The protoplast two-way fusions and fusant characteristics of Antrodia cinnamomea and Cordyceps militaris

作     者：Wei-Kuang Lai Yang-Chang Wu Tai-Sheng Yeh Chun-Ren Hsieh Yi-Hong Tsai Chien-Kei Wei Chi-Ying Li Ying-Chen Lu Fang-Rong Chang Wei-Kuang Lai;Yang-Chang Wu;Tai-Sheng Yeh;Chun-Ren Hsieh;Yi-Hong Tsai;Chien-Kei Wei;Chi-Ying Li;Ying-Chen Lu;Fang-Rong Chang

作者机构：Graduate Institute of Natural ProductsKaohsiung Medical UniversityKaohsiung 807TaiwanChina Graduate Institute of Integrated MedicineChina Medical UniversityTaichung 404TaiwanChina Chinese Medicine Research and Development CenterChina Medical University HospitalTaichung 404TaiwanChina Department of Food Science and NutritionMeiho UniversityPingtung 912TaiwanChina Department of Food ScienceNational Chiayi UniversityChiayi City 600TaiwanChina Drug Development and Value Creation Research CenterKaohsiung Medical UniversityKaohsiung 807TaiwanChina Department of Medical ResearchKaohsiung Medical University HospitalKaohsiung Medical UniversityKaohsiung 807TaiwanChina Department of Marine Biotechnology and ResourcesNational Sun Yat-sen UniversityKaohsiung 804TaiwanChina 

出 版 物：《Food Science and Human Wellness》 (食品科学与人类健康（英文）)

年 卷 期：2022年第11卷第5期

页      面：1240-1251页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 10[医学] 

基　　金：supported by grants from the Ministry of Science and Technology of Taiwan(Grant number:MOST 106-2320-B-037008-MY2,MOST 108-2320-B-037-022-MY3,108-2811-B-037-511,and 109-2927-I-037-502) funded by the Drug Development and Value Creation Research Center,Kaohsiung Medical University Department of Medical Research,Kaohsiung Medical University Hospital(Grant number:KMU-TC108A03-11) 

主　　题：Medicinal fungi Protoplast fusion Antrodia cinnamomea Cordyceps militaris Secondary metabolite 

摘      要：This study generated two fused protoplasts of Antrodia cinnamomea and Cordyceps militaris in two *** protoplasts of *** were inactivated by heat to inactivate biochemical processes and enzymatic activities in the cytoplasm,and the protoplasts of *** were inactivated by UV radiation to invalidate their genome function,then they were fused under optimal conditions to get a fusion rate as(7.42±0.8)×10^(-6) fusants/mL;the new fusants were abbreviated as *** the other hand,when *** and *** were treated with heat and UV oppositely using similar experiments,the fusion rate was(9.70±0.68)×10^(-5) fusants/mL,and the new fusants were abbreviated as *** selected each of two best-growing fused colonies Ac-Cm-1,Ac-Cm-2,Cm-Ac-1,and Cm-Ac-2,together with parental *** and ***,and studied their morphology,growth antagonism tests,and genetic relationships by 18 S rRNA *** comparison with the initial cultures of 4 fusants,the yields of adenosine,biomass,cordycepic acid,cordycepin,total polysaccharide,and total triterpenoids were increased up 1.305-50.1563 times in the optimal medium *** gene stability tests,those of the four fusants and their outputs were stabilized within 10 generations.