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Molecular characterization and developmental expression patterns of thyroid hormone receptors(TRs) and their responsiveness to TR agonist and antagonist in Rana nigromaculata

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Journal of Environmental Sciences 2014年第10期26卷 2084-2094页

作者： Qinqin Lou Yinfeng Zhang Dongkai Ren Haiming Xu yaxian zhao Zhanfen Qin Wuji Wei State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-environmental SciencesChinese Academy of Sciences College of Environment Nanjing University of Technology

Considering some advantages of Rana nigromaculata as an experimental species, we propose that this species, like Xenopus laevis, could be used to assay thyroid hormone（TH） signaling disrupting actions. To validate the utilizability of R. nigromaculata, we investigated the responsiveness of R. nigromaculata to a TH receptor（TR） agonist（T3） and antagonist（amiodarone） by analyzing expression, based on characterizing TR cDNA and developmental expression patterns. With high levels of identity with the corresponding genes in X. laevis, both TRα and TRβ in R. nigromaculata exhibited roughly similar developmental expression patterns to those of X. laevis, in spite of some species-specific differences. Both TRα and TRβ expression had greater changes in the liver and intestine than in the tail and brain during metamorphosis. T3 exposure for 2 days induced more dramatic increases of TRβ expression in stage 27 than in stage34 tadpoles but not in stage 42 tadpoles, showing that the responsiveness of R. nigromaculata to TH decreased with development and disappeared at the onset of metamorphic *** to greater changes of TRβ expression in the liver and intestine than in the tail and brain during metamorphosis, the liver and intestine had higher responsiveness to exogenous T3 than the tail and brain. Amiodarone inhibited T3-induced TRβ expression. Our results show that R. nigromaculata can be used as a model species for assaying TH signaling disrupting actions by analyzing TRβ expression, and intestine tissues at stage 27 are ideal test materials due to high responsiveness and easy accessibility.

关键词： Rana nigromaculata Thyroid hormone receptor Thyroid hormone signaling Developmental expression pattern Responsiveness

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Polybrominated diphenyl ethers in chicken tissues and eggs from an electronic waste recycling area in southeast China

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Journal of Environmental Sciences 2011年第1期23卷 133-138页

作者： Xiaofei Qin Zhanfen Qin Yan Li yaxian zhao Xijuan Xia Shishuai Yan Mi Tian Xingru zhao Xiaobai Xu Yongjian Yang State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-environmental Sciences Chinese Academy of Sciences Beijing 100085 China Chinese Research Academy of Environmental Sciences Beijing 100012 China Taizhou No. 1 Middle School Taizhou 318000 China

The levels and distributions of polybrominated diphenyl ethers （PBDEs） in chicken tissues from an electronic waste （e-waste） recycling area in southeast China were investigated. Human dietary intake by local residents via chicken muscle and eggs was estimated. The mean PBDEs concentrations in tissues ranged from 15.2 to 3138.1 ng/g lipid weight （lw） and in egg the concentration was 563.5 ng/g lw. The results showed that the level of total PBDEs （∑PBDEs） in the chicken tissue was 2-3 orders of magnitude higher than those reported in the literature. The large difference of ∑PBDEs concentrations between tissues confirmed that the distribution of PBDEs in tissues depend on tissue-specificity rather than the ＂lipid-compartment＂. BDE-209 was the predominant congener （82.5%- 94.7% of ∑PBDEs） in all chicken tissues except in brain （34.7% of ∑PBDEs）, which indicated that deca-BDE （the major commercial PBDE formulation comprising 65%-70% of total production） was major pollution source in this area and could be bioaccumulated in terrestrial animals. The dietary PBDEs intake of the local residents from chicken muscle and egg, assuming only local bred chickens and eggs were consumed, ranged from 2.2 to 22.5 ng/（*** body weight （bw）） with a mean value of 13.5 ng/（day-kg bw）, which was one order of magnitude higher than the value reported in previous studies for consumption of all foodstuffs.

