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The interaction of influenza H5N1 viral hemagglutinin with sialic acid receptors leads to the activation of human γδT cells

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Cellular & Molecular Immunology 2013年第6期10卷 463-470页

作者： yanlai lu Zhen Li Chi Ma Hao Wang, Jing Zheng Lianxian Cui Wei He Department of Immunology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences and School of Basic Medicine Peking UnionMedical College State Key Laboratory of Medical Molecular Biology Beijing China

Highly pathogenic avian influenza H5N 1 epidemics are a significant public health hazard. Genetically engineered H5N 1 viruses with mammalian transmission activity highlight the potential risk of a human influenza H5N 1 pandemic. Understanding the underlying principles of the innate immune system in response to influenza H5N 1 viruses will lead to improved prevention and control of these potentially deadly viruses, γδT cells act as the first line of defense against microbial infection and help initiate adaptive immune responses during the early stages of viral infection. In this study, we investigated the molecular mechanisms of γδ T cells in response to influenza H5N1 viral infection, We found that recombinant hemagglutinin （rHA） derived from three different strains of influenza H5N 1 viruses elicited the activation of γδ T cells cultured in peripheral blood mononuclear cells （PBMCs）. Both the cell surface expression of CD69, an early activation marker on γδ T cells, and the production of interferon-y （IFN-y） were significantly increased. Notably, the rHA protein-induced γδ T-cell activation was not mediated by TCRγδ, NKG2D or pattern recognition receptors （PRRs） or NKp46 receptors. The interaction of rHA proteins with sialic acid receptors may play a critical role in γδ T-cell activation. Our data may provide insight into the mechanisms underlyingγδT-cell activation in response to infection with H5N1 viruses.

关键词： γδ T cells hemagglutinin highly pathogenic avian influenza H5N1 virus

Author Correction:A novel recombinant DNA vaccine encoding Mycobacterium tuberculosis ESAT-6 and FL protects against Mycobacterium tuberculosis challenge in mice

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The Journal of Biomedical Research 2022年第6期36卷 448-450页

作者： Qingtao Jiang Jing Zhang Xia Chen Mei Xia yanlai lu Wen Qiu Ganzhu Feng Dan Zhao Yan Li Fengxia He Guangyong Peng Yingwei Wang Department of Microbiology and Immunology Nanjing Medical UniversityNanjingJiangsu 211166China Division of Infectious Diseases Allergy and ImmunologyDepartment of Internal MedicineSaint Louis University School of MedicineSt.LouisMissouri 63104USA

Correction to:Journal of Biomedical Research https://***/10.7555/JBR.27.20120114,published on 30 September *** apologize for the misused images in Fig.7 in our article published on 30 September *** Fig.7 by HE staining and immunohistochemistry were partially misused because of our *** we couldn't find the original pictures acquired 11 years ago,thus HE and IHC experiments were repeated by our previously reserved wax ***,the pictures taken by microscope imaging system in each group were collected and combined,*** correct version of Fig.

关键词： tuberculosis immunohistochemistry picture

Generation of human neutralizing monoclonal antibodies against the 2009 pandemic H 1N 1 virus from peripheral blood memory B lymphocytes

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Cellular & Molecular Immunology 2013年第5期10卷 403-412页

作者： Hao Wang Chi Ma yanlai lu Xu Ji Yongsheng Pang Fang Hua Lianxian Cui Denian Ba Wei He Department of Immunology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences and School of Basic Medicine Peking UnionMedical College State Key Laboratory of Medical Molecular Biology Beijing China

