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Differential gene and protein expression between rat tibial nerve and common peroneal nerve during wallerian degeneration

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Neural Regeneration Research 2019年第12期14卷 2183-2191页

作者： Yao-Fa Lin Zheng Xie Jun Zhou Gang Yin Hao-Dong lin Department of Orthopedic Surgery Changzheng Hospital the Second Military Medical University Shanghai China Department of Orthopedic Surgery Shanghai General Hospital Shanghai Jiao Tong University School of Medicine Shanghai China

Wallerian degeneration and nerve regeneration after injury are complex processes involving many genes, proteins and cytokines. After different peripheral nerve injuries the regeneration rate can differ. Whether this is caused by differential expression of genes and proteins during Wallerian degeneration remains unclear. The right tibial nerve and the common peroneal nerve of the same rat were exposed and completely cut through and then sutured in the same horizontal plane. On days 1, 7, 14, and 21 after surgery, 1–2 cm of nerve tissue distal to the suture site was dissected out from the tibial and common peroneal nerves. The differences in gene and protein expression during Wallerian degeneration of the injured nerves were then studied by RNA sequencing and proteomic techniques. In the tibial and common peroneal nerves, there were 1718, 1374, 1187, and 2195 differentially expressed genes, and 477, 447, 619, and 495 differentially expressed proteins on days 1, 7, 14, and 21 after surgery, respectively. Forty-seven pathways were activated during Wallerian degeneration. Three genes showing significant differential expression by RNA sequencing (Hoxd4, Lpcat4 and Tbx1) were assayed by real-time quantitative polymerase chain reaction. RNA sequencing and real-time quantitative polymerase chain reaction results were consistent. Our findings showed that expression of genes and proteins in injured tibial and the common peroneal nerves were significantly different during Wallerian degeneration at different time points. This suggests that the biological processes during Wallerian degeneration are different in different peripheral nerves after injury. The procedure was approved by the Animal Experimental Ethics Committee of the Second Military Medical University, China (approval No. CZ20160218) on February 18, 2016.

关键词： nerve regeneration peripheral nerves peripheral nerve injuries Wallerian degeneration tibial nerve common peroneal nerve RNA sequencing proteomic real-time quantitative polymerase chain reaction neural regeneration

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Effect of exogenous spastin combined with polyethylene glycol on sciatic nerve injury

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Neural Regeneration Research 2019年第7期14卷 1271-1279页

作者： Yao-Fa Lin Zheng Xie Jun Zhou Hui-Hao Chen Wan-Wan Shao Hao-Dong lin Department of Orthopedics Changzheng Hospitalthe Second Military Medical University Department of Orthopedics the Second People's Hospital of Karamay

Polyethylene glycol can connect the distal and proximal ends of an injured nerve at the cellular level through axonal fusion to avoid Wallerian degeneration of the injured distal nerve and promote peripheral nerve regeneration.However,this method can only prevent Wallerian degeneration in 10% of axons because the cytoskeleton is not repaired in a timely fashion.Reconstruction of the cytoskeletal trunk and microtubule network has been suggested to be the key for improving the efficiency of axonal fusion.As a microtubule-severing protein,spastin has been used to enhance cytoskeletal reconstruction.Therefore,we hypothesized that spastin combined with polyethylene glycol can more effectively promote peripheral nerve regeneration.A total of 120 male Sprague-Dawley rats were randomly divided into sham,suture,polyethylene glycol,and polyethylene glycol + spastin groups.In suture group rats,only traditional nerve anastomosis of the end-to-end suture was performed after transection of the sciatic nerve.In polyethylene glycol and polyethylene glycol + spastin groups,50 μL of polyethylene glycol or 25 μL of polyethylene glycol + 25 μL of spastin,respectively,were injected immediately under the epineurium of the distal suture.Sensory fiber regeneration distance,which was used to assess early nerve regeneration at 1 week after surgery,was shortest in the suture group,followed by polyethylene glycol group and greatest in the polyethylene glycol + spastin group.Behavioral assessment of motor function recovery in rats showed that limb function was restored in polyethylene glycol and polyethylene glycol + spastin groups at 8 weeks after surgery.At 1,2,4 and 8 weeks after surgery,sciatic functional index values and percentages of gastrocnemius muscle wet weight were highest in the sham group,followed by polyethylene glycol + spastin and polyethylene glycol groups,and lowest in the suture group.Masson staining was utilized to assess the morphology of muscle tissue.Morphological changes in skeletal muscle were detectable in suture,p

