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Circumnuclear Star Forming Activity in NGC 3982

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Chinese Journal of Astronomy and Astrophysics 2008年第5期8卷 555-565页

作者： Shui-Nai Zhang Qiu-Sheng Gu yi-peng wang Department of Astronomy Nanjing University Nanjing 210093 China

We present a study of the nearby Seyfert galaxy NGC 3982 using optical, infrared and X-ray data acquired by SDSS, Spitzer and Chandra. Our main results are as follows：（1） A simple stellar population synthesis on the nuclear and circumnuclear SDSS spectra gives unambiguous evidence of young stellar components in both the nuclear and circumnuclear regions. （2） The Spitzer Infrared Spectrograph （IRS） spectrum of the central region （∽ 3＂） shows a power-law continuum, a silicate emission feature at 9.7μm, and significant PAH emission features at 7.7, 8.6, 11.3 and 12.7 pro, suggesting the coexistence of AGN and starburst activities in the central region of NGC 3982. （3） We estimate the star formation rate （SFR） of the circumnuclear （∽ 5＂ - 20＂） region from the Hα luminosity to be SFRHα = 0.52 M⊙ yr^-1, which is consistent with the result from the Spitzer IRAC 8 μm luminosity, SFR8.0μm = 0.57 M⊙ yr^-1. （4） We measure the spectral energy distribution for the active nucleus of NGC 3982 from radio to X-ray, and obtain a bolometric luminosity of Lbol 4.5 × 10^42 erg s-1, corresponding to an Eddington ratio （Lbol//LEdd） of 0.014 The HST image of NGC 3982 shows a nuclear mini-spiral between the circumnuclear starforming region and the nucleus, which could be the channel through which gas is transported to the supermassive black hole from the circumnuclear star-forming region.

关键词： galaxies Seyfert - galaxies starburst - galaxies nuclei - galaxies individual (NGC 3982)

Development of re-crosslinkable dispersed particle gels for conformance improvement in extremely high-temperature reservoirs

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Petroleum Science 2022年第6期19卷 2922-2931页

作者： Dao-yi Zhu Run-Tian Luo Yang Liu Jun-Hui Qin Qi Zhao Hong-Jun Zhang Wan-Sheng wang Zi-Yuan wang Meng-En Zhu yi-peng wang peng-Bo Li China University of Petroleum-Beijing at Karamay Karamay 834000XinjiangChina

Micro-scale and nano-scale dispersed gel particles(DPG)are capable of deep migration in oil reservoirs due to their deformability,viscoelasticity,and suitable particle ***,it has been widely studied and applied in reservoir conformance control in recent ***,for highly permeable channels,their plugging performance is still *** addition,conventional in situ cross-linked polymer gels(ISCPGs)have fast gelation time under extremely high-temperature conditions,which often causes problems such as difficulty in ***,a re-cross linkable dispersed particle gel(RDPG)system applied for conformance control in highly permeable channels of extremely high-temperature petroleum reservoirs was *** particle size distribution,gelation time,gel strength,injection performance,and perfo rmance strength in po rous media were investigated using a laser particle size meter,the Sydansk bottle test method,rheometer,and core displacement experiments,*** show that the RDPG suspension can be stable for more than 6 months at room temperature with storage modulus G’much lower than 10 *** can pass through the pore throat by elastic deformation effect and does not cause strong ***,it can undergo re-crosslinking reaction at 150℃to form a strong bulk *** gel strength G’of re-crosslinked RDPG can be as high as 69.3 Pa,which meets the strength requirement of conformance *** RDPG suspension has the properties of easy injection,and it also has strong plugging,and high-temperature resistance after re-crosslinked in the core,which can be a very promising material for conformance improvement in extremely high-temperature reservoirs.