关键词： polybrominated diphenyl ethers chicken egg bioaccumulation tissue distribution exposure risk

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Induction of gyno-tetraploidy in Japanese fl ounder Paralichthys olivaceus

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Journal of Oceanology and Limnology 2020年第1期38卷 288-293页

作者： WANG Guixing ZHANG Xiaoyan SUN zhaohui zhao yaxian DU Wei CUI Jianbin HOU Jilun WANG Yufen Key Laboratory of Aquatic Genomics Ministry of AgricultureBeijing 100141China Beidaihe Central Experiment Station Chinese Academy of Fishery SciencesQinhuangdao 066100China Ocean College Agricultural University of HebeiQinhuangdao 066009China

Tetraploid fish are important for mass production of triploids in polyploid *** this study,we reported a novel protocol for artificial induction of tetraploidy by a combination of cold shock and hydrostatic pressure administered to gynogenetically developed eggs in Japanese flounder Paralichthys *** induction was carried out by activating the eggs with UV-irradiated sperm of red sea bream Pagrus major,administering a cold shock(0℃,5 to 45 min)3 min after fertilization to inhibit second polar body exclusion,incubating the eggs for 60 min at 17℃,and treating them with a 650 kg/cm2 hydrostatics pressure shock for 6 *** named the embryos gyno-tetraploids that developed from eggs after such *** hatching rate of the gyno-tetraploids ranged from 20.99%±3.66%to 36.01%±2.79%,and the tetraploid rate ranged from 80.00%to 100.00%.All-maternal inheritance was verified using 6 high-recombination-rate microsatellite *** method successfully induced *** successful induction of gyno-tetraploidy lays the foundation for triploidization of new varieties with improved economic traits of interest that can benefit commercial culture.

关键词： gynogenesis polyploidy chromosome manipulation mosaic

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Ecotoxicological effects of mixed pollutants resulted from e-wastes recycling and bioaccumulation of polybrominated diphenyl ethers in Chinese loach(Misgurnus anguillicaudatus)

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Journal of Environmental Sciences 2009年第12期21卷 1695-1701页

作者： QIN Xiaofei XIA Xijuan LI Yan zhao yaxian YANG Zhongzhi FU Shan TI-AN Mi zhao Xingru QIN Zhanfen XU Xiaobai YANG Yongjian State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-environmental Sciences Chinese Academy of Sciences Beijing 100085 China Taizhou No.1 Middle School Taizhou 318000 China

To understand potential ecotoxicological effects of electrical and electronic equipment waste （e-waste） recycling and polybrominated diphenyl ethers （PBDEs） bioaccumulation in loaches, a semi-field experiment using Chinese loach （Misgurnus anguiUicaudatus） as experimental fish was performed. Larval loaches were kept in net-cage for three months in an e-wastes recycling site and a reference site in Southeastern China. There was significant difference of the survival rate between the loaches from the e-wastes recycling site （27%, 19/70） and from reference site （70%, 49/70）. Histopathological responses were also found in all the livers examined in loaches from the e-wastes recycling site. These results showed that mixed pollutants resulted from e-wastes recycling led to ecotoxicological effects on loaches. The bioaccumulation of polybrominated diphenyl ethers （PBDEs）, the main pollutants in e-waste, in loaches was also studied, the mean concentration of total PBDEs in sediment was 6726.17 ng/g wet weight and in water samples was 4.08 ng/L （dissolved phase）. BDE 209 was the dominant congener in sediment and with relatively high concentration in water. Relatively low concentration of BDE 209 （less than 0.01% of total PBDEs） and high concentration of BDE47 （up to 39.34% of total PBDEs） were detected in loaches.

关键词： e-wastes loach ecotoxicological effects polybrominated diphenyl ethers semi-field experiment

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Thyroid disruption by technical decabromodiphenyl ether (DE-83R) at low concentrations in Xenopus laevis

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Journal of Environmental Sciences 2010年第5期22卷 744-751页

作者： Xiaofei Qin Xijuan Xia Zhongzhi Yang Shishuai Yan yaxian zhao Rongguo Wei Yan Li Mi Tian Xingru zhao Zhanfen Qin Xiaobai Xu State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-environmental Sciences Chinese Academy of Sciences Beijing 100085 China Chinese Research Academy of Environmental Sciences Beijing 100012 China

