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An octopamine-specific GRAB sensor reveals a monoamine relay circuitry that boosts aversive learning

National Science Review 2024年第05期 186-201页

作者： Mingyue Lv Ruyi Cai Renzimo Zhang Xiju Xia xuelin Li Yipan Wang Huan Wang Jianzhi Zeng yifei xue Lanqun Mao Yulong Li State Key Laboratory of Membrane Biology School of Life SciencesPeking University State Key Laboratory of Brain and Cognitive Science Institute of Biophysics Chinese Academy of Sciences PKU-IDG/Mc Govern Institute for Brain Research Yuanpei College Peking University Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary Studies Peking University Peking University–Tsinghua University–National Institute of Biological Sciences Joint Graduate Program Academy for Advanced Interdisciplinary Studies Peking University Institute of Molecular Physiology Shenzhen Bay Laboratory College of Chemistry Beijing Normal University Chinese Institute for Brain Research

Octopamine（OA）,analogous to norepinephrine in vertebrates,is an essential monoamine neurotransmitter in invertebrates that plays a significant role in various biological functions,including olfactory associative ***,the spatial and temporal dynamics of OA in vivo remain poorly understood due to limitations associated with the currently available methods used to detect *** overcome these limitations,we developed a genetically encoded GPCR activation-based（GRAB） OA sensor called *** sensor is highly selective for OA and exhibits a robust and rapid increase in fluorescence in response to extracellular *** GRABOA1.0,we monitored OA release in the Drosophila mushroom body（MB）,the fly's learning center,and found that OA is released in response to both odor and shock stimuli in an aversive learning *** OA release requires acetylcholine（ACh） released from Kenyon cells,signaling via nicotinic ACh ***,we discovered that OA amplifies aversive learning behavior by augmenting dopamine-mediated punishment signals via Octβ1R in dopaminergic neurons,leading to alterations in synaptic plasticity within the ***,our new GRABOA1.0sensor can be used to monitor OA release in real time under physiological conditions,providing valuable insights into the cellular and circuit mechanisms that underlie OA signaling.

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Drag increment induced by a small-scale forward-facing step in Mach number5 turbulent boundary layer flows

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Chinese Journal of Aeronautics 2020年第10期33卷 2491-2498页

作者： yifei xue Jie REN Jinling LUO Song FU School of Aerospace Engineering Tsinghua UniversityBeijing 100084China Department of Mechanical Engineering University of NottinghamNottingham NG72RDUnited Kingdom Beijing Aerospace Technology Institute Beijing 100074China

Small-scale roughness elements or imperfections are inevitable over the surface of a flight *** aerodynamics of these small-scale structures is difficult to predict but may play an important role in the design of a flight vehicle at high *** forward-facing step is a typical type of roughness *** experiments have been conducted to study the aerodynamics of supersonic forward-facing step,especially with a step height larger than boundary layer ***,few studies focus on small *** improve the understanding of small-scale forwardfacing step flow,we perform a series of simulations to analyze its aerodynamic influence on a Mach number 5 turbulent boundary *** general flow structures are analyzed and *** shock waves can be induced by the step even if the step height is much smaller than the boundary layer *** significant shocks are the separation shock and the reattachment *** influenced area by the step is *** the increase of the step height,the non-dimensional influence area decreases and gradually converges when the step height reaches the boundary layer *** are two normalized distributions of the skin friction coefficient and pressure coefficient associated with step *** using the normalized parameters,a power-law relationship between the step height and the drag increment coefficient is revealed and fits the simulation results *** is further illustrated that this relationship still holds when changing the inlet angle of attack,but needs slight modification with the angle of attack.

