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Temporal and environmental factors drive community structure and function of methanotrophs in volcanic forest soils

Journal of Forestry Research 2024年第2期35卷 1-13页

作者： Rusong Chai Hongjie cao Qingyang Huang Lihong Xie Fan Yang Hongbin yin Heilongjiang Forest Botanical Garden Harbin 150040People’s Republic of China 2 Institute of Natural Resources and Ecology Science Academy of Heilongjiang ProvinceHarbin 150040People’s Republic of China

Methanotrophs,organisms that obtain oxygen by oxidizing methane,are recognized as the only known biological sink for atmospheric CH_4,and forest soil methanotrophs play crucial roles in mitigating global warming.The succession patterns of methanotrophic communities and functions in Wudalianchi volcano forest soils could provide a basis for the study of evolutionary mechanisms between soil microorganisms,the environment,and carbon cycling of temperate forest ecosystems under climate change.In this study,the characteristics and drivers of methanotrophic community structure and function of two volcanic soils at different stages of development are analyzed,including an old volcano and a new volcano,which most recently erupted 300 years and 17-19×10^(5)years ago,respectively,and a non-volcano hills as control,based on space for time substitution and Miseq sequencing and bioinformation technology.The results showed that CH_(4) fluxes were significantly higher in old-stage volcano forest soils than new-stage forest soils and non-volcano forest soils.There were significant differences in the community composition and diversity of soil methanotrophs from different volcano forest soils.Methylococcus was the dominant genus in all soil samples.Additionally,the relative abundance of Methylococcus,along with Clonothrix,Methyloglobulus,Methylomagum,Methylomonas and Methylosarcina,were the important genera responsible for the differences in methanotrophic community structure in different volcano forest soils.The relative abundance of methanotroph belonging toγ-proteobacteria was significantly higher than that belonging toα-proteobacteria(P<0.05).Chao1,Shannon and Simpson indices of soil methanotrophic community were significantly lower in new-stage volcanos and were significantly affected by bulk density,total porosity,p H,nitrate,dissolved organic carbon and dissolved organic nitrogen.There were significant differences in community structure between new-stage and old-stage volcanoes.Bulk density and p H are important soil prope

关键词： Methanotrophs pmo A Soil development stage Volcanoes Forest soils

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Flashover Characteristics of Dry Band-water band on Hydrophobic Surfaces

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CSEE Journal of Power and Energy Systems 2024年第1期10卷 392-400页

作者： Xiaohan Wu Fanghui yin Bin cao Daiming Yang Shichao Shao Liming Wang Tsinghua-Berkeley Shenzhen Institute(TBSI) Shenzhen 518055China Shenzhen International Graduate School Tsinghua UniversityShenzhen 518055China Shenzhen Polytechnic School Shenzhen 518055China

Due to their outstanding anti-flashover characteristics,composite insulators have been extensively applied in power systems.A lot of research has investigated flashover characteristics of hydrophobic specimens with artificial water droplets.However,the phenomenon is not consistent with that of the contaminated composite insulators.On the test specimens covered with water droplets,there is no obvious leakage current before the flashover and no obvious relationship between flashover voltage and the conductivity of water droplets.On the contaminated composite insulator surface,there are short continuous arcs on the insulators before critical flashover,making insulators look like a luminous lantern.Considering that under these two conditions,the proportion of water along the insulation distance is different,the flashover characteristic of“dry band-water band”on a hydrophobicity surface is analyzed in the present study.The influence of the water band parameter(including length,width,and conductivity)as well as the length of dry band is studied.On this basis,the arc generation and development process of the surface covered with“dry band-water band”is analyzed.The research results improve the understanding of the flashover process on contaminated composite insulators.

关键词： Flashover characteristics hydrophobicity partial arc water band

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Preparation and performance improvement of a dual-component microcapsule self-healing system for silicone rubber insulating material

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High Voltage 2024年第2期9卷 453-465页

作者： Mingzhe Li Bin cao Fanghui yin Hongwei Mei Liming Wang Engineering Laboratory of Power Equipment Reliability in Complicated Coastal Environments Tsinghua Shenzhen International Graduate SchoolTsinghua UniversityShenzhenChina

A key factor limiting the development of silicone rubber insulating materials is their short service life in harsh operating environments.The establishment of a self-healing system is desired to improve the long-term operational performance of silicone rubber and effectively extend its service life.In this study,poly(urea-formaldehyde)microcapsules containing polydimethylsiloxane healing agents are prepared and a dual-component microcapsule self-healing system for silicone rubber material is established and opti-mised.In addition,the key factors affecting the self-healing performance(such as microcapsule size,rupture rate,and microcapsule doping amount)are investigated.It is found that when SYLGARDTM 184 is used as the healing agent and matrix,with a 4:6(A:B)doping ratio of dual-component microcapsules and 20 phr doping amount,the self-healing efficiency is approximately 51%.Furthermore,increasing the microcapsule rupture rate and the interface strength between the healing agent and the matrix can effectively improve the self-healing performance.The relationships between key factors and self-healing performance are theoretically revealed.Based on the relationships,the microcapsule self-healing system can be appropriately designed.The results of this paper can provide a reference for the development of long-life silicone rubber insulating materials.

