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不同基因型牛病毒性腹泻病毒对BALB/c小鼠致病性研究

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畜牧与兽医 2024年第4期56卷 72-77页

作者：刘镝钺程子龙陈益陈思宇李文良毛立杨蕾蕾孙敏张纹纹熊富强刘茂军西藏农牧学院动物科学学院西藏林芝860000 江苏省农业科学院兽医研究所/农业农村部兽用生物制品工程技术重点实验室江苏南京210014 南京农业大学动物医学院江苏南京210095 兽用生物制品(泰州)国泰技术创新中心江苏泰州225300

牛病毒性腹泻病毒(BVDV)是危害养牛业的重要病原。2021年本实验室从患有肺炎的牛肺中分离出1株BVDV(毒株编号为JS2201),经鉴定为1b亚型。为了评价该分离株的毒力与致病性,分别建立了分离株与参考株BVDV-1型Oregon C24V株、BVDV-2型C1602... 详细信息

牛病毒性腹泻病毒(BVDV)是危害养牛业的重要病原。2021年本实验室从患有肺炎的牛肺中分离出1株BVDV(毒株编号为JS2201),经鉴定为1b亚型。为了评价该分离株的毒力与致病性,分别建立了分离株与参考株BVDV-1型Oregon C24V株、BVDV-2型C1602株BVDV感染BALB/c小鼠模型,通过检测攻毒后小鼠临床症状、体重变化、病毒血症情况、血液中白细胞与淋巴细胞的变化、组织器官的病理组织学变化及病毒载量,比较了分离株JS2201与1型和2型参考株的致病性差异。结果显示:BVDV分离株JS2201与参考株均能够成功感染BALB/c小鼠,攻毒BALB/c小鼠均形成病毒血症,导致血液中白细胞数量、淋巴细胞数量以及淋巴细胞百分比短暂下降;BVDV分离株JS2201株攻毒组小鼠肝脏、肾脏、肺脏、脾脏中BVDV载量要高于C24V、C1602株攻毒组;攻毒组小鼠均表现为间质性肺炎病变,JS2201株攻毒组与1型参考株C24V株攻毒组小鼠肺脏病理组织学变化相似,BVDV-2型参考株C1602株攻毒组小鼠肺脏病变更为严重。综上,本研究成功建立了不同亚型BVDV急性感染BALB/c小鼠的模型,且比较分析了分离株与参考株之间的致病性差异,为BVDV疫苗的研制以及致病机制的研究奠定了基础。

关键词：牛病毒性腹泻病毒 BALB/c小鼠致病性基因型

Machine learning algorithm to construct cuproptosis-and immunerelated prognosis prediction model for colon cancer

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World Journal of Gastrointestinal Oncology 2023年第3期15卷 372-388页

作者： Yuan-yi Huang Ting-Yu Bao Xu-Qi Huang Qi-Wen Lan Ze-min Huang Yu-Han Chen Zhi-De Hu Xu-Guang Guo Department of Clinical Laboratory Medicine The Third Affiliated Hospital of Guangzhou Medical UniversityGuangzhou 510150Guangdong ProvinceChina Department of Clinical Medicine The First Clinical School of Guangzhou Medical UniversityGuangzhou 511436Guangdong ProvinceChina Department of Clinical Medicine The Third Clinical S Department of Clinical Medicine The Sixth Clinical School of Guangzhou Medical UniversityGuangzhou 511436Guangdong ProvinceChina Department of Medical Imageology The Second Clinical School of Guangzhou Medical UniversityGuangzhou 511436Guangdong ProvinceChina Department of Laboratory Medicine The Affiliated Hospital of Inner Mongolia Medical UniversityHohhot 010010Inner Mongolia Autonomous RegionChina Guangdong Provincial Key Laboratory of Major Obstetric Diseases The Third Affiliated Hospital of Guangzhou Medical UniversityGuangzhou 510150Guangdong ProvinceChina Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes The Third Affiliated Hospital of Guangzhou Medical UniversityGuangzhou 510150Guangdong ProvinceChina Guangzhou Key Laboratory for Clinical Rapid Diagnosis and Early Warning of Infectious Diseases King Med School of Laboratory MedicineGuangzhou Medical UniversityGuangzhou 511436Guangdong ProvinceChina

