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High-resolution polarization-sensitive optical coherence tomography and optical coherence tomography angiography for zebrafish skin imaging

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Journal of Innovative Optical Health Sciences 2021年第6期4卷 131-144页

作者： Di Yang Zhuoqun Yuan Zihan Yang Muyun Hu yanmei liang Institute of Modern Optics Nankai University Tianjin Key Laboratory of Micro-scale Optical Information Science and TechnologyTianjin 300350P.R.China

Zebrafish is an important animal model,which is used to study development,pathology,and genetic *** zebrafish skin model is widely used in cutaneous research,and angiogenesis is critical for cutaneous wound ***,limited by the penetration depth,the available optical methods are difficult to describe the internal skin structure and the connection of blood vessels between the skin and subcutaneous *** a homemade high-resolution polarization-sensitive optical coherence tomography(PS-OCT)system,we imaged the polarization contrast of zebrafish skin and the zebrafish skin vasculature with optical coherence tomography angiography(OCTA).Based on these OCT images,the spatial distribution of the zebrafish skin vasculature was ***,we monitored the healing process of zebrafish cutaneous *** think the high-resolution PS-OCT system will be a promising tool in studying cutaneous models of zebrafish.

关键词： Polarization-sensitive optical coherence tomography optical coherence tomography angiography zebrafish skin vasculature

Identification of oral squamous cell carcinoma in optical coherence tomography images based on texture features

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Journal of Innovative Optical Health Sciences 2021年第1期4卷 18-27页

作者： Zihan Yang Jianwei Shang Chenlu Liu Jun Zhang yanmei liang Institute of Modern Optics Nankai University Tianjin Key Laboratory of Micro-Scale Optical Information Science and TechnologyTianjin 300350P.R.China Department of Oral Pathology Tianjin Stomatological Hospital Hospital of Stomatology Nankai UniversityTianjin 300041P.R.China Department of Oral Medicine Tianjin Stomatological Hospital Hospital of Stomatology Nankai UniversityTianjin 300041P.R.China Department of Oral-Maxillofacial Surgery Tianjin Stomatological Hospital Hospital of Stomatology Nankai UniversityTianjin 300041P.R.China

Surgical excision is an effective treatment for oral squamous cell carcinoma(OSCC),but exact intraoperative differentiation OSCC from the normal tissue is the first *** a noninvasive imaging technique,optical coherence tomography(OCT)has the nearly same resolution as the histopathological examination,whose images contain rich information to assist surgeons to make clinical *** extracted kinds of texture features from OCT images obtained by a home-made swept-source OCT system in this paper,and studied the identification of OSCC based on different combinations of texture features and machine learning *** was demonstrated that different combinations had different accuracies,among which the combination of texture features,gray level co-occurrence matrix(GLCM),Laws'texture measnres(LM),and center symmetric auto-correlation(CSAC),and SVM as the classifier,had the optimal comprehensive identification effect,whose accuracy was 94.1%.It was proven that it is feasible to distinguish OSCC based on texture features in OCT images,and it has great potential in helping surgeons make rapid and accurate decisions in oral clinical practice.

关键词： Optical coherence tomography oral squamous cell carcinoma identification texture features machine learning

Intraoperative imaging of oral-maxillofacial lesions using optical coherence tomography

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Journal of Innovative Optical Health Sciences 2020年第2期13卷 93-103页

作者： Zihan Yang Jianwei Shang Chenlu Liu Jun Zhang Fang Hou yanmei liang Institute of Modern Optics Nankai University NankaiTianjin 300350P.R.China Department of Oral Pathology Tianjin Stomatological Hospital Hospital of Stomatology of Nankai University NankaiTianjin 300041P.R.China Department of Oral Medicine Tianjin Stomatological Hospital Hospital of Stomatology of Nankai University NankaiTianjin 300041P.R.China Department of Oral-Maxillofacial Surgery Tianjin Stomatological Hospital Hospital of Stomatology of Nankai University Nankai Tianjin 300041P.R.China

