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Pien Tze Huang Inhibits Proliferation of Colorectal Cancer Cells through Suppressing PNO1 Expression and Activating p53/p21 Signaling Pathway

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Chinese Journal of Integrative Medicine 2024年第6期30卷 515-524页

作者： CAO Liu-jing LIU Li-ya CHEN You-qin HAN Yu-ying WEI Li-hui YAO Meng-ying fang yi WU Mei-zhu CHENG ying Thomas J.Sferra LIU Hui-xin LI Li PENG Jun SHEN A-ling Clinical Research Institute the Second Affiliated Hospital&Academy of Integrative MedicineFujian University of Traditional Chinese MedicineFuzhou350122China Fujian Key Laboratory of Integrative Medicine in Geriatrics Fujian University of Traditional Chinese MedicineFuzhou350122China Department of Pediatrics Case Western Reserve University School of MedicineUH Rainbow Babies and Children’s HospitalClevelandOH44106USA Innovation and Transformation Center Fujian University of Traditional Chinese MedicineFuzhou350122China Department of Health Management Fujian Provincial HospitalShengli Clinical College of Fujian Medical UniversityFuzhou000000China

ObjectiveTo explore the regulatory effect of Pien Tze Huang (PZH) on targeting partner of NOB1 (PNO1) and it’s down-stream mediators in colorectal cancer (CRC) *** polymerase chain reaction was performed to determine mRNA levels of PNO1, TP53, and CDKN1A. Western blotting was performed to determine protein levels of PNO1, p53, and p21. HCT-8 cells were transduced with a lentivirus over-expressing PNO1. Colony formation assay was used to detect cell survival in PNO1 overexpression of HCT-8 cells after PZH treatment. Cell-cycle distribution, cell viability and cell apoptosis were performed to identify the effect of PNO1 overexpression on cell proliferation and apoptosis of HCT-8 cells after PZH treatment. Xenograft BALB/c nude mice bearing HCT116 cells transduced with sh-PNO1 or sh-Ctrl lentivirus were evaluated. Western blot assay was performed to detect PNO1, p53, p21 and PCNA expression in tumor sections. Terminal deoxynucleotidyl transferase dUTP nick end labling (TUNEL) assay was used to determine the apoptotic cells in *** treatment decreased cell viability, down-regulated PNO1 expression, and up-regulated p53 and p21 expressions in HCT-8 cells (P<0.05). PNO1 overexpression attenuated the effects of PZH treatment, including the expression of p53 and p21, cell growth, cell viability, cell cycle arrest and cell apoptosis in vitro (P<0.05). PNO1 knockdown eliminated the effects of PZH treatment on tumor growth, inhibiting cell proliferation inhibition and apoptosis induction in vivo (P<0.05). Similarly, PNO1 knockdown attenuated the effects of PZH treatment on the down-regulation of PNO1 and up-regulation of p53 and p21 in vivo (P<0.05).ConclusionThe mechanism by which PZH induces its CRC anti-proliferative effect is at least in part by regulating the expression of PNO1 and its downstream targets p53 and p21.

关键词： colorectal cancer Pien Tze Huang Chinese medicine partner of NOB1 proliferation p53/p21 pathway

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Plexin domain-containing 1 may be a biomarker of poor prognosis in hepatocellular carcinoma patients,may mediate immune evasion

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World Journal of Gastrointestinal Oncology 2024年第5期16卷 2091-2112页

作者： Ming-Yue Tang Xue Shen Run-Sheng Yuan Hui-Yuan Li Xin-Wei Li yi-Ming Jing Yue Zhang Hong-Hong Shen Zi-Shu Wang Lei Zhou Yun-Chuan Yang He-Xin Wen fang Su Department of Medical Oncology The First Affiliated Hospital of Bengbu Medical CollegeBengbu 233000Anhui ProvinceChina Otolaryngology and Head and Neck Surgery The First Affiliated Hospital of Bengbu Medical CollegeBengbu 233000Anhui ProvinceChina Department of Neurology The First Affiliated Hospital of Bengbu Medical CollegeBengbu 233000Anhui ProvinceChina Department of Hepatobiliary Surgery The First Affiliated Hospital of Bengbu Medical CollegeBengbu 233000Anhui ProvinceChina Department of Gastrointestinal Surgery The First Affiliated Hospital of Bengbu Medical CollegeBengbu 233000Anhui ProvinceChina

