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Heteromerization of short-chain trans-prenyltransferase controls precursor allocation within a plastidial terpenoid network

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Journal of Integrative Plant Biology 2023年第5期65卷 1170-1182页

作者： Yihua Ma Qingwen Chen yaoyao wang Fengxia Zhang Chengyuan wang Guodong wang State Key Laboratory of Plant Genomics Institute of Genetics and Developmental BiologyThe Innovative Academy of Seed DesignChinese Academy of SciencesBeijing 100101China UniversityofChineseAcademyof Sciences Beijing100039China Center for Microbes Development and HealthInstitut Pasteur of ShanghaiChinese Academy of SciencesShanghai 200031China

Terpenes are the largest and most diverse class of plant specialized ***(C25),which are derived from the plastid methylerythritol phosphate pathway,were recently characterized in *** Arabidopsis thaliana,four genes encoding geranylfarnesyl diphosphate synthase(GFPPS)(AtGFPPS1 to 4)are responsible for the production of GFPP,which is the common precursor for sesterterpene ***,the interplay between sesterterpenes and other known terpenes remain ***,we first provide genetic evidence to demonstrate that GFPPSs are responsible for sesterterpene production in *** of the sesterterpene pathway at the GFPPS step increased the production of geranylgeranyl diphosphate(GGPP)-derived ***,co-expression of sester TPSs in GFPPSOE(overexpression)plants rescued the phenotypic changes of GFPPS-OE plants by restoring the endogenous *** further demonstrated that,in addition to precursor(DMAPP/IPP)competition by GFPPS and GGPP synthase(GGPPS)in plastids,GFPPS directly decreased the activity of GGPPS through protein-protein interaction,ultimately leading to GGPP deficiency in *** study provides a new regulatory mechanism of the plastidial terpenoid network in plant cells.

关键词： Arabidopsis geranylfarnesyl diphosphate synthase geranylgeranyl pyrophosphate synthase plastid sesterterpene terpene network

Research on Restoration of Murals Based on Diffusion Model and Transformer

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Computers, Materials & Continua 2024年第9期80卷 4433-4449页

作者： yaoyao wang Mansheng Xiao Yuqing Hu Jin Yan Zeyu Zhu School of Computing Hunan University of TechnologyZhuzhou412000China School of Computing Hunan Software Vocational and Technical UniversityXiangtan411100China

Due to the limitations of a priori knowledge and convolution operation,the existing image restoration techniques cannot be directly applied to the cultural relics mural restoration,in order to more accurately restore the original appearance of the cultural relics mural images,an image restoration based on the denoising diffusion probability model(Denoising Diffusion Probability Model(DDPM))and the Transformer *** process involves two steps:in the first step,the damaged mural image is firstly utilized as the condition to generate the noise image,using the time,condition and noise image patch as the inputs to the noise prediction network,capturing the global dependencies in the input sequence through the multi-attentionmechanismof the input sequence and feedforward neural network processing,and designing a long skip connection between the shallow and deep layers in the Transformer blocks between the shallow and deep layers using long skip connections to fuse the feature information of global and local outputs to maintain the overall consistency of the restoration results;In the second step,taking the noisy image as a condition to direct the diffusion model to back sample to generate the restored *** results show that the PSNR and SSIM of the proposedmethod are improved by 2%to 9%and 1%to 3.3%,respectively,which are compared to the comparison *** study proposed synthesizes the advantages of the diffusionmodel and deep learningmodel to make themural restoration results more accurate.

关键词： Transformer deep learning noise estimation network diffusion model mural restoration

Effects of Enteromorpha prolifera polysaccharides on growth performance,intestinal barrier function and cecal microbiota in yellow-feathered broilers under heat stress

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Journal of Animal Science and Biotechnology 2024年第1期15卷 344-361页

作者： Wenchao Liu Huimei Liu yaoyao wang Zhongxiang Zhao Balamuralikrishnan Balasubramanian Rajesh Jha Department of Animal Science College of Coastal Agricultural SciencesGuangdong Ocean UniversityZhanjiangGuangdong 524088People’s Republic of China Department of Food Science and Biotechnology College of Life ScienceSejong UniversitySeoul 05006South Korea Department of Human Nutrition Food and Animal SciencesCollege of Tropical Agriculture and Human ResourcesUniversity of Hawaii at ManoaHonoluluHI 96822USA