Decabromodiphenyl ether （decaBDE）,as a flame retardant,is widely produced and *** study the thyroid disruption by technical decaBDE at low concentrations,Xenopus laevis tadpoles were exposed to technical decaBDE mixture DE-83R （1-1000 ng/L） in water from stage 46/47 （free swimming larvae,system of Nieuwkoop and Faber） to stage ***-83R at concentration of 1000 ng/L significantly delayed the time to metamorphosis （presented by forelimb emergence,FLE）.Histological examination showed that DE83R at all tested concentrations caused histological alterations-multilayer follicular epithelial cell and markedly increased follicle size accompanied by partial colloid depletion and increase in the peripheral colloid vacuolation,in thyroid *** tested concentrations of DE-83R also induced a down-regulation of thyroid receptor mRNA *** results demonstrated that technical decaBDE disrupted the thyroid system in *** *** of polybrominated diphenyl ethers （PBDEs）（sum of 39 congeners） in *** indicated that mean concentrations of total PBDEs in *** exposed to 1,10,100,1000 ng/L were 11.0,128.1,412.1,1400.2 ng/g wet weight,*** that PBDEs burden of *** tadpoles was close to PBDEs levels in amphibians as reported in previous studies,our study has raised new concerns for thyroid disruption in amphibians of technical decaBDE at environmentally relevant concentrations.

关键词： decabromodiphenyl ether Xenopus laevis thyroid disruption metamorphosis thyroid hormone receptor

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Polybrominated diphenyl ether (PBDE) in blood from children (age 9–12) in Taizhou, China

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Journal of Environmental Sciences 2011年第7期23卷 1199-1204页

作者： Xiaoyou Zhang Xianli Ruan Minchan Yan yaxian zhao Wuji Wei Zhanfen Qin Yongjian Yang Haiming Xu Yan Li Nanjing University of Technology Nanjing 210009 China State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-environmental Sciences Chinese Academy of Sciences Beijing 100085 China Taizhou Hospital Taizhou 317000 China Tongde Hospital Hangzhou 310012 China Taizhou No. 1 Middle School Taizhou 318000 China

Polybrominated diphenyl ethers （PBDEs） as ubiquitous persistent organic pollutants have attracted much attention in recent years. Exposure to PBDEs could induce a high health risk for children. The aim of this study was to investigate the PBDEs exposure of children （9–12 years） from Taizhou, China. Fifty-eight blood samples were collected in one school in a mountainous area in Taizhou. The concentrations of ∑9PBDEs （sum of BDE-28, -47, -99, -100, -153, -154, -183, -197 and -209） ranged from 2.66 to 33.9 ng/g lipid wet （lw） with a median of 7.22 ng/g lw. These concentrations were lower than those of children in USA, but close to European and Asian general population levels. The results showed that children in Taizhou countryside were at a low level of PBDEs exposure. The predominant congener was BDE-209, followed by BDE-28, -47, -197 and -153. High abundance of BDE-209 was consistent with the pollution background of PBDEs in China characterized by high brominated congeners as main pollutants.

关键词： polybrominated diphenyl ether children blood exposure Taizhou

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化学链高温空分制氧性能评价

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东南大学学报（自然科学版） 2013年第4期43卷 809-813页

作者：赵亚仙向文国陈时熠东南大学能源与环境学院南京210096

采用Aspen Plus软件对高温空分制氧流程进行模拟研究并做出评价.基于热力学平衡原理,对不同金属氧化物载体(Mn3O4-Mn2O3和CoO-Co3O4)、不同反应温度下的平衡氧分压进行计算,得到了Mn3O4-Mn2O3和CoO-Co3O4体系氧平衡分压与温度的关系;在... 详细信息

采用Aspen Plus软件对高温空分制氧流程进行模拟研究并做出评价.基于热力学平衡原理,对不同金属氧化物载体(Mn3O4-Mn2O3和CoO-Co3O4)、不同反应温度下的平衡氧分压进行计算,得到了Mn3O4-Mn2O3和CoO-Co3O4体系氧平衡分压与温度的关系;在计算平衡氧分压的基础上,确定了制氧所需的空气量与水量/CO2量;构建了制氧流程,并进行了模拟计算.结果表明,在2种载氧体Mn3O4-Mn2O3和CoO-Co3O4中,载氧体Mn3O4-Mn2O3制氧性能较好;化学链高温空分制取纯氧能耗为0.353 kW.h/kg,与传统制氧能耗经验数据0.246 kW.h/kg相比并不具有明显的优势;化学链高温空分制取O2和CO2混合气体能耗为0.151 kW.h/kg(氧气体积分数为14.3%),与传统制氧能耗经验数据0.246 kW.h/kg相比,具有节能潜力.

