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Two-dimensional coordination polymer-based nanosensor for sensitive and reliable nucleic acids detection in living cells

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Chinese Chemical Letters 2022年第2期33卷 968-972页

作者： Yuzhi Xu Yanfei Zhang Huihui Yang Wen Yin Leli Zeng Shuo Fang Si-Yang Liu Zong Dai Xiaoyong Zou yihang pan Precision Medicine Center Scientific Research CenterThe Seventh Affiliated HospitalSun Yat-sen UniversityShenzhen 518107China Key Laboratory of Sensing Technology and Biomedical Instrument of Guangdong Province School of Biomedical EngineeringSun Yat-sen UniversityGuangzhou 510006China School of Chemistry Sun Yat-sen UniversityGuangzhou 510275China Department of Oncology The Seventh Affiliated HospitalSun Yat-sen UniversityShenzhen 518107China

A reliable and sensitive strategy which can assess nucleic acid levels in living cells would be essential for fundamental research of biomedical applications. Some nanomaterial-based fluorescence biosensors recently developed for detecting nucleic acids, however, are often with expensive, complicated and timeconsuming preparation process. Here, by using a facile bottom-up synthesis method, a two-dimensional(2 D) coordination polymer(CP) nanosheet, [Cu(tz)](Htz = 1,2,4-triazole), was successfully prepared after optimizing reaction conditions. These ultrathin CP nanosheets with thickness of 4.7 ± 1.1 nm could readily form nanosensors by assembly with DNA probes, which exhibited a low limit of detection(LOD)for p53 DNA fragment as 144 pmol/L. Furthermore, by integrating [Cu(tz)] nanosheets with hybridization chain reaction(HCR) probes, mi R-21, one kind of micro RNA upregulated in many cancer cells, can be sensitively detected with a LOD of 100 pmol/L and monitored in living cells, giving consistent results with those obtained by quantitative reverse-transcription polymerase chain reaction(q RT-PCR) *** [Cu(tz)] nanosheets, which not only possess much better nucleic acids sensing performance than bulk cystals, but also exhibit nucleic acid delivery functions, could be used as a novel nanoplatform in biomedical imaging and sensing applications.

关键词： Coordination polymers Nanosheets Fluorescence biosensor Nucleic acids detection Living cells

Signaling pathways and therapeutic interventions in gastric cancer

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Signal Transduction and Targeted Therapy 2022年第11期7卷 4118-4155页

作者： Zi-Ning Lei Qiu-Xu Teng Qin Tian Wei Chen Yuhao Xie Kaiming Wu Qianlin Zeng Leli Zeng yihang pan Zhe-Sheng Chen Yulong He Guangdong Provincial Key Laboratory of Digestive Cancer Research Digestive Diseases CenterScientific Research CenterThe Seventh Affiliated Hospital of Sun Yat-Sen University518107ShenzhenGuangdongChina Department of Pharmaceutical Sciences College of Pharmacy and Health SciencesSt.John’s UniversityQueensNY11439USA Institute for Biotechnology St.John’s UniversityQueensNY11439USA

Gastric cancer(GC)ranks fifth in global cancer diagnosis and fourth in cancer-related *** tremendous progress in diagnosis and therapeutic strategies and significant improvements in patient survival,the low malignancy stage is relatively asymptomatic and many GC cases are diagnosed at advanced stages,which leads to unsatisfactory prognosis and high recurrence *** the recent advances in genome analysis,biomarkers have been identified that have clinical importance for GC diagnosis,treatment,and *** molecular classifications have uncovered the vital roles that signaling pathways,including EGFR/HER2,p53,PI3K,immune checkpoint pathways,and cell adhesion signaling molecules,play in GC tumorigenesis,progression,metastasis,and therapeutic *** biomarkers and molecular classifications open the way for more precise diagnoses and treatments for GC ***,the relative significance,temporal activation,interaction with GC risk factors,and crosstalk between these signaling pathways in GC are not well ***,we review the regulatory roles of signaling pathways in GC potential biomarkers,and therapeutic targets with an emphasis on recent *** therapies,including signaling-based and immunotherapies exploited in the past decade,and the development of treatment for GC,particularly the challenges in developing precision medications,are *** advances provide a direction for the integration of clinical,molecular,and genomic profiles to improve GC diagnosis and treatments.

