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Ethacrynic acid targets GSTM1 to ameliorate obesity by promoting browning of white adipocytes

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Protein & Cell 2021年第6期12卷 493-501页

作者： Zhaomeng Cui Yang Liu Wei Wan Yuyan Xu yehui hu Meng Ding Xin Dou Ruina Wang Hailing Li Yongmei Meng Wei Li Wei Jiang Zengxia Li Yiming Li Minjia Tan Dengke KMa Yu Ding Jun OLiu Cheng Luo Biao Yu Qiqun Tang Yongjun Dang Key Laboratory of Metabolism and Molecular Medicine The Ministry of EducationDepartment of Biochemistry and Molecular BiologyShanghai Medical CollegeFudan UniversityShanghai 200030China Institute of Stem Cell Research and Regenerative Medicine Institutes of Biomedical SciencesFudan UniversityShanghai 200030China State Key Laboratory of Drug Research Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China Department of Physiology and Biophysics School of Life SciencesFudan UniversityShanghai 200438China Henan Sesame Research Center Henan Academy of Agricultural SciencesZhengzhou 450002China College of Traditional Mongolian Medicine Inner Mongolia Medical UniversityMongolia 010110China State Key Laboratory of Bio-organic and Natural Products Chemistry Shanghai Institute of Organic ChemistryChinese Academy of SciencesShanghai 200032China Department of Endocrinology and Metabolism Huashan HospitalFudan UniversityShanghai 200040China Department of Physiology Cardiovascular Research InstituteUniversity of California San FranciscoSan FranciscoUSA Department of Pharmacology and Molecular Sciences The Johns Hopkins University School of MedicineBaltimoreMDUSA

Dear Editor,Obesity is caused by an imbalance between energy intake and expenditure,and has become a global epidemic with over 650 million adults *** tissues in mammals are composed of white adipose tissue(WAT)and classical brown adipose tissue(BAT),and their balance is highly related to the occurrence of *** browning of white adipocytes results in“beige”or“brite”adipocytes,which appear functionally similar to classical brown adipocytes,and can be detected in WAT deposits of animals that have been exposed to cold or other inducers(Fu et al.,2015).

关键词： GSTM1 obesity adipocyte

油茶茶籽壳中茶皂素分离纯化及结构分析的研究

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中国粮油学报 2023年第3期38卷 131-138页

作者：胡叶慧吴世照李国华浙江工业大学化学工程学院杭州310032

以油茶茶籽壳为原料,采用乙醇提取法对其皂素类物质进行粗提取,HPD-100大孔树脂吸附法对茶皂素粗提物进行纯化,利用紫外光谱和超高效液相色谱-质谱法对纯化样品进行鉴定。实验结果表明,最佳提取工艺参数为:料液比1∶15,乙醇体积分数75%... 详细信息

以油茶茶籽壳为原料,采用乙醇提取法对其皂素类物质进行粗提取,HPD-100大孔树脂吸附法对茶皂素粗提物进行纯化,利用紫外光谱和超高效液相色谱-质谱法对纯化样品进行鉴定。实验结果表明,最佳提取工艺参数为:料液比1∶15,乙醇体积分数75%,提取温度70℃,提取时间120 min,其最高提取率达25.58%。粗提物经HPD-100大孔树脂纯化,30 mg/mL粗提液上样,水和20%乙醇洗去极性大的杂质,80%乙醇溶液洗脱出茶皂素,茶皂素纯化得率为48.63%,纯度提高1.38倍。该工艺操作简单,适合于茶皂素的分离。纯化茶皂素通过紫外光谱和液质联用检测,结果显示该样品为52种茶皂素单体的混合物并得到了其在质谱中的裂解规律。

关键词：油茶茶皂素分离纯化结构分析

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绿色荧光蛋白结合蛋白的表达纯化与鉴定

复旦学报（自然科学版） 2019年第2期58卷 183-190页

作者：胡业辉李益鹏王紫英陈勖任真丁澦复旦大学生命科学学院生理学与生物物理学系上海200438

绿色荧光蛋白(GFP)及其衍生物已经被广泛应用于生物化学和分子生物学研究,其纳米抗体(GBP)也已在近期被鉴定,但GBP大规模表达及纯化流程优化尚未见报道,并且GBP与不同荧光蛋白之间的亲和力尚未被测定.我们首先构建了GBP基因(GBP1)的原... 详细信息

