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妊娠期糖尿病患者产后随访管理的证据总结

中国护理管理 2024年第4期24卷 540-545页

作者：盖莹李唱耿小婷姜海龙李淑英承德医学院护理学院河北省承德市067000 承德医学院附属医院护理部

目的:总结妊娠期糖尿病患者产后随访管理的证据,为临床实践提供参考。方法:系统检索计算机决策系统、指南网、专业协会网站、循证文献资源库及原始文献数据库中关于妊娠期糖尿病患者产后随访及管理的证据,包括临床决策、指南、系统评价... 详细信息

目的:总结妊娠期糖尿病患者产后随访管理的证据,为临床实践提供参考。方法:系统检索计算机决策系统、指南网、专业协会网站、循证文献资源库及原始文献数据库中关于妊娠期糖尿病患者产后随访及管理的证据,包括临床决策、指南、系统评价、证据总结、专家共识等,检索时限为2010年1月至2023年12月。由2名研究者独立对文献质量进行评价,提取并汇总形成最佳证据。结果:共纳入18篇文献,包括临床决策3篇、指南8篇、专家共识1篇、系统评价5篇、随机对照试验1篇,最终汇总的妊娠期糖尿病患者产后随访管理的证据包括随访的必要性、随访管理的内容、随访的障碍因素及促进随访的方式4个方面共20条证据。结论:本研究总结了关于妊娠期糖尿病患者产后随访管理的证据,为临床医护人员科学、规范地进行妊娠糖尿病患者产后随访提供参考依据。

关键词：妊娠期糖尿病随访产后管理循证护理学

A Ten-year Retrospective Study of Invasive Candidiasis in a Tertiary Hospital in Beijing

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Biomedical and Environmental Sciences 2021年第10期34卷 773-788页

作者： YANG Zhi Hui SONG ying gai LI Ruo Yu Department of Dermatology and Venerology Beijing Key Laboratory of Molecular Diagnosis of DermatosesNational Clinical Research Center for Skin and Immune DiseasesNMPA Key Laboratory for Quality Control and Evaluation of CosmeticsPeking University First HospitalBeijing 100034China

Objective This study aimed to evaluate the epidemiological,clinical and mycological characteristics of invasive candidiasis(IC)in *** A ten-year retrospective study including 183 IC episodes was conducted in a tertiary hospital in Beijing,*** The overall incidence of IC from 2010–2019 was 0.261 episodes per 1,000 ***(71.0%)was the major infective pattern;70.3%of the patients tested positive for Candida *** before IC and the median time to develop an invasive infection after colonization was13.5 days(interquartile range:4.5–37.0 days).Candida albicans(45.8%)was the most prevalent species,followed by Candida parapsilosis(19.5%),Candida glabrata(14.2%)and Candida tropicalis(13.7%).***-albicans IC was more common in patients with severe anemia(P=0.018),long-term hospitalization(P=0.015),hematologic malignancies(P=0.002),continuous administration of broad-spectrum antibiotics(P<0.001)and mechanical ventilation(P=0.012).In vitro resistance testing showed that11.0%of the Candida isolates were resistant/non-wild type(non-WT)to fluconazole,followed by voriconazole(9.6%),micafungin(3.8%),and caspofungin(2.9%).Fluconazole was the most commonly used drug to initiate antifungal therapy both before and after the proven diagnosis(52.6%and 54.6%,respectively).The 30-day and 90-day all-cause mortality rates were 24.5%and 32.7%,*** The incidence of IC has declined in the recent five ***-albicans contributed to more than half of the IC *** can be used as first-line therapy if resistant strains are not ***,multi-center surveillance of the clinical and mycological characteristics of IC is required.

关键词： Invasive candidiasis Epidemiology Risk factors Antifungal resistance Treatment Mortality

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产后腹直肌分离康复管理的证据总结

中国护理管理 2024年第4期24卷 546-550页

作者：耿小婷李淑英盖莹李唱姜海龙郭艳巍承德医学院附属医院护理部河北省承德市067000 承德医学院附属医院产科河北省承德市067000 承德医学院

目的:总结产后腹直肌分离康复管理的证据,以期为临床提供借鉴与指导。方法:计算机检索国内外专业网站、数据库中有关产后腹直肌分离康复管理的相关证据,检索时限为建库至2023年5月。由系统学习过循证方法的研究人员进行文献质量评价,并... 详细信息

目的:总结产后腹直肌分离康复管理的证据,以期为临床提供借鉴与指导。方法:计算机检索国内外专业网站、数据库中有关产后腹直肌分离康复管理的相关证据,检索时限为建库至2023年5月。由系统学习过循证方法的研究人员进行文献质量评价,并对证据进行提取。结果:最终纳入9篇文献,2篇指南、1篇系统评价、1篇专家共识、5篇随机对照试验。通过对纳入文献的分析,最终总结了21条关于产后腹直肌分离康复管理的证据,包括识别影响因素、判断标准、康复方法、健康教育4个类别。结论:本研究通过循证的方法总结的证据将为产后腹直肌分离康复提供科学、详细的管理建议,减少因忽视对腹直肌分离的管理为产妇带来的不良影响,进而提高产妇的生活质量。同时建议临床工作人员提高对产后腹直肌分离康复管理的重视,结合本科室的临床情境与产妇个人意愿进行实践。