关键词： diagnosis therapeutic precise

Synergistic effects of autophagy/mitophagy inhibitors and magnolol promote apoptosis and antitumor efficacy

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Acta Pharmaceutica Sinica B 2021年第12期11卷 3966-3982页

作者： Yancheng Tang Liming Wang Tao Yi Jun Xu Jigang Wang Jiang-Jiang Qin Qilei Chen Ka-Man Yip yihang pan Peng Hong Yingying Lu Han-Ming Shen Hu-Biao Chen School of Chinese Medicine Hong Kong Baptist UniversityHong Kong SAR 999077China School of Biomedical Sciences Hunan UniversityChangsha 410082China Department of Physiology Yong Loo Lin School of MedicineNational University of SingaporeSingapore 117597Singapore Artemisinin Research Center China Academy of Chinese Medical SciencesBeijing 100700China The First Affiliated Hospital of Southern University of Science and Technology the Second Clinical Medical College of Jinan UniversityShenzhen People’s HospitalShenzhen 518020China The Cancer Hospital of the University of Chinese Academy of Sciences(Zhejiang Cancer Hospital) Institute of Basic Medicine and Cancer(IBMC)Chinese Academy of SciencesHangzhou 310022China epartment of Medical Research Seventh Affiliated HospitalSun Yat-sen UniversityShenzhen 518107China Department of Biomedical Science City University of Hong KongHong Kong SAR 999077China Faculty of Health Sciences University of MacaoMacao SAR 999078China

Mitochondria as a signaling platform play crucial roles in deciding cell *** classic anticancer agents are known to trigger cell death through induction of mitochondrial ***,one selective autophagy,is the key mitochondrial quality control that effectively removes damaged ***,the precise roles of mitophagy in tumorigenesis and anticancer agent treatment remain largely ***,we examined the functional implication of mitophagy in the anticancer properties of magnolol,a natural product isolated from herbal Magnolia ***,we found that magnolol induces mitochondrial depolarization,causes excessive mitochondrial fragmentation,and increases mitochondrial reactive oxygen species(mtROS).Second,magnolol induces PTEN-induced putative kinase protein 1(PINK1)-Parkin-mediated mitophagy through regulating two positive feedforward amplification ***,magnolol triggers cancer cell death and inhibits neuroblastoma tumor growth via the intrinsic apoptosis ***,magnolol prolongs the survival time of tumor-bearing ***,inhibition of mitophagy by PINK1/Parkin knockdown or using inhibitors targeting different autophagy/mitophagy stages significantly promotes magnolol-induced cell death and enhances magnolol's anticancer efficacy,both in vitro and in ***,our study demonstrates that magnolol can induce autophagy/mitophagy and apoptosis,whereas blockage of autophagy/mitophagy remarkably enhances the anticancer efficacy of magnolol,suggesting that targeting mitophagy may be a promising strategy to overcome chemoresistance and improve anticancer therapy.

关键词： PINK1-Parkin-mediated mitophagy Magnolol Combination therapy Apoptosis Tumor suppression

Modulating the function of ABCB1: in vitro and in vivo characterization of sitravatinib, a tyrosine kinase inhibitor

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Cancer Communications 2020年第7期40卷 285-300页

