Prothoracicotropic hormone has been successfully extracted from as many as 2,800,000 silkworm male adult heads and purified partially through an 8-step procedure. Approximately 7μg of the crude preparation ('crude P...
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Prothoracicotropic hormone has been successfully extracted from as many as 2,800,000 silkworm male adult heads and purified partially through an 8-step procedure. Approximately 7μg of the crude preparation ('crude PTTH') shows activity in the debrained Samia pupa test. A further 4-step chromatographic purification of the 'crude PTTH' derived from 100,000 Bombyx heads yields 3.4mg of purified preparation ('highly purified PTTH'), of which 7ng causes adult development in a debrained Samia pupa.
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