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The RNase III enzyme Dicer1 is essential for larval development in Bombyx mori

Insect Science 2023年第5期30卷 1309-1324页

作者： Dongbin Chen Xu Yang Dehong Yang Yujia liu Yaohui Wang Xingyu Luo Linmeng Tang Meiyan Yi Yongping Huang yanqun liu Zulian liu Department of Sericulture College of Bioscience and BiotechnologyShenyang Agricultural UniversityShenyangChina Key Laboratory of Insect Developmental and Evolutionary Biology Center for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology and EcologyChinese Academy of SciencesShanghaiChina University of Chinese Academy of Sciences BeijingChina Guangzhou Key Laboratory of Insect Development Regulation and Application Research Institute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouChina

MicroRNAs(miRNAs)are important regulators of nearly all aspects of biological processes in *** the biogenesis of miRNAs,the RNase III enzyme Dicer processes double-strand precursor miRNAs into mature miRNAs and promotes the assembly of RNA-induced silencing complexes(RISCs).Dicer has been reported to participate in a wide range of physiological processes,including development and immunity,in some insect ***,the physiological roles of Dicer in lepidopterans remain poorly *** this study,we investigated the function of Bombyx mori *** first performed sequence alignment and found that the sequence of functional domains of Dicer1 are varied among Lepidoptera,Diptera,Coleoptera,Blattaria,and *** a binary clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein 9 genome editing approach,we showed that BmDicer1 mutants have arrested development from the 3rd instar into the 4th *** sequencing analysis indicated that the defects in BmDicer1 mutants are due to dysregulation of genes that encode proteins involved in metabolism,protein degradation,absorption,and renin–angiotensin *** using quantitative real-time polymerase chain reaction showed that mutation of BmDicer1 altered expression of miRNAs and their target ***,our study demonstrates the critical roles of BmDicer1 in miRNA biogenesis and larval development in silkworm.

关键词： Bombyx mori CRISPR/Cas9 Dicer1 larval development

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Design of a Patch Filtering Antenna

Design of a Patch Filtering Antenna

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2019年全国天线年会

作者： liu yanqun Southeast University

In this paper,a patch filtering antenna is analyzed and designed using characteristic mode analysis（CMA）.Two pairs of slots are respectively ecthed on the patch and GND plate of the patch antenna,which is used to adjust the modal weighting coefficient（MWC） of the Characteristic Modes（CMs） so as to obtain the gain *** the gain passband,the desired mode（broadside radiation） is well excited,while in the transition band,undesired mode（non-broadside mode） are adjusted so that its excitation degree exceeds that of desired *** is used to indicate the excitation degree of each CM,thus guiding the filtering antenna *** optimizing the dimension of the additional slots,a patch filtering antenna with good performance is finally designed, and the gain zero at the transition band is 20 dB lower than the passband gain at least.

关键词： filtering antenna modal weighting coefficient

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Phosphorus Recovery as Struvite from the Supernatant of Anaerobic Digestion in Wastewater Treatment Plant

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Journal of Environmental Science and Engineering 2010年第1期4卷 14-19页

作者： Gang Xue Yanan liu yanqun Wang School of Environmental Science and Engineering Donghua University Shanghai 201620 China

Phosphorus is an irreplaceable and depletable element. Furthermore, it has an almost one-way circulation on earth, so it is necessary to close the phosphorus cycle loop. Phosphorus could be recovered as struvite, which is a good slow-released fertilizer for agriculture. The supernatant of anaerobic digestion used to treat sludge from wastewater treatment plant is one main source from which phosphorus can be recovered. Studies have proven that phosphorus recovery from digester supematant is a feasible choice to preserve phosphorus rock technically and economically. A modified ＂PHOSNIX＂ P-recovery process was applied under the operating conditions of a 9.0 pH value and a 1.8 mg： P ratio with the influent of the centrate coming from the sludge centrifuge of the Songjiang Wastewater Treatment Plant in Shanghai. More than 80% influent phosphorus was recovered as struvite. Crystal products with good purity and low heavy metal content were gained. The largest crystal had a length of up to 0.26 mm. It was found in our study that the reaction time did not play an important role in crystal growth. Therefore, the optimization of the reaction condition for crystal growth should be examined in future study.

关键词： Phosphorus recovery struvite supernatant anaerobic digestion wastewater treatment plant.

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A Modified Centered Climbing Algorithm for Linear Programming

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Applied Mathematics 2012年第10期3卷 1423-1429页

作者： Ming-Fang Ding yanqun liu John Anthony Gear School of Mathematical & Geospatial Sciences RMIT University Melbourne Australia

In this paper, we propose a modified centered climbing algorithm (MCCA) for linear programs, which improves the centered climbing algorithm (CCA) developed for linear programs recently. MCCA implements a specific climbing scheme where a violated constraint is probed by means of the centered vector used by CCA. Computational comparison is made with CCA and the simplex method. Numerical tests show that, on average CPU time, MCCA runs faster than both CCA and the simplex method in terms of tested problems. In addition, a simple initialization technique is introduced.

