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Generation of male germ cells in vitro from the stem cells

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Asian Journal of Andrology 2023年第1期25卷 13-20页

作者： ying-hong Cui Wei Chen Si Wu Cai-Lin Wan Zuping He The Key Laboratory of Model Animals and Stem Cell Biology in Hunan Province Hunan Normal University School of MedicineThe Manufacture-Based Learning and Research Demonstration Center for Human Reproductive Health New Technology of Hunan Normal UniversityChangsha 410013China

Infertility has become a serious disease since it affects 10%–15%of couples worldwide,and male infertility contributes to about 50%of the ***,a significant decrease occurs in the newborn population by 7.82 million in 2020 compared to 2016 in *** such,it is essential to explore the effective methods of obtaining functional male gametes for restoring male *** cells,including embryonic stem cells(ESCs),induced pluripotent stem cells(iPSCs),spermatogonial stem cells(SSCs),and mesenchymal stem cells(MSCs),possess the abilities of both self-renewal and differentiation into germ ***,much progress has recently been achieved in the generation of male germ cells in vitro from various kinds of stem cells under the specified conditions,e.g.,the coculturing with Sertoli cells,three-dimensional culture system,the addition of growth factors and cytokines,and/or the overexpression of germ cell-related *** this review,we address the current advance in the derivation of male germ cells in vitro from stem cells based on the studies of the peers and us,and we highlight the perspectives and potential application of stem cell-derived male gametes in reproductive medicine.

关键词： application in vitro generation male germ cells stem cells

Ionic liquid-assisted synthesis of silver nanorods by polyol reduction

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Chinese Chemical Letters 2010年第7期21卷 872-875页

作者： hong ying Wang Yan An Gao Ming Wei Zhao Li Qiang Zheng Key Laboratory of Colloid and Interface Chemistry of Ministry of Education in Shandong University Jinan 250100China

Silver nanorods have been successfully synthesized in large scale by the ethylene glycol（EG） reduction in the presence of ionic liquid（IL） 1-butyl-3-methylimidazolium tetrafluoroborate（bmimBF_4） and polyvinyl-pyrrolidone（PVP）.The silver nanorods were characterized by scanning electron microscopy（SEM）,high-resolution transmission electron microscopy（HRTEM）,transmission electron microscopy（TEM）,electron energy disperse spectroscopy（EDS） and UV-vis *** results showed that the uniform silver nanorods have an average diameter of about 100 nm and the aspect ratio from 15 to ***,bmimBF_4 may play a role of capping agent together with PVP in the formation of silver *** the other band,bmimBF_4 may accelerate nucleation and improve the stability of the resulting Ag nanorods due to the low interface tension of IL.

关键词： Silver nanorods Ionic liquid Ethylene glycol Capping agent

An improved gas chromatography for rapid measurement of CO2, CH4 and N2O

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Journal of Environmental Sciences 2006年第1期18卷 162-169页

作者： WANG ying-hong WANG Yue-si SUN Yang XU Zhong-jun LIU Guang-ren Institute of Atmospheric Physics Chinese Academy of Sciences Beijing 100029 China Graduate School of the Chinese Academy of Sciences Beijing 100049 China

Combining improved injector, gas line and valve-driving modules, a GC equipped with FID and ECD, could simultaneously measure CH4, CO2 and N2O in an air sample within 4 min. Test results showed that the system has high sensitivity, resolution and precision; the linear response range of the system meets the requirement of in situ flux measurements. Thus, the system is suitable for monitoring fluxes of main greenhouse gases in terrestrial ecosystem since it is easy to use, efficacious, stable and reliable to collect data.

关键词： GC valve emission of greenhouse gases ecosystem

Fuzzy Neural Model for Flatness Pattern Recognition

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Journal of Iron and Steel Research International 2008年第6期15卷 33-38页

作者： JIA Chun-yu SHAN Xiu-ying LIU hong-min NIU Zhao-ping School of Mechanical Engineering Yanshan University Qinhuangdao 066004 Hebei China

For the problems occurring in a least square method model, a fuzzy model, and a neural network model for flatness pattern recognition, a fuzzy neural network model for flatness pattern recognition with only three-input and three output signals was proposed with Legendre orthodoxy polynomial as basic pattern, based on fuzzy logic expert experiential knowledge and genetic-BP hybrid optimization algorithm. The model not only had definite physical meanings in its inner nodes, but also had strong self-adaptability, anti interference ability, high recognition precision, and high velocity, thereby meeting the demand of high-precision flatness control for cold strip mill and providing a convenient, practical, and novel method for flatness pattern recognition.