关键词： service rubber performance

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Prediction and driving factors of forest fire occurrence in Jilin Province,China

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Journal of Forestry Research 2024年第1期35卷 58-71页

作者： Bo Gao Yanlong Shan Xiangyu Liu Sainan yin Bo Yu Chenxi Cui Lili cao Wildland Fire Prevention and Fighting Innovation Center of Beihua University Forestry College of Beihua University3999 Binjiang East RoadJilin City 132013People’s Republic of China

Forest fires are natural disasters that can occur suddenly and can be very damaging,burning thousands of square kilometers.Prevention is better than suppression and prediction models of forest fire occurrence have developed from the logistic regression model,the geographical weighted logistic regression model,the Lasso regression model,the random forest model,and the support vector machine model based on historical forest fire data from 2000 to 2019 in Jilin Province.The models,along with a distribution map are presented in this paper to provide a theoretical basis for forest fire management in this area.Existing studies show that the prediction accuracies of the two machine learning models are higher than those of the three generalized linear regression models.The accuracies of the random forest model,the support vector machine model,geographical weighted logistic regression model,the Lasso regression model,and logistic model were 88.7%,87.7%,86.0%,85.0%and 84.6%,respectively.Weather is the main factor affecting forest fires,while the impacts of topography factors,human and social-economic factors on fire occurrence were similar.

关键词： Forest fire Occurrence prediction Forest fire driving factors Generalized linear regression models Machine learning models

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Responses of Nutrients to the Precipitation Variation and Land Use in Subtropical Monsoonal Small Mountainous Rivers:A Case Study of Baixi Watershed

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Journal of Ocean University of China 2024年第2期23卷 383-391页

作者： TIAN Yuan GAO Fei cao Ke LV Shenghua DUAN Xiaoyong yin Ping Qingdao Institute of Marine Geology China Geological SurveyMinistry of Natural ResourcesQingdao 266237China Laboratory for Marine Geology Laoshan LaboratoryQingdao 266237China

Small mountainous rivers are characterized by large instantaneous fluxes and susceptible to extreme weather events,which can rapidly transport materials into the sea and have a significant impact on the ecological environment of estuaries and bays.In order to investigate the seasonal characteristics of nutrients in small mountainous rivers in the subtropical monsoon region and the output pattern to the sea during heavy precipitation,surveys on the mountainous rivers were carried out in Baixi watershed in August 2020(wet season),March 2021(dry season)and June 2021(Meiyu period).The results showed that the dissolved inorganic nitrogen(DIN)of the rivers has an average concentration of 752μg L^(−1)in the wet season and 1472μg L^(−1)in the dry season.The concentrations of dissolved inorganic phosphorus(DIP)in wet season and dry season were 63μg L^(−1)and 51μg L^(−1),respectively.Influenced by the changes of land use in sub-watersheds,DIN concentrations in the mainstream increased from 701μg L^(−1)in the upper reaches to 1284μg L^(−1)in the middle reaches.Two rainstorms during the Meiyu period in the watershed caused the pulse runoff in the river.The maximum daily runoff reached 70 times that before rains.The maximum daily fluxes of DIN and DIP were 109 and 247 times that before rains,respectively.In view that the watershed experienced several rainstorms in the wet season,the river,with pulse runoff,carries a large amount of nutrients into the sea in a short time,which will have a significant impact on the environment of Sanmen bay and its adjacent sea.

关键词： small mountainous river uneven precipitation rainstorm pulse runoff nitrogen and phosphorus

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Effects of mesoclimate and microclimate variations mediated by high altitude and row orientation on sucrose metabolism and anthocyanin synthesis in grape berries

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Horticultural Plant Journal 2024年第3期10卷 713-731页

作者： Haining yin Zhaoxiang Wang Lin Wang Jianhong cao Jiakui Wang Zhumei Xi College of Enology Northwest A&F UniversityYanglingShaanxi 712100China Shangri-la Winery Co. LTDShangri-laYunnan 674400China