关键词： Aerodynamics Drag increment Forward-facing step Hypersonic flow Turbulent boundary layer

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Numerical simulations of sloshing and suppressing sloshing using the optimization technology method

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Applied Mathematics and Mechanics(English Edition) 2018年第6期39卷 845-854页

作者： Hui GUAN yifei xue Zhiiun WEI Chuijie WU College of Meteorology and Oceanography National University of Defense Technology Nanjing 211101 China School of Aerospace Engineering Tsinghua University Beijing 100084 China State Key Laboratory of Structural Analysis for Industrial Equipment School of Aeronautics and Astronautics Dalian University of Technology Dalian 116024 Liaoning Province China School of Aeronautics and Astronautics Dalian University of Technology Dalian 116024 Liaoning Province China

Sloshing is a common phenomenon in nature and industry, and it is important in many fields, such as marine engineering and aerospace engineering. To reduce the sloshing load on the side walls, the topology optimization and optimal control methods are used to design the shape of the board, which is fixed in the middle of the tank. The results show that the new board shape, which is designed via topology optimization, can significantly reduce the sloshing load on the side wall.

关键词： sloshing volume of fluid (VOF) method suppressing sloshing topologyoptimization

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基于光电传感的小动物主动运动行为评价及运动动机的神经机制研究

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中国体育科技 2024年第7期60卷 48-56页

作者：赵旭东薛亦飞王华林范益铭毛兰群侯莉娟北京师范大学北京100875

目的:构建小鼠主动运动行为量化评价系统,为运动与主动健康机制研究、慢病运动干预搭建平台,并通过在体实时采集主动运动不同阶段的脑电信号,探讨运动动机的神经调控机制。方法:1)构建基于光电传感的主动运动多模态系统(voluntary exerc... 详细信息

目的:构建小鼠主动运动行为量化评价系统,为运动与主动健康机制研究、慢病运动干预搭建平台,并通过在体实时采集主动运动不同阶段的脑电信号,探讨运动动机的神经调控机制。方法:1)构建基于光电传感的主动运动多模态系统(voluntary exercise multimodal system, VEMS),包括基于霍尔传感电信号的运动行为采集系统和基于机器视觉的运动轨迹分析系统;2)以C57BL/6小鼠为研究对象,应用VEMS系统记录小鼠主动运动行为并进行量化分析;3)结合在体多通道电生理记录技术采集小鼠主动运动不同阶段中脑腹侧被盖区(ventral tegmental area,VTA)神经元电活动,分析运动动机的神经编码特征。结果:1)VEMS系统可实现小鼠运动节律、运动距离、运动圈数、运动速度、运动时长和运动频次等行为数据的采集及量化分析;2)小鼠日运动时间主要集中在19:00—次日7:00,表现出较强的夜行节律,日运动距离最多可达15 km。每小时最大运动量出现在夜间1:00—2:00,运动圈数为0圈/10 min~170圈/10 min,每小时最大运动频次为23次,速度区间分布在0~12 m/min;3)在运动准备期、运动期及运动停止期,VTA神经元表现出不同的局部场单位(local field potentials,LFPs)特征信号,与运动停止期相比,运动准备期和运动期LFPs信号的θ频段(4~8 Hz)功率谱密度显著升高(P<0.001),且振荡显著增强(P<0.001);4)研究记录到59个对运动特异性响应的多巴胺(dopamine,DA)神经元,平均放电频率为(5.01±0.89) Hz,其中21%的DA神经元(Type-Ⅰ)在运动准备期高频放电,42%的DA神经元(Type-Ⅱ)在运动期有较强放电活动,37%(Type-Ⅲ)在运动停止后放电活动增多(P<0.001)。结论:1)本研究首次创建了VEMS系统,该系统能够对小鼠的主动运动行为进行多维度采集与量化评价分析,不仅为后期精准运动处方制定提供参考模型,还为运动神经科学的研究提供在体实时脑信号采集平台;2)小鼠主动运动行为受到VTA神经元特异性调节,DA神经元在运动准备期、运动期、运动停止期发挥不同作用。Type-Ⅰ和Type-Ⅲ神经元分别在运动准备期和运动停止期双向正反编码,即Type-Ⅰ神经元对奖励性正面信号表现出放电增强,而Type-Ⅲ神经元在运动停止时表现出特异放电。

关键词：主动运动运动动机光电传感在体多通道电生理 VTA神经元

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Merging non-covalent and covalent crosslinking:En route to single chain nanoparticles

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Chinese Chemical Letters 2024年第6期35卷 307-311页

