检索结果-南通市图书馆

An integrative review on the applications of 3D printing in the field of in vitro diagnostics

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2022年第5期33卷 2231-2242页

作者： Jian Yang yanxiang cheng Xia Gong Shengzhu Yi Cheuk-Wing Li Lelun Jiang Changqing Yi Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instrument School of Biomedical EngineeringSun Yat-sen UniversityShenzhen 518107China Research Institute of Sun Yat-sen University in Shenzhen Shenzhen 518057China School of Science and Technology Nottingham Trent UniversityClifton LaneNottingham NG118NSUnited Kingdom

Biomedicine is one of the fastest growing areas of additive ***,in the field of in vitro diagnostics(IVD),contributions of 3D printing include i)rapid prototyping and iterative IVD proof-of-concept designing ranging from materials,devices to system integration;ii)conceptual design simpli-fication and improved practicality of IVD products;iii)shifting the IVD applications from centralized labs to point-of-care testing(POCT).In this review,the latest developments of 3D printing and its advantages in IVD applications are summarized.A series of 3D-printed objects for IVD applications,including single-function modules,multi-function devices which integrate several single-function modules for specific an-alytical applications such as sample pre-treatment and chemo-/bio-sensing,and all-in-one systems which integrate multi-function devices and the instrument operating them,are analyzed from the perspective of functional *** current and potential commercial applications of 3D-printed objects in the IVD field are *** features of 3D printing,especially rapid prototyping and low start-up,en-able the easy fabrication of bespoke modules,devices and systems for a range of analytical applications,and broadens the commercial IVD prospects.

关键词： 3D printing In vitro diagnostics Portable sensing device Point-of-care testing Stereolithography(SLA) Digital light projection(DLP) Fused deposition modeling(FDM)

Synthesis,characterization and photoluminescence of aluminum N-aryloxo functionalized β-ketoiminate complexes

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2011年第14期56卷 1471-1475页

作者： FENG Tao PENG HongMei YAO YingMing ZHANG Yong SHEN Qi cheng yanxiang Key Laboratory of Organic Synthesis of Jiangsu Province College of Chemistry Chemical Engineering and Materials Science Soochow University Suzhou 215123 China State Key Laboratory of Polymer Physics and Chemistry Changchun Institute of Applied Chemistry. Chinese Academy of Sciences Changchun 130022. China

The synthesis,characterization and luminescent properties of aluminum complexes containing a dianionic N-aryloxo functionalized β-ketoiminate ligand are presented.4-(2-Hydroxy-5-R-phenyl)imino-2-pentanone (R=Me,L 1 H 2;R=tert-butyl,L 2 H 2) ligands reacted with AlEt 3 in tetrahydrofuran to give the aluminum complexes (L 1 AlEt) 2 (1) and (L 2 AlEt) 2 (2) in reasonable isolated yields.X-ray diffraction revealed that complexes 1 and 2 have solvent-free centrosymmetric dimeric structures,and each aluminum center has distorted trigonal bipyramidal *** room temperature,complexes 1 and 2 exhibit blue photoluminescence in acetonitrile with maximum emission wavelengths of 419 and 413 nm,respectively.

关键词：光致发光复合物合成表征铝发光性能四氢呋喃中心对称

Carbazole ring: A delicate rack for constructing thermally activated delayed fluorescent compounds with through-space charge transfer

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2021年第12期32卷 4011-4014页

作者： Kuofei Li Tao Wang Bing Yao Yuannan Chen Hao Deng Hongmei Zhan Zhiyuan Xie yanxiang cheng State Key Laboratory of Polymer Physics and Chemistry Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022China School of Applied Chemistry and Engineering University of Science and Technology of ChinaHefei 230026China

Three carbazole derivatives, Ac PTC, Px PTC and Pt PTC, consisting of two 9,9-dimethyl-9,10-dihydroacridine,phenoxazine or phenothiazine donor groups and one diphenyltriazine acceptor group fixed at 1,8,9-positions of a single carbazole ring via phenylene, are designed and synthesized. X-ray diffraction analysis of Ac PTC reveals that there exist multiple π-π interactions between the donor and acceptor groups to form a sandwich-like structural unit with edge-to-face interaction model. The compounds thus show obvious thermally activated delayed fluorescence with through-space charge transfer character and possess considerable photoluminescence quantum yields of up to 73% in doped films with sky-blue to yellow emissions. The solution-processed electroluminescent devices achieve the highest maximum external quantum efficiencies of 10.0%, 11% and 5.6% for Ac PTC, Px PTC and Pt PTC, respectively, with small efficiency roll-offs.