作者： Yuqi Yang Ning Ji Chao-Yun Cai Jing-Quan Wang Zi-Ning Lei Qiu-Xu Teng Zhuo-Xun Wu Qingbin Cui yihang pan Zhe-Sheng Chen Department of Pharmaceutical Sciences College of Pharmacy and Health SciencesSt.John’s UniversityQueensNew York 11439USA State Key Laboratory of Experimental Hematology Chinese Academy of Medical Science and Peking Union Medical CollegeInstitute of Hematology and Blood Diseases HospitalTianjin 300020P.R.China School of Public Health Guangzhou Medical UniversityGuangzhouGuangdong 511436P.R.China Tomas Lindahl Nobel Laureate Laboratory the Seventh Affiliated Hospital of Sun Yat-sen UniversityShenzhenGuangdong 518107P.R.China

Background:Overexpression of ATP-binding cassette(ABC)transporter is a major contributor to multidrug resistance(MDR),in which cancer cells acquire resistance to a wide spectrum of chemotherapeutic *** this work,we evaluated the sensitizing effect of sitravatinib,a broad-spectrum tyrosine kinase inhibitor(TKI),on ATP-binding cassette subfamily B member 1(ABCB1)-and ATP-binding cassette subfamily C member 10(ABCC10)-mediated ***:MTT assay was conducted to examine cytotoxicity and evaluate the sensitizing effect of sitravatinib at non-toxic ***-labeled paclitaxel transportation,Western blotting,immunofluorescence analysis,and ATPase assay were carried out to elucidate the mechanism of sitravatinib-induced *** in vitro findings were translated into preclinical evaluation with the establishment of xenograft ***:Sitravatinib considerably reversed MDR mediated by ABCB1 and partially antagonized ABCC10-mediated *** in silico docking simulation analysis indicated that sitravatinib strongly and stably bound to the transmembrane domain of ABCB1 human-mouse chimeric ***,sitravatinib inhibited hydrolysis of ATP and synchronously decreased the efflux function of ***,sitravatinib could considerably enhance the intracellular concentration of anticancer ***,no significant alterations of both expression level and localization of ABCB1 were *** importantly,sitravatinib could remarkably restore the antitumor activity of vincristine in ABCB1-mediated xenograft model without observable toxic ***:The findings in this study suggest that the combination of sitrvatinib and substrate antineoplastic drugs of ABCB1 could attenuate the MDR mediated by the overexpression of ABCB1.

关键词： Sitravatinib

THE TELECONNECTION BETWEEN TYPHOON FREQUENCY ON WESTERN PACIFIC AND THE SEA SURFACE TEMPERATURE OF EASTERN EQUATORIAL PACIFIC

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Chinese Science Bulletin 1981年第2期26卷 157-160页

作者：潘怡航 Institute of Atmospheric Physics Academia Sinicayphoon is the severe synoptical system generated from the tropical ocean surface and often brings disastrous weather to the coastal area of our country. Therefore it is necessary to study the rule governing its genesis and development and to derive finally prediction technique. Since the energy of its development comes mainly from the sensible and latent heat over the ocean surface there must exist certain con-

Typhoon is the severe synoptical system generated from the tropical ocean surface and often brings disastrous weather to the coastal area of our country. Therefore, it is necessary, to study the rule governing its genesis and development, and to derive finally prediction technique. Since the energy of its development comes mainly from the sensible and latent heat over the ocean surface, there must exist certain con-

关键词： ocean coastal latent SURFACE weather genesis comes typhoon tropical finally

Inhibition of Mcl-1 enhances cell death induced by the Bcl-2-selective inhibitor ABT-199 in acute myeloid leukemia cells

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Signal Transduction and Targeted Therapy 2017年第1期2卷 242-250页