作者： yifei Zhang Yuncong xue Laiwei Gao Rui Liao Feng Wang Fei Wang CAS Key Laboratory of Soft Matter Chemistry Department of Polymer Science and EngineeringUniversity of Science and Technology of ChinaHefei 230026China Department of Neurosurgical The First Affiliated Hospital of University of Science and Technology of ChinaDivision of Life Sciences and MedicineHefei 230026China

Single-chain nanoparticles represent an emerging class of nanomaterials designed to mimic protein's folding *** covalent crosslinking toward the formation of single-chain nanoparticles encounters complex energy landscapes,leading to the potential occurrence of misfolding *** noncovalent crosslinking can circumvent this issue,the resulting single-chain nanoparticles exhibit lower structural stability compared to their covalently crosslinked *** this study,we present a novel approach for the synthesis of single-chain nanoparticles,achieved through the combination of non-covalent and covalent intramolecular *** grafted onto the linear polymer form intrachain non-covalent stacks aided by hydrogen bonds,leading to the formation of non-covalently crosslinked single-chain *** nanoparticles undergo conversion to covalently crosslinked nanostructures through subsequent photo-irradiation using[2+2]photocycloaddition,a process facilitated by the supramolecular confinement ***,the resulting single-chain nanoparticles demonstrate both intrachain folding efficiency and substantial stability,offering significant potential for advancing applications across diverse fields.

关键词： Single chain nanoparticles Intrachain folding Supramolecular confinement Cyanostilbenes [2+2]photocycloaddition

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Whole-genome sequencing in medicinal plants:current progress and prospect

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Science China(Life Sciences) 2024年第2期67卷 258-273页

作者： yifei Pei Liang Leng Wei Sun Baocai Liu xue Feng Xiwen Li Shilin Chen Institute of Chinese Materia Medica China Academy of Chinese Medical SciencesBeijing 100700China Institute of Herbgenomics Chengdu University of Traditional Chinese MedicineChengdu 611137China Innovative Institute of Chinese Medicine and Pharmacy Chengdu University of Traditional Chinese MedicineChengdu 611137China Institute of Agricultural Bioresource Fujian Academy of Agricultural SciencesFuzhou 350003China

Advancements in genomics have dramatically accelerated the research on medicinal plants,and the development of herbgenomics has promoted the“Project of 1K Medicinal Plant Genome”to decipher their genetic ***,it is difficult to obtain their high-quality whole genomes because of the prevalence of polyploidy and/or high genomic *** genomes of 123 medicinal plants were published until September *** published genome sequences were investigated in this review,covering their classification,research teams,ploidy,medicinal functions,and sequencing *** than 1,000 institutes or universities around the world and 50 countries are conducting research on medicinal plant *** species account for a majority of sequenced medicinal *** whole genomes of plants in the Poaceae family are the most *** 40%of the published papers studied species with tonifying,replenishing,and heat-cleaning medicinal *** plants are still in the process of domestication as compared with crops,thereby resulting in unclear genetic backgrounds and the lack of pure lines,thus making their genomes more difficult to *** addition,there is still no clear routine framework for a medicinal plant to obtain a high-quality whole ***,a clear and complete strategy has been originally proposed for creating a high-quality whole genome of medicinal ***,whole genome-based biological studies of medicinal plants,including breeding and biosynthesis,were *** also advocate that a research platform of model medicinal plants should be established to promote the genomics research of medicinal plants.

关键词： herbgenomics whole genome high throughput sequencing research strategy model species application

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非适应性完美主义对青少年自伤行为的影响:一个有调节的链式中介模型

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教育生物学杂志 2024年第1期12卷 19-25页

作者：唐燊侯牧天薛亦菲牟韵静王宇拓宁维卫西南交通大学应用心理学研究院成都611756

目的探讨非适应性完美主义对自伤行为的影响,并分析认知融合和睡眠质量的中介作用和家庭功能的调节作用。方法采用中文Frost多维度完美主义问卷、青少年非自杀性自伤行为评定问卷、认知融合问卷、匹兹堡睡眠质量指数量表、家庭功能评... 详细信息