关键词： Thermally activated delayed fluorescence Through-space charge transfer Carbazole Solution-processed devices Sky-blue emission

The role of a drug-loaded poly(lactic co-glycolic acid)(PLGA) copolymer stent in the treatment of ovarian cancer

在线全文

学校读者我要写书评

暂无评论

Cancer Biology & Medicine 2020年第1期17卷 237-250页

作者： Yanqing Wang Xiaoyin Qiao Xiao Yang Mengqin Yuan Shu Xian Li Zhang Dongyong Yang Shiyi Liu Fangfang Dai Zhikai Tan yanxiang cheng Department of Obstetrics and Gynecology Renmin Hospital of Wuhan UniversityWuhan 430060China College of Biology Hunan UniversityChangsha 410082China Department of Obstetrics and Gynecology Renmin Hospital of Peking UniversityBeijing 100044China Department of Obstetrics and Gynecology Taihe HospitalHubei University of MedicineShiyan 442000China

Objectives:Cisplatin(CDDP)is a widely used and effective basic chemotherapeutic drug for the treatment of a variety of tumors,including ovarian ***,adverse side effects and acquired drug resistance are observed in the clinical application of *** a mode of administration that can alleviate side effects and reduce drug resistance has become a promising strategy to solve this ***:In this study,3 D printing technology was used to prepare a CDDP-poly(lactic-co-glycolic acid)(CDDP-PLGA)polymer compound stent,and its physicochemical properties and cytotoxicity were evaluated both in vitro and in ***:The CDDP-PLGA stent had a significant effect on cell proliferation and apoptosis and clearly decreased the size of subcutaneous tumors in nude mice,whereas the systemic side effects were mild compared with those of intraperitoneal CDDP *** with the control group,CDDP-PLGA significantly increased the mRNA and protein levels of p-glycoprotein(P<0.01;P<0.01)and decreased vascular endothelial growth factor mRNA(P<0.05)and protein levels(P<0.01),however,CDDP-PLGA significantly decreased the mR NA and protein levels of p-glycoprotein(P<0.01;P<0.01)and vascular endothelial growth factor(P<0.01;P<0.01),which are associated with chemoresistance,in subcutaneous tumor *** assay results revealed that,in the CDDP-PLGA group,the staining of the proliferation-related genes Ki67 and PCNA were lightly,and the apoptosis-related gene caspase-3 stained ***:PLGA biomaterials loaded with CDDP,as compared with the same amount of free CDDP,showed good efficacy in terms of cytotoxicity,as evidenced by changes in *** local CDDP release can decrease the systemic side effects of this drug and the occurrence of drug resistance and angiogenesis,and improve the therapeutic *** new approach may be an effective strategy for the local treatment of epithelial ovarian cancer.

关键词： 3D printing drug-loaded stent local treatment cisplatin ovarian cancer

Clinical significance of survivin in the diagnosis and prognosis of endometrial carcinoma

在线全文

学校读者我要写书评

暂无评论

Journal of Nanjing Medical University 2006年第4期20卷 209-213页

作者： yanxiang cheng Gantao Chen Yanjun cheng Demin Pu Department of Obstetrics and Gynecology Tongji HospitalTongji medical collegeHuazhong University of Science and TechnologyWuhan 430030China Department of Medicine of Xiantao Hospital of Hubei Xiantao 433000China