BACKGROUND For the first time,we investigated the oncological role of plexin domain-containing 1(PLXDC1),also known as tumor endothelial marker 7(TEM7),in hepatocellular carcinoma(HCC).AIM To investigate the oncological profile of PLXDC1 in *** Based on The Cancer Genome Atlas database,we analyzed the expression of PLXDC1 in *** immunohistochemistry,quantitative real-time polymerase chain reaction(qRT-PCR),and Western blotting,we validated our *** prognostic value of PLXDC1 in HCC was analyzed by assessing its correlation with clinicopathological features,such as patient survival,methylation level,tumor immune microenvironment features,and immune cell surface checkpoint ***,to assess the immune evasion potential of PLXDC1 in HCC,we used the tumor immune dysfunction and exclusion(TIDE)website and immunohistochemical staining *** Based on immunohistochemistry,qRT-PCR,and Western blot assays,overexpression of PLXDC1 in HCC was associated with poor *** and multivariate Cox analyses indicated that PLXDC1 might be an independent prognostic *** HCC patients with high methylation levels,the prognosis was worse than in patients with low methylation *** enrichment analysis of HCC tissues indicated that genes upregulated in the high-PLXDC1 subgroup were enriched in mesenchymal and immune activation signaling,and TIDE assessment showed that the risk of immune evasion was significantly higher in the high-PLXDC1 subgroup compared to the low-PLXDC1 *** high-risk group had a significantly lower immune evasion rate as well as a poor prognosis,and PLXDC1-related risk scores were also associated with a poor *** As a result of this study analyzing PLXDC1 from multiple biological perspectives,it was revealed that it is a biomarker of poor prognosis for HCC patients,and that it plays a role in determining immune evasion status.

关键词： Plexin domain-containing 1 Biomarker Immune evasion Prognosis Immunotherapy Hepatocellular carcinoma

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Prediction of hepatic artery occlusion after liver transplantation by ultrasound characteristics and clinical risk factors

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World Journal of Radiology 2024年第6期16卷 196-202页

作者： Yu-Ting Lai yi Chen Tai-Shi fang Zhi-Yan Li Ning-Bo Zhao Department of Ultrasound National Clinical Research Centre for Infectious DiseasesThe Third People’s Hospital of ShenzhenThe Second Hospital Affiliated with the Southern University of Science and TechnologyShenzhen 518112Guangdong ProvinceChina Department of Liver Surgery National Clinical Research Centre for Infectious DiseasesThe Third People’s Hospital of ShenzhenThe Second Hospital Affiliated with the Southern University of Science and TechnologyShenzhen 518112Guangdong ProvinceChina

BACKGROUND Hepatic artery occlusion(HAO)after liver transplantation(LT)is a devastating complication,resulting in early graft loss and reduced overall ***-sound is an established assessment method for HAO in patients following LT,especially those with complex hepatic artery *** We retrospectively analyzed the ultrasound characteristics and the clinic risk factors associated with HAO in 400 adult LT patients who were enrolled and treated at the Third People's Hospital of Shenzhen between November 2016 and July *** patients diagnosed with acute HAO(A-HAO)by surgery and fifteen diagnosed with chronic HAO(C-HAO)were included.A control group of 33 patients without HAO complications during the same period were randomly selected using a random number *** patients underwent an ultrasono-graphy *** including resistance index(RI),peak systolic velocity(PSV),and portal vein velocity(PVV)were compared across the ***,basic clinical data were collected for all patients,including gender,age,primary diagnosis,D-dimer concentration,total operation time,cold ischemia time,hot ischemia time,intraoperative blood loss and transfusion,intraoperative urine volume,infusion,model for end-stage liver disease(MELD)score,and whether complex hepatic artery reconstructions were ***,risk factors influencing HAO formation after LT were *** Compared to the non-HAO group,PVV and RI were higher in the A-HAO group,while PSV was ***,both PSV and RI were lower in the C-HAO group compared to the non-HAO *** proportion of patients undergoing complex hepatic artery reconstructions and the gamma-glutamyltransferase(GGT)level before occlusion were significantly higher in the A-HAO group compared to the non-HAO ***,there were no distinct differences between the two groups in D-dimer,MELD score,pre-occlusion alanine transaminase and aspartate transaminase levels,or intrao

