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Heme Oxygenase-1 is Associated with Wheat Salinity Acclimation by Modulating Reactive Oxygen Species Homeostasis

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Journal of Integrative Plant Biology 2011年第8期53卷 653-670页

作者： yanjie xie Weiti Cui Xingxing Yuan Wenbiao Shen Qing Yang College of Life Sciences Cooperative Demonstration Laboratory of Centrifuge Technique Nanjing Agricultural University and Beckman Coulter Ltd. Co. Nanjing Agricultural University Nanjing 210095 China The Institute of Vegetable Crops Jiangsu Academy of Agricultural Sciences Nanjing 210014 China

Heme oxygenase-1 （HO-1） has been recently identified as an endogenous signaling system in animals. In this study, HO-1 upregulation and its role in acquired salt tolerance （salinity acclimation） were investigated in wheat plants. We discovered that pretreatment with a low concentration of NaCl （25 mmol/L） not only led to the induction of HO-1 protein and gene expression, as well as enhanced HO activity, but also to a salinity acclimatory response thereafter. The effect is specific for HO-1, since the potent HO-1 inhibitor zinc protoporphyrin IX blocks the above cytoprotective actions, and the cytotoxic responses conferred by 200 mmol/L NaCl are reversed partially when HO-1 inducer hemin is added. Heme oxygenase catalytic product, carbon monoxide （CO） aqueous solution pretreatment, mimicked the salinity acclimatory responses. Meanwhile, the CO-triggered re-establishment of reactive oxygen species （ROS） homeostasis was mainly guaranteed by the induction of total and isozymatic activities, or corresponding transcripts of superoxide dismutase, ascorbate peroxidase, and cytosolic peroxidase （POD）, as well as the downregulation of NADPH oxidase expression and cell-wall POD activity. A requirement of hydrogen peroxide homeostasis for HO-1-mediated salinity acclimation was also discovered. Taken together, the above results suggest that the upregulation of HO-1 expression was responsible for the observed salinity acclimation through the regulation of ROS homeostasis.

关键词： carbon monoxide haem oxygenase-1 lipid peroxidation ROS homeostasis salinity acclimation wheat.

旱季污水处理厂污水中溶解性有机质特征及其与氮素关系

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环境工程技术学报 2024年第3期14卷 941-952页

作者：刘交潘国强杨帆李晓曼谢彦杰韦敏祥任美洁云南大学国际河流与生态安全研究院昆明城市污水处理运营有限责任公司昆明理工大学环境科学与工程学院

针对市政污水处理厂污水中溶解性有机质(DOM)变化及其与氮素可能存在的相互影响关系,采用三维荧光光谱结合平行因子分析以及相关性分析,以西南某市旱季市政污水处理厂为研究对象,探究DOM荧光组分随工艺单元的变化规律及其与氮素转化的... 详细信息

针对市政污水处理厂污水中溶解性有机质(DOM)变化及其与氮素可能存在的相互影响关系,采用三维荧光光谱结合平行因子分析以及相关性分析,以西南某市旱季市政污水处理厂为研究对象,探究DOM荧光组分随工艺单元的变化规律及其与氮素转化的相关性。结果表明:1)市政污水处理厂水体DOM主要由4个荧光组分组成,即类蛋白质组分C1(类酪氨酸)、C2(类色氨酸)和类腐殖质组分C3、C4。污水处理厂进水以类蛋白质组分为主,该组分占总荧光强度比例的平均值为66.5%,其中C1含量较高,其荧光强度占类蛋白质组分比例的平均值为54.6%。最终出水则以类腐殖质组分为主,该组分占总荧光强度比例的平均值为71.7%,而出水的类蛋白质组分中C2含量较高,其荧光强度占类蛋白质组分比例的平均值为99.8%。2)随处理工艺流程的进行,DOM的荧光强度基本呈现逐渐降低的趋势,尤其是C1在工艺流程中荧光强度逐渐趋于0;而类腐殖质组分相对稳定,不随处理流程的进行而变化。3)污水处理厂生物处理单元之后DOM的荧光指数(FI)均大于1.9,表明DOM以转化为自生源为主。4)污水处理厂工艺流程中NH_(4)^(+)-N和溶解性总氮(DTN)与C1组分和腐殖化指数(HIX)之间有良好的相关性,采用多元回归方式可有效预测工艺流程中NH_(4)^(+)-N和DTN的浓度。建议污水处理厂可根据DOM光谱性质与氮素(NH_(4)^(+)-N和DTN)之间的大量数据建立普适模型,对尾水的排放和受纳水体中氮的变化趋势进行预测。