Objective： To investigate the clinical significance of survivin in endometrial carcinoma and to investigate the relationship between the expression of survivin and Ki-67. Methods： Immunohistochemical S-P （streptavidin-biotin-peroxidase complex）method was performed to detect the expression of survivin and Ki-67 antigen in 15 cases of normal endometrium, 21 cases of endometrial simple and complex hyperplasia, 22 cases of endometrial atypical hyperplasia, and 61 cases of endometrial carcinoma. Results： Survivin was hardly detected in some normal endometrium in the proliferative phase and in the secretory phase. However, the level of survivin expression in atypical hyperplasia endometrium（72.73%）was higher than that in normal en- dometrium （7.14%）（P 〈 0.05）, including simple and complex hyperplasia （42.38%）（P 〈 0.01 ）, and was lower than that in endometrial carcinoma（90.17%）（P 〈 0.05）. Moreover, significant correlation was present between the expression of survivin and the characteristics of endometrial carcinoma, including clinical stage, histological grade and the presence of invasion to myometrium （P 〈 0.05）. In addition, Ki-67 antigen expression was positively correlated with survivin expression in all specimen. Ki-67 labeled indexes （LIs）in hyperplasia endometrium were significantly lower than those in atypical hyperplasia endometrium and endometrial carcinoma （P 〈 0.01 ）, while there was no significant difference in Ki-67 LIs between atypical hyperplasia endometrium and endometrial carcinoma（P 〉 0.05）. There was no significant relationship between Ki-67 LIs and the characteristics of endometrial carcinoma, including histological grade, clinical stage or the invasion to myometrium（P 〉 0.05）. Conclusion： Survivin may participate in the onset and progression of endometrial carcinoma through inhibiting apoptosis and promoting proliferation. Survivin expression is correlated with the malignant degree and prognosis of tumor. Ki-67 is also associated with carcinogenesis

关键词： endometrial carcinoma survivin KI-67 immunohistochemistry

RNA interference against hypoxia inducible factor-1α inhibited growth of cervical cancer by limiting vascularization

在线全文

学校读者我要写书评

暂无评论

The Chinese-German Journal of Clinical Oncology 2010年第5期9卷 270-277页

作者： yanxiang cheng Li Hong Xuexian Xu Wen Fan Department of Gynecology and Obstetrics Renmin Hospital of Wuhan University Wuhan 430062 China

Objective:Cervical cancer has become a major public health *** development of effective,systemic therapies for cervical cancer is highly *** show here that hypoxia inducible factor-1α(HIF-1α) was indicated as an attractive therapeutic molecular target for cervical ***:Firstly,we observed the expressional level of HIF-1α in cervical cancer and Hela and Siha cell ***,by constructuring HIF-1α shRNA targeting human HIF-1α mRNA common sequence and transfecting it with plasmid to cervical cell,we detected the changes of HIF-1α and its downstream genes levels *** we injected selected stably transfected cell line into athymic nude mice to estimate its' antitumor ***:We observed that HIF-1α inhibition was related to down-regulated VEGF resulting in prevention of angiogenesis,then leading to slower-growing ***:The underlying concept of transfecting a HIF-1α shRNA expression vector to block the HIF-1α holds promise as the clinical potential of gene therapy for cervical cancer.

关键词： cervical cancer siRNA HIF-1α VEGF gene therapy

Touchable cell biophysics property recognition platforms enable multifunctional blood smart health care

在线全文

学校读者我要写书评

暂无评论

Microsystems & Nanoengineering 2021年第6期7卷 207-219页

作者： Longfei Chen Yantong Liu Hongshan Xu Linlu Ma Yifan Wang Le Yu Fang Wang Jiaomeng Zhu Xuejia Hu Kezhen Yi Yi Yang Hui Shen Fuling Zhou Xiaoqi Gao yanxiang cheng Long Bai Yongwei Duan Fubing Wang Yimin Zhu Affiliations School of Physics&Technology Key Laboratory of Artificial Micro/Nano Structure of Ministry of EducationWuhan UniversityWuhan 430072China Shenzhen Research Institute Wuhan UniversityShenzhen 518000China Department of Hematology Zhongnan HospitalWuhan UniversityWuhan 430071China Department of Laboratory Medicine Zhongnan HospitalWuhan UniversityWuhan 430071China Remin Hospital of Wuhan University Wuhan UniversityWuhan 430060China School of Medicine Zhejiang UniversityHangzhouZhejiang 310002China

