Heteromerization of short-chain trans-prenyltransferase controls precursor allocation within a plastidial terpenoid network

作     者：Yihua Ma Qingwen Chen Yaoyao Wang Fengxia Zhang Chengyuan Wang Guodong Wang Yihua Ma;Qingwen Chen;Yaoyao Wang;Fengxia Zhang;Chengyuan Wang;Guodong Wang

作者机构：State Key Laboratory of Plant GenomicsInstitute of Genetics and Developmental BiologyThe Innovative Academy of Seed DesignChinese Academy of SciencesBeijing 100101China UniversityofChineseAcademyof SciencesBeijing100039China Center for MicrobesDevelopment and HealthInstitut Pasteur of ShanghaiChinese Academy of SciencesShanghai 200031China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2023年第65卷第5期

页      面：1170-1182页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0903[农学-农业资源与环境] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 

基　　金：financially supported by the National Key R&D Program of China(2018YFA0900600) the National Natural Science Foundation of China(31970315) the Key R&D Program of Shandong Province(2019JZZY020610) the State Key Laboratory of Plant Genomics of China(grant no.SKLPG2016A-13) 

主　　题：Arabidopsis geranylfarnesyl diphosphate synthase geranylgeranyl pyrophosphate synthase plastid sesterterpene terpene network 

摘      要：Terpenes are the largest and most diverse class of plant specialized ***(C25),which are derived from the plastid methylerythritol phosphate pathway,were recently characterized in *** Arabidopsis thaliana,four genes encoding geranylfarnesyl diphosphate synthase(GFPPS)(AtGFPPS1 to 4)are responsible for the production of GFPP,which is the common precursor for sesterterpene ***,the interplay between sesterterpenes and other known terpenes remain ***,we first provide genetic evidence to demonstrate that GFPPSs are responsible for sesterterpene production in *** of the sesterterpene pathway at the GFPPS step increased the production of geranylgeranyl diphosphate(GGPP)-derived ***,co-expression of sester TPSs in GFPPSOE(overexpression)plants rescued the phenotypic changes of GFPPS-OE plants by restoring the endogenous *** further demonstrated that,in addition to precursor(DMAPP/IPP)competition by GFPPS and GGPP synthase(GGPPS)in plastids,GFPPS directly decreased the activity of GGPPS through protein-protein interaction,ultimately leading to GGPP deficiency in *** study provides a new regulatory mechanism of the plastidial terpenoid network in plant cells.