作者： Daniel A Luedtke Xiaojia Niu yihang pan Jianyun Zhao Shuang Liu Holly Edwards Kang Chen Hai Lin Jeffrey W Taub Yubin Ge Cancer Biology Graduate Program Wayne State University School of MedicineDetroitMichiganUSA National Engineering Laboratory for AIDS Vaccine School of Life SciencesJilin UniversityChangchunChina Department of Pathology and Laboratory Medicine Weill Cornell MedicineNew YorkNew YorkUSA Department of Pediatrics Wayne State University School of MedicineDetroitMichiganUSA Department of Oncology Wayne State University School of MedicineDetroitMichiganUSA Molecular Therapeutics Program Karmanos Cancer InstituteWayne State University School of MedicineDetroitMichiganUSA Department of Obstetrics and Gynecology Wayne State University School of MedicineDetroitMichiganUSA Mucosal Immunology Studies Team National Institute of Allergy and Infectious DiseasesNational Institutes of HealthBethesdaMarylandUSA Department of Hematology and Oncology The First Hospital of Jilin UniversityChangchunChina Division of Pediatric Hematology and Oncology Children’s Hospital of MichiganDetroitMichiganUSA.

Acute myeloid leukemia(AML)is a serious *** 5-year survival rates remain frustratingly low(65%for children and 26%for adults).Resistance to frontline chemotherapy(usually cytarabine)often develops;therefore a new treatment modality is ***-2 family proteins play an important role in balancing cell survival and *** antiapoptotic Bcl-2 family proteins have been found to be dysregulated in ***-199,a BH3 mimetic,was developed to target antiapoptotic protein *** ABT-199 has demonstrated promising results,resistance *** studies in AML show that ABT-199 alone decreases the association of proapoptotic protein Bim with Bcl-2,but this is compensated by increased association of Bim with prosurvival protein Mcl-1,stabilizing Mcl-1,resulting in resistance to *** this study,we investigated the antileukemic activity of the Mcl-1-selective inhibitor A-1210477 in combination with ABT-199 in AML *** found that A-1210477 synergistically induced apoptosis with ABT-199 in AML cell lines and primary patient *** synergistic induction of apoptosis was decreased upon Bak,Bax and Bim *** A-1210477 treatment alone also increased Mcl-1 protein levels,combination with ABT-199 reduced binding of Bim to *** results demonstrate that sequestration of Bim by Mcl-1,a mechanism of ABT-199 resistance,can be abrogated by combined treatment with the Mcl-1 inhibitor A-1201477.

关键词： chemotherapy alone treatment

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利用微波烧结垃圾焚烧灰的实验研究

环境污染与防治 2022年第7期44卷 862-867,872页

作者：张元馨卢前明王震潘艺行马超郑州职业技术学院现代管理系河南郑州450121 河南工程学院资源与安全工程学院河南郑州451191 新疆维吾尔自治区煤炭科学研究所新疆乌鲁木齐830091

为实现生活垃圾焚烧飞灰和医疗垃圾焚烧底灰在建筑材料领域的资源化利用,研究了微波辐照参数、原料配比、试样尺寸和助熔剂对两者的混合物(简称复合垃圾焚烧灰)烧结特性的影响。结果表明:随着生活垃圾焚烧飞灰掺量的增加,烧结产物的表... 详细信息

为实现生活垃圾焚烧飞灰和医疗垃圾焚烧底灰在建筑材料领域的资源化利用,研究了微波辐照参数、原料配比、试样尺寸和助熔剂对两者的混合物(简称复合垃圾焚烧灰)烧结特性的影响。结果表明:随着生活垃圾焚烧飞灰掺量的增加,烧结产物的表观密度及单粒强度不断减小;在微波功率和煅烧时间一定的条件下,减小试样尺寸有利于提高烧结核心温度及烧结率;随着微波功率及煅烧时间增加,试样核心温度、烧结率及单粒强度不断提高,当煅烧时间延长到一定程度,试样烧结率增幅减缓,继续煅烧不利于节约能耗。微波作用下,复合垃圾焚烧灰通过CaO-SiO_(2)-Al_(2)O_(3)体系熔融反应生成以钙铝黄长石、橄榄石和玻璃相为主的烧结产物,其结构密实,具有较高强度;助熔剂的掺入促使更多外层复合垃圾焚烧灰颗粒发生熔融,进而提高了试样烧结率。微波烧结后的复合垃圾焚烧灰中重金属稳定性得以提高,浸出量低于国家标准限值。