关键词：产妇腹直肌分离康复证据总结

Quantitative Analysis of Cytokinins in Plants by High Performance Liquid Chromatography:Electronspray Ionization Ion Trap Mass Spectrometry

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Journal of Integrative Plant Biology 2010年第10期52卷 925-932页

作者： Weiqi Chen ying gai Shichang Liu Renxiao Wang Xiangning Jiang College of Life Sciencesand Biotechnology Bering Forestry University The Tree and Ornamental Plant Bree~ng and BiotechnologyLaboratory of Chinese Forestry Administration National Engineering Laboratory for Tree Breeding Beijing 100083 China Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China

The present paper introduces a highly sensitive and selective method for simultaneous quantification of 12 cytokinins （free form and their conjugates）. The method includes a protocol of extraction with methanol/water/formic acid （15/4/1, v/v/v） to the micro-scale samples, pre-purification with solid phase extraction （SPE） cartridges of the extracts, separation with a high performance liquid chromatography （HPLC） and detection by an electrospray ionization ion trap mass spectrometry （ESl-lon trap-MS） system in a consecutive ion monitoring （CRM） mode at the three stage fragmentation of mass spectrometry （MS3）. The lowest detection level of the cytokinins of the method reaches 0.1-2.0 pg with a very wide range of linear regression from 1-512 pg, at the coefficient factors of 0.98-0.99. The feasibility of this method has been proven in the application of the method to the analysis of the trace-amount contents of cytokinins in the micro-scale samples of various types of plant materials, such as aerial parts of rice and poplar leaves etc. 12 endogenous cytokinins had been identified and quantified in the plant tissues, with an acceptable relatively higher recovery rate from 40% to 70%.

关键词： Methanol Cytokinins Extraction Extracts Methods Mass spectrometry Weight Traps Trapping Ions

Construction and analysis of a plant transformation binary vector pBDGG harboring a bi-directional promoter fusing dual visible reporter genes

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Journal of Genetics and Genomics 2008年第4期35卷 245-249页

作者： Chunxiao Zhang ying gai Wenqi Wang Yanyan Zhu Xuemei Chen Xiangning Jiang College of Bioscience and Bioengineering Hebei University of Science ＆ Technology Shijiazhuang 050018 China College of Life Sciences and Biotechnology Beijing Forestry University The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry Administration Beijing 100083 China Department of Science and Technology Yanshan University Qinhuangdao 066004 China

The constitutive promoter of cauliflower mosaic virus 35S （CaMV 35S） is a polar unidirectional promoter and is widely used in plant genetic engineering. In the present study, the unidirectional CaMV 35S promoter has been modified to a bi-directional promoter by fusing its minimal promoter element to the 5＇ end of CaMV 35S promoter in the opposite orientation. To qualitatively and quantitatively estimate its bi-directional transcriptional function and activity, two visible reporter genes, gusA （13-glucuronidase, GUS） and gfp （green fluorescent protein, GFP）, were fused to the two ends of the promoter in bi-orientations ending with NOS terminator sequences, respectively. Stable expression of gusA and gfp genes in transgenic tobacco （Nicotiana tabacum L.） was visulized by histochemically staining for GUS and fluorescence microscopic observation under UV for GFP in transgenic plants. The expression of two reporter genes showed that the constructed bi-directional promoter did have the bi-directional transcriptional function in both expected orientations. The quantitative estimation of GUS and GFP were determined on a HITACHI F1000 Fluorescence Spectrophotometer with various wavelengths of excitation and emission. The GUS activity varied from g to 250 pmol 4-MU/min/mg protein and the GFP content varied from 0.9 to 1.8 μg/ mg protein in various lines of transgenic tobacco plants. Higher GUS activity generally coupled with lower GFP content, and vice versa.

关键词： bi-directional promoter gusA gene gfp gene Nicotiana tabacum L. expression

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成人牙周病预防及管理的最佳证据总结

中国医药科学 2024年第5期14卷 181-185页

作者：张继敏李曼茹盖莹刘锐承德医学院河北承德067000 承德医学院附属医院口腔科河北承德067000

目的总结成人牙周病预防及管理的最佳证据,为临床实践提供参考。方法系统检索国内外数据库中关于成人牙周病预防及管理的相关证据,检索时限为建库至2023年3月。由2名研究者独立对文献质量进行评价,并提取、汇总相关内容形成最佳证据。... 详细信息

目的总结成人牙周病预防及管理的最佳证据,为临床实践提供参考。方法系统检索国内外数据库中关于成人牙周病预防及管理的相关证据,检索时限为建库至2023年3月。由2名研究者独立对文献质量进行评价,并提取、汇总相关内容形成最佳证据。结果共纳入12篇文献,包括1篇临床决策、5篇指南、4篇专家共识及2篇系统评价,最终获取了18条最佳证据,包括风险评估、口腔卫生指导、健康教育、随访4个方面。结论本研究汇总的证据具备一定的科学性与实用性,建议医护人员在证据转化时应充分结合实际情况、患者偏好,制订相应的预防及管理方案,降低牙周病的发生率、维护患者的牙周健康。