关键词： Dispersed particle gel Re-crosslinking Conformance improvement High-temperature petroleum reservoir Injection performance

4D label-free proteomic analysis of vitreous from patients with rhegmatogenous retinal detachment

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International Journal of Ophthalmology(English edition) 2023年第4期16卷 523-531页

作者： Qiu-yi Huo Meng-Chao Zhu Wen-Chao Yang yi-peng wang Song Chen Wenzhou Medical University Wenzhou 325027Zhejiang ProvinceChina Anyang Eye Hospital Anyang 455000Henan ProvinceChina Tianjin Eye Hospital Tianjin Eye InstituteTianjin Key Lab of Ophthalmology and Visual ScienceNankai University Affiliated Eye HospitalTianjin 300020China

AIM:To identify metabolites,proteins,and related pathways involved in the etiology of rhegmatogenous retinal detachment(RRD)for use as biomarkers in diagnosing and treating ***:Vitreous specimens were collected and liquid chromatography-tandem mass spectrometry analysis was per formed using the four-dimensional label-free *** significant differentially expressed proteins,gene ontology(GO)terms,Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway representations,and protein interactions were ***:Nine specimens were subjected to proteomic *** total,161 proteins were identified as differentially expressed proteins(DEPs),including 53 upregulated proteins and 108 downregulated *** functional analysis revealed that some DEPs were enriched in neuron-related terms and membrane protein ***,KEGG analysis indicated that the cell adhesion molecule metabolic pathway was associated with the greatest number of ***,the evaluation of protein-protein interaction network revealed that DEPs were clustered in neuronal adhesion,apoptosis,inflammation and immune responses,correct protein folding,and ***:Proteomic profiling is useful for the exploration of molecular mechanisms that underlie *** study reveals increased expression levels of proteins related to heat shock protein content,glycolysis,and inflammatory responses in *** regarding biomarkers of RRD pathogenesis may help to prevent the occurrence of RRD in the future.

关键词： vitreous rhegmatogenous retinal detachment proteome 4D label-free

Porous Alumina Ceramics Fabricated by Spark Plasma Sintering

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Chinese Journal of Aeronautics 2006年第B12期19卷 257-260页

作者： wang Kun FU Zheng-yi peng Yong wang Yu-cheng ZHANG Jin-yong ZHANG Qing-jie State Key Lab of Advanced Technology for Materials Synthesis and Processing Wuhan University of Technology Wuhan 430070 China

Porous alumina of regular spherical particles was fabricated with the spark plasma sintering （SPS） and then compared to those obtained through conventional hot pressing （HP）. The effects of the parameters of the heating process on porosity were also investigated, Microstructural studies suggest that porous ingots including regular pores be made out of regular spherical alumina particles due to the close sphere packages. A comparative study on the relative necks of the specimens produced by SPS and HP indicates an enhancement of neck growth with SPS. Contrasting the theoretical values to the experimental results over the relative necks indicates that a proper relationship between the relative necks and the porosity can be established by a sintering model.

关键词： spark plasma sintering (SPS) porous alumina ceramics sintering neck

Dynamic Expression Profiles of Marker Genes in Osteogenic Differentiation of Human Bone Marrow-derived Mesenchymal Stem Cells

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Chinese Medical Sciences Journal 2015年第2期30卷 108-113页

作者： Liang wang Zheng-yao Li yi-peng wang Zhi-hong Wu Bin Yu Department of Orthopedic Surgery Peking Union Medical College HospitalChinese Academy of Medical Sciences & Peking Union Medical College

Objective To observe the expression profiles of osteoblast-related genes in human mesenchymal stem cells(MSCs) derived from bone marrow during osteogenic differentiation. Methods MSCs were induced to differentiate with MSC osteogenic differentiation medium for 7, 14, 21 and 28 days respectively. Alizarin Red staining was used to detect matrix mineralization. Expression of osteoblast-related genes, including osteocalcin, osteopontin, Runt-related transcription factor 2(Runx2), alkaline phosphatase and collagen type Ⅰ, was assessed with quantitative reverse transcription-polymerase chain reaction. Results On day 14 after induction of differentiation, cells were stained positively with Alizarin Red. The expression levels of these genes exhibited an upward trend as induction time was prolonged. Exposure to osteogenic differentiation medium less than 21 days did not significantly induce osteocalcin expression; osteocalcin expression levels in the differentiated cells induced for 21 and 28 days were 1.63 and 2.46 times as high as the undifferentiated cells respectively(all P<0.05). Stimulation with MSC osteogenic differentiation medium over 14 days significantly enhanced bone marrow-derived MSCs to express osteopontin and Runx2 genes(all P<0.05). Osteogenic differentiation medium could significantly induce the expressions of alkaline phosphatase and collagen type Ⅰgenes(all P<0.05). Their expressions reached the peak levels on day 21, which were increased more than 4- and 3-fold respectively. Conclusion Human bone marrow-derived MSCs could exhibit the sequential expression pattern of osteoblast marker genes during osteogenic differentiation in vitro.

