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Effect of 5-Aza-2’-deoxycytidine on immune-associated proteins in exosomes from hepatoma

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World Journal of gastroenterology 2010年第19期16卷 2371-2377页

作者： gao-wa Sanren Department of Histology and Embryology Inner Mongolia Medical College Hohhot 010010 Inner Mon-golia Autonomous Region China

AIM: To study the effect of 5-Aza-2’-deoxycytidine (5-Aza-CdR) on heat shock protein 70 (HSP70), human leucocyte antigen-Ⅰ (HLA-Ⅰ) and NY-ESO-1 proteins in exosomes produced by hepatoma cells, Hepg2 and Hep3B. METHODS: Exosomes derived from Hepg2 and Hep3B cells treated with or without 5-aza-CdR were isolated and purified by ultrafiltration centrifugation and sucrose gradient ultracentrifugation. The number of exosomes was counted under electron microscope. Concentration of proteins in exosomes was measured by bicinchoninic acid protein assay. Expression of HSP70, HLA-Ⅰ and NY-ESO-1 proteins in exosomes was detected by Western blotting and immunoelectron microscopy. mRNA expression of p53 gene was detected by reverse transcription polymerase chain ***: The mRNA expression of p53 gene was increased in both hepatoma cell lines after treatment with 5-Aza-CdR. The number of exosomes and the concentration of total proteins in exosomes were increased signifi cantly after treatment with 5-aza-CdR (P g showed that the HSP70, HLA-Ⅰ and NY-ESO-1 proteins were increased in exosomes produced by both hepatoma cell lines. CONCLUSION: 5-aza-CdR, an inhibitor of DNA methyltransferase, can increase exosomes produced by hepatoma cells and immune-associated protein component of exosomes, which may be mediated by p53 gene upregulation and 5-Aza-CdR demethylation.

关键词： 5-Aza-2’-deoxycytidine Exosome Immu-nomolecule Hepatoma cell

Identities Involving Powers and Inverse of Binomial Coefficients

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Journal of Mathematical Research and Exposition 2011年第6期31卷 1021-1029页

作者： Wu Yun g ao wa Department of Mathematics College of Sciences and Technology Inner Mongolia University Inner Mongolia 010021 P. R. China

In this paper,we give several identities of finite sums and some infinite series involving powers and inverse of binomial coefficients,which extends the results of ***.

关键词： inverse of binomial coefficient identities stirling numbers

The use of Phanerochaete chrysosporium,Trichoderma harzianum and Trichoderma viride for biogas production

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Studies in Fungi 2020年第1期5卷 368-380页

作者： Mahmoud YA-g Awadalla OA Estafanous AN Etawy wa Tanta University Faculty of ScienceBotany and Microbiology DepartmentMycology Research Lab.Tanta 31527Egypt Agriculture Microbiology Department SoilWater and Environment Research InstituteAgriculture Research CenterCairoEgypt

The expanding cost of ordinary fuel in urban zones requires the investigation of other vitality *** exploitation of agricultural wastes for biogas production could protect our environment and can also solve emerging energy crises *** chrysosporium,Trichoderma virdie and Trichoderma harzianum were used to ferment rice straw and cattle dung for production of compost and *** yield was determined using displacement technique,using acidified water(1 N H_(2)SO_(4))to prevent solubilization of carbon dioxide contained in the *** results showed that the highest rate of biogas and methane were recorded with treated rice straw mixed with cattle dung where the amount of production from the total gas was 51.84 of biogas and 37.86 of methane per Liter(L)/Kilogram(Kg)of fermented ***,the results showed that the consumed biogas and methane related to volatile solids gave the highest amount with untreated rice straw(86.32 and 56.72 L/Kg respectively)followed by treated rice straw and untreated and treated mixtures of rice straw and cattle dung *** results also showed that the highest bacterial count was obtained from fungi treated rice straw mixed with cattle dung(125×10^(3)cells/g)followed by treated rice straw(32×10^(3)cells/g).This study confirms that ***,*** and *** significantly speed up the process of aerobic and anaerobic fermentation of rice straw in respect to biogas and methane production.