绿色荧光蛋白(GFP)及其衍生物已经被广泛应用于生物化学和分子生物学研究,其纳米抗体(GBP)也已在近期被鉴定,但GBP大规模表达及纯化流程优化尚未见报道,并且GBP与不同荧光蛋白之间的亲和力尚未被测定.我们首先构建了GBP基因(GBP1)的原核表达系统,然后通过Ni-NTA亲和层析和Mono Q阴离子交换层析纯化,每升菌液能够得到超过43mg、纯度超过95%的重组GBP1蛋白.我们通过MALDI-TOF/TOF质谱法测定纯化的GBP1的精确分子量,通过微量热泳动(MST)和等温滴定量热(ITC)实验检测GBP1与7种不同来源荧光蛋白的亲和力.结果显示GBP1特异性地与源自Aequorea victoria的荧光蛋白及其衍生荧光蛋白相互作用,与RFP和Zoanthus GFP等无相互作用.GBP1与EGFP和sfGFP的亲和力最高,与YPet和T-Sapphire的亲和力稍弱,与CyPet的亲和力最低.

关键词：荧光蛋白绿色荧光蛋白结合蛋白纳米抗体蛋白表达蛋白纯化微量热泳动

气相色谱-质谱法测定儿童玩具中18种多环芳烃

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塑料工业 2022年第12期50卷 117-121,169页

作者：李智王晓娟龚学红胡叶慧卢波李国华应海松邓云宁波海关技术中心浙江宁波315048 宁波检验检疫科学技术研究院浙江宁波315048 浙江工业大学化学工程学院浙江杭州310032

建立了气相色谱-质谱测定儿童玩具中18种多环芳烃(PAH)含量的方法。采用超声提取法提取儿童玩具中18种PAH,通过气相色谱-质谱法检测,外标法定量。对色谱柱、程序升温条件等GC-MS分析条件以及提取溶剂、提取时间、提取温度等提取条件进... 详细信息

建立了气相色谱-质谱测定儿童玩具中18种多环芳烃(PAH)含量的方法。采用超声提取法提取儿童玩具中18种PAH,通过气相色谱-质谱法检测,外标法定量。对色谱柱、程序升温条件等GC-MS分析条件以及提取溶剂、提取时间、提取温度等提取条件进行了优化,对方法性能进行了考察。18种PAH质量浓度在0.005~1.000 mg/L范围内,与其对应峰面积呈良好线性关系,检出限(3S/N)为0.001~0.014 mg/kg,加标回收率在85.2%~108.6%之间,相对标准偏差(n=6)为1.3%~9.3%。该方法前处理简单、灵敏度高、重现性好,能够满足儿童玩具中多环芳烃的检测要求。

关键词：多环芳烃儿童玩具气相色谱-质谱法

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芍花鲜花饼的研制与工艺优化

农产品加工 2023年第7期 45-49页

作者：权春梅王婧胡业慧吕艳燕亳州职业技术学院安徽亳州236800 安徽中医药大学安徽合肥230000

以可食用芍花、白砂糖、面粉等作为主要原料,研究芍花鲜花饼的制作并优化其制作工艺。研究芍花添加量、白砂糖添加量、糯米粉添加量对芍花鲜花饼馅料感官品质的影响,使用单因素试验和正交试验,得出馅料最优配比。将馅料配比固定,研究油... 详细信息

以可食用芍花、白砂糖、面粉等作为主要原料,研究芍花鲜花饼的制作并优化其制作工艺。研究芍花添加量、白砂糖添加量、糯米粉添加量对芍花鲜花饼馅料感官品质的影响,使用单因素试验和正交试验,得出馅料最优配比。将馅料配比固定,研究油酥皮中的玉米油添加量、烘烤温度、烘烤时间对芍花鲜花饼感官品质的影响。结果表明,优化后的芍花鲜花饼制作工艺配方为馅料中芍花9 g,糯米粉12 g,白砂糖20 g,油酥皮中的玉米油23 g,烘烤时间20 min,烘烤温度180℃。此工艺下,制作的芍花鲜花饼口感良好,芍花清香,饼皮有层次,外形圆整,口感软糯,甜度适中。

关键词：芍花芍花鲜花饼感官评价工艺优化

Influence of mesoscale eddies on primary production in the South China Sea during spring inter-monsoon period

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Acta Oceanologica Sinica 2014年第3期33卷 118-128页

作者： hu Zifeng TAN yehui SONG Xingyu ZHOU Linbin LIAN Xiping huANG Liangmin HE Yinghui Key Laboratory of Marine Bio-resources Sustainable Utilization (LMB) South China Sea Institute of Oceanology Chinese Academy of Sciences University of Chinese Academy of Sciences Marine Biology Research Station at Daya Bay Chinese Academy of Science State Key Laboratory of Tropical Oceanology (LTO) South China Sea Institute of Oceanology ChineseAcademy of Sciences