关键词： flatness pattern recognition Legendre orthodoxy polynomial genetic-BP algorithm fuzzy neural network

Influence of Solvent Size on Structural Properties of Bottle-brush Polymers

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Chemical Research in Chinese Universities 2010年第5期26卷 838-841页

作者： LIU ying-tao LIU hong LIU Yu-hua LU Zhong-yuan State Key Laboratory of Theoretical and Computational Chemistry Institute of Theoretical Chemistry Jilin University Changchun 130021 P. R. China

We proposed a modified dissipative particle dynamics simulation model by which one can effectively avoid the bond-crossing problem, and investigated the effect of solvent size on the structural properties of bottle-brush polymers in dilute solution on the basis of this model. It was found that with the increase of solvent size, the radius of the gyration of the bottle-brush polymer decreases considerably in the athermal solvent but increases in the selective solvent favoring the backbone, respectively.

关键词： Dissipative particle dynamics Bottle-brush polymer Solvent size Bond-crossing

Inhibitory effects of miRNA-200c on chemotherapy-resistance and cell proliferation of gastric cancer SGC7901/DDP cells

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Chinese Journal of Cancer 2010年第12期29卷 1006-1011页

作者： Yong Chen Jing Zuo ying Liu hong Gao Wei Liu Department of Medical Oncology Hebei Medical University Shijiazhuang Hebei 050011 P. R. China

Background and Objective: miRNA-200c can not only inhibit the aggressiveness of cancer cells but also increase the sensitivity of cells to antitumor drugs. However, some mechanisms are still unclear. Recent researches revealed that E-cadherin is more than an inhibitor of metastasis, and it also plays important roles in reversing drug resistance. We had previously found that miRNA-200c could not only induce the expression of E-cadherin but also increase the sensitivity of gastric cancer SGC7901/DDP cells to cisplatin (DDP). This study aimed to explore the effects of miRNA-200c on biological characteristics of SGC7901/DDP cells and the roles of E-cadherin in the regulatory pathway of miRNA-200c. Methods: SGC7901/DDP cells and its parental cell line SGC7901 cells were transfected with miRNA-200c precursor (Pre-200c) and E-cadherin siRNA, respectively. Real-time RT-PCR was used to detect miRNA-200c expression after transfection with Pre-200c in SGC7901/DDP cell line. Drug sensitivities to DDP, 5-fluorouracil (5-FU), paclitaxel, and adriamycin (ADR) after transfection were tested using MTT assay. The proliferation of SGC7901/DDP cells was also detected after transfection. The protein changes of E-cadherin, Bax, and Bcl-2 after transfection were detected by Western blot. Results: The miRNA-200c expression in SGC7901/DDP cells after transfection of Pre-200c was 7.128 ± 0.159 times of that in negative control (P < 0.05). The IC50 of DDP, 5-FU, paclitaxel, and ADR in Pre-200c-transfected group were significantly lower than that in negative control group (P < 0.05). Compared to the control group, cell proliferation was significantly decreased (P < 0.05). The relative protein expressions of E-cadherin and Bax in Pre-200c-transfected group were significantly higher than those in negative control group (P < 0.05), whereas Bcl-2 was significantly lower than that in control (P < 0.05). Additionally, E-cadherin protein expression was significantly inhibited after transfected with E

关键词： miRNA 增殖细胞胃癌细胞 C型 siRNA 细胞转染钙粘蛋白化疗

Possible mechanism for hepatitis B virus X gene to induce apoptosis of hepatocytes

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World Journal of Gastroenterology 2005年第28期11卷 4351-4356页

作者： Sheng-Jun Zhang hong-ying Chen Zhi-Xin Chen Xiao-Zhong Wang Department of Gastroenterology Union Hospital of Fujian Medical University Fuzhou 350001 Fujian Province China