关键词： mesenchymal stem cell gene expression osteogenesis osteoblast differentiation

MANAGEMENT OF CEREBROSPINAL FLUID LEAKAGE FOLLOWING CERVICAL SPINE SURGERY

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Chinese Medical Sciences Journal 2008年第2期23卷 121-125页

作者： Ye Tian Ke-yi Yu yi-peng wang Jun Qian Gui-xing Qiu Department of Orthopaedics Peking Union Medical College Hospital Chinese Academy of Medical Sciences ＆Peking Union Medical College Beijing 100730

Objective To investigate the management and outcome of cerebrospinal fluid leakage(CSFL)after cervical *** Medical records of 642 patients who underwent cervical surgery between December 1999 and December 2005 at our hospital were retrospectively *** patients complicated by CSFL after surgery were enrolled,of which 4 cases were complicated after ossified posterior longitudinal ligament or posterior vertebral osteophyte resection directly injuring the dura,and 1 case after posterior cervical double-door laminoplasty without observed dural injury during *** the 5 CSFL cases,4 cases occurred at 1-3 days after operation and 1 case at 9 days after *** 5 postoperative CSFL cases were treated through wound drainage removal,wound sutures,prophylactic antibiotics,and continuous subarachnoid drainage in the elevated head *** All 5 CSFL cases experienced leakage cessation within 1-3 days and wound healing within 4-8 days,and subarachnoid drainage lasted 11-16 days with an average volume of 320 mL(range,150-410 mL).Four cases experienced headache,nausea and vomiting,1 case suffered from somnolence and hyponatremia,and symptoms subsided after symptomatic treatment and intravenous fluid *** patients were followed up for an average of 32 months(range,22-50 months).No occurrence of cerebrospinal fluid cyst or wound infection was *** produced no significant negative effects upon neuromuscular function *** Continuous subarachnoid cavity drainage in combination with elevated head position is a simple and safe non-surgical method in treatment of CSFL following cervical surgery.

关键词： cervical vertebra cerebrospinal fluid leakage subarachnoid drainage

Effects of C-terminal amidation and heptapeptide ring on the biological activities and advanced structure of amurin-9KY, a novel antimicrobial peptide identified from the brown frog, Rana kunyuensis

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Zoological Research 2019年第3期40卷 198-204页

作者： Fen Zhang Zhi-Lai Guo Yan Chen Li Li Hai-Ning Yu yi-peng wang Department of Pharmaceutical Sciences College of Pharmaceutical SciencesSoochow UniversitySuzhou Jiangsu 215123China Department of Bioscience and Biotechnology Dalian University of TechnologyDalian Liaoning 116023China School of Life Sciences Guizhou Normal UniversityGuiyang Guizhou 550001China

Rana kunyuensis is a species of brown frog that lives exclusively on Kunyu Mountain,Yantai,*** the current study,a 279-bp cDNA sequence encoding a novel antimicrobial peptide (AMP),designated as amurin-9KY,was cloned from synthesized double-strand skin cDNA of *** amurin-9KY precursor was composed of 62 amino acid (aa) residues,whereas the mature peptide was composed of 14 aa and contained two cysteines forming a C-terminal heptapeptide ring (Rana box domain) and an amidated *** structural characters represent a novel amphibian AMP *** amurin-9KY exhibited high similarity to the already identified amurin-9AM from ***,little is known about the structures and activities of amurin-9 family AMPs so ***,amurin-9KY and its three derivatives (amurin-9KY1-3) were designed and *** structures and activities were examined to evaluate the influence of C-terminal amidation and the heptapeptide ring on the activities and structure of amurin-9KY..Results indicated that C-terminal amidation was essential for antimicrobial activity,whereas both C-terminal amidation and the heptapeptide ring played roles in the low hemolytic *** dichroism (CD) spectra showed that the four peptides adopted an α-helical conformation in THF/H2O (v/v 1∶1) solution,but a random coil in aqueous *** of the C-terminal heptapeptide ring generated two free cysteine residues with unpaired thiol groups,which greatly increased the concentration-dependent anti-oxidant *** electron microscopy (SEM) was also performed to determine the possible bactericidal mechanisms.