BACKGROUND Over the past few years,research into the pathogenesis of colon cancer has progressed rapidly,and cuproptosis is an emerging mode of cellular *** the relationship between colon cancer and cuproptosis benefits in identifying novel biomarkers and even improving the outcome of the *** To look at the prognostic relationship between colon cancer and the genes associated with cuproptosis and the immune system in *** main purpose was to assess whether reasonable induction of these biomarkers reduces mortality among patients with colon *** Data obtained from The Cancer Genome Atlas and Gene Expression Omnibus and the Genotype-Tissue Expression were used in differential analysis to explore differential expression genes associated with cuproptosis and immune *** least absolute shrinkage and selection operator and Cox regression algorithm was applied to build a cuproptosis-and immune-related combination model,and the model was utilized for principal component analysis and survival analysis to observe the survival and prognosis of the patients.A series of statistically meaningful transcriptional analysis results demonstrated an intrinsic relationship between cuproptosis and the micro-environment of colon *** Once prognostic characteristics were obtained,the CDKN2A and DLAT genes related to cuproptosis were strongly linked to colon cancer:The first was a risk factor,whereas the second was a protective *** finding of the validation analysis showed that the comprehensive model associated with cuproptosis and immunity was statistically *** the component expressions,the expressions of HSPA1A,CDKN2A,and UCN3 differed *** analysis primarily reflects the differential activation of related immune cells and ***,genes linked to immune checkpoint inhibitors were expressed differently between the subgroups,which may reveal the mechanism of worse prognosis an

关键词： Cuproptosis Immune Colon cancer Prognosis models Immune infiltration analysis

Targeted bile acids and gut microbiome profiles reveal the hepatoprotective effect of WZ tablet(Schisandra sphenanthera extract) against LCA-induced cholestasis

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Chinese Journal of Natural Medicines 2020年第3期18卷 211-218页

作者： LI Dong-Shun HUANG Quan-Fei GUAN Li-Huan ZHANG Hui-Zhen LI Xi FU Kai-Li CHEN yi-Xin WAN Jian-Bo HUANG min BI Hui-Chang Guangdong Provincial Key Laboratory of New Drug Design and Evaluation School of Pharmaceutical SciencesSun Yat-Sen UniversityGuangzhou 510000China State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoMacao 999078China

Cholestasis is caused by the obstacle of bile formation or secretion and can develop into severe liver diseases. We previously reported the ethanol extract of Schisandra sphenanthera(Wuzhi tablet, WZ) can significantly protect against lithocholic acid(LCA)-induced intrahepatic cholestasis in mice, partially due to the activation of PXR pathway and promotion of liver ***, the effect of WZ on the bile acids profile and gut microbiome in cholestastic mice remain unknown. In this study, the effect of WZ against LCA-induced liver injury was evaluated and its effect on the bile acids metabolome and gut microbiome profiles in cholestastic mice was further investigated. Targeted metabolomics analysis was performed to examine the change of bile acids in the serum, liver, intestine and feces. The change of intestinal flora were detected by the genomics method. Targeted metabolomics analysis revealed that WZ enhanced the excretion of bile acids from serum and liver to intestine and feces. Genomics analysis of gut microbiome showed that WZ can reverse LCA-induced gut microbiome disorder to the normal level. In conclusion, WZ protects against LCAinduced cholestastic liver injury by reversing abnormal bile acids profiles and alteration of gut microbiome.

关键词： Cholestasis Wuzhi tablet Schisandra sphenanthera Metabolome Gut microbiome Bile acids

Effects of endothelial nitric oxide synthase uncoupling on pulmonary endothelial dysfunction in rats with decompression sickness

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Military Medical Research 2015年第3期3卷 166-171页

作者： Hai-Shan Lin min Ou yi-Qun Fang Third Clinical Medical School in Southern Medical University Department of VIP Respiratory Navy General Hospital of PLA Navy Medical Research Unit

