Induction of gyno-tetraploidy in Japanese fl ounder Paralichthys olivaceus

作     者：WANG Guixing ZHANG Xiaoyan SUN Zhaohui ZHAO Yaxian DU Wei CUI Jianbin HOU Jilun WANG Yufen WANG Guixing;ZHANG Xiaoyan;SUN Zhaohui;ZHAO Yaxian;DU Wei;CUI Jianbin;HOU Jilun;WANG Yufen

作者机构：Key Laboratory of Aquatic GenomicsMinistry of AgricultureBeijing 100141China Beidaihe Central Experiment StationChinese Academy of Fishery SciencesQinhuangdao 066100China Ocean CollegeAgricultural University of HebeiQinhuangdao 066009China 

出 版 物：《Journal of Oceanology and Limnology》 (海洋湖沼学报（英文）)

年 卷 期：2020年第38卷第1期

页      面：288-293页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071002[理学-动物学] 

基　　金：Supported by the Central Public-Interest Scientific Institution Basal Research Fund,CAFS(No.2016HY-ZD0202) the China Agriculture Research System(No.CARS-47) the Applied National Science and Technology Support Program in China(No.2012BAD26B01) 

主　　题：gynogenesis polyploidy chromosome manipulation mosaic 

摘      要：Tetraploid fish are important for mass production of triploids in polyploid *** this study,we reported a novel protocol for artificial induction of tetraploidy by a combination of cold shock and hydrostatic pressure administered to gynogenetically developed eggs in Japanese flounder Paralichthys *** induction was carried out by activating the eggs with UV-irradiated sperm of red sea bream Pagrus major,administering a cold shock(0℃,5 to 45 min)3 min after fertilization to inhibit second polar body exclusion,incubating the eggs for 60 min at 17℃,and treating them with a 650 kg/cm2 hydrostatics pressure shock for 6 *** named the embryos gyno-tetraploids that developed from eggs after such *** hatching rate of the gyno-tetraploids ranged from 20.99%±3.66%to 36.01%±2.79%,and the tetraploid rate ranged from 80.00%to 100.00%.All-maternal inheritance was verified using 6 high-recombination-rate microsatellite *** method successfully induced *** successful induction of gyno-tetraploidy lays the foundation for triploidization of new varieties with improved economic traits of interest that can benefit commercial culture.