关键词：污水处理厂溶解性有机质(DOM) 特征变化氨氮溶解性总氮(DTN)

铁基秸秆源和牛粪源生物炭对镉砷的吸附特征及机制

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环境科学学报 2024年第9期44卷 283-292页

作者：李珺昭谢炎杰尹英杰王艺晧毛萌商建英中国农业大学土地科学与技术学院农业和农村部华北地区耕地保护重点实验室北京100193 中国科学院南京土壤研究所土壤环境与污染修复重点实验室南京210008

为提高生物炭对镉(Cd)和砷(As)的吸附能力,分别对小麦秸秆生物炭(WBC)和牛粪生物炭(MBC)进行铁改性,得到两种铁基生物炭(Goe@WBC和Goe@MBC).对4种生物炭进行表征分析,并通过批实验考察其对Cd(II)和As(III)的吸附性能.结果表明,铁改性后... 详细信息

为提高生物炭对镉(Cd)和砷(As)的吸附能力,分别对小麦秸秆生物炭(WBC)和牛粪生物炭(MBC)进行铁改性,得到两种铁基生物炭(Goe@WBC和Goe@MBC).对4种生物炭进行表征分析,并通过批实验考察其对Cd(II)和As(III)的吸附性能.结果表明,铁改性后生物炭铁(Fe)元素含量增加,表面出现大量Fe‒O、‒OH/Fe‒OH官能团.与WBC和MBC相比,Goe@WBC和Goe@MBC对Cd(II)的吸附能力分别提高了47%和27%,对As(III)的吸附能力分别提高了903%和940%.与Goe@WBC相比,Goe@MBC表现出对Cd(II)更强、对As(III)更弱的吸附能力,这是因为牛粪富磷,PO_(4)^(3-)可与Cd(II)结合为沉淀,而与As(III)存在竞争吸附.综上,通过在生物炭表面生成铁基,可同时提高其对正电重金属镉(Cd)和负电重金属砷(As)的吸附能力,为修复重金属复合污染提供新途径.

关键词：生物炭铁改性吸附镉砷

The OsAMT1.1 gene functions in ammonium uptake and ammoniumepotassium homeostasis over low and high ammonium concentration ranges

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Journal of Genetics and Genomics 2016年第11期43卷 639-649页

作者： Chang Li Zhong Tang Jia Wei Hongye Qu yanjie xie Guohua Xu State Key Laboratory of Crop Genetics and Germpiasm Enhancement and MOA Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River College of Resources and Environmental Sciences Nanjing Agricultural University Nanjing 210095 China Laboratory Center of Life Sciences College of Life Sciences Nanjing Agricultural University Nanjing 210095 China

Rice （Oryza sativa） grown in paddy fields is an ammonium （NH4^＋）-preferring crop; however, its AMT-type NH4^＋ transporters that mediate root N acquisition have not been well characterized yet. In this study, we analyzed the expression pattern and physiological function of the OsAMT1.1 gene of the AMT1 subfamily in rice. OsAMT1.1 is located in the plasma membrane and is mainly expressed in the root epidermis, stele and mesophyll cells. Disruption of the OsAMTI.1 gene decreased the uptake of NH4^＋, and the growth of roots and shoots under both low NH4^＋ and high NH4^＋ conditions. OsAMT1.1 contributed to the short-term （5 min） ^15NH4^＋ influx rate by approximately one-quarter, irrespective of the NH4^＋ concentration. Knockout of OsAMTI.I significantly decreased the total N transport from roots to shoots under low NH4^＋ conditions. Moreover, compared with the wild type, the osamt1.1 mutant showed an increase in the potassium （K） absorption rate under high NH4^＋ conditions and a decrease under low NH4^＋ conditions. The mutants contained a significantly high concentration of K in both the roots and shoots at a limited K （0.1 mmol/L） supply when NH4^＋ was replete. Taken together, the results indicated that OsAMT1.1 significantly contributes to the NH4^＋ uptake under both low and high NH4^＋ conditions and plays an important role in N-K homeostasis in rice.

