Heme Oxygenase-1 is Associated with Wheat Salinity Acclimation by Modulating Reactive Oxygen Species Homeostasis

作     者：Yanjie Xie Weiti Cui Xingxing Yuan Wenbiao Shen Qing Yang 

作者机构：College of Life Sciences Cooperative Demonstration Laboratory of Centrifuge Technique Nanjing Agricultural University and Beckman Coulter Ltd. Co. Nanjing Agricultural University Nanjing 210095 China The Institute of Vegetable Crops Jiangsu Academy of Agricultural Sciences Nanjing 210014 China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2011年第53卷第8期

页      面：653-670页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081702[工学-化学工艺] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 

基　　金：supported by the Program for New Century Excellent Talents in University(Grant No.NCET-07-0441) the Priority Academic Program Development of Jiangsu Higher Education Institutions,the Education Department of Jiangsu(Grant No.200910) the Technology Support Program in Jiangsu Province,China(Grant No.BE2010382) the National Natural Science Foundation of China(Grant No.30671248 the Fundamental Research Funds for the Central Universities(Grant no.KYJ200912 and KYZ200905) 

主　　题：carbon monoxide haem oxygenase-1 lipid peroxidation ROS homeostasis salinity acclimation wheat. 

摘      要：Heme oxygenase-1 （HO-1） has been recently identified as an endogenous signaling system in animals. In this study, HO-1 upregulation and its role in acquired salt tolerance （salinity acclimation） were investigated in wheat plants. We discovered that pretreatment with a low concentration of NaCl （25 mmol/L） not only led to the induction of HO-1 protein and gene expression, as well as enhanced HO activity, but also to a salinity acclimatory response thereafter. The effect is specific for HO-1, since the potent HO-1 inhibitor zinc protoporphyrin IX blocks the above cytoprotective actions, and the cytotoxic responses conferred by 200 mmol/L NaCl are reversed partially when HO-1 inducer hemin is added. Heme oxygenase catalytic product, carbon monoxide （CO） aqueous solution pretreatment, mimicked the salinity acclimatory responses. Meanwhile, the CO-triggered re-establishment of reactive oxygen species （ROS） homeostasis was mainly guaranteed by the induction of total and isozymatic activities, or corresponding transcripts of superoxide dismutase, ascorbate peroxidase, and cytosolic peroxidase （POD）, as well as the downregulation of NADPH oxidase expression and cell-wall POD activity. A requirement of hydrogen peroxide homeostasis for HO-1-mediated salinity acclimation was also discovered. Taken together, the above results suggest that the upregulation of HO-1 expression was responsible for the observed salinity acclimation through the regulation of ROS homeostasis.