关键词：牙周病口腔健康循证护理学证据总结

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妊娠期糖尿病产妇产后随访清单的构建与应用

中华护理教育 2024年第4期21卷 389-396页

作者：盖莹李唱张继敏耿小婷姜海龙李淑英郭艳巍承德医学院承德市067000 承德医学院附属医院

目的构建妊娠期糖尿病产妇产后随访清单,以规范妊娠期糖尿病产妇产后随访内容及流程。方法以证据为基础构建妊娠期糖尿病产后随访清单初稿。于2023年3月—5月采用德尔菲法对18名专家进行2轮函询,根据专家意见修改,形成试用版妊娠期糖尿... 详细信息

目的构建妊娠期糖尿病产妇产后随访清单,以规范妊娠期糖尿病产妇产后随访内容及流程。方法以证据为基础构建妊娠期糖尿病产后随访清单初稿。于2023年3月—5月采用德尔菲法对18名专家进行2轮函询,根据专家意见修改,形成试用版妊娠期糖尿病产后随访清单;2023年5月,便利选取20名妊娠期糖尿病产妇初步试用该随访清单,再次修订后形成清单终稿。结果2轮专家函询的积极系数均为100%(18/18);专家权威系数为0.914、0.919;Kendall协调系数分别为0.214、0.188(均P<0.001)。最终形成的妊娠期糖尿病产妇产后随访清单包括6个一级条目(产后糖脂代谢管理状况、健康行为管理状况、产后心理状况、子代健康状况、家庭社会支持状况、产后健康指导)、18个二级条目及46个三级条目。清单初步试用阶段,随访时长为3.45~10.18(5.76±1.89)min/次,随访护士及受访产妇对随访清单的作用予以肯定,并增加一份妊娠期糖尿病产后护理健康指导手册。结论本研究构建的产后随访清单内容科学、全面,能为妊娠期糖尿病产妇产后随访提供参考。

关键词：妊娠期糖尿病产后护理随访清单德尔菲技术

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血液透析患者口渴症状管理的最佳证据总结

中国血液净化 2024年第9期23卷 716-720页

作者：姜海龙李唱盖莹耿小婷张池李淑英承德医学院护理学院承德067000 承德医学院附属医院护理部承德067020 承德医学院附属医院手术室承德067020

目的评价总结血液透析患者口渴症状管理的最佳证据,为临床实践提供循证证据。方法系统检索国内外计算机决策支持系统、指南网站、数据库、相关专业协会网站中与血液透析患者口渴症状管理的相关文献,检索时限为建库至2023年11月。采用AG... 详细信息

目的评价总结血液透析患者口渴症状管理的最佳证据,为临床实践提供循证证据。方法系统检索国内外计算机决策支持系统、指南网站、数据库、相关专业协会网站中与血液透析患者口渴症状管理的相关文献,检索时限为建库至2023年11月。采用AGREEⅡ、JBI及CASE评价工具对纳入的文献进行质量评价,对符合要求的文献进行证据总结和归纳。结果最终共纳入12篇文献,包括指南1篇、证据总结1篇、系统评价3篇、随机对照试验7篇。结论本研究对血液透析患者口渴症状管理的最佳证据进行总结,为医护人员在临床实践过程中提供科学依据。

关键词：血液透析口渴证据总结循证护理学

Somatic Embryogenesis and Plant Regeneration from Two Recalcitrant Genotypes of Gossypium hirsutum L.

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Agricultural Sciences in China 2006年第5期5卷 323-329页

作者： WANG Yan-xia WANG Xing-fen MA Zhi-ying ZHANG Gui-yin HAN gai-ying Hebei Agricultural University/Key Laboratory of Crop Germplasm Resources of Hebei Baoding 071001 P.R. China

An improved protocol has been developed for somatic embryogenesis and plant regeneration of recalcitrant cotton cultivars. High callus frequencies and embryogenic tissue were developed in MSB medium supplemented with gradient concentrations of KT and 2,4-D, their concentration decreasing from 0.1 to 0.01 mg·L^-1. Somatic embryos were successfully incubated in 1/2 macronutrient MSB suspension supplemented with 0.5 g· L^-1 glutamine and 0.5 g·L^-1 asparagine. Decrease in macronutrient concentration of MSB significantly alleviated browning and was beneficial to suspension cells. Transformation of somatic embryos into plants was induced in MSB medium supplemented with 3% sucrose, 0.5 g·L^-1 glutamine, 0.5 g·L^-1 asparagine, and 6.0 g·L^-1 agar. The effect of sucrose as carbohydrate was better than that of glucose for plant germination. Using this protocol, regenerated plantlets from the CCRI521 and Zhongzhi86-6 reached to as much as 19.6 and 18.5% somatic embryos, respectively.

关键词： Gossypium hirsutum L. recalcitrant genotypes plant regeneration somatic embryogenesis effect of macronutrients suspension culture