目的探讨非适应性完美主义对自伤行为的影响,并分析认知融合和睡眠质量的中介作用和家庭功能的调节作用。方法采用中文Frost多维度完美主义问卷、青少年非自杀性自伤行为评定问卷、认知融合问卷、匹兹堡睡眠质量指数量表、家庭功能评定量表总体功能分量表对843名中学生进行调查。结果非适应性完美主义、认知融合、睡眠质量、自伤行为和家庭功能,两两之间呈显著正相关。非适应性完美主义可以通过认知融合、睡眠质量的中介作用和认知融合—睡眠质量的链式中介作用对自伤行为产生间接影响。家庭功能调节模型中的两条路径,相对于家庭功能不良的个体,家庭功能良好个体的非适应性完美主义对自伤行为的直接作用更弱,其睡眠质量对自伤行为的影响较小。结论研究结果揭示了非适应性完美主义对青少年自伤行为的影响机制,以及心理灵活性、睡眠质量、家庭功能的保护性作用,对预防和干预青少年自伤行为具有一定的启示意义。

关键词：非适应性完美主义自伤行为认知融合睡眠质量家庭功能

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Achieving Negatively Charged Pt Single Atoms on Amorphous Ni(OH)_(2)Nanosheets with Promoted Hydrogen Absorption in Hydrogen Evolution

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Nano-Micro Letters 2024年第10期16卷 213-224页

作者： Yue Liu Gui Liu Xiangyu Chen Chuang xue Mingke Sun yifei Liu Jianxin Kang Xiujuan Sun Lin Guo School of Chemistry Beijing Advanced Innovation Center for Biomedical EngineeringKey Laboratory of Bio-inspired Smart Interfacial Science and TechnologyBeihang UniversityBeijing 100191People's Republic of China School of Chemistry Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of EducationXiangtan UniversityXiangtan 411105HunanPeople'Republic of China

Single-atom(SA)catalysts with nearly 100%atom utilization have been widely employed in electrolysis for decades,due to the outperforming catalytic activity and ***,most of the reported SA catalysts are fixed through the strong bonding between the dispersed single metallic atoms with nonmetallic atoms of the substrates,which greatly limits the controllable regulation of electrocatalytic activity of SA *** this work,Pt-Ni bonded Pt SA catalyst with adjustable electronic states was successfully constructed through a controllable electrochemical reduction on the coordination unsaturated amorphous Ni(OH)_(2)nanosheet *** on the X-ray absorption fine structure analysis and first-principles calculations,Pt SA was bonded with Ni sites of amorphous Ni(OH)_(2),rather than conventional O sites,resulting in negatively charged Pt^(δ-).In situ Raman spectroscopy revealed that the changed configuration and electronic states greatly enhanced absorbability for activated hydrogen atoms,which were the essential intermediate for alkaline hydrogen evolution *** hydrogen spillover process was revealed from amorphous Ni(OH)_(2)that effectively cleave the H-O-H bond of H_(2)O and produce H atom to the Pt SA sites,leading to a low overpotential of 48 mV in alkaline electrolyte at-1000 mA cm^(-2)mg^(-1)_(Pt),evidently better than commercial Pt/C *** work provided new strategy for the control-lable modulation of the local structure of SA catalysts and the systematic regulation of the electronic states.

关键词： Hydrogen evolution reaction Amorphous Pt single atoms Hydrogen spillover

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Molecular quantification of herbs(Herb-Q):a pyrosequencingbased approach and its application in Pinellia ternata

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Chinese Journal of Natural Medicines 2024年第7期22卷 663-672页

作者： PEI yifei LIU Ziyi YU Dade ZHANG Xiangyu SUN Wei CHEN Xiaofang FENG xue LI Xiwen Institute of Chinese Materia Medica China Academy of Chinese Medical SciencesBeijing 100700China Bijie Institute of Traditional Chinese Medicine Bijie 551700China The Key Laboratory for Health Industry of Bijie Bijie Medical CollegeBijie 551700China