AIM： To investigate the possible mechanism for HBV X gene to induce apoptosis of hepatocyte HL-7702 cells.METHODS： HBV X gene eukaryon expression vector pcDNA3-X was established and transfected into HL-7702 cells by lipid-mediated transfection, including transient and stable transfection. Positive clones were screened by incubating in the selective medium with 600 μg/mL G418 and named HL-7702/HBV-encoded X protein （HBx） cells. The expressions of Fas/FasL, Bax/Bcl-2, and c-myc mRNA were measured by semi-quantitative RT-PCR in HL-7702/HBx and control group, respectively.RESULTS： RT-PCR analysis confirmed that HBV X gene was transfected into HL-7702 cells successfully. By semiquantitative RT-PCR analysis, Bax and c-myc mRNA levels in HL-7702/HBx cells of transient transfection were significantly higher than those in control, FasL and c-myc mRNA levels in HL-7702/HBx cells of stable transfection were significantly higher than those in control, whereas the Bcl-2 mRNA levels in HL-7702/HBx cells of transient and stable transfection were significantly lower than thosein control.CONCLUSION： HBV X gene may promote the apoptosis of hepatocytes by regulating the expressions of Fas/FasL, Bax/Bcl-2, and c-myc gene in a dose-dependent manner.

关键词： Hepatitis B virus X gene Apoptosis Geneexpression

Successful treatment of granulomatosis with polyangiitis using tocilizumab combined with glucocorticoids:A case report

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World Journal of Clinical Cases 2023年第5期11卷 1144-1151页

作者： Peng-Fang Tang Long-Chuan Xu Wen-Ting hong hong-ying Shi Department of Rheumatology Second Affiliated Hospital of Fujian Medical UniversityQuanzhou 362000Fujian ProvinceChina Department of Pathology Second Affiliated Hospital of Fujian Medical UniversityQuanzhou 362000Fujian ProvinceChina Department of Immunology Second Affiliated Hospital of Fujian Medical UniversityQuanzhou 362000Fujian ProvinceChina

BACKGROUND Tocilizumab is a humanized monoclonal antibody against the interleukin-6(IL-6)receptor that is commonly used to treat large vessel vasculitis and antineutrophil cytoplasmic antibody-related small vessel ***,tocilizumab in combination with glucocorticoids for successfully treating granulomatosis with polyangiitis(GPA)has rarely been *** SUMMARY Here,we report a 40-year-old male patient who suffered GPA for 4 *** was treated with multiple rounds of drugs,including cyclophosphamide,Tripterygium wilfordii,mycophenolate mofetil,and belimumab,with no *** addition,he exhibited persistently high IL-6 *** tocilizumab treatment,his symptoms improved,and his inflammatory marker levels returned to *** Tocilizumab may be effective for treating GPA.

关键词： Tocilizumab Granulomatosis with polyangiitis Antineutrophil cytoplasmic antibody Case report

Inactivation and Regrowth of Antibiotic-resistant Bacteria by PAA Disinfection in the Secondary Effluent of a Municipal Wastewater Treatment Plant

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Biomedical and Environmental Sciences 2013年第10期26卷 865-868页

作者： HUANG Jing Jing XI Jing ying HU hong ying TANG Fang PANG Yu Chen State Key Joint Laboratory of Environmental Simulation and Pollution Control School of EnvironmentTsinghua University China PowerEngineering Consulting Group Corporation Graduate School at Shenzhen Tsinghua University

Inactivation and microbial regrowth of penicillin-, ampicillin-, cefalexin-, tetracycline-, chloramphenicol-, and rifampicin-resistant bacteria were studied to explore risks associated with selection and regrowth of antibiotic-resistant bacteria after PAA disinfection. The results showed that after exposure to 20 mg/L PAA for 10 min, inactivation of ampicillin-resistant bacteria reached 2.3-1og, which was significantly higher than that of total heterotrophic bacteria with a decrease of 2.0-log. In contrast, inactivation of tetracycline- resistant bacteria was significantly less efficient, reaching only 1.1-1og. Chloramphenicol-and tetracycline-resistant bacteria, as well as total heterotrophic bacteria regrew more than 10 fold compared to those in the untreated wastewater sample with 22 h stilling culture after exposure to 2 or 5 mg/L PAA as for 10 min.

关键词： Inactivation microbial ampicillin