关键词： Hepatic artery occlusion Ultrasonography Diagnostic performance Risk factors Liver transplantation

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考虑电池荷电状态的混合动力汽车复合电源协同控制

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吉林大学学报（工学版） 2024年第4期54卷 1114-1119页

作者：李房云夏容张怡欣南昌航空大学科技学院信息电子学部江西九江332020

针对复合电源的协同控制过程易受电池性能、电流效应、汽车速度等问题的影响,导致复合电源电流分配较不稳定的问题,提出了一种考虑电池荷电状态的混合动力汽车复合电源协同控制方法。该方法首先通过构建二阶电阻-电容(RC)等效电路模型... 详细信息

针对复合电源的协同控制过程易受电池性能、电流效应、汽车速度等问题的影响,导致复合电源电流分配较不稳定的问题,提出了一种考虑电池荷电状态的混合动力汽车复合电源协同控制方法。该方法首先通过构建二阶电阻-电容(RC)等效电路模型得到电池荷电状态的估算值;其次在估算值的基础上计算出复合电源的效率特性;最后采用自适应滤波器功率分配控制算法,通过构建目标函数和约束条件实现超级电容器与蓄电池的功率分担,进而完成混合动力汽车复合电源的协同控制。仿真结果表明,本文方法的电流分配情况较好,控制后的汽车能耗低,能耗始终控制在0.6~2.7 kW/h。

关键词：二阶RC等效电路模型放电效率参数优化电流补偿功率分配控制

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基于空间交叉卷积的轻量级人体姿态估计算法

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传感技术学报 2024年第3期37卷 439-445页

作者：方益石守东方靖森叶永芳蓝艇宁波大学信息科学与工程学院浙江宁波315211

针对改进轻量级OpenPose网络在预测阶段仍有较大参数量会降低模型推理速度,不利于在边缘设备部署的问题,提出一种基于改进卷积方法的人体姿态估计网络,使用空间交叉卷积来代替部分标准卷积,减少网络预测阶段的参数量。网络的输入为单目... 详细信息

针对改进轻量级OpenPose网络在预测阶段仍有较大参数量会降低模型推理速度,不利于在边缘设备部署的问题,提出一种基于改进卷积方法的人体姿态估计网络,使用空间交叉卷积来代替部分标准卷积,减少网络预测阶段的参数量。网络的输入为单目摄像头捕获的RGB图像,以MobileNetV3-Large为主干网络,并在其中加入了CBAM注意力模块,提取不同重要程度的空间和通道特征。获取图像特征后,送入两个分支中分别预测关键点位置和关键点组合关系。以空间交叉卷积代替两个分支中的部分标准卷积核,相对标准卷积能够减少80%的参数量。实验结果表明,相较于原方法,所提方法在精度下降较小的情况下,总参数量降低了22%,部署在CPU端的测试结果显示,速度能够达到6 FPS,提升了4倍。

关键词：人体姿态估计轻量级网络空间交叉卷积 OpenPose 边缘设备

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省以下分权式改革对城乡收入差距的影响研究——基于区县分化的视角

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财政研究 2024年第4期 85-100页

作者：杜彤伟方毅东北财经大学财政税务学院吉林大学数量经济研究中心

省以下的财政分权改革不仅是影响经济增长的关键因素,对缩小城乡差距也有重要作用。本文从区县分化的视角切入,以地市级为观察层面,采用地级市和市辖区两个维度的数据,研究以“省直管县”为代表的省以下财政分权改革是否缩小了区县的经... 详细信息