关键词： Antimicrobial peptides Rana kunyuensis',Amurin-9KY Heptapeptide ring C-terminal amidation Structure activity relati on ship

Enhancing wheat regeneration and genetic transformation through overexpression of TaLAX1

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Plant Communications 2024年第5期5卷 9-24页

作者： Yang Yu Haixia Yu Jing peng wang Jinsong Yao yi peng wang Feng Li Zhang Shi Rong wang Yajie Zhao Xiang Yu Zhao Xian Sheng Zhang ying Hua Su State Key Laboratory of Crop Biology College of Life SciencesShandong Agricultural UniversityTai’anShandong 271018China

In the realm of genetically transformed crops,the process of plant regeneration holds utmost ***,the low regeneration efficiency of several wheat varieties currently restricts the use of genetic transformation for gene functional analysis and improved crop *** research explores overex-pression of TaLAX PANICLE1(TaLAX1),which markedly enhances regeneration efficiency,thereby boost-ing genetic transformation and genome editing in *** noteworthy is the substantial increase in regeneration efficiency of common wheat varieties previously regarded as recalcitrant to genetic *** study shows that increased expression of TaGROWTH-REGULATING FACTOR(TaGRF)genes,alongside that of their co-factor,TaGRF-INTERACTING FACTOR 1(TaGIF1),enhances cytokinin accumulation and auxin response,which may play pivotal roles in the improved regeneration and transfor-mation of TaLAX1-overexpressing wheat *** of TaLAX1 homologs also significantly in-creases the regeneration efficiency of maize and soybean,suggesting that both monocot and dicot crops can benefit from this *** shed light on a gene that enhances wheat genetic trans-formation and elucidate molecular mechanisms that potentially underlie wheat regeneration.

关键词： plant regeneration genetic transformation TaLAX1 TaGRF4–TaGIF1 cytokinin auxin wheat

Chronic photodamage in the chicken retina using 650-nm semiconductor laser

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International Journal of Ophthalmology(English edition) 2019年第6期12卷 936-942页

作者： yi-peng wang Ze-Yu Liang Song Chen Wen-Chao Yang Jia-Hui Kong Clinical College of Ophthalmology Tianjin Medical UniversityTianjin 300000China Anyang Eye Hospital Anyang 455000Henan ProvinceChina Tianjin Key Laboratory of Ophthalmology and Visual Science Tianjin Eye HospitalTianjin 300000China

AIM: To investigate the occurrence of chronic photodamage in the cone-based retina, following long-term exposure to a 650-nm semiconductor laser(power: 2 mW). METHODS: Chickens fed for 1 mo under natural light after hatching were irradiated with 650-nm laser light at different times each day. Fifteen animals were included in each group. Group A was a control group, irradiated with natural light during the entire study. Group B was irradiated with laser for 3 min/d. Group C was irradiated with laser for 6 min/d. Group D was irradiated with laser for 30 min/d. The duration of the light experiment was 6 mo. We obtained data at 1, 3, and 6 mo, including measuring the retinal thickness in vivo using optical coherence tomography, hematoxylin and eosin staining, TUNEL assay, apoptosis staining, malondialdehyde(MDA) content, superoxide dismutase(SOD) activity, and Western blotting to detect changes in L/M opsins and rhodopsin. RESULTS: At 1 mo, the MDA content in Group D was higher than that observed in Group A(P=0.019). At 3 mo the MDA content in Groups C and D was higher than that reported in Group A(P=0.026, 0.003). At 6 mo, the MDA content in Groups B, C, and D was higher than that observed in Group A(P=0.038, 0.032, 0.000, respectively). There was no difference in SOD activity, and L/M opsin and rhodopsin content between the groups at 1 and 3 mo. The SOD activity in group D was significantly decreased at 6 mo(P=0.000), as was the content of rhodopsin. There was no significant reduction observed in retinal thickness, abnormal cell arrangement, and positive staining of TUNEL in the groups during the 6-month study ***: Irradiation using a 650 nm semiconductor laser(power: 2 mW) for 6 min per day over 6 mo do not cause photodamage. Similarly, a 3-month exposure of 30 min per day do not cause damage. However, irradiation for 6 mo resulted in a significant increase in the content of free radicals and a decrease in the content of rhodopsin in the retina, suggest

关键词： light/adverse effects oxidative stress red light apoptosis chicken retina