Background: To investigate the effects of unsafe decompression on rat pulmonary endothelial function and its relevant ***: Sixty male Sprague-Dawley(SD) rats were randomly divided into a control group(n=30) and a decompression sickness(DCS) group(n=30). The DCS model was established by placing the rats in the DCS group in a pressurized cabin where they were exposed to a 600 k Pa compressed air environment for 60 min, and the pressure was then reduced by 100 k Pa/min until it reached atmospheric pressure. After the surviving rats in the DCS group and the rats in the control group were anesthetized, their pulmonary arteries were stripped to test the in vitro pulmonary artery endothelium-dependent vasodilation capacity. Western blotting was used to measure the expression and dissociation of endothelial nitric oxide synthase(e NOS) in pulmonary artery tissues and all protein nitration levels in pulmonary artery tissues; reactive oxygen species(ROS) formation was measured via in vitro pulmonary artery superoxide anion probe dihydroethidium(DHE) ***: After experiencing unsafe decompression, 10 of the 30 rats in the DCS group died. The pulmonary artery endothelium-dependent vasodilation capacity in the surviving rats decreased significantly(P0.05), but the ratio of e NOS monomer/dimer in the DCS group was significantly higher than that in the control group(P<0.05). All protein tyrosine nitration levels in the pulmonary artery tissues of the DCS group were significantly higher than those of the control group(P<0.05). The results of DHE staining showed that the amount of ROS formation in the pulmonary arteries of the DCS group was significantly higher than that of the control group(P<0.05).Conclusion: Unsafe decompression during a simulated submarine escape process can lead to e NOS dimer uncoupling in the pulmonary artery endot

关键词： Decompression sickness Nitric oxide synthase type III Superoxide anion 3-nitrotyrosine

Proteomic and bioinformatic analyses of possible targetrelated proteins of gambogic acid in human breast carcinoma MDA-MB-231 cells

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Chinese Journal of Natural Medicines 2015年第1期13卷 41-51页

作者： LI Dong SONG Xiao-yi Yue Qing-Xi cui Ya-Jun LIU Miao FENG Li-Xing WU Wan-ying JIANG Bao-Hong YANG min QU Xiao-Bo LIU Xuan GUO De-An Changchun University of Chinese Medicine Shanghai Institute of Materia Medica Chinese Academy of Sciences Shanghai University of Traditional Chinese Medicine

Gambogic acid （GA） is an anticancer agent in phase IIb clinical trial in China but its mechanism of action has not been fully clarified. The present study was designed to search the possible target-related proteins of GA in cancer cells using proteomic method and establish possible network using bioinformatic analysis. Cytotoxicity and anti-migration effects of GA in MDA-MB-231 cells were checked using MTT assay, flow cytometry, wound migration assay, and chamber migration assay. Possible target-related proteins of GA at early （3 h） and late stage （24 h） of treatment were searched using a proteomic technology, two-dimensional electro- phoresis （2-DE）. The possible network of GA was established using bioinformatic analysis. The intracellular expression levels of vimentin, keratin 18, and calumenin were determined using Western blotting. GA inhibited cell proliferation and induced cell cycle arrest at G2/M phase and apoptosis in MDA-MB-231 cells. Additionally, GA exhibited anti-migration effects at non-toxic doses. In 2-DE analysis, totally 23 possible GA targeted proteins were found, including those with functions in cytoskeleton and transport, regulation of redox state, metabolism, ubiquitin-proteasome system, transcription and translation, protein transport and modification, and cytokine. Network analysis of these proteins suggested that cytoskeleton-related proteins might play important roles in the effects of GA. Results of Western blotting confirmed the cleavage of vimentin, increase in keratin 18, and decrease in calumenin levels in GA-treated cells. In summary, GA is a multi-target compound and its anti-cancer effects may be based on several target-related pro- teins such as cytoskeleton-related proteins.

关键词： Gambogic acid MDA-MB-231 cells Anti-cancer Proteomic Bioinfor matic

许德公人参黄精杏麦饮对小鼠免疫增强活性研究

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辽宁中医杂志 2024年第6期51卷 147-150,I0007-I0009页

作者：雷虹筠崔雪洁许震许先梅钱志刚荣梅芬卓茵许云涛魏星星李皖婧徐俊武毅杜茂波中国中医科学院中药研究所北京100700 南京农业大学动物医学院江苏南京210009 山东中医药大学药学院山东济南250355 芜湖市诺康生物科技有限公司安徽芜湖241000

目的探究许德公人参黄精杏麦饮对小鼠免疫功能的作用,阐明其免疫增强的作用机制。检测各浓度许德公人参黄精杏麦饮对脾脏淋巴细胞的安全浓度、脾淋巴细胞因子分泌水平变化、淋巴细胞亚群的变化。方法将40只BALB/c小鼠随机分为4组(n=10)... 详细信息