Histopathological examination is still the gold standard for diagnoses of oral-maxillofacial lesions,but it is invasive and *** coherence tomography(OCT)provides a kind of noninvasive,label-free,real-time and high-resolution imaging *** this study,in order to assess the feasibility of OCT in oral clinical application,fresh excised tissue specimens from 59 patients undergoing oral-maxillofacial surgery were imaged in detail by using a benchtop sweptsource OCT *** is shown that different lesions or tissues can be obviously distinguished based on their different microstructural features in OCT images,and the features are similar to those of their corresponding histopathological *** is proven that OCT has great feasibility and potential as a diagnostic aid for surgeons in oral medicine.

关键词： Oral-maxillofacial lesions optical coherence tomography intraoperative imaging feasibility assessment

Applications of scanning femtosecond laser-induced ionization microscopy in biological imaging

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Frontiers of Optoelectronics 2008年第Z2期1卷 201-204页

作者： Youbo ZHAO yanmei liang Xiaonong ZHU Institute of Modern Optics Nankai University Key Laboratory of Opto-electronic Information Science and Technology Ministry of Education of China

The applicability and potential advantages of femtosecond laser-induced ionization microscopy in examining biological samples are demonstrated. Highresolution section images of the inner structures and the topographical surface profiles of a piece of unstained onion skin and a human oral epithelium specimen are obtained by collecting the plasma emission signals generated either inside the samples or from air in the vicinity. It is concluded that the high material discrimination capability and the minimum damage effect give this laser-induced ionization imaging technique great potentials in the area of biological imaging and medical analyses.

关键词： nonlinear microscopy laser-tissue interaction ultrafast laser bio-imaging

Analyses and calculations of noise in optical coherence tomography systems

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Frontiers of Optoelectronics 2008年第Z2期1卷 247-257页

作者： Xiaonong ZHU yanmei liang Youxin MAO Yaqing JIA Yiheng LIU Guoguang MU Institute of Modern Optics Nankai University Key Laboratory of Opto-electronic Information Science and Technology Ministry of Education Institute for Microstructural Science National Research Council Canada

Significant progress has been made in the study of optical coherence tomography(OCT) — a non-invasive, high resolution, and in vivo diagnostic method for medical imaging applications. In this paper, the principles of noise analyses for OCT systems have been *** are made of signal-to-noise ratios for both balanced and unbalanced detection schemes under the ideal no-stray light situation as well as the non-ideal situation where residual reflections and scatterings are presented. Numerical examples of noise calculation accompanied by detailed comparison of the main characteristics of both time-domain and frequency-domain OCT systems are also presented. It is shown that a larger dynamic range can be achieved for a Fourier-domain OCT system even under the circumstances of high-speed image *** main results presented in this paper should be useful for the development of high performance OCT systems.

关键词： optical coherence tomography (OCT) noise analyses sensitivity signal-to-noise ratio (SNR)

Deep learning-based inpainting of saturation artifacts in optical coherence tomography images

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Journal of Innovative Optical Health Sciences 2024年第3期17卷 1-10页

作者： Muyun Hu Zhuoqun Yuan Di Yang Jingzhu Zhao yanmei liang Institute of Modern Optics Nankai UniversityTianjin Key Laboratory of Micro-scale Optical Information Science and TechnologyTianjin 300350China Department of Thyroid and Neck Tumor Tianjin Medical University Cancer Institute and Hospital National Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjin 300060China

Limited by the dynamic range of the detector,saturation artifacts usually occur in optical coherence tomography(OCT)imaging for high scattering *** available methods are difficult to remove saturation artifacts and restore texture completely in OCT *** proposed a deep learning-based inpainting method of saturation artifacts in this *** generation mechanism of saturation artifacts was analyzed,and experimental and simulated datasets were built based on the *** super-resolution generative adversarial networks were trained by the clear–saturated phantom image *** perfect reconstructed results of experimental zebrafish and thyroid OCT images proved its feasibility,strong generalization,and robustness.

关键词： Optical coherence tomography saturation artifacts deep learning image inpainting.