Background Global warming leading to heat stress(HS)is becoming a major challenge for broiler *** study aimed to explore the protective effects of seaweed(Enteromorpha prolifera)polysaccharides(EPS)on the intestinal barrier function,microbial ecology,and performance of broilers under HS.A total of 144 yellow-feathered broilers(male,56 days old)with 682.59±7.38 g were randomly assigned to 3 groups:1)TN(thermal neutral zone,23.6±1.8℃),2)HS(heat stress,33.2±1.5℃ for 10 h/d),and 3)HSE(HS+0.1%EPS).Each group contained 6 replicates with 8 broilers per *** study was conducted for 4 weeks;feed intake and body weights were measured at the end of weeks 2 and *** the end of the feeding trial,small intestine samples were collected for histomorphology,antioxidant,secretory immunoglobulin A(s Ig A)content,apoptosis,gene and protein expression analysis;cecal contents were also collected for microbiota analysis based on 16S r DNA *** Dietary EPS promoted the average daily gain(ADG)of broilers during 3–4 weeks of HS(P<0.05).At the end of HS on broilers,the activity of total superoxide dismutase(T-SOD),glutathione S-transferase(GST),and the content of s Ig A in jejunum were improved by EPS supplementation(P<0.05).Besides,dietary EPS reduced the epithelial cell apoptosis of jejunum and ileum in heat-stressed broilers(P<0.05).Addition of EPS in HS group broilers'diet upregulated the relative m RNA expression of Occludin,ZO-1,γ-GCLc and IL-10 of the jejunum(P<0.05),whereas downregulated the relative m RNA expression of NF-κB p65,TNF-αand IL-1βof the jejunum(P<0.05).Dietary EPS increased the protein expression of Occludin and ZO-1,whereas it reduced the protein expression of NF-κB p65 and MLCK(P<0.01)and tended to decrease the protein expression of TNF-α(P=0.094)in heat-stressed ***,the proportions of Bacteroides and Oscillospira among the three groups were positively associated with jejunal apoptosis and pro-inflammatory cytokine ex

关键词： Broilers Cecal microbiota Heat stress Intestinal barrier function Seaweed polysaccharides

Stem CO_(2) efflux of Abies fabri in subalpine forests in the Gongga Mountains,Eastern Tibetan Plateau

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Journal of Plant Ecology 2017年第6期10卷 1001-1011页

作者： Guang Zhao Gangcai Liu Wanze Zhu Jixia Zhao Xuemei wang yaoyao wang Min Jia Institute of Mountain Hazards and Environment Chinese Academy of Sciences and Ministry of Water Resources#.9Block 4Renminnanlu RoadChengdu 610041China University of Chinese Academy of Sciences 19A YuquanluBeijing 100039China

Aims Despite the importance of stem cO_(2)efflux(Es)in ecosystem carbon cycling and energy balance,little is known about temporal variation in the temperature coefficient(Q10)and sapwood nitrogen concen-trations([N])and their intrinsic links with *** objectives of this study were:(i)to examine the response of Es to temperature in a subalpine region and(ii)to explore the influence of stem diameter and[N]on ***,we will test the hypothesis that(i)Es in trees has thermal acclimation and(ii)Es will be well correlated with diameter at breast height(DBH)and[N].Methods Here,a horizontally oriented soil chamber technique was applied to measure Es of Abies fabri in two subalpine forest stands in Southwestern china from May to December *** also exam-ined the variability in Es,Q10 and[N]in trees and monitored the relationship between temperature,[N],DBH and *** Findings During the measurement period,Es showed an apparent seasonal trend,following the change in air temperature,increasing from May and peaking in July,then continuously decreasing until *** mean Es for the growing and dormant seasons were 1.45 and 0.25μmol·m^(−2)·s^(−1),respectively,and Es in the mature forest was sig-nificantly higher than in the immature *** area-based Es was positively correlated with DBH and sapwood width(SW),while volume-based Es showed negative relationship with DBH and *** the five diameter classes,69.8-89.0%of the variation in Es could be explained by air *** temperature sensitiv-ity(Q10)of Es ranged from 2.98 to 5.61 during the measurement period,with a higher Q10 appearing in the growing season than in the dormant *** was a significant linear relationship(P<0.01)between[N]and Es(expressed based on two different units).Additionally,exponential models of Es against[N]and air temperature were applied to estimate Es.