The 2009 H 1N 1 influenza pandemic demonstrated the significance of a global health threat to human beings. Although pandemic H 1N 1 vaccines have been rapidly developed, passive serotherapy may offer superior immediate protection against infections in children, the elderly and immune-compromised patients during an influenza pandemic. Here, we applied a novel strategy based on Epstein-Barr virus （EBV）-immortalized peripheral blood memory B cells to screen high viral neutralizing monoclonal antibodies （MAbs） from individuals vaccinated with the 2009 pandemic H1N1 vaccine PANFlu.1. Through a massive screen of 13 090 immortalized memory B-cell clones from three selected vaccinees, seven MAbs were identified with both high viral neutralizing capacities and hemagglutination inhibition （HAl） activities against the 2009 pandemic H 1N 1 viruses. These MAbs may have important clinical implications for passive serotherapy treatments of infected patients with severe respiratory syndrome, especially children, the elderly and immunodeficient individuals. Our successful strategy for generating high-affinity MAbs from EBV-immortalized peripheral blood memory B ceils may also be applicable to other infectious or autoimmune diseases.

关键词： Epstein-Barr virus hemagglutinin memory B cells neutralizing monoclonal antibody 2009 pandemicinfluenza HIN1 virus

Sublytic C5b-9 triggers glomerular mesangial cell apoptosis in rat Thy-1 nephritis via Gadd45 activation mediated by Egr-1 and p300-dependent ATF3 acetylation

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Journal of Molecular Cell Biology 2016年第6期8卷 477-491页

作者： Fengxia He Mengya Zhou Tianyi Yu Dan Zhao Jing Zhang Wen Qiu yanlai lu Yu Liu lulu Wang Yingwei Wang Department of Immunology Nanjing Medical UniversityNanjing 210029China

The apoptosis of glomerular mesangial cells(GMCs)is considered to be an important contributor to the initiation and development of rat Thy-1 nephritis(Thy-1N)and is accompanied by sublytic C5b-9 ***,the mechanism by which sublytic C5b-9 triggers GMC apoptosis has not been *** this study,functional and histological examinations were performed on GMCs treated with sublytic C5b-9(in vitro)and renal tissues of Thy-1N rats(in vivo).The in vitro studies found that sublytic C5b-9 could trigger GMC apoptosis through upregulating Egr-1,ATF3,and Gadd45 ***-1-mediated post-transcriptional modulation of ATF3,Egr-1/ATF3-enhanced Gadd45 promoter activity,and p300-mediated ATF3 acetylation were all involved in GMC *** importantly,the effective binding elements for Egr-1 and ATF3 to Gadd45β/γpromoters and the ATF3 acetylation site were *** vivo,silencing renal p300,Egr-1,ATF3,and Gadd45β/γsignificantly decreased GMC apoptosis,secondary GMC proliferation,and urinary protein secretion in Thy-1N ***,these findings implicate that sublytic C5b-9-induced activation of Egr-1/p300–ATF3/Gadd45 axis plays a critical role in GMC apoptosis in Thy-1N rats.

关键词： glomerular mesangial cells sublytic C5b-9 Thy-1 nephritis transcription factor acetylation apoptosis

A novel recombinant DNA vaccine encodingMycobacterium tuberculosis ESAT-6 and FL protects againstMycobacterium tuberculosis challenge in mice

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The Journal of Biomedical Research 2013年第5期27卷 406-420页

作者： Qingtao Jiang Jing Zhang Xia Chen Mei Xia yanlai lu Wen Qiu Ganzhu Feng Dan Zhao Yan Li Fengxia He Guangyong Peng Yingwei Wang Department of Microbiology and Immunology Nanjing Medical University Division of Infectious Diseases Allergy and Immunology Department of Internal MedicineSaint Louis University School of Medicine

Mycobacterium tuberculosis 6-kDa early secretory antigenic target （ESAT-6） is a dominant target antigen for cell-mediated immunity in the early phase of tuberculosis. The fms-like tyrosine kinase 3 ligand （FL） that induces potent immune response has been used as an adjuvant in vaccine development. In this study, a new recombinant plasmid （plRES-epitope-peptides-FL） encoding three T cell epitopes of ESAT-6 and FL was constructed, and the immunogenicity of the DNA vaccine was assessed in C57BL/6 mice immunized with the plasmid DNA vaccine. Additionally, a strategy of intramuscular injection with the DNA vaccine （prime） and intranasal administration of the epitope peptides （boost） was employed to induce higher immune reaction of the mice. The results showed that mice vaccinated with the recombinant plasmid DNA vaccine and boosted with the peptides not only increased the levels of Thl cytokines （IFN-γ and IL-12）, the number of IFN-γ＋ T cells and activities of cytotoxic T lymphocytes as well as IgG, but also enhanced protection against Mycobacterium tuberculosis challenge. In conclusion, these data indicate that the novel recombinant plRES-epitope-peptides-FL plasmid is a useful DNA vaccine for pre- venting Mycobacterium tuberculosis infection.

