GSPT1 Functions as a Tumor Promoter in Human Liver Cancer

作     者：Yi-qing XI Jing-bo GAO Xuan-fei LI Li-hua XU Zhi-LI Li-jie YANG Jing WANG Hua-qiao WANG Xiao-chang FANG Si-rui HUANG Wei XIE Mao-hui FENG Jing-wei ZHANG Yi-qing XI;Jing-bo GAO;Xuan-fei LI;Li-hua XU;Zhi-LI;Li-jie YANG;Jing WANG;Hua-qiao WANG;Xiao-chang FANG;Si-rui HUANG;Wei XIE;Mao-hui FENG;Jing-wei ZHANG

作者机构：Department of Breast and Thyroid SurgeryZhongnan Hospital of Wuhan UniversityWuhan430071China Department of Gastrointestinal SurgeryZhongnan Hospital of Wuhan UniversityWuhan430071China Hubei Cancer Clinical Study CenterWuhan UniversityWuhan430071China Institute of HydrobiologyChinese Academy of SciencesWuhan430071China 

出 版 物：《Current Medical Science》 (当代医学科学（英文）)

年 卷 期：2023年第43卷第1期

页      面：104-114页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by grants from the National Natural Science Foundation of China(No.81770283,No.82070302 and No.81902018) Clinical Medical Research Center of Peritoneal Cancer of Wuhan(No.2015060911020462) Natural Science Foundation of Hubei Province(No.2019CFB109) Technology and Innovation Seed Found,Zhongnan Hospital of Wuhan University(No.znpy2018004) 

主　　题：Gl to S phase transition I protein liver cancer migration invasion 

摘      要：Objective This study analyzed the role of G1 to S phase transition 1 protein(GSPT1)in promoting progression of liver cancer *** A bioinformatics database was used to analyze the expression levels of GSPT1 in liver cancer tissues and the prognosis of ***,Western blotting and quantitative PCR were used to verify the expression levels of GSPT1 between normal hepatocytes and hepatoma *** used a CRISPR/Cas9 system to construct knockouts of GSPT1 in HepG2 and HCCLM9 liver cancer *** effect of GSPT1 on liver cancer cell migration and invasion was analyzed using flow cytometry,migration,and tumor formation *** The Cancer Genome Atlas Liver Hepatocellular Carcinoma dataset indicated that GSPT1 expression was upregulated in liver cancer cell lines,and patients with liver cancer had poor *** of GSPT1 in cells significantly inhibited tumor proliferation,cell migration,and growth in *** In this study,we found that GSPT1 promotes the migration and invasion of liver cancer cells.