonies Ac-Cm-1,Ac-Cm-2,Cm-Ac-1,and Cm-Ac-2,together with parental *** and ***,and studied their morphology,growth antagonism tests,and genetic relationships by 18 S rRNA *** comparison with the initial cultures of 4 fusants,the yields of adenosine,biomass,cordycepic acid,cordycepin,total polysaccharide,and total triterpenoids were increased up 1.305-50.1563 times in the optimal medium *** gene stability tests,those of the four fusants and their outputs were stabilized within 10 generations.

关键词： Medicinal fungi Protoplast fusion Antrodia cinnamomea Cordyceps militaris Secondary metabolite

L-type calcium channel blockers enhance 5-HTP-induced antinociception in mice

在线全文

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2004年第5期25卷 102-108页

作者： Jian-hui LIANG1, Jun-xu LI, Xu-hua WANG, Bi chen, ying lu, Pan ZHANG, Rong HAN, Xiang-feng YEDepartment of Neuropharmacology, National Institute on Drug Dependence, Peking University, Beijing 100083, China 北京大学中国药物依赖性研究所北京 100083

AIM: To investigate the involvement of L-type Ca2+ channels in antinociceptive action induced by the 5-HT precursor,5-hydroxytryptophan (5-HTP). METHODS: Female Kunming mice were treated with either 5-HTP (20-80 mg/kg,ip) alone, or the combination of 5-HTP and fluoxetine (2-8 mg/kg, ip), pargyline (15-60 mg/kg, ip), nimodipine (2.5-10 mg/kg, ip), nifedipine (2.5-10 mg/kg, ip), verapamil (2.5-10 mg/kg, ip), CaCl2 (5-20 mmol/L, icv), or EGTA(0.5-3 mmol/L, icv) prior to the hot-plate test (55 C, hind-paw licking latency). In addition, locomotor activity inmice treated with 5-HTP alone was measured using an ambulometer with five activity boxes. RESULTS: Ipinjection of 5-HTP alone had no influence on the spontaneous locomotor activity, whereas dose-dependently in-creased the latency to licking hind-paw in the hot-plate test in mice. The inhibitory effects of 5-HTP on nociceptiveresponse were significantly enhanced by fluoxetine in the mouse hot-plate test. At a sub-effective dose, pargylinecould cause a leftward shift in the dose-response curve of 5-HTP-induced antinociception. Co-administration with5-HTP and nimodipine, nifedipine, or verapamil obviously potentiated the antinociceptive effects elicited by ***, 5-HTP-induced antinociception was antagonized by CaCl2 and enhanced by EGTA injected icv in themouse hot-plate test. CONCluSION: These findings suggest that systemic administration of 5-HTP may yield theantinociceptive effects, which are related to Ca2+ influx from extracellular fluid through L-type Ca2+ channels.

关键词： serotonin 5-hydroxytryptophan fluoxetine pargyline calcium channel blockers pain measure-ment

APPLICATION OF 2D-NMR TECHNIQUES IN THE STRUCTURE DETERMINATION OF HYPODEMATINE

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Chinese Chemical Letters 1990年第3期1卷 221-222页

作者： Ya Min chen ying Hua lu Yan Jun chen Cun Sheng MA (Jining Medical College,Shandong,272113) De Quan YU (Institute of Materia Medica,Chinese Academy of Medical Sciences,Beijing,100050)

An alkaloid with a novel structure,named Hypodematine,was isolated from Hypodematium sinense Iwatsuki(belonging to Thelypterdaceae).Its structure was elucidated by means of the ~1H-~1H COSY' ~1H-^(13)C COSY and long-range ~1H-^(13)C COSY spectroscopy to have the skeleton of benzo-aza-cyclooctatetraene with a phenyl *** basic structure has not been found in the na- tural product before.

关键词： NMR APPLICATION OF 2D-NMR TECHNIQUES IN THE STRUCTURE DETERMINATION OF HYPODEMATINE

Low radiation and low contrast dose coronary CT angiography at 64-row CT:usefulness of BMI-adapted protocol and the iterative reconstruction algorithm

在线全文

学校读者我要写书评

暂无评论

Low radiation and low contrast dose coronary CT angiography ...