关键词： early secretory antigenic target-6 (ESAT-6) fms-like tyrosine kinase 3 ligand (FL) Mycobacteriumtuberculosis recombinant plasmid T cell epitopes

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泉山湖花园城规划

建筑学报 2007年第2期 82-83页

作者：高松卢艳来安徽省建筑设计研究院合肥230001

1 尊重地域景观，充分保护自然生态环境尊重地域景观，在充分保护自然生态环境的基础上提升生态环境质量，是贯穿设计全过程的重要原则。

关键词：花园城自然生态环境规划生态环境质量地域景观全过程尊重保护

Sublytic C5b-9 Induces IL-6 and TGF-β1 Production by Glomerular Mesangial Cells Through p300-Mediated C/EBPβ Acetylation

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Sublytic C5b-9 Induces IL-6 and TGF-β1 Production by Glomer...

Sublytic C5b-9 Induces IL-6 and TGF-β1 Production by Glomerular Mesangial Cells through p300-mediated C/EBPβAcetylation

第十六届中国科协年会——分13感染、免疫和疫苗论坛

作者： Jing ZHANG Yan LI Kai SHAN lulu WANG Wen QIU yanlai lu Dan ZHAO Gangqian ZHU Fengxia HE Yingwei WANG Department of Microbiology and Immunology Nanjing Medical University

关键词： transcription factor,post-translational modification,renal inflammation,renal pathology

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Sublytic C5b-9 Induces IL-6 and TGF-β1 Production by Glomer...

Sublytic C5b-9 triggers glomerular mesangial cell apoptosis via XAF1 gene activation mediated by p300-dependent IRF-1 acetylation

第九届全国免疫学学术大会

作者： Jing Zhang Yan Li Kai Shan lulu Wang Wen Qiu yanlai lu Dan Zhao Gangqian Zhu Fengxia He Yingwei Wang Department of Immunology Nanjing Medical University

The increased production of IL-6 and TGF-β1 by glomerular mesangial cells(GMC)in rat Thy-1 nephritis(Thy-1N),a model of human mesangioproliferative glomerulonephritis(MsPGN),is accompanied by sublytic C5b-9 deposition,but the mechanism of IL-6 and TGF-β1 upregulation in rat GMC triggered by sublytic C5b-9 in this model has not been *** the present study,molecular biological methods,along with functional and histological studies were *** found that sublytic C5b-9(here,less than 5%LDH release from cells is regarded as a sublytic effect)could induce the production of IL-6 and TGF-β1 through upregulating p300 and C/EBPB expression in ***,C/EBPB-enhanced IL-6 and TGF-pi gene activation and p300-mediated C/EBPB acetylation were involved in promoter activity of IL-6 and TGF-β1 in GMC attacked by sublytic *** detail,the elements of C/EBPp binding to rat IL-6and TGF-β1 promoter and three acetylated sites of rat C/EBPp protein were first ***,silencing p300or C/EBPβgene in rat kidney significantly reduced the production of IL-6 and TGF-β1 and renal lesions in Thy-1N ***,these data implicate that mechanism of IL-6 and TGF-β1 production in renal tissue of Thy-1N rats is associated with sublytic C5b-9-upregulated p300 and p300-mediated C/EBPβacetylation as well as C/EBPP-activated IL-6 and TGF-β1 genes.

关键词： transcription factor post-translational modification renal inflammation renal pathology

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Sublytic C5b-9 triggers glomerular mesangial cell apoptosis ...