关键词：生活垃圾焚烧飞灰医疗垃圾焚烧底灰微波烧结烧结率助熔剂

基于降维变换的低复杂度双基地EMVS-MIMO雷达高分辨多维参数估计

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系统工程与电子技术 2024年第6期46卷 1899-1907页

作者：谢前朋杜奕航孙兵闫华潘小义赵锋中国人民解放军95913部队辽宁沈阳110041 国防科技大学第六十三研究所江苏南京210007 中国卫星海上测控部江苏江阴214430 国防科技大学电子信息系统复杂电磁环境效应国家重点实验室湖南长沙410073

针对当前算法在实现双基地电磁矢量传感器多输入多输出(electromagnetic vector sensors multiple input multiple output,EMVS-MIMO)雷达的多维参数估计时计算代价较高的问题,通过利用降维变换技术来实现低复杂度的角度参数和极化参数... 详细信息

针对当前算法在实现双基地电磁矢量传感器多输入多输出(electromagnetic vector sensors multiple input multiple output,EMVS-MIMO)雷达的多维参数估计时计算代价较高的问题,通过利用降维变换技术来实现低复杂度的角度参数和极化参数求解。针对阵列接收数据维度较大问题,通过设计相应的波束空间变换矩阵来实现对阵列接收数据的降维处理。针对算法本身的较高计算复杂度问题,采用低计算复杂度的平行因子分解算法。所提算法能够精确地实现对发射因子矩阵和接收因子矩阵的求解。同时,通过新的旋转不变关系构建新的估计信号参数,可以实现对发射/接收俯仰角的求解。进一步,发射/接收方位角、发射/接收极化角和发射/接收极化相位差的估计可以通过发射/接收空间响应矩阵的重构来实现。仿真实验结果表明,所提算法在降低计算复杂度的同时能够保持优越的多维参数估计性能。

关键词：双基地电磁矢量传感器多输入多输出(electromagnetic vector sensors multiple input multiple output,EMVS-MIMO)雷达多维参数估计波束空间变换平行因子分解算法

基于Timoshenko-Pasternak模型的盾构下穿既有隧道沉降简化计算方法

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城市轨道交通研究 2024年第10期27卷 21-29,38页

作者：王亮潘彬涛江杰龙逸航中铁一局集团第三工程有限公司宝鸡721006 广西大学土木建筑工程学院南宁530004 工程防灾与结构安全教育部重点实验室南宁530004

[目的]旨在解决在盾构下穿既有隧道沉降计算中,传统Euler-Bernoulli梁与Winkler地基模型精度不足问题。[方法]基于Timoshenko梁理论和Pasternak地基理论引入同时考虑土体剪切效应和隧道整体剪切变形的Timoshenko-Pasternak模型,通过推... 详细信息

[目的]旨在解决在盾构下穿既有隧道沉降计算中,传统Euler-Bernoulli梁与Winkler地基模型精度不足问题。[方法]基于Timoshenko梁理论和Pasternak地基理论引入同时考虑土体剪切效应和隧道整体剪切变形的Timoshenko-Pasternak模型,通过推导盾构下穿既有隧道的沉降控制方程,并使用有限差分法对其进行求解,得到沉降计算结果。之后将这些结果与有限元数值模拟解、实际工程监测数据及传统Euler-Bernoulli-Winkler模型的理论解进行详细对比。[结果及结论]基于Euler-Bernoulli-Winkler模型的计算结果在既有隧道沉降值上存在低估现象,而在既有隧道沉降槽宽度和管片张开量上存在高估现象,且无法计算既有隧道管片的错台量。相较之下,基于Timoshenko-Pasternak模型的计算结果与数值模拟及实际监测值更为一致。该模型能够较为准确地估计既有隧道沉降、沉降槽宽度及管片张开量,并提供了可靠的隧道管片的错台量计算结果。这对实际工程具有重要的指导意义,为实际工程提供了可靠的参考依据。

关键词：盾构下穿既有隧道沉降变形简化计算方法

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