关键词： nerve regeneration peripheral nerves Wallerian degeneration polyethylene glycol axonal fusion spastin peripheral nerve injuries Masson staining microtubule neural regeneration

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Molecular Mechanisms of Ventilator-Induced Lung Injury

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Chinese Medical Journal 2018年第10期131卷 1225-1231页

作者： lin Chen Hai-fa Xia You Shang Shang-Long yao Department of Anesthesiology Institute of Anesthesiology and Critical Care Medicine Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei 430022 China Department of Critical Care Medicine Institute of Anesthesiology and Critical Care Medicine Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei 430022 China

Objective：Mechanical ventilation （MV） has long been used as a life-sustaining approach for several decades.However,researchers realized that MV not only brings benefits to patients but also cause lung injury if used improperly,which is termed as ventilator-induced lung injury （VILI）.This review aimed to discuss the pathogenesis of VILI and the underlying molecular mechanisms.Data Sources：This review was based on articles in the PubMed database up to December 2017 using the following keywords：＂ventilator-induced lung injury＂,＂pathogenesis＂,＂mechanism＂,and ＂biotrauma＂.Study Selection：Original articles and reviews pertaining to mechanisms of VILI were included and reviewed.Results：The pathogenesis of VILI was defined gradually,from traditional pathological mechanisms （barotrauma,volutrauma,and atelectrauma） to biotrauma.High airway pressure and transpulmonary pressure or cyclic opening and collapse of alveoli were thought to be the mechanisms of barotraumas,volutrauma,and atelectrauma.In the past two decades,accumulating evidence have addressed the importance of biotrauma during VILI,the molecular mechanism underlying biotrauma included but not limited to proinflammatory cytokines release,reactive oxygen species production,complement activation as well as mechanotransduction.Conclusions：Barotrauma,volutrauma,atelectrauma,and biotrauma contribute to VILI,and the molecular mechanisms are being clarified gradually.More studies are warranted to figure out how to minimize lung injury induced by MV.

关键词： Biotrauma Mechanism Pathogenesis Ventilator-Induced Lung Injury

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Investigation of erosion-corrosion behavior of Q235B steel in liquid-solid flows

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Petroleum Science 2022年第5期19卷 2358-2373页

作者： Yan-lin Zhao Fu-Xiang Ye Ge Zhang Jun yao Yu-fa Liu Shi-Gang Dong International Joint Laboratory on Clean Energy Science and Technology Beijing Key Laboratory of Process Fluid Filtration and SeparationCollege of Mechanical and Transportation EngineeringChina University of PetroleumBeijing102249People’s Republic of China College of Energy Xiamen UniversityXiamen361005People’s Republic of China

This work aims to investigate the erosion-corrosion behavior of Q235B steel in liquid-solid two-phase flows.The weight loss rate,surface morphology and electrochemical parameters of Q235B steel at different temperatures(20℃,30℃,40℃)and flow velocities(6 m/s,7 m/s,8 m/s,9 m/s,10 m/s)were studied separately.The results show that the weight loss rate of Q235B steel specimens after erosion-corrosion increases with increasing flow velocity and temperature.For the erosion-corrosion process,the corrosion rates of specimens increase with increasing flow velocity.The results of surface morphology show that the circular pits with clear edges are distributed randomly over specimen surface at low flow velocity,but the pit edge becomes vague at high flow velocity.With temperature increasing,the erosion-corrosion damage became serious as shown by the aggregation of large and small pits on specimen surface.The working mechanism of erosion-corrosion is found to vary with flow velocity and temperature.The relationships among erosion-corrosion components are quantitatively represented and show that synergy dominates the progress of material loss.Corrosion enhances erosion that is a dominant component in the synergy.The inactions of erosion-corrosion can be described by"synergistic"and"additive"behavior.The results show that"additive"effect becomes more significant with increasing flow velocity but decreases with increasing temperature,while"synergistic"effect is not sensitive to flow velocity and temperature.