省以下的财政分权改革不仅是影响经济增长的关键因素,对缩小城乡差距也有重要作用。本文从区县分化的视角切入,以地市级为观察层面,采用地级市和市辖区两个维度的数据,研究以“省直管县”为代表的省以下财政分权改革是否缩小了区县的经济社会差距,进而降低了整体的城乡收入差距。研究发现,向县级政府分权降低了区、县在财力、教育、社保和经济方面的差距,最终通过显著增加农村居民的人均收入而缩小城乡收入差距。异质性分析表明,改革的效应在中部地区、南方地区和“强市-弱县”组表现得更明显。进一步的讨论发现,政府竞争、监督机制是影响分权改革效应的重要因素。

关键词：财政分权 “省直管县” 城乡收入差距区县分化

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基于联合方向梯度和均值对比度的红外弱小目标检测方法

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红外与毫米波学报 2024年第1期43卷 106-115页

作者：李宁郭义放焦继超逄敏徐威北京邮电大学电子工程学院北京100876 中国电波传播研究所第三研究部山东青岛266108

针对小目标在整幅图像中占比很低,且目标周围存在大量杂波,提出了一种基于联合方向梯度(Associated Directional Gradient,ADG)和均值对比度(Mean Contrast,MC)的红外弱小目标检测算法。该算法由两个模块组成:ADG利用红外弱小目标的高... 详细信息

针对小目标在整幅图像中占比很低,且目标周围存在大量杂波,提出了一种基于联合方向梯度(Associated Directional Gradient,ADG)和均值对比度(Mean Contrast,MC)的红外弱小目标检测算法。该算法由两个模块组成:ADG利用红外弱小目标的高斯分布模型,将单一方向的梯度与一个相邻方向上的梯度相加组成新的联合梯度特征,增强真实目标、抑制背景杂波的同时能够消除高亮边缘对目标检测效果的影响;MC融入方向信息来计算目标的多方向对比度,选用多方向对比度的最小值抑制结构噪声,并将均值滤波的思想引入对比度的计算,抑制背景中的孤立噪声,进一步降低检测的虚警率。在实际红外图像上的实验结果表明,该算法在增强目标信噪比和抑制背景噪声方面,能够取得较好效果。

关键词：红外图像目标检测联合方向梯度均值对比度

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数字基础设施政策赋能企业数字化转型:演进逻辑和政策取向

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求是学刊 2024年第2期51卷 59-68页

作者：汤蕴懿李方卓上海社会科学院应用经济研究所上海200000 上海社会科学院经济研究所上海200000

数字化转型是企业高质量发展的重要路径,发挥中国数字基础设施政策的战略性、基础性、先导性作用,厘清其在赋能企业数字化转型的演进过程和微观机理,并进一步明确政策优化调整方向,对激发数字经济发展动能的持续性具有重要意义。本文基... 详细信息

数字化转型是企业高质量发展的重要路径,发挥中国数字基础设施政策的战略性、基础性、先导性作用,厘清其在赋能企业数字化转型的演进过程和微观机理,并进一步明确政策优化调整方向,对激发数字经济发展动能的持续性具有重要意义。本文基于“供给侧改革”视角,采用文本分析方法总结各阶段数字基础设施政策的演进过程。研究发现:数字基础设施政策经历了硬件建设期、要素建设期和规制建设期三个阶段;数字基础设施政策在三个阶段分别通过提高企业生产效率、价值增值能力和集群的超额收益,持续深化企业数字化转型。微观机理方面,数字基础设施政策通过培育企业数字技术能力、提高要素配置效率,解决企业数字化面临的市场失灵问题。最后,本文从数据共享机制建设、多层次平台支撑和政策均衡投入三个方面提出未来政策取向。