关键词： Ammonium Ammonium transporter Interaction Potassium Rice

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植物非生物逆境学科发展综述

植物生理学报 2024年第2期60卷 248-270页

作者：赵杨杨永青丁杨林张蘅谢彦杰赵春钊刘林川王鹏程中国科学院分子植物科学卓越创新中心/上海植物逆境生物学研究中心上海200032 中国农业大学生物学院北京100193 上海交通大学生命科学技术学院上海200240 南京农业大学生命科学学院南京210095 华南农业大学林学与风景园林学院广州510642 南方科技大学前沿生物技术研究院广东深圳518055

干旱、盐碱和极端温度等非生物胁迫严重抑制植物的生长和发育,是影响农业生产的主要环境因素。植物应答环境胁迫的分子机制一直是植物生物学的热点研究领域。本文邀请在非生物胁迫应答分子机制领域非常活跃的青年科学家,简要综述相关研... 详细信息

干旱、盐碱和极端温度等非生物胁迫严重抑制植物的生长和发育,是影响农业生产的主要环境因素。植物应答环境胁迫的分子机制一直是植物生物学的热点研究领域。本文邀请在非生物胁迫应答分子机制领域非常活跃的青年科学家,简要综述相关研究领域近年来的主要研究进展,并展望未来这一领域值得深入研究的一些科学问题,希望能引起对这一领域的更多的关注和思考。

关键词：非生物胁迫感受信号转导分子机制

绿豆GPX基因家族鉴定及其对非生物胁迫的响应

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南京农业大学学报 2024年第3期47卷 453-461页

作者：邱涛涛苏鸿飞王瑜张禾佳胡慧欣阮诗婷袁星星谢彦杰南京农业大学生命科学学院江苏南京210095 江苏省农业科学院经济作物研究所江苏南京210014

[目的]本文利用生物信息学方法从绿豆基因组中筛选谷胱甘肽过氧化物酶(glutathione peroxidase,GPX)基因家族成员,并探究其组织表达模式及其对若干胁迫的响应。[方法]利用生物信息学的方法对绿豆GPX家族的等电点、相对分子质量、共线性... 详细信息

[目的]本文利用生物信息学方法从绿豆基因组中筛选谷胱甘肽过氧化物酶(glutathione peroxidase,GPX)基因家族成员,并探究其组织表达模式及其对若干胁迫的响应。[方法]利用生物信息学的方法对绿豆GPX家族的等电点、相对分子质量、共线性、上游顺式作用元件等进行分析,通过转录组测序和RT-qPCR进一步分析绿豆GPX基因家族的组织表达模式以及在不同逆境胁迫和激素作用下的表达水平。[结果]从绿豆基因组中筛选出8个GPX基因,其不均匀分布于1/2/3/5/10号染色体上。基因结构及基序分析结果显示,8个GPX的基因结构类似,均含有6个内含子区域,并且大部分GPX成员都具有UTR区。转录组以及RT-qPCR分析结果表明,多个GPX家族成员均能响应多种逆境胁迫。组织表达分析结果显示,不同GPX家族成员在不同组织中呈特异性表达;GPX家族整体在绿豆中表达丰度较低,但表达量能被各种胁迫强烈诱导。[结论]绿豆GPX基因家族响应多种非生物胁迫,不同GPX成员响应的胁迫类型不同。

关键词：绿豆 GPX基因家族非生物胁迫基因表达

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社区居民听力状况及其影响因素调查

现代养生 2024年第18期24卷 1398-1401页

作者：刘玉宇谢雁洁黄佳平叶俊威张样聪韶关学院医学院

目的调查分析广东省及省外居民的听力功能状况及影响因素，为预防听力损失提供参考依据。方法选取2023年通过互联网和线下参与听力习惯及功能问卷调查的普通居民为调查对象，共收集有效问卷179份。分析听力功能损失及相关症状的影响... 详细信息

目的调查分析广东省及省外居民的听力功能状况及影响因素，为预防听力损失提供参考依据。方法选取2023年通过互联网和线下参与听力习惯及功能问卷调查的普通居民为调查对象，共收集有效问卷179份。分析听力功能损失及相关症状的影响因素。结果结果显示，有52人（29.05%）经常未注意他人问候，73人（40.78%）听距小于3米，71人（39.66%）需要他人重复说话或加大音量才能听清楚。其中听力功能损失与居住环境、是否常用耳机无关（P>0.05）；听力功能损失与年龄、性别、噪音下使用耳机、护耳习惯、饮食、睡眠情况、运动量、起夜情况有关（P0.05）；听力损失临床表现与性别、起夜情况有关（P<0.05）。结论听力损失与在噪音环境使用耳机、无护耳习惯、睡眠小于7小时、不运动、饮食以荤为主等不良生活习惯相关。建议有条件的学校、街道办、广大媒体可以加强与听力相关的健康教育。