关键词：化学链空分制氧载氧体制氧能耗水蒸气二氧化碳

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H_2O_2改性对聚丙烯腈原丝化学结构的影响

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复合材料学报 2019年第1期36卷 85-95页

作者：赵雅娴武帅康宸刘倓徐樑华北京化工大学碳纤维及功能高分子教育部重点实验室北京100029

为节省预氧化进程的能耗和时间并优化聚丙烯腈(PAN)预氧纤维的性能,用H_2O_2改性PAN原丝,使其提前环化。采用FTIR、XPS等方法表征不同处理温度获得的未改性和改性PAN原丝。结果表明:H_2O_2水溶液在60℃改性PAN原丝时,H_2O_2可引发氰基环... 详细信息

为节省预氧化进程的能耗和时间并优化聚丙烯腈(PAN)预氧纤维的性能,用H_2O_2改性PAN原丝,使其提前环化。采用FTIR、XPS等方法表征不同处理温度获得的未改性和改性PAN原丝。结果表明:H_2O_2水溶液在60℃改性PAN原丝时,H_2O_2可引发氰基环化,末端环发生亚胺、烯胺互变异构,由此出现亚氰基、类芳香伯胺;改性温度越高,改性PAN原丝的亚氰基含量、共轭程度越大。在模拟稳定化过程中,改性PAN原丝的类芳香伯胺可在较低温度下引发相邻氰基环化。使用氨水(NH3·H2O)作为助剂获得改性PAN原丝,与未改性PAN原丝经历相同的预氧化进程,改性后的PAN原丝能在较短时间内达到适合的预氧化程度,且PAN预氧纤维径向结构的均匀性被改善,由此获得热稳定性更高的PAN预氧纤维。

关键词：聚丙烯腈原丝 H2O2 改性氮结构环化反应径向结构

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化学链高温空分制氧与IGCC集成系统性能评价

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热力发电 2016年第4期45卷 95-99页

作者：赵亚仙向文国晋中学院机械学院山西晋中030600 东南大学能源与环境学院江苏南京210096

基于Aspen plus软件,建立了化学链高温空分制氧与IGCC集成的系统(CLAS-IGCC)模型,并以蒸汽作为化学链高温空分载氧介质,从载氧蒸汽过热度、氧煤质量比(氧煤比)、水煤质量比(水煤比)和载氧体等4个方面对CLAS-IGCC的性能进行了研究。结果... 详细信息

基于Aspen plus软件,建立了化学链高温空分制氧与IGCC集成的系统(CLAS-IGCC)模型,并以蒸汽作为化学链高温空分载氧介质,从载氧蒸汽过热度、氧煤质量比(氧煤比)、水煤质量比(水煤比)和载氧体等4个方面对CLAS-IGCC的性能进行了研究。结果表明:CLAS-IGCC效率随载氧蒸汽过热度增加而先减小后增大,随氧煤比增大而减小;水煤比对系统效率影响不大;以Mn_3O_4-Mn2O_3为载氧体的CLAS-IGCC与以CoO-Co_3O_4为载氧体的CLAS-IGCC相比略具优势;CLAS-IGCC效率为45.1%,而采用常规低温空分制氧技术的IGCC效率为45.8%,因此,蒸汽型CLAS-IGCC集成效果不具优势。

关键词：化学链高温空分制氧 IGCC 蒸汽过热度氧煤比水煤比载氧体系统效率

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中小学音乐教育中的传统音乐教学策略

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文化产业 2019年第11期 63-64页

作者：赵雅娴永安市北塔学校

在我国新课改不断深化的背景下,中小学音乐教育成为新课改的重要阵地之一。新课改背景下的中小学音乐教育工作,应提高传统音乐文化元素的比重,适当应用流行音乐素材,提升传统音乐校本课程建设速度;要重视对传统音乐的审美体验,重视利用... 详细信息

在我国新课改不断深化的背景下,中小学音乐教育成为新课改的重要阵地之一。新课改背景下的中小学音乐教育工作,应提高传统音乐文化元素的比重,适当应用流行音乐素材,提升传统音乐校本课程建设速度;要重视对传统音乐的审美体验,重视利用多媒体进行传统音乐学习,并使音乐教育与日常生活实际紧密结合,提升学生综合素质,促进学生全面发展。

关键词：中小学音乐教育传统音乐

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