关键词： stem cO_(2)efflux Q10 DBH sapwood nitrogen contents subalpine forests

Kuroshio Intrusion Combined with Coastal Currents Affects Phytoplankton in the Northern South China Sea Revealed by Lipid Biomarkers

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Journal of Ocean University of China 2023年第2期22卷 576-586页

作者： wang yaoyao BI Rong GAO Jiawei ZHANG Hailong LI Li DING Yang JIN Gui’e ZHAO Meixun Frontiers Science Center for Deep Ocean Multispheres and Earth System and Key Laboratory of Marine Chemistry Theory and TechnologyMinistry of EducationOcean University of ChinaQingdao 266100China Laboratory for Marine Ecology and Environmental Science Qingdao National Laboratory for Marine Science and TechnologyQingdao 266237China

The northern South China Sea(NSCS)is significantly influenced by the Kuroshio intrusion and the coastal *** knowledge on the roles of both currents on phytoplankton spatial variations is still ***,we investigated the concentrations of phytoplankton biomarkers and their proportions in surface suspended particles from 47 sites of the NSCS during summer of 2017 and ***/epi-brassicasterol,dinosterol,and C37 alkenones were used as proxies of biomass for diatoms,dinoflagellates,and haptophytes,respectively,and their sum indicating total phytoplankton biomass.A three end-member mixing model was applied to quantitatively assess the influence extent of the Kuroshio intrusion and the coastal *** results showed that the Kuroshio intrusion and the coastal currents contributed equally to the overall surface water masses in the study area;however,the two currents had distinct effects on the spatial distribution of *** phytoplankton biomass,the eutrophic coastal currents were likely to be the main controlling factors,while the impact of the Kuroshio intrusion was weak and stimulated significant increases in phytoplankton biomass only at certain boundary *** phytoplankton community structures,the Kuroshio and its intrusion were the main factors,resulting in an increase in the proportions of dinoflagellates and *** proportion of diatoms slightly increased due to the influence of the coastal *** study quantifies the effects of the Kuroshio and the coastal currents on phytoplankton in the NSCS in terms of hydrological parameters,providing an important basis for the understanding of ecological functions and biogeochemical cycles in marginal sea-open ocean boundary regions.

关键词： sterols alkenones phytoplankton northern South China Sea Kuroshio coastal currents

Highly stable carbon-coated nZVI composite Fe0@RF-C for efficient degradation of emerging contaminants

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Environmental Science and Ecotechnology 2024年第6期22卷 233-244页

作者： Guizhou Xu Lin Sun Yizhou Tu Xiaolei Teng Yumeng Qi yaoyao wang Aimin Li Xianchuan Xie Xueyuan Gu State Key Laboratory of Pollution Control and Resource Reuse School of the EnvironmentNanjing UniversityNanjing210023China Key Laboratory of Poyang Lake Environment and Resource Utilization Engineering Research Center of Watershed Carbon NeutralizationMinistry of EducationSchool of Resource and EnvironmentNanchang UniversityNanchang330031China Nanjing University&Yancheng Academy of Environment Protection Technology and Engineering Nanjing210023China Jiangxi Nanxin Environmental Protection Technology Co.LTD Jiujiang CityJiangxi Province330300China

Nanoscale zerovalent iron(nZVI)has garnered significant attention as an efficient advanced oxidation activator,but its practical application is hindered by aggregation and *** nZVI with carbon can effectively addresses these issues.A simple and scalable production method for carbon-coated nZVI composite is highly *** anti-oxidation and catalytic performance of carbon-coated nZVI composite merit in-depth *** this study,a highly stable carbon-coated core-shell nZVI composite(Fe0@RF-C)was successfully prepared using a simple method combining phenolic resin embedding and carbothermal ***0@RF-C was employed as a heterogeneous persulfate(PS)activator for degrading 2,4-dihydroxybenzophenone(BP-1),an emerging *** to commercial nZVI,Fe0@RF-C exhibited superior PS activation performance and oxidation *** 95%of BP-1 was removed within 10 min in the Fe0@RF-C/PS *** carbon layer promotes the enrichment of BP-1 and accelerates its degradation through singlet oxygen oxidation and direct electron transfer *** study provides a straightforward approach for designing highly stable carbon-coated nZVI composite and elucidates the enhanced catalytic performance mechanism by carbon layers.

