FpPDE1 function of Fsarium pseudograminearum on pathogenesis in wheat

作     者：WANG Li-min ZHANG Yi-fan DU Zhen-lin KANG Rui-jiao CHEN Lin-lin XING Xiao-ping YUAN Hong-xia DING Sheng-li LI Hong-lian 

作者机构：Henan Agricultural University/Collaborative Innovation Center of Henan Grain Crops~State Key Laboratory of Wheat and Maize bythe Joint Construction of Ministry of Science and Technology and Henan Province Zhengzhou 450002 P R. China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2017年第16卷第11期

页      面：2504-2512页

核心收录：

学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治] 

基　　金：financially supported by the National Special Fund for Agro-scientific Research in the Public Interest of China(201503112) the Basic and Advance Technology Research Program in Henan Province,China(152300410073) the Talent Project of Henan Agricultural University,China(3600861) 

主　　题：Fusarium pseudograminearum gene knockout FgPDE1 pathogenicity 

摘      要：Wheat crown rot caused by Fusarium spp. is a common disease worldwide. Both Fusarium pseudograminearum and Fusarium graminearum infect wheat crown and produce mycotoxin leading to grain loss due to white head. F. pseudograminearum （Fp） was reported in wheat from Henan Province of China a couple of years ago. The wheat crown rot （CR） caused by this new pathogen is as an emerging severe disease of wheat, which has recently expanded to several provinces in China and is, therefore, under rapid investigation. Colonization of wheat tissue by Fp is accomplished though the formation of a septated foot-shaped appressoria and generation of a penetration peg to break through the internal cells of leaf sheath. The molecular mechanism by which Fp regulates the pathogenesis on wheat host is unclear. Here, we report FpPDE1, a P-type ATPase-encoding predicted PDE1 orthologue gene of Magnaporthe oryzae, belonging to the DRS2 subfamily of aminophospholipid translocases. The gene deletion of FpPDE1 with the split-marker approach did not obviously affect hyphae growth and conidiation, but led to an attenuated virulence on wheat base stem and root. Our finding indicates that the putative aminophospholipid translocases is not essential for the infectious hyphae development in Fp.