Climate change and extreme weather pose significant challenges to the traditional viticulture regions.Emerging high-altitude grape-producing regions with diverse orientations have shown great potential in coping with this challenge.Stable,high-quality wine grape production may be achieved by synchronizing the meso-and microclimate.To clarify the role of high altitude and row orientation in meso-and microclimate and the response of berries to it,we evaluated seven years(2012-2018)of climate data,two years of basic grape(Cabernet Sauvignon,Vitis vinifera L.)quality,and one-year microclimate from veraison to harvest.By comparing two locations(Sidon 2047 m,Sinon 2208 m)in Yunnan Province,China,we found that the average temperature has been stable at approximately 15℃ for seven years,with no extreme weather or,noticeable global warming.The light intensity(LI)in the north-south(NS)was more balanced than the east-west(EW)direction,and the east-west to the south(EW-S)canopy side was almost higher than the other sides.High LI was associated with high photosynthetically active radiation(PAR),ultraviolet(UV),and infrared(IR)light and vice versa.The north-south to the east(NS-E)and east-west to the north(EWN)sides were characterized by lower LI and higher UV and IR light,and higher total anthocyanin content.Most anthocyanin synthesis-related genes,for example,VvF3'H and VvF3'5'H,were highly expressed in NS-E from veraison to maturity.Perhaps UV and IR light induced their expression.This study provides new insights on the role of differently orientated rows in controlling grape quality due to varied light quality.The findings are globally significant,particularly in the context of climate change,and offer fresh insights into berry physiological responses and decision-making for the management of existing vineyards.

关键词： Vitis vinifera L. Orientated rows Berry composition Sugar Phenolic Light quality Solar radiation

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Ferroptosis contributes to JEV-induced neuronal damage and neuroinflammation

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Virologica Sinica 2024年第1期39卷 144-155页

作者： Wenjing Zhu Qi Li Yong yin Huanchun Chen Youhui Si Bibo Zhu Shengbo cao Zikai Zhao Jing Ye National Key Laboratory of Agricultural Microbiology Huazhong Agricultural UniversityWuhan430070China Frontiers Science Center for Animal Breeding and Sustainable Production Huazhong Agricultural UniversityWuhan430070China The Cooperative Innovation Center for Sustainable Pig Production Huazhong Agricultural UniversityWuhan430070China Hubei Hongshan Laboratory Wuhan430070China

Ferroptosis is a newly discovered prototype of programmed cell death (PCD) driven by iron-dependent phospholipid peroxidation accumulation, and it has been linked to numerous organ injuries and degenerative pathologies. Although studies have shown that a variety of cell death processes contribute to JEV-induced neuroinflammation and neuronal injury, there is currently limited research on the specific involvement of ferroptosis. In this study, we explored the neuronal ferroptosis induced by JEV infection in vitro and in vivo. Our results indicated that JEV infection induces neuronal ferroptosis through inhibiting the function of the antioxidant system mediated by glutathione (GSH)/glutathione peroxidase 4 (GPX4), as well as by promoting lipid peroxidation mediated by yes-associated protein 1 (YAP1)/long-chain acyl-CoA synthetase 4 (ACSL4). Further analyses revealed that JEV E and prM proteins function as agonists, inducing ferroptosis. Moreover, we found that treatment with a ferroptosis inhibitor in JEV-infected mice reduces the viral titers and inflammation in the mouse brains, ultimately improving the survival rate of infected mice. In conclusion, our study unveils a critical role of ferroptosis in the pathogenesis of JEV, providing new ideas for the prevention and treatment of viral encephalitis.

关键词： Japanese encephalitisvirus(JEV) Ferroptosis Neuron Lipid peroxidation Inflammatory response

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Study on the influence of mechanism dispersion on transient recovery voltage distribution of modular DC vacuum circuit breakers

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High Voltage 2024年第2期9卷 346-355页

作者： Chongyang Huang Yongshuai yin Shuxin Liu Hanwen Du Zengyu Shan Yundong cao Shurui Yang Key Laboratory of Special Electric Machines and High Voltage Apparatus in the Ministry of Education Shenyang University of TechnologyShenyangChina Shenyang Power Supply Company of State Grid Liaoning Electric Power Co. LtdShenyangChina Chifeng Power Supply Company of State Grid Mongolia Electric Power Co. LtdChifengChina

A simulation analysis and an experiment are carried out to investigate how the gap difference between the breaks of a direct current vacuum circuit breakers with multi-breaks(MB-DC VCB)caused by the mechanism dispersion of the breaker influences the distribution of TRV among the breaks.An interruption model of MB-DC VCB,combining the continuous transition model,is established to analyse the rising rate of transient recovery voltage and the dielectric strength recovery speed of the breaks for MB-DC VCB under different gap difference conditions.Based on the experimental platform of dual break DC vacuum circuit breaker breaking,the correctness of the simulation model is verified on a DC VCB with the double-breaks interruption experimental platform.Moreover,a model is applied to the non-synchronous interruption simulation of a DC VCB with three-breaks.The relationship between the TRVs of the breaks under different gap difference conditions is analysed using the comparative analysis method,obtaining the maximum gap difference at the moment of breaking failure.The results of this study show that large-gap breaks have a higher TRV than small-gap breaks(the fracture of the action delay module),with double fractures reaching 1.4 times and triple fractures reaching a maximum of 1.52 times;the ability of small-gap breaks to withstand TRV is weak,giving rise to re-breakdown or even interruption failure;as the number of fractures increases,the maximum gap difference also increases.Improving the synchronous interruption ability of the MB-DC VCB is conducive to improving the interruption performance and interruption success rate of this type of a circuit breaker.

