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De novo domestication towards new crops

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National Science Review 2021年第4期8卷 40-41页

作者： Xianrong Xie yao-guang liu State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources South China Agricultural University guangdong Laboratory for Lingnan Modern Agriculture College of Life Sciences South China Agricultural University

Long-term natural and human selections have domesticated crops from their wild progenitors for millennia, leading to the fixation of a large number of agronomically beneficial alleles and traits in cultivated crops. However, this process has been extremely time-consuming, accompanied with negative consequences, such as genetic erosion,

关键词： scanning tunneling microscopy phenyl-based molecular film electronic correlation electron-vibration coupling Kondo effect

Long non-coding RNAs play an important role in regulating photoperiod-and temperature-sensitive male sterility in rice

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Science China(Life Sciences) 2017年第4期60卷 443-444页

作者： Jingxin Guo yao-guang liu State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Key Laboratory of Plant Functtonal Genomicsand Biotechnology of Guangdong Provincial Higher Education Institutions College of Life Sciences South China Agricultural University Guangzhou 510642 China

Rice(Oryza sativa L.)is a major food crop for more than half of the world’s ***,increasing rice production is of great *** successful development of hybrid rice in the 1970s was an important achievement for increasing yield potential,since hybrid rice varieties produce higher yields than inbred varieties(Cheng et al.,2007).In plants,male sterility refers to the inability of producing dehiscent anthers and functional pollen(Chen and liu,2014).

关键词： sterility inbred pollen alleles inability producing recessive fertility regulating sterile

DELLA-GRF4-mediated coordination of growth and nitrogen metabolism paves the way for a new Green Revolution

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Science China(Life Sciences) 2018年第9期61卷 1130-1131页

作者： Kun Ma yao-guang liu State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources College of Life Sciences South China Agricultural University

The Green Revolution （GR） based on semi-dwarf breeding has greatly increased cereal crop yields since the 1960＇*** to appropriate plant height, the semi-dwarf crop plants are beneficial to increase light utilization and improve the lodging-resistance, and eventually increase yield potential （Hedden, 2003）. During the past 50 years, the worldwide application of semi-dwarf crop breeding has produced plenty of elite varieties. The characters of Green Revolution varieties （GRVs） are caused by the mutant growth-repressing genes.

关键词：新陈代谢格林生长道路协作世界范围氮庄稼

Molecular Control of Male Reproductive Development and Pollen Fertility in Rice

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Journal of Integrative Plant Biology 2012年第12期54卷 967-978页

作者： Jing-Xin Guo yao-guang liu State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources College of Life SciencesSouth China Agricultural University

Anther development and male fertility are essential biological pro- cesses for flowering plants and are important for crop seed produc- tion. Genetic manipulation of male fertility/sterility is critical for crop hybrid breeding. Rice （Oryza sativa L.） male sterility phenotypes, including genic male sterility, hybrid male sterility, and cytoplasmic male sterility, are generally caused by mutations of fertility-related genes, by incompatible interactions between divergent allelic or non-allelic genes, or by genetic incompatibilities between cytoplas-mic and nuclear genomes. Here, we review the recent advances in the molecular basis of anther development and male fertility-sterility conversion in specific genetic backgrounds, and the interactions with certain environmental factors. The highlighted findings in this review have significant implications in both basic studies and rice genetic improvement.

关键词： Anther development cytoplasmic male sterility hybrid sterility male fertility meiosis rice.

Rice Mitochondrial Genes Are Transcribed by Multiple Promoters That Are Highly Diverged

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Journal of Integrative Plant Biology 2006年第12期48卷 1473-1477页

作者： Qun-Yu Zhang yao-guang liu Key Laboratory of Plant Functional Genomics and Biotechnology of guangdong College of Life Sciences South China Agricultural University Guangzhou 510642 China

Plant mitochondrial genes are often transcribed into complex sets of mRNA. To characterize the transcription initiation and promoter structure, the transcript termini of four mitochondrial genes, atpl, atp6, cob, rps7, in rice （Oryza sativa L.）, were determined by using a modified circularized RNA reverse transcription- polymerase chain reaction method. The results revealed that three genes （atp1, atp6, rps7） were transcribed from multiple initiation sites, indicating the presence of multiple promoters. Two transcription termination sites were detected in three genes （atp6, cob, rps7）, respectively. Analysis on the promoter architecture showed that the YRTA （Y=T or C, R=A or G） motifs that are widely present in the mitochondrlal promoters of other monocot and dicot plant species were detected only in two of the 12 analyzed promoters. Our data suggest that the promoter sequences in the rice mitochondrial genome are highly diverged in comparison to those in other plants, and the YRTA motif is not an essential element for the promoter activity.