Mesoscale eddies have been suggested to have an impact on biological carbon fixation in the South China Sea （SCS）. However, their overall contribution to primary production during the spring inter-monsoon pe riod is still unknown. Based on large-scale biological and environmental in situ observations and synchro nous remote sensing data, the distribution patterns of phytoplankton biomass and the primary production, and the role of mesoscale eddies in regulating primary production in different eddy-controlled waters were investigated. The results suggested that the surface chlorophyll a concentrations and water column inte grated primary production （IPP） are significantly higher in cyclonic eddies and lower in the anticyclonic eddies as compared to that in non-eddy waters. Although eddies could affect various environmental factors, such as nutrients, temperature and light availability, nutrient supply is suggested to be the most important one through which mesoscale eddies regulated the distribution patterns of phytoplankton biomass and pri mary production. The estimated IPP in cyclonic and anticyclonic eddies are about 29.5% higher and 16.6% lower than the total average in the whole study area, respectively, indicating that the promotion effect of mesoscale cold eddies on the primary production was much stronger than the inhibition effect of the warm eddies per unit area. Overall, mesoscale eddies are crucial physical processes that affect the biological car bon fixation and the distribution pattern of primary production in the SCS open sea, especially during the spring inter-monsoon period.

关键词： mesoscale eddies chlorophyll a primary production vertically generalized production model,remote sensing South China Sea

Distribution of reactive aluminum under the influence of mesoscale eddies in the western South China Sea

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Acta Oceanologica Sinica 2017年第6期36卷 95-103页

作者： LIU Jiaxing ZHOU Linbin TAN yehui WANG Qionga hu Zifeng LI Jiajun JIANG Xin KE Zhixin Key Laboratory of Tropical Marine Bio-resources and Ecology South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301China Guangdong Provincial Key Laboratory of Applied Marine Biology South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301China University of Chinese Academy of Sciences Beijing 100049China

To understand the distribution of aluminum （A1） under the influence of mesocale eddies in the western South China Sea （SCS）, sea level anomaly, geostrophic current, environmental parameters and reactive A1 were investigated in the western SCS in August 2013. The highest reactive A1 concentration （（180±64） nmol/L） was observed in the surface waters, indicating a substantial atmospheric input. Vertically, the reactive A1 decreased from the surface high concentration to the subsurface minima at the depth of chlorophyll a （Chl a） maxima and then increased again with depth at most of the stations. The average concentration of reactive A1 in the upper 100 m water column was significantly lower in the cyclonic eddy （（137±6） nmol/L） as compared with that in the non- eddy waters （（180±21） nmol/L）. By contrast, the average concentrations of Chl a and silicate in the upper 100 m water column were higher in the cyclonic eddy and lower in the anticyclonic eddy. There was a significant negative correlation between the average concentrations of reactive Al and Chl a in the upper 100 m water column. The vertical distribution of reactive AI and the negative correlation between reactive A1 and Chl a both suggest that the reactive A1 in the upper water column was significantly influenced by biological removal processes. Our results indicate that mesoscale eddies could regulate the distribution of reactive A1 by influencing the primary production and phytoplankton community structure in the western SCS.

关键词： reactive aluminum mesoscale eddy chlorophyll a biological removal phytoplankton primaryproduction western South China Sea

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脆肉罗非鱼皮胶原蛋白肽的性质研究

中国食品添加剂 2024年第6期35卷 70-76页

作者：刘琳胡清雨郑佳楠张业辉焦文娟佘文海黄文广东省农业科学院蚕业与农产品加工研究所农业农村部功能食品重点实验室广东省农产品加工重点实验室广州510610 广州禄仕食品有限公司广州510820 广东省农业科学院动物科学研究所广州510645

提取脆肉罗非和普通罗非鱼皮中胶原蛋白肽,通过紫外光谱、荧光光谱、扫描电镜和SDS-PAGE凝胶电泳等方法对其性质进行对比研究。结果表明:普通和脆肉罗非鱼皮胶原蛋白肽中芳香族氨基酸含量均较高,红外光谱特征相似,吸收峰位于酰胺A带(341... 详细信息

