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GSPT1 Functions as a Tumor Promoter in Human Liver Cancer

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Current Medical Science 2023年第1期43卷 104-114页

作者： yi-qing xi Jing-bo GAO Xuan-fei LI Li-hua XU Zhi-LI Li-jie YANG Jing WANG Hua-qiao WANG xiao-chang FANG Si-rui HUANG Wei xiE Mao-hui FENG Jing-wei ZHANG Department of Breast and Thyroid Surgery Zhongnan Hospital of Wuhan UniversityWuhan430071China Department of Gastrointestinal Surgery Zhongnan Hospital of Wuhan UniversityWuhan430071China Hubei Cancer Clinical Study Center Wuhan UniversityWuhan430071China Institute of Hydrobiology Chinese Academy of SciencesWuhan430071China

Objective This study analyzed the role of G1 to S phase transition 1 protein(GSPT1)in promoting progression of liver cancer *** A bioinformatics database was used to analyze the expression levels of GSPT1 in liver cancer tissues and the prognosis of ***,Western blotting and quantitative PCR were used to verify the expression levels of GSPT1 between normal hepatocytes and hepatoma *** used a CRISPR/Cas9 system to construct knockouts of GSPT1 in HepG2 and HCCLM9 liver cancer *** effect of GSPT1 on liver cancer cell migration and invasion was analyzed using flow cytometry,migration,and tumor formation *** The Cancer Genome Atlas Liver Hepatocellular Carcinoma dataset indicated that GSPT1 expression was upregulated in liver cancer cell lines,and patients with liver cancer had poor *** of GSPT1 in cells significantly inhibited tumor proliferation,cell migration,and growth in *** In this study,we found that GSPT1 promotes the migration and invasion of liver cancer cells.

关键词： Gl to S phase transition I protein liver cancer migration invasion

Expression of Stanniocalcin 2 in Breast Cancer and Its Clinical Sigmiicance

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Current Medical Science 2019年第6期39卷 978-983页

作者： Shu-ting JIANG Hua-qiao WANG Tie-cheng YANG Dan-wen WANG Li-jie YANG yi-qing xi Fan-zheng KONG Xue-kai PAN Li-hua XU Mao-hui FENG Wei xiE Fei SU Department of Breast Surgery Changzhou Maternity and Child Health Care Hospital Affiliated to Nanjing Medical UniversityChangzhou 213000China Department of Gastrointestinal Surgery Zhongtum Hospital of Wuhan UniversityWuhan 430071China Department of Thyroid and Breast Surgery Zhongnan Hospital of Wuhan UniversityClinical Cancer Study Center of Hubei ProvenceKey Laboratory of Tumor Biological Behavior of Hubei ProvenceWuhan 43007lt China Department of Medical Oncology No.I Hospital of Lanzhou UniversityLanzhou 730000China

This study aims to explore the expression of stanniocalcin 2(STC2)gene in breast cancer and its clinical *** patients with breast cancer from Zhongnan Hospital of Wuhan University admitted during March 2014 to October 2014 were enrolled in this *** the tissues used in this experiment included 50 cases of breast cancer tissues and corresponding 50 cases of paracancer normal breast tissues with complete patients'*** real-time quantitative polymerase chain reaction(qPCR)was applied to detect the expression of STC2 gene in 50 cases of breast cancer and paracancer normal breast *** results showed that the expression level of STC2 gene in 50 cases of breast cancer tissues was significantly higher than that in paracancer normal breast tissues(P0.05).In conclusion,the abnormal overexpression of STC2 gene may play a role in the development and progression of breast cancer,and it can be used as an independent metastasis and prognostic factor of breast *** addition,STC2 gene probably promotes the development and metastasis of breast cancer by interacting with estrogen and ER,and it may become a new direction for breast cancer endocrine therapy.

关键词： stanniocalcin 2 breast cancer real-time quantitative polymerase chain reaction metastasis

型温和退火对铸造Mg-12%Li-Al合金组织和性能的影响

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铸造 2007年第1期56卷 62-64页

作者：易庆喜黄长清陈振华刘兴湖南大学材料科学与工程学院湖南长沙410082

采用电阻炉熔炼、锂盐覆盖、氩气保护的工艺方法,在浇注温度为720℃,型温分别在100℃、200℃和300℃试验条件下,制备超轻Mg-12%Li-Al铸造镁合金并且均匀化退火。通过对显微组织和力学性能的分析,结果表明:超轻Mg-12%Li-Al铸造镁合金在... 详细信息