Variations in herb dosage due to species adulteration and dosing inaccuracies can substantially affect clinical safety and *** species quantification remains challenging,as current methods often yield inconsistent *** study introduces a novel pyrosequencing-based technique,termed herb molecular quantification(Herb-Q),designed to precisely quantify herbal *** evaluated its effectiveness using Pinellia ternata and five of its ***,we assessed commonly used DNA barcodes with sequences from a public database,identifying two candidate regions,Maturase K(matK)and internal transcribed spacer 2(ITS2),for screening specific single nucleotide polymorphism(SNP)loci,allowing for species-specific *** loci were validated by amplifying and sequencing genomic material from collected *** validation studies showed that Herb-Q demonstrated excellent linearity,accuracy,repeatability,and detection *** established quantitative standard curves with high R^(2)values(>0.99)to enable precise species quantification,which were combined with external standards to provide clear and accurate visual quantification *** average bias in quantifying the tuber of *** was 2.38%,confirming that Herb-Q can accurately identify and quantify herbal product ***,the entire quantification process took less than 4 *** study presents a novel,rapid method for accurately quantifying species in herbal products and advances the application of DNA barcoding from species identification to quantitative detection.

关键词： Quantification Adulterant DNA barcodes Single nucleotide polymorphism

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Transplantation of human placental chorionic plate-derived mesenchymal stem cells for repair of neurological damage in neonatal hypoxic-ischemic encephalopathy

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Neural Regeneration Research 2024年第9期19卷 2027-2035页

作者： Lulu xue Ruolan Du Ning Bi Qiuxia Xiao yifei Sun Ruize Niu Yaxin Tan Li Chen Jia Liu Tinghua Wang Liulin Xiong Transformation Research Laboratory The Affiliated Hospital of Zunyi Medical UniversityZunyiGuizhou ProvinceChina State Key Laboratory of Biotherapy Sichuan UniversityChengduSichuan ProvinceChina Institute of Neurological Disease Translational Neuroscience CenterWest China HospitalSichuan UniversityChengduSichuan ProvinceChina Department of Animal Zoology Institute of NeuroscienceKunming Medical UniversityKunmingYunnan ProvinceChina Department of Anesthesiology The Affiliated Hospital of Zunyi Medical UniversityZunyiGuizhou ProvinceChina Department of Pediatrics the People’s Liberation Army Rocket Force Characteristic Medical CenterBeijingChina

Neonatal hypoxic-ischemic encephalopathy is often associated with permanent cerebral palsy,neurosensory impairments,and cognitive deficits,and there is no effective treatment for complications related to hypoxic-ischemic *** therapeutic potential of human placental chorionic plate-derived mesenchymal stem cells for various diseases has been ***,the potential use of human placental chorionic plate-derived mesenchymal stem cells for the treatment of neonatal hypoxic-ischemic encephalopathy has not yet been *** this study,we injected human placental chorionic plate-derived mesenchymal stem cells into the lateral ventricle of a neonatal hypoxic-ischemic encephalopathy rat model and observed significant improvements in both cognitive and motor *** chip analysis showed that interleukin-3 expression was significantly elevated in neonatal hypoxic-ischemic encephalopathy model *** transplantation of human placental chorionic plate-derived mesenchymal stem cells,interleukin-3 expression was *** further investigate the role of interleukin-3 in neonatal hypoxic-ischemic encephalopathy,we established an in vitro SH-SY5Y cell model of hypoxic-ischemic injury through oxygen-glucose deprivation and silenced interleukin-3 expression using small interfering *** found that the activity and proliferation of SH-SY5Y cells subjected to oxygen-glucose deprivation were further suppressed by interleukin-3 ***,interleukin-3 knockout exacerbated neuronal damage and cognitive and motor function impairment in rat models of hypoxic-ischemic *** findings suggest that transplantation of hpcMSCs ameliorated behavioral impairments in a rat model of hypoxic-ischemic encephalopathy,and this effect was mediated by interleukin-3-dependent neurological function.

关键词： behavioral evaluations gene knockout human neuroblastoma cells(SH-SY5Y) human placental chorionic derived mesenchymal stem cells interleukin-3 neonatal hypoxic-ischemic encephalopathy nerve injury oxygen-glucose deprivation protein chip small interfering RNA

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