关键词： Linear Programming Ladder Method Climbing Rules Simplex Method

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The complete chloroplast genome of the sawtooth oak, Quercus acutissima and its intraspecific polymorphism

The complete chloroplast genome of the sawtooth oak, Quercus...

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中国蚕学会2018年学术年会

作者： HuaLei Hu Jian Yang yanqun liu Li Qin Department of Sericulture College of Bioscience and Biotechnology

The sawtooth oak, Quercus acutissima Carruth, is an Asian species of oak and widely distributed throughout China. This species is an feed plant that oak silkworm prefers to eat. Here, we report the complete chloroplast（cp） genome of this species. The leaves were sampled from a 40-year-old tree that suffers short rotation every four years to rear oak silkworm larvae at the silkworm field of Shenyang Agricultural University. The genome is 161,115 bp in size with a GC content of 36.83% and contains 134 genes（86 protein-coding genes, 40 tR NA genes, eight rR NA genes）. The large single-copy region and small single-copy region in the cp genome contain 90,455 bp and 19,096 bp, respectively, while the inverted repeat region is 25,798 bp in length. The phylogenetic analyses based on the whole genome sequence provided a solid evidence that Q. acutissima has a close relationship with Q. variabilis. Comparative genomics revealed 267 variable sites including 23 transition, 38 transversion and 206 insertion/deletion between the cp genome we presented here and the recently published cp genome sampled from Zijin Mountain in Nanjing, China, indicating the presence of intraspecific polymorphism within the genomes of Q. acutissima individuals. The Q. acutissima cp genome containing intraspecific polymorphism will be essential for studying population genetics as well as meeting management goals.

关键词： Quercus acutissima chloroplast genome phylogenetic position intraspecific polymorphism

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The Application Prospect Autologous Bone Marrow Extract Buffy Coat in Cartilage Tissue Engineering: A Mini-Review

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Journal of Biosciences and Medicines 2019年第12期7卷 9-17页

作者： Zhiyu Li Zhongye Zhang Guo Song Shouyong Fu Longhao Jin yanqun liu Department of Orthopedic Surgery Yanbian University Hospital Yanji China Department of Orthopedic Surgery Songyuan Central Hospital Songyuan China

Cartilage injury is an important cause of osteoarthritis;however, there is no effective method of cartilage regeneration at present. Therefore, it would be of great value to explore and develop new methods of cartilage regeneration. In recent years, it has become a hot topic on repairing damaged cartilage by using tissue engineered cartilage domestic and abroad. It’s the key factor to success for getting cell source which is effective, applicable and easy to obtain in the technology of cartilage tissue engineering filed. This article summarizes the research progress of bone marrow extract buffy coat in cartilage tissue engineering.

关键词： Buffy Coat Cartilage Tissue Engineering

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模式可配置的NAND Flash纠错系统设计与实现

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中南大学学报（自然科学版） 2013年第5期44卷 1918-1925页

作者：徐富新刘应刘雁群向超中南大学物理与电子学院湖南长沙410083

针对NAND flash存储器设计一种模式可配置的纠错系统的电路结构,该结构可以预防错误位数大于设计纠错位数的情况发生。提出一种高速并行BCH编译码的电路设计方法,并导出一种无需有限域求逆运算的BM迭代算法的硬件实现方法。通过复用编... 详细信息

针对NAND flash存储器设计一种模式可配置的纠错系统的电路结构,该结构可以预防错误位数大于设计纠错位数的情况发生。提出一种高速并行BCH编译码的电路设计方法,并导出一种无需有限域求逆运算的BM迭代算法的硬件实现方法。通过复用编码算法电路与译码算法电路,同时结合流水线技术与乒乓操作技术,实现以较小的硬件资源开销提高纠错系统性能。该纠错系统电路在EP4CE15E22C8系列FPGA芯片上实现,并进行测试分析。测试结果表明:在相同的系统工作频率下,该纠错系统电路的数据吞吐率是传统串行纠错电路的8倍,而硬件资源开销只增加1倍;与传统的NAND flash纠错电路相比,该纠错电路结构相对独立,可移植性强,可满足多种应用场合的需要。

关键词： Nand flash存储器纠错电路可配置模式 BCH码现场可编程门阵列

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Comparison of Chondrogenic Ability between Mesenchymal Stem Cells and Buffy Coat <i>in Vitro</i>

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Journal of Biosciences and Medicines 2018年第3期6卷 86-94页