Identification of pathological characteristics in pulmonary tuberculosis using polarization-sensitive optical coherence tomography

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Journal of Innovative Optical Health Sciences 2024年第6期17卷 97-106页

作者： Yun Ding Zhuoqun Yuan Xiaojiang Zhao Guozheng Gao Xin Li yanmei liang Clinical School of Thoracic Tianjin Medical University TianjinP.R.China Department of Thoracic Surgery Fujian Provincial Hospital FujianP.R.China Institute of Modern Optics Nankai University Tianjin Key Laboratory of Micro-scale Optical Information Science and Technology Tianjin 300350P.R.China School of Medicine Tianjin University TianjinP.R.China Department of Pathology Tianjin Chest Hospital No.261Taierzhuang South Road Jinnan District Tianjin 300222P.R.China Department of Thoracic Surgery Tianjin Chest Hospital TianjinP.R.China

The low detection rate of Mycobacterium tuberculosis in clinical practice leads to a high rate of missed diagnosis for pulmonary tuberculosis(PTB).As a noninvasive,high-resolution,real-time imaging technology,polarization-sensitive optical coherence tomography(PS-OCT)may be feasible for the rapid identification of pathological *** study aimed to explore the feasibility of using PS-OCT to identify pathological features of *** the experiments,PTB samples containing some surrounding lung tissues were imaged using *** is demonstrated that PS-OCT images showed good consistency with the corresponding pathological images and were able to identify PTB-related characteristic pathological *** think PS-OCT can serve as an effective supplement for the diagnosis of PTB,enabling rapid and accurate diagnosis,and aiding in the understanding of the pathological characteristics and pathophysiological processes of PTB.

关键词： Pulmonary tuberculosis PS-OCT pathological features.

Shape description matrix and its applications to color-image retrieval and recognition

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Science China(Technological Sciences) 2004年第2期47卷 159-165页

作者： WANG Yi,ZHAI Hongchen,liang yanmei,ZHANG Siyuan & MU Guoguang Institute of Modern Optics,Nankai University,Tianjin 300071,China Key Laboratory of Information Sci-ence and Technology MOE,Tianjin 300071,China Institute of Modern Optics Nankai University Tianjin China Key Laboratory of Information Science and Technology MOE Tianjin China

Shape description matrix (SDM) describing regional pixel distribution characteristics of images is defined, with both radial and angular distribution messages taken into account to offer a comprehensive and exact description of the shape characteristics even upon a blurred edge. Using this SDM, a general color histogram (GCH) is developed. Both theoretical and experimental results show that the GCH can be applied to color-image retrievals and recognitions with a high efficiency.

关键词： SDM, similarity degree, match degree, general color histogram.

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基于三维卷积神经网络的彩色傅里叶叠层显微术

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光学学报 2020年第20期40卷 92-99页

作者：张慕阳梁艳梅南开大学现代光学研究所天津300350

包含多波长信息的低分辨(LR)灰度图难以被完全解复用,根据LR图像信息重建出的彩色高分辨(HR)图像容易出现通道串扰的现象。为重建不受通道串扰干扰的彩色HR图像,提出一种基于三维卷积神经网络(CNN)的彩色HR图像重建算法。采用主成分分... 详细信息

包含多波长信息的低分辨(LR)灰度图难以被完全解复用,根据LR图像信息重建出的彩色高分辨(HR)图像容易出现通道串扰的现象。为重建不受通道串扰干扰的彩色HR图像,提出一种基于三维卷积神经网络(CNN)的彩色HR图像重建算法。采用主成分分析法提取单色HR图像和彩色LR图像的结构信息,然后基于结构信息训练CNN来建立单色HR图像和彩色LR图像之间的映射关系,最后生成彩色HR图像。实验结果表明,所提算法可以获得不受通道串扰影响、色彩不失真的彩色HR图像。定量评价指标方均根误差小于0.1,结构相似性参数大于0.9。

关键词：成像系统显微术傅里叶叠层显微成像卷积神经网络主成分分析彩色图像重建

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