关键词： Erosion-corrosion Liquid-solid Synergy Flow velocity Temperature

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Photoelectron Spectroscopy, Photoionization Mass Spectroscopy, and Theoretical Study on CCl3SSCN

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Chinese Journal of Chemical Physics 2008年第2期21卷 93-98页

作者： lin Du Li yao Mao-fa Ge Beijing National Laboratory for Molecular Sciences State Key Laboratory for Structural Chemistry of Unstable and Stable Species Institute of Chemistry Chinese Academy of Sciences Beijing 100080 China Graduate School of Chinese Academy of Sciences Beijing 100039 China

Trichloromethanesulfenyl thiocyanate， CC13SSCN，被光电子产生并且学习光谱学(足) ，光电游离团光谱学(PIMS ) ，和理论计算。这个分子展出笨拙的符合构造，并且在 S-S 债券附近的扭力的角度是 91.4 湩朠湥牥污 ? 整浲吗？

关键词：电光子分光光谱光致电离作用硫氰酸盐光谱学

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Effect of diflubenzuron on the chitin biosynthesis pathway in Conopomorpha sinensis eggs

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Insect Science 2021年第4期28卷 1061-1075页

作者： Qiong yao lin-fa Quan Shu Xu Yi-Zhi Dong Wen-Jing Li Bing-Xu Chen Guangdong Provincial Key Laboratory of New High Technology for Plant Protection Plant Protection Research InstituteGuangdong Academy of Agricultural SciencesGuangzhou510640China Guangdong Laboratory for lingnan Modern Agriculture Guangzhou510642China

Conopomorpha sinensis is the dominant borer pest of Litchi chinensis(litchi)and Euphoria longan(longan)in China.Control of C.sinensis is difficult because of its cryptic life habit;thus,an effective ovicide could be beneficial.The larvicidal effects of diflubenzuron(DFB)have been documented in many insect pest species.Therefore,DFB might be a useful ovicide to control C.sinensis.However,the detailed mode of action of DFB interference with insect molting and egg hatching is unclear.Thus,we studied alterations in expression of all genes potentially affected by DFB treatment using a transcriptome approach in 2-d-old C.sinensis eggs.Clean reads were assembled to generate 203455 unigenes and 440558 transcripts.A total of 4625 differently expressed genes,which included 2670 up-regulated and 1955 down-regulated unigenes,were identified.Chitin binding and chitin metabolic processes were among the most significant enriched pathways according to Gene Ontology analyses.Most of the genes that encode enzymes involved in the chitin biosynthesis pathway were unaffected,whereas genes that presumably encode cuticle proteins were up-regulated.Furthermore,altered expression patterns of 10 genes involved in the chitin biosynthesis pathway of C.sinensis embryos were observed in response to DFB treatment at different time points by quantitative reverse transcription polymerase chain reaction.We also observed abnormal development;there was reduced chitin content and modulated chitin distribution of newly hatched larvae,and altered egg hatching.Our findings illustrate an ovicidal effect of DFB on C.sinensis,and reveal more molecular consequences of DFB treatment on insects.

关键词： Conopomorpha sinensis chitin diflubenzuron ovicidal effect transcriptomics

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Real role of growth factor receptor-binding protein 10:linking lipid metabolism to diabetes cardiovascular complications

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World Journal of Clinical Cases 2022年第35期10卷 12875-12879页

作者： Yang Yang Hua-Jie yao Wei-Jie lin Si-Chao Huang Xiao-Dong Li fa-Zhong He Department of Pharmacy Zhuhai People’s Hospital(Zhuhai Hospital Affiliated with Jinan University)Zhuhai 519000Guangdong ProvinceChina College of Life Science and Technology Wuhan Polytechnic UniversityWuhan 430000Hubei ProvinceChina Department of Quality Control Zhuhai People’s Hospital(Zhuhai Hospital Affiliated with Jinan University)Zhuhai 519000Guangdong ProvinceChina

Cardiovascular complications of patients with type 2 diabetes mellitus(T2DM)threaten the health and life of numerous individuals.Recently,growth factor receptor-binding protein 10(GRB10)was found to play a pivotal role in vascular complications of T2DM,which participates in the regulation of lipid metabolism of T2DM patients.The genetic variation of GRB10 rs1800504 is closely related to the risk of coronary heart disease in patients with T2DM.The development of GRB10 as a key mediator in the association of lipid metabolism with cardiovascular complications in T2DM is detailed in and may provide new potential concerns for the study of cardiovascular complications in T2DM patients.