作者： Longhao Jin Zhongye Zhang yanqun liu Kumar Gurung Yongjun Jin Yuanming He Yu Jin Zhehu Jin Department of Orthopedic Surgery Yanbian University Hospital Yanji City China Department of Orthopedic Surgery Songyuan Central Hospital Songyuan City China Department of Anatomy School of Medicine Yanbian University Yanji City China Department of Dermatology Yanbian University Hospital Yanji City China

Objective: In vitro comparison of chondrogenic differentiation ability of bone marrow extracted mesenchymal stem cells (MSCs) and buffy coat. Methods: MSCs of New Zealand white rabbits were cultured in vitro and adherent cells were passaged. The cells were inoculated on polyglycolic acid (PGA) scaffold (3 mm in diameter and 2 mm in height) liquid for 21 days. Under the same conditions, without subculture, buffy coat was directly inoculated into the cell scaffold. The degree of chondrogenic differentiation was compared by Safranin-Ostaining, histological scoring and biochemical functional detection. Results: The chondrogenic differentiation ability of the buffy coat group was better than that of the MSC group. Safranin-Ostain was stronger in buffy coat group than in MSC group. The Bern Score was also higher in the buffy coat group than in the MSC group and the total amount of sulfated glycosaminoglycans (GAGs) in buffy coat group was higher than that in MSC group. Conclusion: The chondrogenic differentiation ability of buffy coat is higher than that of MSC. Through this result, it was found buffy coat can also differentiate into sub-cartilage without stimulating growth factor. Thus, buffy coat can make a great source in artificial cartilage engineering.

关键词： Chondrogenic Buffy Coat Mesenchymal Stem Cells

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Molecular evidence for a Northern China origin of the domesticated silkworm, rather than a Southern China

Molecular evidence for a Northern China origin of the domest...

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中国蚕学会2018年学术年会

作者： DongBin Chen RuSong Zhang RunXi Xia YuPing Li yanqun liu Cheng Lu Department of Sericulture College of Bioscience and Biotechnology State Key Laboratory of Silkworm Genome Biology Southwest University

The domesticated silkworm Bombyx mori is the only truly domesticated insect, and has connected with human for ～5500 years. However, the geographic origin of the domesticated silkworm remains to be established. To address this question, we analyzed the mitochondrial genomes of 39 domesticated silkworms representing all major domesticated silkworm population worldwide and 16 wild silkworms available. First, we identify two genetically distinctive subtypes in Chinese wild silkworms, corresponding to Northern Chinese B. mandarina and Southern Chinese B. mandarina, respectively. Most important, we show a clear molecular evidence that the true wild progenitor of the domesticated silkworm is Northern Chinese B. mandarina, rather than Southern Chinese B. mandarina, implying that the early domestication event may have occurred in Northern China. The molecular evidence is consistent with the widespread legend that suggests the domesticated silkworm was first domesticated in the lower and middle reaches of the yellow river.

关键词： Bombyx mori Bombyx mandarina Wild ancestor Geographic origin

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家蚕的起源与进化研究进展

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蚕业科学 2018年第3期44卷 353-358页

作者：刘彦群鲁成沈阳农业大学生物科学技术学院沈阳110866 西南大学蚕学与系统生物学研究所重庆400715

家蚕（Bombyx mori）的驯化是农业历史上的重大事件之一,中国的养蚕业已有近7 000年的历史。关于家蚕的起源与进化不仅是蚕丝文化和蚕业发展历史溯源研究的焦点,也一直是蚕学界关注的基础科学问题,研究的核心是家蚕的直接野生祖先来源... 详细信息

家蚕（Bombyx mori）的驯化是农业历史上的重大事件之一,中国的养蚕业已有近7 000年的历史。关于家蚕的起源与进化不仅是蚕丝文化和蚕业发展历史溯源研究的焦点,也一直是蚕学界关注的基础科学问题,研究的核心是家蚕的直接野生祖先来源和起源地（驯化事件的发生地）。家蚕的野生祖先是野桑蚕（Bombyx mandarina）为学界早期的共识。近20多年又从染色体水平和DNA序列水平进一步证实了家蚕的野生祖先是中国野桑蚕,而不是日本野桑蚕,家蚕起源于中国野桑蚕的观点在学界也达成了共识。但是,家蚕的起源地仍存在单起源中心（在中国北方的某个地区驯化而来）和多起源中心（在华北、华东、华南,以及印度和越南等不同地区独立驯化而来）的争论,澄源正本尚缺乏遗传学证据。本文综述应用分子生物学技术研究家蚕起源与进化的重要进展,根据动物起源与进化研究的最新方法,提出了基于线粒体基因组扫描和大样本策略的家蚕起源与进化研究设想。

关键词：家蚕野桑蚕野生祖先起源地线粒体基因组

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