关键词：数字基础设施政策企业数字化转型文本分析演进逻辑政策取向

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Sugar-Based Water Retention Agents in Meat Products: Enhancing Water-Holding Capacity and Promoting Health Benefits

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Food Science and Human Wellness 2024年

作者： Jiahui Ma Pengyu Lei Lei Wang Jia yi yilei Ma yimeng fang Linkai Qu Li Luo Kun Zhang Libo Jin Qinsi Yang Wei Wu Da Sun Bioengineering College of Chongqing University Institute of Life Sciences & Biomedical Collaborative Innovation Center of Zhejiang Province， Wenzhou University Wenzhou Institute， University of Chinese Academy of Sciences Affiliated Dongguan Hospital， Southern Medical University College of Veterinary Medicine， Jilin University

For the purpose of satisfying high demands for taste， color， flavor， and storage of meat products， water retention agents (WRAs) play an important role. Phosphate has been widely used as an attractive functional material for water retention in current practical applications. However， excessive phosphate addition and long-term consumption may be harmful impacts on health and the environment. Therefore， it is vital to develop safe and efficient phosphate-free WRAs for further improving water-holding capacity (WHC) efficacy and edible safety， especially in meat products. In particular， sugar water retention agents (SWRAs) are increasingly popular because of their perfect safety， excellent WHC， and superior biological properties. This review discusses the inducements and mechanisms underlying water loss in meat products. In addition， we focused on the research progresses and related mechanisms of SWRAs in the WHC of meat products and its unique biological functions， as well as the extraction technology. Finally， the future application and development of SWRA were prospected.

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Exploring the molecular mechanism of Suoquan pill in the treatment of diabetic kidney disease based on network pharmacology,molecular docking,in vitro experiment

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Traditional Medicine Research 2024年第11期9卷 27-37页

作者： Zi-Jie Yan Yu Kang Shu-Man Liu fang-Yu Wang Man Xiao yi-Qiang Xie College of Traditional Chinese Medicine Hainan Medical UniversityHaikou 571199China Graduate School Heilongjiang Academy of Traditional Chinese MedicineHarbin 150001China Department of Pharmacology College of PharmacyHainan Medical UniversityHaikou 571199China Key Laboratory of Biochemistry and Molecular Biology Hainan Medical UniversityHaikou 571199China.

Background:Diabetic kidney disease(DKD)is a microvascular complication of diabetes mellitus and is the main cause of end-stage renal *** pills(SQP)has a variety of pharmacological activities and multiple therapeutic effects,and it is used clinically as a basic formula for the treatment of ***:Public databases were used to identify SQP compounds and the potential targets of SQP and DKD.A drug-component-therapeutic target network was ***-protein interaction network analysis,Gene Ontology functional analysis,and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis were used to analyse the potential molecular mechanisms of SQP based on common targets of drugs and *** docking simulations were conducted to confirm the binding abity of the core compounds to key *** efficacy and predicted molecular mechanisms of SQP were validated using cell counting kit-8 assay,flow cytometry,and western blotting with HK-2 cells as a ***:Network pharmacology analysis showed that 26 compounds and 207 potential targets of SQP were involved in the treatment of DKD;boldine,denudatin B,pinocembrin,kaempferoid,and quercetin were considered core compounds,and epidermal growth factor receptor(EGFR)and proto-oncogene,non-receptor tyrosine kinase(SRC)were considered key *** Ontology enrichment analysis indicated that protein phosphorylation and negative regulation of apoptotic processes are important biological processes in the treatment of DKD by *** docking confirmed the excellent binding abilities of boldine,denudatin B,kaempferide,and quercetin to EGFR and *** results of in vitro experiments showed that treatment with an ethanolic extract of SQP significantly protected HK-2 cells from high glucose-induced cell *** addition,the SQP ethanol extract inhibited the phosphorylation of EGFR and SRC,suppressed the apoptosis rate,and regulated apoptosis-related proteins in HK-2 cells under hig

关键词： traditional Chinese medicine diabetic kidney disease Suoquan pill network analysis molecular docking

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