关键词： Nanoscale zerovalent iron Carbon-coated nZVI Persulfate BP-1 Enhanced degradation

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分组激振旋转超声电机弹性振动分析

天津大学学报（自然科学与工程技术版） 2016年第11期49卷 1203-1208页

作者：王世宇王尧尧天津大学机械工程学院天津300072 天津市非线性动力学与混沌控制重点实验室天津300072

为解决电机受传统时空相位限制的这一难题,针对超声电机的激振问题,采用能量方法建立了定子弹性动力学模型.应用解析方法研究了压电片的分组对称拓扑对弹性振动特性的影响,揭示了拓扑结构与行波及驻波之间的映射关系,得到了产生行波的... 详细信息

为解决电机受传统时空相位限制的这一难题,针对超声电机的激振问题,采用能量方法建立了定子弹性动力学模型.应用解析方法研究了压电片的分组对称拓扑对弹性振动特性的影响,揭示了拓扑结构与行波及驻波之间的映射关系,得到了产生行波的一般条件.结果表明:采用满足该映射关系的工作波数、压电片个数及其分组可生成期望的波动响应;该映射关系提供了保证振幅最大的压电片最优分组方式.研究结果有利于优化激振方式,提高超声定子的激振效率.

关键词：超声电机分组对称激振方式

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小剪跨比锈蚀钢筋混凝土梁受剪性能试验研究

建筑结构学报 2019年第12期40卷 147-154页

作者：杨晓明吴桐王耀耀辽宁工程技术大学土木工程学院

钢筋混凝土梁中箍筋发生锈蚀易导致结构性能退化以至失效破坏,为此针对HRB400钢筋作为箍筋发生锈蚀后的钢筋混凝土梁在剪跨比为1. 5以下的受剪性能的退化展开研究。制作8根钢筋混凝土梁,按照剪跨比为1. 5和1分成两组进行试验,比较未锈... 详细信息

钢筋混凝土梁中箍筋发生锈蚀易导致结构性能退化以至失效破坏,为此针对HRB400钢筋作为箍筋发生锈蚀后的钢筋混凝土梁在剪跨比为1. 5以下的受剪性能的退化展开研究。制作8根钢筋混凝土梁,按照剪跨比为1. 5和1分成两组进行试验,比较未锈蚀和锈蚀后的钢筋性能以及试验梁的受剪承载力。研究结果表明:定义箍筋竖直段的锈蚀率为有效锈蚀率进行分析,能够有效反映箍筋的锈蚀程度和截面损失情况;箍筋有效锈蚀率小于20%时钢筋混凝土梁受剪承载力下降并不明显,当箍筋有效锈蚀率大于30%时受剪承载力下降明显,且剪跨比越大钢筋混凝土梁受剪承载力下降幅度越大;剪跨比小于1. 5时,锈蚀箍筋性能退化对钢筋混凝土梁受剪承载力下降影响较小,受剪承载力下降主要是因为箍筋锈蚀引发锈胀裂缝导致核心混凝土整体性下降所致。

关键词：钢筋混凝土梁小剪跨比箍筋锈蚀有效锈蚀率锈胀裂缝静力试验受剪性能

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市域铁路桥梁标准墩设计研究

城市轨道交通研究 2023年第S2期26卷 59-63页

作者：王瑶瑶中铁上海设计院集团有限公司上海200070

目前,我国市域铁路桥梁标准墩的设计研究相对缺乏。为此,以某上海市域铁路工程为背景,开展桥梁标准墩的设计研究。首先,以花瓶墩为基础形式,结合人文景观要求,确定了镂空花瓶墩为标准墩的造型;通过静力和抗震计算确定了桥墩尺寸和基础布... 详细信息

目前,我国市域铁路桥梁标准墩的设计研究相对缺乏。为此,以某上海市域铁路工程为背景,开展桥梁标准墩的设计研究。首先,以花瓶墩为基础形式,结合人文景观要求,确定了镂空花瓶墩为标准墩的造型;通过静力和抗震计算确定了桥墩尺寸和基础布置;基于顶帽受力复杂的特点,建立实体有限元计算模型,计算结果表明顶帽跨中处有较大的主拉应力,因此设计时采取加强配筋的方式改善受力;针对桥址区高烈度及柔性场地的特点,提出一种新型榫形防落梁装置,计算表明设置防落梁装置的标准墩减震率可达到68%~78%,有效地提高了桥梁的抗震设防能力。

关键词：市域铁路桥梁标准墩标准墩设计减隔震技术