关键词： Type 2 diabetes mellitus Growth factor receptor-binding protein 10 Vascular complications Lipid metabolism

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国际金融安全的旧疾新症及中国应对

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上海对外经贸大学学报 2023年第2期30卷 73-88页

作者：张发林姚远南开大学周恩来政府管理学院天津300350

国际经济和政治秩序的深度调整推动全球金融治理进入新时期,但在新旧国际金融风险相互交织的背景下,国际金融安全不仅没有得到有效改善,甚至有恶化的趋势。微观、中观和宏观层次的传统国际金融风险并未得到有效治理,反而在新冠疫情爆发... 详细信息

国际经济和政治秩序的深度调整推动全球金融治理进入新时期,但在新旧国际金融风险相互交织的背景下,国际金融安全不仅没有得到有效改善,甚至有恶化的趋势。微观、中观和宏观层次的传统国际金融风险并未得到有效治理,反而在新冠疫情爆发以来进一步加剧。微观层面,新冠疫情加剧了全球金融风险的积累,并推动国际金融监管弱化。中观和宏观层面,金融风险的国内和国际治理陷入停滞,具有高风险性的等级化国际金融结构得以延续,甚至强化。此外,金融保护主义和大国竞争增加了国际金融安全面临的新政治风险,国际金融“政治化”趋势逐渐凸显,具体表现为金融“安全化”和“武器化”倾向增强。中国需要从国家层面完善对金融安全的战略规划,并根据不同风险类型制定和优化应对策略,同时在国际层面倡导新型金融安全观和金融合作,从而维护国家金融安全与国际金融稳定。

关键词：国际金融安全国家金融安全全球金融治理

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The classification of intracranial aneurysm neck: a single center research experience

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Chinese Neurosurgical Journal 2019年第3期5卷 129-136页

作者： Cai-Qiang Huang De-Zhi Kang Liang-Hong Yu Shu-fa Zheng Pei-Sen yao Yuan-Xiang lin Zhang-Ya lin Department of Neurosurgery The First Affiliated HospitalFujian Medical UniversityNo.20 Changzhou RoadTaijiang DistrictFuzhou 350004Fujian ProvinceChina

There is associating with incidence of unfavorable outcomes compared to microsurgical clippings. We are in order to investigate the outcomes of microsurgical clipping for intracranial aneurysms and determine the ideal clipping methods for different aneurysm subtypes. Method: Retrospectively analyzed the clinical characteristics and follow-up data (completely recorded) of 123 patients with 128 aneurysms were treated. 20 cases were treated as control group from October 2013 to December 2013. Since January 2014, aneurysms were classified base on the 20 cases of aneurysm imaging data. 103 patients were treated as experimental group, the classification of aneurysms previously proposed was used to estimate the way of surgery, and the guiding value of the genotype was verified according to the intraoperative findings. The proposed aneurysm classification is based on the virtual surface of the aneurysm and the parent artery, the aneurysm neck was classified as follows: subtype I, the curved surface of the neck is a single curved surface;subtype II, the neck is hyperboloid;subtype III, neck is a three-curved surface. Aneurysms were divided into further subtypes according to the ratio of the width of the aneurysm neck surface and the length of the artery circumference: subtype A, the ratio of the aneurysm neck surface to the parent artery was not more than 0.5;subtype B, more than 0.5. There are some clamping methods include simple, sliding, interlocking and hybrid. Results: In the control group, patients did not undergo a suitable clipping scheme without classification of aneurysm neck (unclassed clipping). While causing the occurrence of occlusion adverse events, including neck residual, Tumor artery stenosis, electrophysiological changes, the lack of blood supply and so on. The experimental[page1image12073600]group was analyzed by using a predetermined clipping scheme (classed clipping), and the use of aneurysms clamps was approximately the same as expected. Compared the preoperative assessment with the actual situation

关键词： Intracranial aneurysms Microsurgery clipping Aneurysm neck classification Aneurysm neck curved surface

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Seismic monitoring network based on MEMS sensors

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Earthquake Science 2019年第3期32卷 179-185页

作者： Zhen-xuan Zou Ming Zhang Xu-dong He Sheng-fa lin Zheng-yao Dong Kan Sun Zhejiang Earthquake Agency Hangzhou 310013China Zhoushan Science and Technology Bureau Zhoushan 316000China

This paper provides a brief introduction to the application of the sensor monitoring network of micro-electro-mechanical systems(MEMS)to Zhejiang province.In the Wenzhou Shanxi reservoir and other areas,MEMS and traditional intensity-monitoring instruments have been deployed with complementary functions to implement hybrid networking.The low-cost MEMS network can continuously monitor areas at high risk of earthquakes at a high resolution.Moreover,it can quickly collect the parameters of earthquakes and records of the near-field acceleration of strong earthquakes.It can be also used to rapidly generate earthquake intensity reports and provide early warning of earthquakes.We used the MEMS sensors for the first time in 2016,and it has helped promote the development and application of seismic intensity instruments since then.

关键词： MEMS sensor seismic intensity instrument hybrid networking rapid intensity reports early warning

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