As a crucial biophysical property,red blood cell(RBC)deformability is pathologically altered in numerous disease states,and biochemical and structural changes occur over time in stored samples of otherwise normal ***,there is still a gap in applying it further to point-of-care blood devices due to the large external equipment(high-resolution microscope and microfluidic pump),associated operational difficulties,and professional ***,we revolutionarily propose a smart optofluidic system to provide a differential diagnosis for blood testing via precise cell biophysics property recognition both mechanically and *** of the RBC population is caused by pressing the hydrogel via an integrated mechanical transfer *** biophysical properties of the cell population are obtained by the designed smartphone *** intelligence-based modeling of cell biophysics properties related to blood diseases and quality was developed for online *** currently achieve 100%diagnostic accuracy for five typical clinical blood diseases(90 megaloblastic anemia,78 myelofibrosis,84 iron deficiency anemia,48 thrombotic thrombocytopenic purpura,and 48 thalassemias)via real-world prospective implementation;furthermore,personalized blood quality(for transfusion in cardiac surgery)monitoring is achieved with an accuracy of 96.9%.This work suggests a potential basis for next-generation blood smart health care devices.

关键词： smart property enable

Mechanism of anti-tumor effect of HIF-1α silencing on cervical cancer in nude mice

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Chinese-German Journal of Clinical Oncology 2010年第7期9卷 416-421页

作者： yanxiang cheng Wen Fan Bingshu Li Qin Wang Min Hu Li Hong Department of Gynecology Renmin Hospital of Wuhan University Wuhan 430060 China

Objective： The aim of our study was to observe the anti-tumor effect of silencing the expression of HIF-1α on cervical cancer in nude mice and to explore its mechanism of action. Methods： Human cervical cancer cell line Siha cells were divided into 3 groups： mock control group, control group transfected with scrambled sequence plasmid, and experimental group transfected with pU-HIF-la-shRNA eukaryotic expression plasmid. Cultured cells of the three groups were inoculated in nude mice to establish cervical cancer-bearing nude mice. HIF-la RNAi assay was performed to evaluate the tumor-suppressive effect of HIF-1α silencing on cervical cancer-bearing nude mice. Immunohistochemistry and Western blot were used to observe the distribution and protein expression of HIF-1α and GLUT1, while RT-PCR was adopted to detect the gene expression of HIF-1α, GLUTI and HK I1. The product of glycolysis （tactic acid） and apoptosis in tumor cells were detected by colorimetry and semi-quantitative TUNEL staining, respectively. Results： The tumor growth in experimental group was significantly slower than that in the two control groups （P 〈 0.05）. In the 50th day after transplantation, the tumor weight in the experimental group was （1.90 ± 0.28） g, significantly lower than （2.95 ± 0.77） g in the control group and （2.54 ± 0.56） g in the mock group （P 〈 0.01）. In the experimental group, the gene and protein levels of HIF-1α were 0.45 ± 0.04 and 1.25 ± 0.92, and the levels of GLUT1 were 0.32 ± 0.02 and 1.25 ± 0.48, respectively. Both indicators in HIF-la and GLUT1 were lower than that in the two control groups （P 〈 0.05）. The expression levels of HK Ⅱ gene and lactic acid in the experimental group were lower than that in the two control groups （P 〈 0.05）, but the apoptotic cells were much more numerous in the experimental group than that in matched control groups （P 〈 0.01）. Conclusion： The 9ene therapy by siRNAtargeted silencing of HIF-1α may down-regulate its downstream genes GLUT1 and HK Ⅱ

关键词： cervical neoplasms HIF-1α GLUTI RNAi technique nude mice

BLUE LIGHTING-EMITTING POLY（ARYL ETHER）S OF DOPANT/HOST EMITTER SYSTEM

在线全文

学校读者我要写书评

暂无评论

BLUE LIGHTING-EMITTING POLY（ARYL ETHER）S OF DOPANT/HOST EM...