提取脆肉罗非和普通罗非鱼皮中胶原蛋白肽,通过紫外光谱、荧光光谱、扫描电镜和SDS-PAGE凝胶电泳等方法对其性质进行对比研究。结果表明:普通和脆肉罗非鱼皮胶原蛋白肽中芳香族氨基酸含量均较高,红外光谱特征相似,吸收峰位于酰胺A带(3419.84 cm^(-1))、酰胺B带(2924.14 cm^(-1))和酰胺Ⅱ带(1554.15 cm^(-1)),且在25 KDa处有一条胶原蛋白肽电泳条带。扫描电镜结果发现普通罗非鱼皮胶原蛋白肽呈片状、较松散,而脆肉罗非鱼皮胶原蛋白肽交联度高、排列较紧密。脆肉罗非鱼皮胶原蛋白肽中人体必需氨基酸含量和氨基酸总量分别为37.61%和292.35 mg/g,均高于普通罗非鱼皮胶原蛋白肽。脆肉罗非鱼皮胶原蛋白肽的变性温度为63.83℃,普通罗非鱼皮胶原蛋白肽为60.67℃。脆肉罗非鱼皮胶原蛋白肽性质更为稳定。本研究可为鱼皮胶原蛋白肽的开发利用提供参考。

关键词：脆肉罗非鱼胶原蛋白肽特性

PhaseⅠdose-escalation and expansion study of PARP inhibitor,fluzoparib(SHR3162),in patients with advanced solid tumors

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Chinese Journal of Cancer Research 2020年第3期32卷 370-382页

作者： huiping Li Rongrui Liu Bin Shao Ran Ran Guohong Song Ke Wang yehui Shi Jihong Liu Wenjing hu Fu Chen Xiaoran Liu Gairong Zhang Chuanhua Zhao Ru Jia Quanren Wang Hope S.Rugo Yifan Zhang Guangze Li Jianming Xu Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education/Beijing) Department of Breast OncologyPeking University Cancer Hospital&InstituteBeijing 100142China Department of Gastrointestinal Oncology the Fifth Medical Center of Chinese PLA General HospitalBeijing 100071China Department of Gynecological Oncology Tianjin Medical University Cancer Institute&HospitalTianjin 300060China Departnient of Gynecologic Oncology Sun Yat-sen University Cancer CentreGuangzhou 510060China The Comprehensive Cancer Center of Drum Tower Hospital the Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjing 210008China Department of Clinical Medicine-Oncology Jiangsu Hengrui Medicine Co.Ltd.Shanghai 201203China Department of Medicine University of California San Francisco Comprehensive Cancer CenterSan Francisco 94127USA State Key Laboratory of Drug Research Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China

Objective:Fluzoparib(SHR3162)is a novel,potent poly(ADP-ribose)polymerases(PARP)1,2 inhibitor that showed anti-tumor activity in xenograft *** conducted a phaseⅠ,first-in-human,dose-escalation and expansion(D-Esc and D-Ex)trial in patients with advanced solid ***:This was a 3+3 phaseⅠD-Esc trial with a 3-level D-Ex at 5 hospitals in *** patients for DEsc had advanced solid tumors refractory to standard therapies,and D-Ex enrolled patients with ovarian cancer(OC).Fluzoparib was administered orally once or twice daily(bid)at 11 dose levels from 10 to 400 mg/*** included dose-finding,safety,pharmacokinetics,and antitumor ***:Seventy-nine patients were enrolled from March,2015 to January,2018[OC(47,59.5%);breast cancer(BC)(16,20.3%);colorectal cancer(8,10.1%),other tumors(8,10.1%)];48 patients were treated in the D-Esc arm and 31 in the D-Ex *** maximum tolerated dose(MTD)was 150 mg bid,with a half-life of 9.14 *** 3/4 adverse events included anemia(7.6%)and neutropenia(5.1%).The objective response rate(ORR)was 30%(3/10)in patients with platinum-sensitive OC and 7.7%(1/13)in patients with *** patients treated with fluzoparib≥120 mg/d,median progression-free survival(m PFS)was 7.2[95%confidence interval(95%CI),1.8-9.3]months in OC,9.3(95%CI,7.2-9.3)months in platinum-sensitive OC,and 3.5(range,2.0-28.0)months in *** patients with germline BC susceptibility gene mutation(g BRCAMut)(11/43 OC;2/16 BC),m PFS was 8.9 months for OC(range,1.0-23.2;95%CI,1.0-16.8)and 14 and 28 months for BC(those two patients both also had somatic BRCAMut).Conclusions:The MTD of fluzoparib was 150 mg bid in advanced solid *** demonstrated single-agent antitumor activity in BC and OC,particularly in BRCAMut and platinum-sensitive OC.

关键词： PhaseⅠ PARP inhibitor(fluzoparib) solid tumor pharmacokinetics safety antitumor activity