关键词：听力损失用耳习惯听力功能

Hydrogen sulfide, a signaling molecule in plant stress responses

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Journal of Integrative Plant Biology 2021年第1期63卷 146-160页

作者： Jing Zhang Mingjian Zhou Heng Zhou Didi Zhao Cecilia Gotor Luis CRomero Jie Shen Zhenglin Ge Zhirong Zhang Wenbiao Shen Xingxing Yuan yanjie xie Laboratory Center of Life Sciences College of Life SciencesNanjing Agricultural UniversityNanjing 210095China Instituto de Bioquimica Vegetal y Fotosintesis Consejo Superior de Investigaciones Cientfficas y Universidad de SevillaSevilla 41092Spain Institute of Industrial Crops Jiangsu Academy of Agricultural SciencesNanjing 210014China

Gaseous molecules, such as hydrogen sulfide(H_2S)and nitric oxide(NO), are crucial players in cellular and(patho)physiological processes in biological systems. The biological functions of these gaseous molecules, which were first discovered and identified as gasotransmitters in animals, have received unprecedented attention from plant scientists in recent decades. Researchers have arrived at the consensus that H_2S is synthesized endogenously and serves as a signaling molecule throughout the plant life ***, the mechanisms of H_2S action in redox biology is still largely unexplored. This review highlights what we currently know about the characteristics and biosynthesis of H_2S in plants. Additionally,we summarize the role of H_2S in plant resistance to abiotic stress. Moreover, we propose and discuss possible redox-dependent mechanisms by which H_2S regulates plant physiology.

关键词： gasotransmitter hydrogen sulfide persulfidation plant physiology redox signaling stress response

Hydrogen sulfide-linked persulfidation of ABI4 controls ABA responses through the transactivation of MAPKKK18 in Arabidopsis

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Molecular Plant 2021年第6期14卷 921-936页

作者： Mingjian Zhou Jing Zhang Jie Shen Heng Zhou Didi Zhao Cecilia Gotor Luis CRomero Ling Fu Zongmin Li Jing Yang Wenbiao Shen Xingxing Yuan yanjie xie Laboratory Center of Life Sciences College of Life SciencesNanjing Agricultural UniversityNanjing 210095PR China Instituto de Bioquímica Vegetal y Fotosíntesis Consejo Superior de Investigaciones Científicas y Universidad de SevillaAvenida Américo Vespucio 4941092 SevillaSpain State Key Laboratory of Proteomics Beijing Proteome Research CenterNational Center for Protein Sciences BeijingBeijing Institute of LifeomicsBeijing 102206China Institute of Industrial Crops Jiangsu Academy of Agricultural SciencesNanjing 210014China

Hydrogen sulfide(H2S)is a signaling molecule that regulates plant hormone and stress *** phytohormone abscisic acid(ABA)plays an important role in plant adaptation to unfavorable environmental conditions and induces the persulfidation of L-CYSTEINE DESULFHYDRASE1(DES1)and the production of H2S in guard ***,it remains largely unclear how H2S and protein persulfidation participate in the relay of ABA *** this study,we discovered that ABSCISIC ACID INSENSITIVE 4(ABI4)acts downstream of DES1 in the control of ABA responses in ***4 undergoes persulfidation at Cys250 that is triggered in a time-dependent manner by ABA,and loss of DES1 function impairs this ***250 and its persulfidation are essential for ABI4 function in the regulation of plant responses to ABA and the H2S donor NaHS during germination,seedling establishment,and stomatal closure,which are abolished in the ABI4Cys250Ala mutated *** of the ABI4Cys250Ala variant into the abi4 des1 mutant did not rescue its hyposensitivity to ***250 is critical for the binding of ABI4 to its cognate motif in the promoter of Mitogen-Activated Protein Kinase Kinase Kinase 18(MAPKKK18),which propagates the MAPK signaling cascade induced by ***,the DES1-mediated persulfidation of ABI4 enhances the transactivation activity of ABI4 toward MAPKKK18,and ABI4 can bind the DES1 promoter,forming a regulatory *** together,these findings advance our understanding of a post-translational regulatory mechanism and suggest that ABI4 functions as an integrator of ABA and MAPK signals through a process in which DES1-produced H2S persulfidates ABI4 at Cys250.

关键词： hydrogen sulfide persulfidation DES1 ABI4 MAPKXK18 transactivation ABA response