关键词： mitochondrial gene promoter rice transcription initiation site transcription termination site

Genome-editing technologies: the gap between application and policy

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Science China(Life Sciences) 2019年第11期62卷 1534-1538页

作者： Gousi Li yao-guang liu Yuanling Chen State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources South China Agricultural UniversityGuangzhou 510642China Key Laboratory of Plant Functional Genomics and Biotechnology of guangdong Provincial Higher Education Institutions South China Agricultural UniversityGuangzhou 510642China College of Life Sciences South China Agricultural UniversityGuangzhou 510642China

Genome editing is a new technology for manipulating genomic DNA sequences at specific sites,and researchers worldwide are rapidly developing new variants of genomeediting applications that target mRNAs,viruses,chromat... 详细信息

关键词： Genome processes. editing

Assessing protein-DNA interactions: Pros and cons of classic and emerging techniques

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Science China(Life Sciences) 2016年第4期59卷 425-427页

作者： Yongyao Xie yao-guang liu Letian Chen State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources South China Agricultural University guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms South China Agricultural University Key Laboratory of Plant Functional Genomics and Biotechnology of guangdong Provincial Higher Education Institutions South China Agricultural University College of Life Sciences South China Agricultural University

Investigation of protein-DNA interactions provides important information for understanding gene function and regulation, but identification and validation of specific interactions remain major challenges in the post-genomics era. Therefore, effective and economical methods to assess protein-DNA interactions are highly sought-after by molec- ular biologists.

关键词： DNA classic genomics sought challenges economical emerging agarose sequencing interacting

Structural and Expressional Variations of the Mitochondrial Genome Conferring the Wild Abortive Type of Cytoplasmic Male Sterility in Rice

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Journal of Integrative Plant Biology 2007年第6期49卷 908-914页

作者： Zhen-Lan liu Hong Xu Jing-Xin Guo yao-guang liu Key Laboratory of Plant Functional Genomics and Biotechnology of Education Department Guangdong Province College of Life Sciences South China Agricultural University Guangzhou 510642 China

The so-called ＂wild abortive＂（WA） type of cytoplasmic male sterility （CMS） derived from a wild rice species Oryza rufipogon has been extensively used for hybrid rice breeding. However, extensive analysis of the structure of the related mitochondrial genome has not been reported, and the CMS-associated gene（s） remain unknown. In this study, we exploited a mitochondrial genome-wide strategy to examine the structural and expressional variations in the mitochondrial genome conferring the CMS. The entire mitochondriai genomes of a CMS-WA line and two normal fertile rice lines were amplified by Long-polymerase chain reaction into tilling fragments of up to 15.2 kb. Restriction and DNA blotting analyses of these fragments revealed that structural variations occurred in several regions in the WA mitochondrial genome, as compared to those of the fertile lines. All of the amplified fragments covering the entire mitochondrial genome were used as RNA blot probes to examine the mitochondriai expression profile among the CMS-WA and fertile lines. As a result, only two mRNAs were found to be differentially expressed between the CMS-WA and the fertile lines, which were detected by a probe containing the nad5 and orf153 genes and the other having the ribosomal protein gene rpl5, respectively. These mRNAs are proposed to be the candidates for further identification and functional studies of the CMS gene.

关键词： cytoplasmic male sterility mitochondrial genome rice transcription.

Peripheral nerve repair: a hot spot analysis on treatment methods from 2010 to 2014

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Neural Regeneration Research 2015年第6期10卷 996-1002页

作者： guang-yao liu Yan Jin Qiao Zhang Rui Li Department of Orthopedics China-Japan Union Hospital of Jilin University Department of Ophthalmology the Second Hospital of Jilin University Hand & Foot Surgery and Reparative & Reconstruction Surgery Center the Second Hospital of Jilin University

Therapeutic strategies for neurological deficits and for promoting nerve regeneration after peripheral nerve injuries have received much focus in clinical research. Advances in basic research in recent years have increased our understanding of the anatomy of peripheral nerves and the importance of the microenvironment. Various new intervention methods have been developed, but with varying effectiveness. In the present study, we selected 911 papers on different repair methods for peripheral nerve injury from the Web of Science and indexed in the Science Citation Index from 2010 to 2014. We quantitatively examine new repair methods and strategies using bibliometrics, and we discuss the present state of knowledge and the problems and prospects of various repair methods, including nerve transfer, neural transplantation, tissue engineering and genetic engineering. Our findings should help in the study and development of repair methods for peripheral nerve injury.

关键词： nerve regeneration peripheral nerve injury nerve repair neural transplantation nervetransfer tissue engineering genetic engineering bibliometrics Web of Science neural regeneration