关键词： TRV transient distribution

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Synthesis of porous iron hydroxy phosphate from phosphate residue and its application as a Fenton-like catalyst for dye degradation

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Journal of Environmental Sciences 2022年第2期34卷 307-319页

作者： Weilin Guo yin cao Yi Zhang Lijun Wang Lingxue Kong School of Environmental and Materials Engineering Shanghai Polytechnic UniversityShanghai 200240China Deakin University GeelongInstitutefor Frontier MaterialsVictoria 3216Australia Shanghai Innovation Institutefor Materials Shanghai 200444China

Phosphate residue is a kind of hazardous solid waste and if not properly disposed of, could cause serious environmental contaminations. The abundant iron salt available in phosphate residue can be used to prepare photo-Fenton catalytic reagent for wastewater treatment. In this study, the phosphate residue was effectively purified by a hydrothermal recrystallization method, reaching an iron phosphate purity of 94.2%. The particles of iron phosphate were further processed with ball milling with their average size reduced from 19.4 to 1.6 μm. By hydrothermal crystallization of iron phosphate and thermal decomposition of oxalate precursor, porous iron hydroxy phosphate was prepared. The modified porous iron hydroxy phosphate(m-PIHP) of higher surface area with iron oxalate on its surface can degrade 98.87% of Rhodamine B in 15 min. Cyclic experiment showed that the catalyst still had a good catalytic activity after six cycles( > 40%). The X-ray photoelectron spectroscopy results showed that the iron oxalate complex on the catalyst surface decomposed to produce ferrous ions and accelerated the rate of · OH production. The current work demonstrated that the m-PIHP synthesized from phosphate residue and modified with iron oxalate can be used as an effective dye wastewater treatment agent.

关键词： Phosphate residue Porous iron hydroxy phosphate Fenton-like ·OH

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Geometry and formation mechanism of tension gashes and their implication on the hydrocarbon accumulation in the deep-seated strata of sedimentary basin:A case from Shunnan area of Tarim Basin

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Petroleum Science 2024年第1期21卷 87-99页

作者： Yan-Nan Du Kong-You Wu yin Liu Yan-ying Li Zi-Cheng cao You-Wei Cui Jun Liu Key Laboratory of Deep Oil and Gas School of GeosciencesChina University of Petroleum(East China)Qingdao 266580ShandongChina Exploration and Development Research Institute SINOPEC Northwest Oilfield BranchUrumqi 830011XinjiangChina The Fifth Gas Production Plant Changqing OilfieldPetroChinaXi'an 710018ShaanxiChina

With the theoretical and technological developments related to cratonic strike-slip faults,the Shuntuoguole Low Uplift in the Tarim Basin has attracted considerable attention recently.Affected by multi-stage tectonic movements,the strike-slip faults have controlled the distribution of hydrocarbon resources owing to the special fault characteristics and fault-related structures.In contrast,the kinematics and formation mechanism of strike-slip faults in buried sedimentary basins are difficult to investigate,limiting the discussion of these faults and hydrocarbon accumulation.In this study,we identified the characteristics of massive sigmoidal tension gashes(STGs)that formed in the Shunnan area of the Tarim Basin.High-resolution three-dimensional seismic data and attribute analyses were used to investigate their geometric and kinematic characteristics.Then,the stress state of each point of the STGs was calculated using seismic curvature attributes.Finally,the formation mechanism of the STGs and their roles in controlling hydrocarbon migration and accumulation were discussed.The results suggest that:(1)the STGs developed in the Shunnan area have a wide distribution,with a tensile fault arranged in an enéchelon pattern,showing an S-shaped bending.These STGs formed in multiple stages,and differential rotation occurred along the direction of strike-slip stress during formation.(2)Near the principal displacement zone of the strike-slip faults,the stress value of the STGs was higher,gradually decreasing at both ends.The shallow layer deformation was greater than the deep layer deformation.(3)STGs are critical for connecting source rocks,migrating oil and gas,sealing horizontally,and developing efficient reservoirs.This study not only provides seismic evidence for the formation and evolution of super large STGs,but also provides certain guidance for oil and gas exploration in this area.

关键词： Tarim Basin Sigmoidal tension gashes Seismic attributes Shear stress calculation Formation mechanism Reservoir control

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