关键词： Aerobic fermentation Anaerobic fermentation Methane Rice straw

A building-based data capture and data mining technique for air quality assessment

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Frontiers of Environmental Science & Engineering 2011年第4期5卷 543-551页

作者： Ni SHENg U wa TANg Faculty of Management and Administration Macao University of Science and TechnologyMacaoChina Department of Civil and Environmental Engineering University of MacaoMacaoChina Dirccao dos Servicos de Proteccao Ambiental MacaoChina

Recently,a building-based air quality model system which can predict air quality in front of individual buildings along both sides of a road has been *** the Macao Peninsula as a case study,this paper shows the advantages of building-based model system in data capture and data *** with the traditional grid-based model systems with input/output spatial resolutions of 1-2 km,the building-based approach can extract the street configuration and traffic data building by building and therefore,can capture the complex spatial variation of traffic emission,urban geometry,and air *** non-homogeneous distribution of air pollution in the Macao Peninsula was modeled in a highspatial resolution of 319 receptors·km^(-2).The spatial relationship among air quality,traffic flow,and urban geometry in the historic urban area is *** study shows that the building-based approach may open an innovative methodology in data mining of urban spatial data for environmental *** results are particularly useful to urban planners when they need to consider the influences of urban form on street environment.

关键词： traffic air pollution spatial distribution high resolution geographic information system

Application of Shear wave Elastography and Contrast-Enhanced Ultrasound in Transrectal Prostate Biopsy

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Current Medical Science 2022年第2期42卷 447-452页

作者： gao-wa SHAREN Jun ZHANg Department of Ultrasound Affiliated Hospital of Inner Mongolia Medical UniversityHohhot010050China Department of Urology Affiliated Hospital of Inner Mongolia Medical UniversityHohhot010050China

Objective To explore the clinical value of ultrasound shear wave elastography(SWE)and contrast-enhanced ultrasound(CEUS)in transrectal prostate *** A total of 54 patients(average age:67.79±12.01 years)in the experimental group underwent transrectal prostate biopsy under the guidance of SWE,while 46 patients(average age:69.22±11.54 years)in the control group underwent transrectal prostate biopsy guided by *** There were a total of 451 needles,with an average of 8.35±1.67 needles per patient in the experimental group,and a total of 462 needles,with an average of 10.04±1.33 needles per patient in the control *** difference in puncture times between the two groups was statistically significant(Pwas no significant difference in the positive detection rate,sensitivity or specificity between the two groups(P>0.05),but there was a significant difference in the diagnostic accuracy between the two groups(Pgnificantly higher in the experimental group than in benign prostatic hyperplasia(Pg characteristic curve(ROC)analysis showed that the area under the ROC curve(AUC)of E_(mean)was 0.752(S.E.=0.072,95%CI=0.611-0.894,P=0.007),and the best cutoff value was 47.005 *** In summary,both SWE-and CEUS-guided transrectal prostate biopsy can help find the focus and guide the puncture,and improve the positive detection rate.

关键词： prostate puncture ultrasonic shear wave elastography contrast-enhanced ultrasound biopsy analysis

Advances in photothermal conversion of carbon dioxide to solar fuels

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Journal of Energy Chemistry 2023年第8期83卷 62-78,I0004页

作者： wa.gao yinwen Li Dequan Xiao Ding Ma College of Bioscience and Resources Environment Beijing University of AgricultureBeijing 102206China College of Chemistry and Molecular Engineering and College of Engineering Peking UniversityBeijing 100871China Center for Integrative Materials Discovery Department of Chemistry and Chemical and Biomedical EngineeringUniversity of New HavenWest HavenCT 06516USA

Converting carbon dioxide(CO_(2)) into useful fuels or chemical feedstocks is important for achieving peak carbon emission and carbon ***,photothermal catalysis has been extensively studied and applied due to its advantages over traditional heat-driven *** this review,we focus on photothermal catalysis of thermodynamically uphill reactions that convert CO_(2)into value-added *** first introduce the fundamentals of photothermal catalysis for CO_(2)reduction,including the definition and classification of photothermal catalysis,followed by their photothermal conversion *** structure design of different types of photothermal catalysts is *** superior performance of photothermal catalytic conversion of CO_(2)is illustrated and discussed,including improved CO_(2)activation,tunable selectivity towards different solar fuel products,and resistance to sintering and ***,the perspectives and challenges in this cutting-edge field are presented with the aim of advancing understanding of the underlying mechanisms and inspiring rational design of photothermal catalysts for highly efficient solar-to-fuel conversion.