目的探究许德公人参黄精杏麦饮对小鼠免疫功能的作用,阐明其免疫增强的作用机制。检测各浓度许德公人参黄精杏麦饮对脾脏淋巴细胞的安全浓度、脾淋巴细胞因子分泌水平变化、淋巴细胞亚群的变化。方法将40只BALB/c小鼠随机分为4组(n=10),包括空白对照组,许德公人参黄精杏麦饮高、中、低剂量(1.2、0.6、0.3 mg/g)组,给药28 d后检测小鼠胸腺及脾脏指数,然后通过脾淋巴细胞转化实验、免疫器官组织学观察、小鼠血清免疫细胞因子水平变化、淋巴细胞亚群分布变化,评价许德公人参黄精杏麦饮的免疫调节作用。结果干预后的脾脏淋巴细胞的安全浓度最优为500μg/mL;淋巴细胞CD_(4)^(+)/CD_(8)^(+)的比例显著升高。此外,脾脏淋巴细胞分泌白细胞介素2(interleukin-2,IL-2)、白细胞介素6(interleukin-6,IL-6)、γ干扰素(interferon-γ,IFN-γ)及肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)水平均得到不同程度的上调。与空白对照组比较,高剂量许德公人参黄精杏麦饮能明显增加小鼠免疫器官脾脏指数(P<0.05);低、中、高剂量许德公人参黄精杏麦饮均能促进淋巴细胞增殖反应(P<0.05);小鼠脾脏白髓生发中心和边缘区淋巴细胞增生明显;能持续诱导脾脏淋巴细胞中CD_(4)^(+)和CD_(8)^(+)高表达。结论许德公人参黄精杏麦饮可以显著增强机体免疫力,其预防和治疗免疫功能失调相关疾病有较好的应用前景。

关键词：许德公人参黄精杏麦饮脾脏淋巴细胞免疫调节

Plant multiscale networks:charting plant connectivity by multi-level analysis and imaging techniques

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Science China(Life Sciences) 2021年第9期64卷 1392-1422页

作者： Xi Zhang yi Man Xiaohong Zhuang Jinbo Shen yi Zhang Yaning cui Meng Yu Jingjing Xing Guangchao Wang Na Lian Zijian Hu Lingyu Ma Weiwei Shen Shunyao Yang Huimin Xu Jiahui Bian Yanping Jing Xiaojuan Li Ruili Li Tonglin Mao Yuling Jiao Sodmergen Haiyun Ren Jinxing Lin Beijing Advanced Innovation Center for Tree Breeding by Molecular Design Beijing Forestry UniversityBeijing 100083China College of Biological Sciences and Biotechnology Beijing Forestry UniversityBeijing 100083China School of Life Sciences Centre for Cell&Developmental Biology and State Key Laboratory of AgrobiotechnologyThe Chinese University of Hong KongHong Kong 999077China State Key Laboratory of Subtropical Silviculture Zhejiang A&F UniversityHangzhou 311300China Key Laboratory of Cell Proliferation and Regulation Biology Ministry of EducationCollege of Life ScienceBeijing Normal UniversityBeijing 100875China Key Laboratory of Plant Stress Biology School of Life SciencesHenan UniversityKaifeng 457004China College of Biological Sciences China Agricultural UniversityBeijing 100193China State Key Laboratory of Plant Physiology and Biochemistry Department of Plant SciencesCollege of Biological SciencesChina Agricultural UniversityBeijing 100193China State Key Laboratory of Plant Genomics Institute of Genetics and Developmental BiologyChinese Academy of Sciencesand National Center for Plant Gene ResearchBeijing 100101China Key Laboratory of ministry of Education for Cell Proliferation and Differentiation College of Life SciencesPeking UniversityBeijing 100871China

In multicellular and even single-celled organisms,individual components are interconnected at multiscale levels to produce enormously complex biological networks that help these systems maintain homeostasis for development and environmental *** biology studies initially adopted network analysis to explore how relationships between individual components give rise to complex biological *** analysis has been applied to dissect the complex connectivity of mammalian brains across different scales in time and space in The Human Brain *** plant science,network analysis has similarly been applied to study the connectivity of plant components at the molecular,subcellular,cellular,organic,and organism *** of these multiscale networks contributes to our understanding of how genotype determines *** this review,we summarized the theoretical framework of plant multiscale networks and introduced studies investigating plant networks by various experimental and computational *** next discussed the currently available analytic methodologies and multi-level imaging techniques used to map multiscale networks in ***,we highlighted some of the technical challenges and key questions remaining to be addressed in this emerging field.