采用电阻炉熔炼、锂盐覆盖、氩气保护的工艺方法,在浇注温度为720℃,型温分别在100℃、200℃和300℃试验条件下,制备超轻Mg-12%Li-Al铸造镁合金并且均匀化退火。通过对显微组织和力学性能的分析,结果表明:超轻Mg-12%Li-Al铸造镁合金在型温为300℃浇注时,合金的铸态组织和性能比较好;合金经过450K×5h固溶后,组织十分均匀,力学性能良好。

关键词：超轻Mg-12%Li-Al铸造镁合金均匀化显微组织力学性能

Effect of Grinding Temperatures on the Surface Integrity of a Nickel-based Superalloy

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厦门大学学报（自然科学版） 2002年第S1期41卷 120-121页

作者： YU yi-qing 1, XU xi-peng 1, XU Hong-jun 2 (1. College of Mechanical Engineering and Automation, Huaqiao Univers ity, Quanzhou 362011, China 2. Nanjing University of Aeronautics and Astronau tics, Nanjing 210016, China)

An experimental study was carried out to investigat e the influence of temperatures on workpiece surface integrity in surface grinding of a cast nickel-based superalloy with alumina abrasive wheels. Temperatur e response at the wheel-workpiece interface was measured using a grindable foil /workpiece thermocouple. Specimens with different grinding temperatures were obt ained through changing grinding conditions including depth of cut, workpiece fee d speed, and coolant supply. Changes in surface roughness, residual stress, meta llographies, ground surface morphology, and micro hardness on the specimens were then analyzed. Bending fatigue tests were separately conducted at room temperat ure and 950oC in an effort to evaluate the influence of temperatures on the serv ice life of the ground specimens. A different burning color was found on the gro und workpiece surfaces when grinding temperatures are over a critical value. Alo ng with the emergence of burning color, roughness of the ground workpiece surfac e increased greatly compared with the surfaces without burning color, which was attributed to the plastically deformed coatings on the workpiece surface with el evated temperatures. Excepting the surface roughness, other items concerning the surface integrity of the ground workpiece were not affected by temperatures pro vided that grinding temperatures are not high enough to cause grinding cracks. B ased on the findings in this study, the grinding of the nickel-based superalloy can be divided into two stages in order to increase production efficiency, in which case the first stage is to reach an high material removal rate without concerning of the presence of burning color, whereas the second stage is to remo ve the plastically deformed coatings in order to decrease surface roughness.

关键词： temperature surface grinding superalloy surf ace integrity

CaF_2对Mg-12%Li-1%Al合金组织和性能的影响

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矿冶工程 2007年第2期27卷 81-83页

作者：易庆喜黄长清肖凡平陈振华湖南大学材料科学与工程学院湖南长沙410082

试验熔制了Mg-12%Li-1%Al合金,研究了添加少量的CaF2对合金组织和性能的影响。通过显微观察可知,被还原元素Ca对显微组织中的β相(bcc)有细化作用,Ca主要富集在晶界处,合金中的晶界网格为Ca和Al的富集物,为Al2Ca化合物。显微组织细化机... 详细信息

试验熔制了Mg-12%Li-1%Al合金,研究了添加少量的CaF2对合金组织和性能的影响。通过显微观察可知,被还原元素Ca对显微组织中的β相(bcc)有细化作用,Ca主要富集在晶界处,合金中的晶界网格为Ca和Al的富集物,为Al2Ca化合物。显微组织细化机理可以认为,由于Ca在晶界处的吸附作用极大的抑制了晶粒的长大而使其细化。室温下进行轧制板材拉伸测试,结果表明,置换出来的元素Ca可以提高合金的强度和塑性。

关键词： Ca Mg-12%Li-1%Al合金晶粒细化力学性能

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城市综合管廊装配率计算方法研究

工程建设与设计 2024年第15期 101-104页

作者：周晨伊西庆华昕设计集团有限公司江苏无锡214072

预制装配率是评价装配式建筑的重要指标之一,但已有装配式建筑装配率的计算方法对城市综合管廊并不适用。论文以综合管廊为研究对象,在分析比较已有的建筑装配率计算方法的基础上,提出适用于装配式综合管廊的装配率计算方法。