STUDIES ON SUCCINIC DEHYDROGENASE Ⅱ.FURTHER OBSERVATIONS ON THE PROPERTIES OF THE ENZYME AND ITS PROSTHETIC GROUP

第四届全国医学影像中西医结合青年医师论坛暨第四届《中国中西医结合影像学杂志》编委会会议

作者： chen-ying lu Jian-song Ji Hai-lin Wang Xiang-hua Hu Zu-fei Wang Chun-miao chen Radiology Department Lishui Hospital of Zhejiang University

Objective—To evaluate the effect on image quality of a low radiation and low contrast dose for coronary computed tomography angiography(CCTA)using a BMI-adapted protocol and the iterative reconstruction algorithm with prospective ECG triggering Materials and Methods—196 consecutive patients with clinical suspicion of coronary disease were prospectively *** weight and height were measured before *** iterative reconstruction technique were *** were divided into four groups using kV/***=80/200,100/150,120/150 and 140/150 when patient’s BMI was ≤18.5(n=21),18.5-24.0(n=76),24.0-28.0(n=64) and>28.0(n=35),*** contrast dose and flow rate was divided into 50ml/4.0 mL/s,60ml/4.5mL/s,70ml/5.0 mL/s,70ml/5.0 mL/s *** quality was independently assessed per-segment by two experienced *** noise and attenuation were ***-to-noise ratio(SNR) and contrast-to-noise ratio(CNR) were *** effective radiation dose was calculated using CT dose volume index and the dose-length ***—BMI ranged from 16.3 to 33.6 cm,the contrast dose was 50ml-70 ml and the mean effective radiation dose was 0.9-3.4 *** image quality scores indicated no significant difference among the four groups(P=0.376).Image noise,vessel attenuation and SNR were higher in 80 kV and 100 kV groups than that of 120 kV and 140 kV groups(P<0.05),while CNR in RCA and LM were not significantly different among the four groups(P = 0.079 and P = 0.264),supporting the validity of the BMI-adapted scanning protocol and contrast ***—BMI-adapted protocol and the iterative reconstruction algorithm can be used for individualized radiation dose and contrast control,resulting in similar CNR and maintaining diagnostic image quality.

关键词： BMI computed tomography coronary angiography radiation dose contrast dose image quality

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Science in China,Ser.A 1958年第1期 65-74页

作者：汪静英邹承鲁王应睐 Institute of Physiology and Biochemistry Academia Sinica Shanghai

In our previous reports,we have described the purification and certainproperties of soluble succinic dehydrogenase of pig heart as well as somepreliminary observations on the prosthetic group of the purified *** enzyme is a metalloflavoprotein containing non-hematin iron,the molarratio of iron and iso-alloxazine being 4:*** calculated molecular weightof succinic dehydrogenase is approximately 140,000—160,000,assuming thateach mole of the enzyme contains 1 mole of *** present communicationrecords our further observations on the properties of the enzyme and itsprosthetic group.

关键词： FURTHER OBSERVATIONS ON THE PROPERTIES OF THE ENZYME AND ITS PROSTHETIC GROUP STUDIES ON SUCCINIC DEHYDROGENASE FAD

亚麻种子中胰蛋白酶抑制剂的分离纯化及性质研究

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

食品工业科技 2016年第22期37卷 234-239页

作者：陈颖璐石亚伟山西大学生物技术研究所教育部化学生物学与分子工程重点实验室山西太原030006

将亚麻种子去壳粉碎,经丙酮脱脂和Tris-HCl缓冲液提取后,Q-Sepharose^(TM) Fast flow离子交换层析一步纯化,获得电泳纯的亚麻胰蛋白酶抑制剂(luTI),纯化倍数可达9.62,活力回收率为6.25%,比活力为55.35 U/mg,SDSPAGE电泳显示luTI分子量... 详细信息

将亚麻种子去壳粉碎,经丙酮脱脂和Tris-HCl缓冲液提取后,Q-Sepharose^(TM) Fast flow离子交换层析一步纯化,获得电泳纯的亚麻胰蛋白酶抑制剂(luTI),纯化倍数可达9.62,活力回收率为6.25%,比活力为55.35 U/mg,SDSPAGE电泳显示luTI分子量大小约为8 ku。质谱鉴定属于Potato型胰蛋白酶抑制剂,其抑制活性在p H2.0~6.0以及70℃以下有较好的稳定性,最适p H为6.0,最适温度为40℃,属于一种非竞争性抑制剂,Ki值为9.18×10^(-4)mol/L。DTNB法检测luTI含有一对二硫键,二硫键存在有助于提高luTI的稳定性和活性。

关键词：亚麻种子胰蛋白酶抑制剂分离纯化酶活测定