关键词： Solarenergy Photothermal catalysis CO_(2)reduction Catalyst design Performance improvement

Nanostructured Catalyst for Fischer-Tropsch Synthesis

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Chinese Journal of Chemistry 2018年第9期36卷 798-808页

作者： wa.gao Qingshan Zhu Ding Ma College of Chemistry and Molecular Engineering and College of Engineering Peking University Beijing 100871 China Fritz-Haber-Institut der Max-Planck-gesellschaft Faradayweg 4-6 D-14195 Berlin Germany

Fischer-Tropsch synthesis （FTS） is a heterogeneous catalytic process for the production of fuels or chemicals from synthesis gas （CO ＋ H2）, which can be derived from nonpetroleum feedstocks such as natural gas, coal, or biomass. Co, Ru, Fe and Ni are all active in FTS, but only cheaper Fe and Co based catalysts are used in industry because the price of Ru is relatively high. However, the industrial Fe- and Co-based FTS catalysts normally work at a relatively high temperature range of 493--623 K in order to get a reasonable space time yield. Moreover, the product selectivity of FTS is governed by the law of polymerization, i.e., a so-called Anderson-Schulz-FIory distribution holds, which restricts its industrial application. In this account, we highlight some of our progress toward the design/fabrication of nanostructured Fe, Co and Ru catalysts to improve FTS activity at the low temperature and to change the product selectivity and confine the product distribution into a certain range.

关键词： catalyst Fischer-Tropsch synthesis nanostructure

Capture of cervical exfoliative cells on a glass slide coated by 3-glycidyloxypropyl trimethoxysilane and poly-L-lysine

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Journal of Pharmaceutical Analysis 2012年第3期2卷 174-179页

作者： gao-wa Aodeng School of Chemistry and Chemical Engineering Inner Mongolia University

A new modification method for glass slides was developed and applied to make ThinPrep Pap smears,in order to increase the adhesion ability of cervical exfoliative cells.3-glycidyloxypropyl trimethoxysilane(gOPS) was coated on the glass slides firstly on the slides,then poly-L-lysine(PLL) was covalently modified onto the above epoxy-terminated slides to form gOPS-PLL double decorated *** modified slides were characterized using X-ray photoelectron spectroscopy(XPS) and atomic force microscopy(AFM).The cell adhesion ability effect was tested and compared with traditional PLL coated slides by fixing the cervical exfoliative cells on the double adorned *** control test was conducted by the bare glass slides *** cell morphology of cervical exfoliative cells adhered on different slides was observed under the microscope after Papanicolaou *** number of cervical exfoliative cells on the unmodified slides,PLL coated slides and gOPS-PLL coated slides was 10307300,32837226 and 41197280(n=12),*** data among the three different modification methods showed significant differences(one-way analysis of variance,ANOVA test,Po0.05).The cell capturing effect of the gOPS-PLL slide was the best among the three different modified *** addition,the gOPS-PLL slide could enhance the uniformity of the adhered cells and be widely applied to the ThinPrep system for cervical carcinoma screening to increase the accuracy rate of diagnosis.

关键词： glass slide Cervical exfoliative cells ThinPrep Pap smear

Microscopic Structures of Endosperms Before and After gelatinization in Rice Varieties with Varied grain Quality

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Agricultural Sciences in China 2003年第1期2卷 113-118,F003,T002页

作者： YANgZe－min wa.gWei－jin LANSheng－yin XUZhen－xiu ZHOUZhu－qing wa CollegeofLifeSciemceandTechnology HuazhongAgriculturalUniversityWuhan430070P.R.China CollegeofPlantScienceandTechnology Wuhan430070P.R.China

The microscopic structures of the endosperm of indica rice varieties with different quality before and after gelatinization were observed using scanning electron microscope. The results showed that the degree of gelatinization varied in different parts of the grain and in different varieties under the same experimental conditions. The gelatinization of dorsal side was the most complete. Its cells were decomposed totally into puff-like or flocculent materials. The ventral side gelatinized less thoroughly, appearing agglomerate and some cell frames were still visible. The middle part gelatinized most incompletely and the cells were still integrated. Evident differences in gelatinization were observed among different varieties, the dorsal, ventral and middle parts of high quality varieties gelatinized more thoroughly than those of the corresponding parts of low quality varieties respectively. An obvious concavity often appeared in the middle of the cross-section of the low quality grains while the cross-section of high quality grains was normally flat. The same phenomenon was noted when comparing the early maturing indica rice and the late maturing indica rice. Varietal difference of gelatinization in dorsal sides was not as distinct as in middle parts and ventral sides. The difference among dorsal side, middle part and ventral side in gelatinization was greater in low quality grains than that of high quality grains. In addition, a lot of ruptured cells were observed in the cross-section of high quality rice, while few of them could be found in the low quality rice. Apparently, the number of ruptured cells is positively correlated with rice quality. Quality of rice grain also has positive correlation with the rate of water absorption and extension. High rates of water absorption and extension lead to better gelatinization of rice grain, and hence indicate good quality.

关键词： Indica rice grain quality gelatinization Microscopic structure