关键词： multiscale network connectivity cytoskeleton membrane contact site organelle interaction multicellularity connectome cytoarchitecture topological analysis multi-level imaging techniques

FpPDE1 function of Fsarium pseudograminearum on pathogenesis in wheat

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Journal of Integrative Agriculture 2017年第11期16卷 2504-2512页

作者： WANG Li-min ZHANG yi-fan DU Zhen-lin KANG Rui-jiao CHEN Lin-lin XING Xiao-ping YUAN Hong-xia DING Sheng-li LI Hong-lian Henan Agricultural University/Collaborative Innovation Center of Henan Grain Crops~State Key Laboratory of Wheat and Maize bythe Joint Construction of ministry of Science and Technology and Henan Province Zhengzhou 450002 P R. China

Wheat crown rot caused by Fusarium spp. is a common disease worldwide. Both Fusarium pseudograminearum and Fusarium graminearum infect wheat crown and produce mycotoxin leading to grain loss due to white head. F. pseudograminearum （Fp） was reported in wheat from Henan Province of China a couple of years ago. The wheat crown rot （CR） caused by this new pathogen is as an emerging severe disease of wheat, which has recently expanded to several provinces in China and is, therefore, under rapid investigation. Colonization of wheat tissue by Fp is accomplished though the formation of a septated foot-shaped appressoria and generation of a penetration peg to break through the internal cells of leaf sheath. The molecular mechanism by which Fp regulates the pathogenesis on wheat host is unclear. Here, we report FpPDE1, a P-type ATPase-encoding predicted PDE1 orthologue gene of Magnaporthe oryzae, belonging to the DRS2 subfamily of aminophospholipid translocases. The gene deletion of FpPDE1 with the split-marker approach did not obviously affect hyphae growth and conidiation, but led to an attenuated virulence on wheat base stem and root. Our finding indicates that the putative aminophospholipid translocases is not essential for the infectious hyphae development in Fp.

关键词： Fusarium pseudograminearum gene knockout FgPDE1 pathogenicity

Silybin alleviates hepatic lipid accumulation in methionine-choline deficient diet-induced nonalcoholic fatty liver disease in mice via peroxisome proliferator-activated receptorα

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Chinese Journal of Natural Medicines 2021年第6期19卷 401-411页

作者： cui Shuang PAN Xiao-Jie GE Chao-Liang GUO yi-Tong ZHANG Peng-Fei YAN Ting-Ting ZHOU Ji-Yu HE Qing-Xian CHENG Long-Hao WANG Guang-Ji HAO Hai-Ping WANG Hong State Key Laboratory of Natural Medicines Key Laboratory of Drug Metabolism&PharmacokineticsChina Pharmaceutical UniversityNanjing 210009China Department of Pharmacy The First Affiliated Hospital of Anhui Medical UniversityHefei 230022China Laboratory of Metabolism Center for Cancer ResearchNational Cancer InstituteNational Institutes of HealthBethesdaMD 20892USA

Nonalcoholic fatty liver disease(NAFLD)is regarded as the most common liver disease with no approved therapeutic drug ***,an extract from the seeds of Silybum marianum,has been used for centuries for the treatment of various liver *** the hepatoprotective effect of silybin against NAFLD is widely accepted,the underlying mechanism and therapeutic target remain *** this study,NAFLD mice caused by methionine-choline deficient(MCD)diet were orally administrated with silybin to explore the possible mechanism and *** clarify the contribution of peroxisome proliferator-activated receptorα(PPARα),PPARαantagonist GW6471 was co-administrated with silybin to NAFLD *** silybin was proven as a PPARαpartial agonist,the combined effect of silybin with PPARαagonist,fenofibrate,was then evaluated in NAFLD *** and liver samples were collected to analyze the pharmacological efficacy and expression of PPARαand its *** expected,silybin significantly protected mice from MCD-induced ***,silybin reduced lipid accumulation via activating PPARα,inducing the expression of liver cytosolic fatty acid-binding protein,carnitine palmitoyltransferase(Cpt)-1a,Cpt-2,medium chain acyl-CoA dehydrogenase and stearoyl-CoA desaturase-1,and suppressing fatty acid synthase and acetyl-CoA carboxylaseα.GW6471 abolished the effect of silybin on PPARαsignal and hepatoprotective effect against ***,as a partial agonist for PPARα,silybin impaired the powerful lipid-lowering effect of fenofibrate when used *** together,silybin protected mice against NAFLD via activating PPARαto diminish lipid accumulation and it is not suggested to simultaneously take silybin and classical PPARαagonists for NAFLD therapy.

关键词： Silybin NAFLD PPARa Lipid metabolism Fenofibrate