关键词：综合管廊装配率计算方法推广应用

同方期刊数据库博看期刊评论

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直柴加氢装置加工航煤的工业试验

河南化工 2005年第7期22卷 30-31页

作者：伊西青肖凤良中国石油化工股份有限公司洛阳分公司河南洛阳471012

随着原油性质不断变差,航煤质量控制难度加大,时常出现硫醇硫、银片腐蚀、颜色不合格等问题。为了找到最佳的航煤精制途径,解决航煤质量问题,利用直柴加氢精制装置进行了加工航煤的工业试验。试验证明,直柴加氢精制工艺可行。

关键词：航空煤油腐蚀加氢精制试验

LncRNA GATA3-AS1促进乳腺癌细胞的增殖迁移及其机制

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医学新知 2022年第2期32卷 127-137页

作者：江琪习一清魏蕾武汉大学基础医学院病理生理学教研室武汉430071 武汉大学中南医院甲乳外科武汉430071

目的长链非编码RNA(long non-coding RNA,LncRNA)与多种肿瘤的发生发展密切相关。本研究旨在探讨LncRNA GATA3-AS1在乳腺癌进展中的生物学作用及其机制。方法使用starBase等在线数据库预测GATA3-AS1的下游靶基因GATA3,采用qRT-PCR技... 详细信息

目的长链非编码RNA(long non-coding RNA,LncRNA)与多种肿瘤的发生发展密切相关。本研究旨在探讨LncRNA GATA3-AS1在乳腺癌进展中的生物学作用及其机制。方法使用starBase等在线数据库预测GATA3-AS1的下游靶基因GATA3,采用qRT-PCR技术检测正常乳腺细胞与乳腺癌细胞中LncRNA GATA3-AS1的表达水平,通过在线数据库预测其表达与乳腺癌不同临床病理特征的关系。利用质粒转染实验获得低表达GATA3-AS1的细胞株,使用Cell Counting Kit-8(CCK8)实验、划痕实验、Transwell实验评估GATA3-AS1在乳腺癌进展中的功能,并采用蛋白印迹实验验证GATA3-AS1和GATA3的表达相关性。结果 GATA3-AS1在乳腺癌细胞系MDA-MB-231和MCF-7中显著高表达,通过bc-GenExMiner数据库在线分析,GATA3-AS1在年龄> 51岁、人类表皮生长因子受体2阴性(HER2-)、雌激素受体阳性(ER+)、孕激素受体阳性(PR+)、激素受体A阳性型(luminal A)的乳腺癌患者中表达量更高,且GATA3-AS1的表达与乳腺癌的总生存期呈显著负相关。体外实验表明,沉默GATA3-AS1可显著降低乳腺癌细胞系MDA-MB-231和MCF-7的增殖迁移能力。此外,在MDA-MB-231和MCF-7细胞系中敲低GATA3-AS1后,GATA3蛋白水平显著升高,提示GATA3-AS1的促癌作用可能是通过其蛋白编码基因GATA3的表达来介导。结论 LncRNA GATA3-AS1可能通过调节GATA3的表达促进乳腺癌肿瘤细胞的增殖与迁移。

关键词：长链非编码RNA GATA3-AS1 乳腺癌增殖迁移调控机制

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黑龙江黑斑狗鱼线粒体控制区的遗传变异

生态科学 2009年第3期28卷 239-241页

作者：伊西庆底晓丹章群暨南大学水生生物研究中心广州510632

测定了黑龙江抚远江段和虎林江段黑斑狗鱼（Esox reicherti）线粒体控制区5’端的560bp序列，在12尾黑斑狗鱼序列中未检测到任何变异位点，即所有个体的序列均完全一致，共享同一种单倍型，群体内和群体间的核苷酸多样性均为0。黑龙江... 详细信息

测定了黑龙江抚远江段和虎林江段黑斑狗鱼（Esox reicherti）线粒体控制区5’端的560bp序列，在12尾黑斑狗鱼序列中未检测到任何变异位点，即所有个体的序列均完全一致，共享同一种单倍型，群体内和群体间的核苷酸多样性均为0。黑龙江黑斑狗鱼线粒体DNA如此贫乏的遗传多样性，一方面可能是黑龙江水系黑斑狗鱼在更新世冰期可能曾遭受过严重的瓶颈效应；另一方面则可能因目前是国内黑斑狗鱼种群数量锐减，种质资源急剧衰退，导致种群遗传多样性的丧失。建议广泛取样调查不同地理种群，并采用AFLP和微卫星等分子标记检测遗传多样性，以确定遗传变异丰富的黑斑狗鱼种群加以保护，促进黑龙江流域黑斑狗鱼资源的有效保护和可持续利用。

关键词：黑斑狗鱼线粒体控制区遗传变异黑龙江